Labshake search
Citations for Corning :
2251 - 2300 of 2651 citations for GDNF family receptor alpha 1 GFRA1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: Replicas of silicon molds containing the different features (750nm in height) was made by double-casting PDMS (mixed at 10:1 base and curing agent, Sylgard184, Dow Corning) cured at 80°C for 3 hours ...
-
bioRxiv - Immunology 2019Quote: ... which were subsequently plated (5×104 cells/well) on rat-tail collagen type 1-coated permeable transwell membrane supports (6.5mm; Corning Inc). HAE cultures were grown in B-ALI medium supplemented with inducer (Lonza Inc. ...
-
bioRxiv - Immunology 2021Quote: ... samples were prepared by washing cells twice using FACS buffer (Phosphate-buffered saline (PBS) with the addition of 1% Fetal Bovine Serum (FBS)) in 96 well U-bottom plates (Corning) or round-bottom polystyrene tubes (BD Biosciences) ...
-
bioRxiv - Bioengineering 2021Quote: ... The elastomer base of the PDMS was thoroughly mixed with the curing agent in a 10:1 ratio as reported by the datasheet of the manufacturer (Sylgard 184, Dow Corning). Air bubbles were removed from the uncured polymer applying vacuum to the mix for 2 hours or until no bubbles were visible ...
-
bioRxiv - Systems Biology 2021Quote: ... supplemented with 10% Fetal Bovine Serum (FBS; Hyclone) and 1% penicillin-streptomycin (100 U/mL and 100 μg/mL; Corning). Cells were incubated at 37°C in 5% CO2.
-
bioRxiv - Bioengineering 2021Quote: ... was prepared at a ratio of 1:10 curing agent (Sylgard 184 silicone elastomer curing agent, 3097358-1004, Dow Corning) and degassed in a vacuum for 30 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Cal85-1 cells were plated in 96-well tissue-culture treated assay plates (Corning; Cat. No. 3904; Corning, NY) and incubated overnight at 37°C and 5% CO2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Cal85-1 cells were plated in 96-well tissue-culture treated assay plates (Corning; Cat. No. 3904; Corning, NY) and incubated overnight at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: Whole cellular clusters (30 x106 cells for proteomics and 1 x106 cells for RNA sequencing) were collected and washed with pre-chilled PBS (-Ca2+/-Mg2+, Corning), snap frozen (−80°C ...
-
bioRxiv - Microbiology 2021Quote: ... Calu-3 cells were also obtained from ATCC and cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/glutamine (Corning). Both cell lines were maintained at 37°C 5% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... The pellet was then resuspended in 1 mL sterile water and transferred to a 1.5 mL twist cap tube (Corning # 4309309). Spores were plated on BHIS +Taurocholate agar plates to determine concentration of stock ...
-
bioRxiv - Microbiology 2021Quote: ... Primary cells derived from single patient sources were expanded on plastic and subsequently plated (3.3×104 cells / well) on rat-tail collagen type 1-coated permeable transwell membrane supports (6.5 mm, #3470; Corning, Inc.). The immortalized HAE line BCi-NS1.1 was kindly provided by Drs ...
-
bioRxiv - Microbiology 2021Quote: ... coli BW25113 harboring either BAC-pks or the empty BAC were centrifuged (16,100 g and 1 min) and the supernatant was passed through a 0.22 μm filter (Corning Spin-X). Growth of non-colibactin producing E ...
-
bioRxiv - Cell Biology 2021Quote: ... Pre-lubricated pipette tips (Maxymum Recovery™, Axygen®,1-200μL and 100-1000μL) and microcentrifuge tubes were purchased from Corning Life Sciences.
-
bioRxiv - Immunology 2020Quote: ... Whole blood packs/buffy coats were diluted 1 in 2 with sterile PBS onto 15mL Lymphocyte Separation Medium (25-072-CV LSM, Corning). Cells were separated by centrifugation at 20C for 20 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were split at a 1:12 ratio and seeded on 6-well plates coated with 75 μg/mL (growth factor reduced) Matrigel (Corning) on day −1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Dissociated samples were filtered through a 40µm filter and resuspended in FACs buffer (PBS/ 10%FBS/1% Penstrep) before filtering through a FACs tube (Corning #352235). Single cells were sorted into 384-well-cell-capture plates containing barcoded primers from Single Cell Discoveries (https://www.scdiscoveries.com/ ...
-
bioRxiv - Bioengineering 2022Quote: ... with a thin layer of PDMS prepolymer comprising of PDMS base monomer and curing agent (10:1 w/w; Sylgard 184, Dow-Corning). PDMS coating layer was then thermally cured at 110 °C for at least 24 h ...
-
bioRxiv - Biochemistry 2022Quote: ... Heart pieces were then allowed to sediment for 1 min and the supernatant containing suspended cardiac myocytes was transferred to a new tube containing 1/10 volume of cardiomyocyte medium (DMEM supplemented with 10% FBS, 10% Nu serum (Corning), 1x Penicillin-Streptomycin (Gibco) ...
-
bioRxiv - Bioengineering 2022Quote: ... The PDMS membrane was then coated with 1 ml of 50 μg/ml rat collagen I (Corning, Corning, NY, USA) overnight in the incubator of 37 °C and 5% CO2 ...
-
bioRxiv - Bioengineering 2022Quote: ... The PDMS membrane was then coated with 1 ml of 50 μg/ml rat collagen I (Corning, Corning, NY, USA) overnight in the incubator of 37 °C and 5% CO2 ...
-
bioRxiv - Biochemistry 2022Quote: ... 350ug of the plasmids encoding spikes were transfected into 1L HEK-293F cells at 1 million cells/ml using Transfectagro (Corning) and 40K polyethylenimine (PEI ...
-
bioRxiv - Cell Biology 2022Quote: ... pre-mixed EV binding solution (table 1) was added to each well of 96 well V-bottom plate (Corning, 3897) or a clear PCR plate ...
-
bioRxiv - Microbiology 2022Quote: ... This was followed by three cycles of freeze–thaws on dry ice and 200 μL of this passage 1 (P1) stock was transferred to 25 mL of fresh Sf9 suspended cell culture in polycarbonate Erlenmeyer flasks (Corning) at the density of 2.5 × 106 cells/mL ...
-
bioRxiv - Microbiology 2022Quote: ... Clone E6-1 was obtained from ATCC (TIB-152) and cultured in suspension in RPMI1640 medium (Corning #10–040 CM), supplemented with 10% heat inactivated FBS (Sigma-Aldrich #14009C ...
-
bioRxiv - Microbiology 2022Quote: ... cells were diluted to an OD600 of 0.1 for both types of assays and 148 μL was added into wells of a 96-well black plate (Corning). Fluorescence was recorded for 5 mins at 1 min intervals at 37°C (BioTek Neo ...
-
bioRxiv - Neuroscience 2022Quote: ... 2x106 cells were replated onto POLFN-coated 6-well plates (0,01% of Poly-L-ornithine, Sigma, #P4957; 5 µg/ml Laminin; 1 µg/ml Fibronectin, Corning, #354008) in NES medium containing DMEM/F-12 supplemented with B-27 (1:1000) ...
-
bioRxiv - Neuroscience 2022Quote: ... or hemizygous doubly transgenic ΔNLS pups at postnatal day 1–3 were dissected in cold PBS to remove meninges and dissociated by manual trituration with a P1000 pipette in 1 ml fresh culture media consisting of high-glucose DMEM (Corning), 20% heat-inactivated FBS (VWR #89510-188) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mice were subcutaneously injected in the right flank with 1×106 KPCY-derived cells in 50% PBS and 50% Matrigel basement membrane matrix (#354234, Corning). In the interventional experiments ...
-
bioRxiv - Immunology 2022Quote: ... Isolated hpT and dpT cells (1×106 cells per well) were seeded in Seahorse XF24 designated plates using Cell-Tak (Corning) adherent and assayed according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... hTERT RPE-1 cells of all genotypes were cultured in DMEM/F-12 (with L-glutamine and 15 mM HEPES, Corning) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... for 1 hour at room temperature followed by washing three times in PBS and gently lifted using a cell scraper (Corning). These suspensions were pelleted by centrifugation at 300x g for 3 min and resuspended in a 500 µL of PBS ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cultures were then diluted 1:100 in fresh LB medium (200 μL) to load a microplate (96 wells, black, clear bottom; Corning) with the appropriate concentrations of lactose (0 ...
-
bioRxiv - Bioengineering 2024Quote: ... PDMS was fabricated by mixing a 10:1 mass to mass ratio of Sylgard 184 elastomer with the initiator (Dow Corning). PDMS spacers are removed from the mold ...
-
bioRxiv - Biochemistry 2024Quote: ... were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4.5 g/L glucose and 1 mM sodium pyruvate (Corning 15-013-CV), with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... and after adding 1 mM EDTA to stop the reaction passing the cells through a 100µm cell strainer (Corning, 431752) twice ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Pellet was resuspended in 5 mL and 1 mL of resuspended pellet was added to a T25 flask (Corning #430639) containing 10 mL of media.
-
bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
-
bioRxiv - Immunology 2024Quote: Lung tissues were gently dissociated in small fragments (1-5 mm length) using scissors then placed in 6 wells plates (Corning). To generate pericytes ...
-
bioRxiv - Immunology 2024Quote: Neutralization assays used 25 µl of pseudovirus and 25 µl of serially diluted monoclonal antibodies or IgG purified from sera for 1 hr at 37°C in a full-area 96-well tissue culture polystyrene microplate (Corning). After incubation ...
-
bioRxiv - Immunology 2024Quote: ... Macrophages and target cells were cocultured at a 5:1 E:T ratio for 3 hours at 37 °C in ultra-low attachment 96-well plates (Corning 7007), unless otherwise indicated ...
-
bioRxiv - Microbiology 2024Quote: U937 monocytes or macrophages were seeded at 1×105 in 96-well black-wall clear-bottom plates (Corning, cat# 3904) in phenol red-free DMEM supplemented as indicated for each assay ...
-
bioRxiv - Microbiology 2024Quote: ... of virus and plasma samples in duplicate at a 1:30 dilution were combined in a 96-well U-bottom plate (Corning) and incubated at 37°C for 2 h ...
-
bioRxiv - Immunology 2024Quote: ... BMDCs (1×106 cells) were dispersed in RPMI medium with 1% BSA and seeded into 5 μm pore size transwell inserts (Corning) of a 24-well tissue culture plate containing 500 ml RPMI/BSA medium with CCL4 (R&D ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by subcutaneous injection of 1×106 K562-shNC or K562-shRAPSYN #3 cells in 60 μL Matrigel (354234, Corning) into the right foreleg ...
-
bioRxiv - Microbiology 2023Quote: ... fabrum C58C1 with different pGinger vectors were grown overnight in 10mL of LB supplemented with kanamycin overnight at 30°C with 250 rpm shaking and then diluted 1:100 into 500 μL of fresh LB media with kanamycin in a deep-well 96-well plate (Corning) For inducible promoters ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... glass-bottomed Mattek dishes coated with poly-L-lysine were further coated in 1 mg/mL laminin in H20 (Corning) for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... 3D cultures were washed with 1 mL of PBS following removal of media and then depolymerised using 1 mL of ice-cold cell recovery solution (Corning) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... and passaged at least two times before plating (7×104 cells/well) on rat-tail collagen type-1 coated permeable transwell membrane supports (6.5mm; Corning Inc) to generate human airway epithelial cultures (HAE) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Table 1) was designed and synthesized as previously reported.15 The compounds were printed onto 384-well plates (Corning 3570) in 10 concentration points (10 ...