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Citations for Corning :
251 - 300 of 303 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... except the custom hydrogel formulations were replaced with growth factor deficient (GFD) Matrigel basement membrane matrix (9.2 mg/mL protein concentration, Corning, MA, USA) in both layers ...
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bioRxiv - Synthetic Biology 2024Quote: ... NanoLuc activity assays were initiated by adding serially diluted fluorofurimazine to the protein samples prepared in flat white bottom non-binding surface 96-well plates (Corning 3990). The final concentrations of fluorofurimazine ranged from 0.0977 μM to 12.5 μM ...
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bioRxiv - Synthetic Biology 2024Quote: ... FP assays were initiated by adding either SNAP-Cell 647-SiR or SNAP-Surface 549 to the protein samples prepared in flat black bottom non-binding surface 96-well plates (Corning 3991). FP measurements were conducted in 20 second intervals for 80 min at room temperature with a final reaction volume of 100 μL ...
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bioRxiv - Immunology 2020Quote: Human iPSCs were initially transferred from mouse embryonic feeder layers to feeder-free conditions and thereafter maintained on a selected batch of Matrigel® (Corning) in mTeSR™1 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Luciferase-expressing mouse ovarian cancer ID8-Luc2 cells were maintained in high glucose Dulbecco’s Modification of Eagle’s Medium (DMEM; Corning, 10-017-CV), 4% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: Human antibody concentrations in mouse sera after passive immunization was measured by ELISA using 96-well half-area plates (Corning, #3690) coated with 2 µg/ml sheep anti-Human-IgG (ICN ...
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bioRxiv - Cancer Biology 2022Quote: ... NCI-H1048 (H1048) tumor cells at a density of 2.5×106 per mouse in 50% Matrigel (Cat. No. 356237, Corning, Corning, NY) and PBS mixture were injected subcutaneously (s.c. ...
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bioRxiv - Microbiology 2023Quote: ... cell culture vessels were coated with either 1 µg/ml (EVT) or 2.5 µg/ml (STB) of mouse Collagen IV (Corning™ 354233). For EVT differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... the culture was stimulated by substituting one third of the medium with L929 medium (L929 mouse fibroblast cells had previously been plated in T175 cm2 cell culture flasks (Corning, Merck) with 100 mL culture medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were implanted subcutaneously in the mouse flank in a 1:1 ratio of serum-free RPMI medium:extracellular matrix (Matrigel solution, cat# 356234, Corning, Tewksbury, MA) containing 0.5 µg/ml human VEGF 165 (cat# 100-20 ...
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bioRxiv - Cancer Biology 2024Quote: Two million Aska-SS or SYO1 cells were injected subcutaneously into the right flank of each mouse in a mixture of 100 µL PBS and 50% Matrigel (Corning #356234). Once tumors were established ...
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bioRxiv - Molecular Biology 2021Quote: A BioTek Synergy H1 microplate reader was used to analyze expression kinetics or protein stability of dox-preinduced cells seeded confluent (100 % monolayer) in Costar 96 flat bottom plate (Corning™ 3596) ± dox with Leibovitz’s L-15 phenol red-free (CO2 independent media ...
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bioRxiv - Bioengineering 2020Quote: ... A hydrogel consisting of extracellular matrix (ECM) proteins made of a final concentration of 7.0 mg/ml Type I rat tail collagen (Corning, 354249, MA, USA) was used in all experiments ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of the prepared protein standards and lysed MV samples were loaded on a Corning® 96-well plate (Corning, UK). 25 μL working reagent A and 200 μL reagent B were also added ...
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bioRxiv - Biochemistry 2020Quote: ... and 10 μL of protein solution was aliquoted into wells of a 384-well low-volume optical plate (Corning Inc., Corning, New York), and 10 μL of mineral oil was then overlaid onto the protein solution ...
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bioRxiv - Developmental Biology 2020Quote: Wells of 24-well plates were coated with undiluted Matrigel® hESC-Qualified Matrix (protein concentration approximates 10mg/ml)(cat. 354277, Corning®). Matrigel was solidified by incubation for at least 10 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
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bioRxiv - Synthetic Biology 2023Quote: ... The fluorescence intensity (excitation = 485, emission = 510) of 150 μL prey proteins in 96 well plates (Corning® 96 Well CellBIND® Microplates, Corning) using a microplate reader (Agilent BioTek Synergy H4 Hybrid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells expressing fluorescent proteins (mEGFP and mCherry) were plated in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA) and allowed to attach to plates overnight ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in 0.05 M borate buffer (for protein extraction) or in 24-well plates containing 0 thickness glass coverslips (Assistant Cat#01105209 coated with Matrigel (Corning Cat#356235). On DIV1 80% of medium was exchanged to neuron growth medium (5% fetal bovine serum (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were started by adding protein to DNA in a Corning® 384-well Low Volume Black Round Bottom Polystyrene NBS Microplate (Corning #4514). Data are collected for 50 cycles each lasting 20 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... 1×107 pfu of purified baculovirus or 1 μg of NA9 protein were two-fold serial diluted by 1x NA-Flour Assay buffer in black flat-bottom 96-well plates (Corning Inc., NY). Fifty μl of NA-Fluor substrate (Final concentration of 200 μM ...
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bioRxiv - Bioengineering 2023Quote: Bone-marrow derived Sprague-Dawley rat mesenchymal stem cells that express red fluorescent protein (RFP-MSCs, P2, Creative Bioarray, Shirley, NY) were cultured in DMEM with 10% FBS and 1% penicillin-streptomycin (Corning, Corning, NY). The wraps were subjected to ethylene oxide gas sterilization and then seeded with 1 x 105 RFP-MSCs ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated with a 10 kDa membrane (Spin-X® UF 500 µL Centrifugal Concentrator, 10,000 MWCO; Corning, NY, USA) in 20 mM HEPES-KOH buffer (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: Kinetic assays of all protein variants were conducted in 96-Well Black Flat Bottom Polystyrene Non-Binding Surface Microplates (Corning, Lowell, MA) using a plate reader (CLARIOstar ...
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bioRxiv - Cancer Biology 2021Quote: ... 10×106 cells/mouse were suspended in a 1:1 solution of serum-free medium and growth factor reduced Matrigel (Corning, NY, USA). 50 µl of cell suspension were injected orthotopically into the anterior prostate lobe of 10-weeks old CD1-nude male mice (Charles River Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor models of parental B16-F10-luciferase or PD-L1-knockout B16-F10-luciferase cells were established by injecting 7.5 × 104 cells per mouse mixed with an equal volume of Matrigel (Corning Inc., Corning, NY) subcutaneously into the flanks of four-to-six-week-old C57BL6 mice ...
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bioRxiv - Neuroscience 2020Quote: ... and plated on a labtek (Starstedt, Nümbrecht, Germany) (1 mouse brain is needed to seed 1 labtek) previously coated with 2 % matrigel (Corning, Steuben, NY, USA) diluted in DMEM 1 g/L glucose ...
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bioRxiv - Biochemistry 2021Quote: NIH-3T3 mouse embryo fibroblast (NIH-3T3 ATCC® CRL-1658) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning 10-013-CV) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of blood was added from each mouse to each well within a 96-well flat bottom plate (Corning Costar, Corning, New York). A dihydrorhodamine (DHR ...
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bioRxiv - Developmental Biology 2023Quote: Human iPSCs were grown on irradiated mouse embryonic fibroblast (MEF) feeders in hES medium defined as DMEM/F12 medium (Corning, L022046-10092CVR) supplemented with 25% of KnockOut™ Serum Replacement (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: To validate the apoptotic nature of miR-29b delivery to cancer cells human osteosarcoma cells (SaOS2) and mouse osteosarcoma cells (K7M2) were seeded onto 24 well plate with polymer cover slip (Corning, P24-1.5P) at a seeding density of 2×104 cells/cm3 ...
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bioRxiv - Developmental Biology 2023Quote: ... The already established H156 and RUNX1C-EGFP H930 hESCs lines were grown on irradiated mouse embryonic fibroblast (MEF) feeders in hES medium defined as DMEM/F12 medium (Corning, L022046-10092CVR) supplemented with 25% of KnockOut™ Serum Replacement (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The surgery was performed to implant A2780 cells at 5x106 per mouse embedded in 100 ul of Growth Factor Reduced Basement Membrane Matrix (Corning, Cat#354230) into aged rats and young rats ...
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bioRxiv - Genetics 2022Quote: ... the gRNA complex was mixed with Cas9 protein (IDT, 1081059; 10 μg/μl) in 1x phosphate-buffered saline (1xPBS; Corning, 21-040-CV) to final concentrations of 1.5 μM gRNA complex and 0.25 μg/μl Cas9 protein ...
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bioRxiv - Biochemistry 2020Quote: ... and 10 μL of protein solution was aliquoted into wells of a 384-well low-volume optical plate (Corning Inc., Corning, New York), and 10 μL of mineral oil was then overlaid onto the protein solution ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated, Corning, NY). The plate was mixed in the plated reader orbitally at medium speed for 15 s and read at the height of 1.5 mm with a gain of 48 ...
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bioRxiv - Synthetic Biology 2020Quote: HEK 293 cells (ATCC) stably over-expressing full-length human ACE2 protein were seeded in 96 well white polystyrene microplates (Corning, Cat. No. CLS3610) at 0.03×106 cells/well in DMEM (10% FBS and 1% Pen-Strep) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated, Corning, NY). The plate was mixed in the plated reader orbitally at medium speed for 15 s and read at the height of 1.5 mm with a gain of 50 ...
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bioRxiv - Bioengineering 2022Quote: Live mouse chondrogenic cells (ATDC5) (passage 19, 1.0 × 106 cells) were expanded in 100 mm × 20 mm dishes (Corning Inc., New York, USA) using 10 mL DMEM containing 10% FBS and 1% PS and cultured at 37°C under 5% CO2 for 1 week ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 ξ 105 AKTP-v or 1 ξ 106 AP-tdt cells were injected into the NSG mouse spleen with 25 μl of Matrigel (Corning, Corning, NY, USA). Liver tissues were examined histologically at day 7 and 14 after transplantation ...
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bioRxiv - Cell Biology 2024Quote: The AML12 mouse hepatocyte cells were cultured in DMEM/F-12 50/50 mix with L-glutamine and 15 mM HEPES (Corning 10-092-CV) supplemented with 10% heat-inactivated fetal bovine serum (Sigma F4135) ...
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bioRxiv - Bioengineering 2022Quote: Human embryonic kidney HEK293T cells and RAW264.7 mouse macrophage cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning 10-013-CV; Corning, NY, USA) supplemented with 10% EV-depleted fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... The spleen and two lobes of lung tissue were collected from each mouse and placed on ice in RPMI 1640 medium (Corning, #10-040-CM) containing 10% FBS (R10 medium) ...
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bioRxiv - Molecular Biology 2024Quote: ... The liver was cut from the mouse and placed on a pre-chilled 10cm dish containing 20 mL of DMEM (Corning, #90-113-PB). Dissociated liver cells were transferred to a 50 mL conical tube with a 100 μm cell strainer placed over the top (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... to a concentration of 30 nM and 24.5 μL of diluted protein was aliquoted to each well of a black 384 well plate (Corning 384-Well, Flat-Bottom Microplate). Each well was treated with 0.5 μL of compound or vehicle and the plate was incubated for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... treated iMGs were harvested in low-protein binding tubes (fisher scientific; Cat#: 13-864-407) using media and PBS (Corning; Cat#:21-040-CV) to wash off iMGs from cell culture wells ...
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bioRxiv - Biochemistry 2023Quote: ... protein (obtained from Ono Pharmaceuticals) were mixed in PBS and aliquoted into a 96-well v-bottom assay plate (Corning, P-96-450V-C) in a total volume of 20μl ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of blood was added from each mouse to each well within a 96-well flat bottom plate (Corning Costar, Corning, New York). A dihydrorhodamine (DHR ...
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bioRxiv - Immunology 2022Quote: Single-cell suspensions of lymphocytes were generated by standard gentle mechanical disrupting of the mouse spleens in R10 complete medium (RPMI 1640 (Corning, Catalog #10-041-CV) + 10% FBS Hyclone (GE Healthcare ...