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Citations for Corning :
1551 - 1600 of 1694 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI) of 2-50 in Dulbecco’s Modified Eagle Medium (Corning, Corning, NY) supplemented with 10% HyClone Characterized Fetal Bovine Serum (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... 10-7) were added to 10 mL of 2% Tryptic soy agar (TSA; Oxoid) inside Falcon round-bottom polypropylene tubes (Corning Inc.). The temperature was examined using a laboratory digital thermometer (Thermco ...
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bioRxiv - Immunology 2024Quote: ... Neutralized bovine collagen I (2 mg/ml) was plated 40 µl per well in a black 96-well plate with flat clear bottom (Corning/Merck). The plate was kept at 37°C with 5 % CO2 for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were resuspend in neutralized bovine collagen I (2 mg/ml) and plated in a black 96-well plate with flat clear bottom (Corning/Merck) at a density of 25,000 cells/40 µl per well ...
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bioRxiv - Neuroscience 2023Quote: ... 1 or 2 days old hermaphrodite adults were glued (Histoacryl Blue, Braun, Germany) to a sylgard (Sylgard 184, Dow Corning, USA)-coated cover glass covered with bath solution ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellet resuspended in 80% growth factor-reduced basement matrix (either Matrigel®, Corning, 356231; or BME-2®, AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL by depositing at least six 40 μL drops at the bottom of a non-tissue culture treated plate ...
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bioRxiv - Cell Biology 2023Quote: ... and re-plated at a density of 1 to 2×105 per cm2 in growth factor reduced Matrigel (Corning Incorporated, Corning, NY) coated plates in mTeSR ...
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bioRxiv - Microbiology 2023Quote: ... and 2-fold serially diluted in 25 µL of PBS in duplicate in a 96-well v-bottom plate (Corning, #3897). A/CA/04/09 virus was adjusted to 4 hemagglutination units (HAU ...
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bioRxiv - Microbiology 2022Quote: ... The resulting single-cell suspensions of bacteria at 2×106 cells/well were placed in the bottom of wells of a Cell-Tak (Corning, cat. No. 354240, Corning, NY)-coated XF culture plate (Agilent/Seahorse Biosciences ...
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bioRxiv - Immunology 2022Quote: ... streptavidin (Jackson Immuno Research Labs cat.# 016-000-084) was coated at 2 μg/ml in PBS onto 96-well half-area high binding plates (Corning, 3690) overnight at 4°C ...
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bioRxiv - Zoology 2022Quote: ... the 66 largest individuals (~1-2 mm long) were removed from the bulk culture in the finger bowls and raised in 6-well plates (Corning, #3736), grouped by size into 6 sub-groups of 11 individuals each (Fig ...
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bioRxiv - Microbiology 2023Quote: Caco-2 cells (1 × 106 cells/well) were seeded onto 6-Transwell insert culture plates (Corning, Lowell, MA, USA, cat# 3412). RAW264.7 macrophages were seeded onto 6-well flat-bottom cell culture plates at 1 × 105 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: Subcutaneous ovarian tumors were initiated by injecting 2×106 ID8p53-/- cells suspended in 150μl of 50% Matrigel (Corning Life Science, Massachusetts) in the subcutaneous layer of the left flank of the mice ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 cells were seeded in top chambers of regular or Matrigel-coated trans-wells (migration – Corning #353097; invasion – Corning #354480) in 400 μl of 0.5% FBS-containing medium for 3 hours before migration/invasion towards medium containing 10% FBS or 100 ng/ml IGF-1 in lower chambers ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 cells were seeded in top chambers of regular or Matrigel-coated trans-wells (migration – Corning #353097; invasion – Corning #354480) in 400 μl of 0.5% FBS-containing medium for 3 hours before migration/invasion towards medium containing 10% FBS or 100 ng/ml IGF-1 in lower chambers ...
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bioRxiv - Microbiology 2023Quote: ... serine 2 (TMPRSS2) expressing (Vero-ACE2/ TMPRSS2) cells (15) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Corning, 10-013-CV) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: ... vortexed for 2 min and centrifuged for 1 min at 2000 g using a table top centrifuge (Corning Mini Centrifuge, Corning). 15 µl of the aqueous solutions were extracted from the phase separated liquids for osmolality measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 105 LX-2 cells were seeded in the top chamber of an 8 μm pore migration cassette (Corning, Kaiserslautern, Germany) and were allowed to migrate for 4,5 h ...
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bioRxiv - Bioengineering 2024Quote: ... for acute drug sensitivity measurement via CTG and plated at 75k cells per well with 3 mL of DMEM+2%FBS in 6-well ultra-low attachment plates (Corning, 3471) for fxSMR assays.
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bioRxiv - Genomics 2024Quote: ... cells were washed an additional 2 times with 500 μL T-ChIC wash buffer before passing through a 70 mm cell strainer (Corning, 431751) for sorting.
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bioRxiv - Cancer Biology 2024Quote: ... then DNA was denatured with 2 N hydrochloric acid with 0.5% Triton-X in phosphate-buffered saline (Corning, # 46-013-CM). Neutralization with sodium tetraborate was followed by incubation with anti-BrdU/FITC antibody (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 1× 106 cells were electroporated with 2 µg of replicon RNA and subsequently plated into 96-well plates (Corning; 3904) with a seeding density of 1x104 cells/well ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture was seeded at a density of 105 cells in a 9:1 (Caco-2) ratio on Transwell clear inserts with 0.4 µm pore polyester membranes (Corning, NY, USA) and incubated for 28 days at 37°C in a 5% CO₂ atmosphere ...
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bioRxiv - Microbiology 2024Quote: ... the cells were infected with virus in 0.4 ug/mL polybrene by spinoculation at 2500 rpm for 2 hours at room temperature in the 6-well culture plate (Corning Incorporated) with virus equivalent of 25ng of p24 per 3 x 106 CD4+ cells per well ...
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bioRxiv - Neuroscience 2024Quote: preMG were harvested as described “Harvesting microglia precursor cells (preMG),” and 2×105 cells were transferred into one well of a 24-well (Corning, #3527) containing 3:1-medium supplemented with 50 ng/mL MCSF ...
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bioRxiv - Synthetic Biology 2024Quote: ... were diluted 1:100 into 100 µl 2×YT in a 96-well flat-bottom transparent microtiter plate (Corning Inc., USA). To assess growth kinetics ...
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bioRxiv - Cell Biology 2024Quote: ... cell suspensions were adjusted to 30×106 per 2 mL and dispensed in 5mL FACS tubes with cell strainer snap cap (Corning, 352235). Replicate 1 was sorted on FACS Aria II and replicate 2 on a BD Influx System ...
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bioRxiv - Cancer Biology 2024Quote: MCF10a and MDA-MB231 spheroids were formed starting from a culture of 20,000 cells per spheroid mixed with their medium and a 2% Matrigel (Corning Matrigel Matrix) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Molecular Biology 2024Quote: ... Human milk samples (2 µL) were diluted with 48 µL glycerol lysis solution from the kit in white-walled 96-well plates (Corning 3903) and incubated for 30 minutes at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... excess solution was removed and a 1-2 mm thick polydimethylsiloxane (PDMS) slab (SYLGARD 184 silicone elastomer kit, Dow Corning, USA) previously incubated in a plasma cleaner (PDC-32G-2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells used in experiments were never allowed to grow beyond 80% confluency and regularly split every 2 or 3 d using 0.05% Trypsin/0.53 mM EDTA in HBSS (Corning, 25-051-CL). Cells were typically cultured on tissue culture treated plastic (SCBT ...
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bioRxiv - Cancer Biology 2022Quote: ... renal cell carcinoma call lines were obtained from ATCC and maintained at 37°C and 5% CO2 in RPMI 1640 (Corning, Cat# 10-040-CV) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Systems Biology 2020Quote: ... were cultured at 37 ◦C at concentration 5% CO2 atmospheric on a 96-well plate in RPMI1640 cell growth medium (Corning, Cat.10-040-CVR). For stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated astrocytes were placed 5×104 cells/well in a 24 well place for 7 days with DMEM (Corning Incorporated, Nova York, EUA) (high glucose ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting pellet was suspended with 15 mL of DMEM supplemented with 10% Fetal Bovine Serum (FBS) and Penicillin-Streptomycin and incubated at 37°C and 5% CO2 for 4 hours in a T75 flask (Corning Life Sciences, MA, USA) to eliminate fibroblasts and mononuclear cells (as fibroblasts and mononuclear cells get adhere to flask) ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in serum-free RPMI-1640 medium complemented as described before and were seeded (5–6 × 107 PBMCs/plate) in 100 mm × 20 mm cell culture-treated polystyrene culture dishes (Corning, New York, NY, USA). Cultures were maintained in a humidified atmosphere at 37°C with 5% CO2 for 1 h ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at a density of 3.0×106 cells/well for 7 days at 37 °C and 5% CO2 in ultra-low attachment six-well plates (Corning Life Sciences, Tewksbury, MA) to allow the differentiation of monocytes into macrophages ...
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bioRxiv - Immunology 2023Quote: ... Tissue was separated in 8 mL 37% Isotonic Percoll (Percoll: Cytiva, #17-0891-02; RPMI 1640: Corning, #10-040-CV, supplemented with 5% FBS) by centrifugation at 1200xg for 30 minutes with a slow break ...
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bioRxiv - Microbiology 2023Quote: Vero 5×103 cells/well in 200 µL of CGM were seeded onto 96-well flat bottomed polystyrene plates (Corning, USA, Cat No. 3598) to obtain 80% confluent monolayers after 24 hours’ incubation at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... in freely behaving mice using an internal cannula (C315IS-5/SPC; P1 Technologies) attached to a Hamilton syringe by silicone tubing (2415500; Dow Corning Corporation, Midland, MI, USA). CNO and vehicle injections occurred during Zeitgeber time (ZT ...
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bioRxiv - Genetics 2023Quote: ... Nuclei were strained through the 35 μm filter on the Falcon round bottom 5 ml polystyrene test tubes (Corning Catalog No. 08-771-23) and sorted with use of the fluorescence activated cell sorter FACSAriaIII (20 psi ...
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bioRxiv - Bioengineering 2022Quote: ... into the perfusable channels at a density of 5 × 106 cells mL−1 with a supporting collagen type I matrix (Corning, 1 mg mL−1). The constructs were cultured in a co-culture medium (1:1 mix of osteogenic medium and EGM-2).
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bioRxiv - Microbiology 2024Quote: ... Cells were grown at 37°C in a 5% CO2 water-saturated atmosphere in 25 or 75 cm2 flasks (Corning Inc, Corning, NY, USA) and diluted to 3×105 cells/mL in RPMIc every 3 days ...
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bioRxiv - Immunology 2024Quote: ... Tissue was separated in 8 mL 37% Isotonic Percoll (Percoll: Cytiva, #17–0891-02; RPMI 1640: Corning, #10–040-CV, supplemented with 5% FBS) by centrifugation at 1200xg for 30 minutes with a slow brake ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant was then collected by spinning the cells for 5 min at 3000g and filtered through a 28mm syringe filter (Corning, 431229, 0.2 µM PES). TIFM media control was subjected to the same incubation ...
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bioRxiv - Biochemistry 2024Quote: 293T cells (DSMZ, cat# ACC-635) were grown at 37°C under a humidified atmosphere holding 5% CO2 in DMEM (Corning, cat# 15-013-CV) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were passed through a 40 µm cell strainer and collected in 5 mL round-bottomed polystyrene tubes (Corning Life Sciences, ref. 352235). Cell suspensions were transferred to 2.0 mL LoBind Tube (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... six-step serial 1:2 dilutions of compound solutions were prepared in DMSO using solvent-resistant polypropylene microplates (cat. 3357, Corning, Corning, NY). Concentration ranges (125-3.91 nM ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in PBS to a concentration of 2 x 107 cells/ml and passed through 100 µm cell strainer (Corning Inc., USA). Fixation for 5 min at room temperature was performed by adding an equal volume of PBS 2% methanol-free formaldehyde (Thermo Scientific ...
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bioRxiv - Genetics 2021Quote: ... The part of the biopsy destined to be used for single fiber analysis was placed in Krebs–Ringer buffer containing 0.1% procaine hydrochloride for 2–3 min and pinned down at resting length in a Petri dish coated with Sylgard 184 (Dow Corning, Midland, MI). It was then fixed with 2% depolymerized paraformaldehyde and 0.15% picric acid in 0.1 M phosphate buffer at room temperature for half an hour ...