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Citations for Corning :
1001 - 1050 of 1172 citations for Recombinant Human Lipocalin 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... MuSCs were cultured in 2 mL myogenic growth medium (GM) containing 43% Dulbecco’s Modified Eagle’s Medium (DMEM; Corning Cellgro # 10-013), 40% Ham’s F-10 (Corning Cellgro # 10-070-CV) ...
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bioRxiv - Bioengineering 2020Quote: ... The cell pellet was resuspended in FACS buffer (PBS containing 2% FBS) and replaced into a 96-well Clear Round Bottom Ultra-Low Attachment Microplate (Corning, #7007). The cell solution was centrifuged again at 300 rcf for 3 min at 4°C to pellet the cells ...
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bioRxiv - Cell Biology 2021Quote: ... semiconfluent MYOD1-hiPSCs maintained under feeder-free conditions were dissociated into single cells by Accutase and plated onto 24-well plates coated with growth factor-reduced Matrigel for 2 hours at a dilution of 1:50 (Corning, USA) at a density of 7 x 104 cells/well in StemFit medium (AK02N ...
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bioRxiv - Neuroscience 2020Quote: ... media37 with 2% FBS before seeding 0.1×106 cells per 500 μL in a well of a 24-well plate (Corning Cat. #353847). The cells were maintained in TIC media at 37 °C and 10% CO2 with media change every other day until the day (around 10-14 div ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hESCs were counted and 1.5 to 2 million cells were plated per well of a 12-well plate coated with matrigel (Corning Catalog #354277). Cells were grown for 1 day with Stem Differentiation Neuronal induction medium (Stem Cell Technologies Catalog #05835) ...
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bioRxiv - Neuroscience 2020Quote: ... CAD5 Prnp+/+ and CAD5 Prnp-/- cells (5 × 104 in 2 ml of medium) were seeded into 6-well plates (Corning Costar) and cultured for 1–2 days before exposure to 500 µg/ml of prion-infected mouse brain homogenate ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK293T cells were cultured in Dulbecco’s modified Eagle’s (DMEM) medium with high glucose and 2 mM L-glutamine (Corning; 25-005-CI). Ls174T-W4 cells (a generous gift from Dr ...
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bioRxiv - Neuroscience 2020Quote: ... The final 100 μl were resuspended in 1 ml of PBS with 2% FBS and filtered through a pre-washed 40 μm Falcon filter (Corning, 352340). Cells were pelleted at 650 g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The final 100 μl were resuspended in 1 ml of PBS with 2% FBS and filtered through a pre-washed 40 μm Falcon filter (Corning, 352340) with an additional 5 ml of PBS with 2% FBS ...
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bioRxiv - Immunology 2021Quote: ... 56 to evaluate antibodies binding to SARS- CoV-2 RBD were performed by coating of high-binding 96-half-well plates (Corning 3690) with 50 μl per well of a 1μg/ml protein solution in Phosphate-buffered Saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... expanded HAE cells from 3 donors at passage 2 were combined (3 × 104 cells per donor) and were seeded on 12-well transwell membranes (Corning Costar), which were coated with a mixture of BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Mammary tissue was shaken in collagenase medium (2 mg /mL collagenase type IV, Sigma C5138, in DMEM / F12, Corning 10-090) at 37°C for 1 hour ...
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bioRxiv - Biophysics 2020Quote: Turbidity of samples at varying [NaCl] were represented by OD500 measured using a plate reader (Bio-Tek Synergy 2) and 384-well microplate (Corning 3844). To minimize the inference of ThT fluorescence and tau fibril scattering ...
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bioRxiv - Immunology 2022Quote: Enzyme-Linked Immunosorbent Assays (ELISAs)35,36 to evaluate antibodies binding to SARS-CoV-2 RBD were performed by coating of high-binding 96-half-well plates (Corning 3690) with 50 μl per well of a 1μg/ml protein solution in Phosphate-buffered Saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were resuspended in 4mL FACS Buffer (2% BSA, 10µM Y-27632, 100U/mL penicillin-streptomycin) and transferred into 5 mL FACS tubes (Corning, Cat#352063). Cells were centrifuged again at 300g for 3 minutes at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were resuspended in 4mL FACS Buffer (2% BSA, 10µM Y-27632, 100U/mL penicillin-streptomycin) and transferred into 5 mL FACS tubes (Corning, Cat#352063). Cells were centrifuged again at 300g for 3 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then trypsinized and seeded on porous polyester membranes (2-3× 104 cells per membrane) in cell culture inserts (Transwell-clear, diameter 12 mm, 0.4 μm pores; Corning, Acton, MA). Five days later the culture medium was removed from the upper compartment and the epithelium was allowed to differentiate by using the differentiation medium consisting of 1:1 DMEM (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 2 μl of Magnatec 10W-40 oil (Castrol) were pipetted into each well of a 96-well plate lid (Corning incorporated) and were allowed to equilibrate for 2 hours to ensure that each well was evenly coated ...
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bioRxiv - Microbiology 2021Quote: ... and 1% P/S were incubated with 25 µl of SARS-CoV-2 PSV at 37°C for 1 h in 96-well half-well plate (Corning, 3688). After incubation ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μL of each dilution was then added to cells seeded a day prior at 3×103 cells in a volume of 40 μL of MEM-2% FCS per well in a 384-well plate (Corning #CLS3985). The plates containing the cells and compounds were incubated for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... CEM-A T cells in complete RPMI medium (10% fetal bovine serum; 2 mM L-glutamine; 1% penicillin/streptomycin, and 1% hypoxanthine/thymidine; Corning, USA) were seeded on 25 mm No ...
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bioRxiv - Immunology 2022Quote: ... to evaluate antibodies binding to SARS-CoV-2 RBD or NTD were performed by coating of high-binding 96-half-well plates (Corning 3690) with 50 μl per well of a 1μg/ml protein solution in Phosphate-buffered Saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with the appropriate panel of antibodies for 30 minutes at 4°C and washed 2-3 times with PBS (Corning, Corning, NY) and analyzed through flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... they were plated into 96-well plate (20,000 cells/well) on a matrigel-coated surface with media containing 2% matrigel (Corning, NY, USA). BO-264 was added 72 hrs after seeding ...
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bioRxiv - Cell Biology 2022Quote: ... 3D migration was performed in 2 mg/ml collagen gel polymerized at 37°C (rat tail collagen type I, Corning 354263), with the cells sandwiched between the two layers of collagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... Overnight cultures of 43 mutant clones isolated with the CytoFlex Index sorting function were inoculated as 1% starter culture in 2 ml LB medium (in 14 ml flacon snap cap tubes; Corning, USA) induced at an OD600 of 0.6 to 0.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... spheroids were collected and pelleted at 100x g for 3 minutes before being re-suspended in a gel mix solution consisting of 2 mg/mL Collagen I (Corning, 354236) and 17.5% Matrigel (Corning ...
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bioRxiv - Systems Biology 2019Quote: ... all three cell lines were cultivated in batch mode by seeding 2×105 cells/mL into orbitally agitated disposable Erlenmeyer flasks (Corning, USA) in duplicates ...
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bioRxiv - Developmental Biology 2019Quote: Operations were done in 35 mm plastic petri dishes with 2 mm thick silicon-coated bottoms (SYLGARD–184, Dow Corning, Inc.) on cydippids 1.5-3.0 mm in diameter ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 3-[2-(N,N-diethyl-N-methylammonium)ethyl]-7-methoxy-4-methylcoumarin (AMMC, 100 μM; Cyp2d10; Cat. No. 451700, Corning Inc.), 7-methoxy-4-(trifluoromethyl)-coumarin (MFC ...
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bioRxiv - Biochemistry 2020Quote: ... was added to 20 μL of 15% pyrene labeled actin and incubated for 2 minutes in 96-well flat bottom black polystyrene assay plates (Corning 3686). To initiate the reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... C2C12 BRE-Luc and 293T CAGA-Luc cells were seeded at 20,000 cells in DMEM supplemented with 2% FBS per well in tissue culture treated 96-well plates (Costar® 3610; Corning). The cells were incubated for 1 hour (37°C and 10% CO2 ...
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bioRxiv - Immunology 2021Quote: ... and 1% P/S were incubated with 25 µl of SARS-CoV-2 PSV at 37°C for 1 h in 96-well half-well plates (Corning, 3688). After the incubation ...
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bioRxiv - Neuroscience 2019Quote: ... The MV pellet was gently resuspended in 2% BSA and passed through a 70 μm filter (Falcon, Corning Incorporated, NY, USA). The MVs were placed into a 1.5 mL tube ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 500 μL of 2% BSA/PBS and filtered through a 35-μm pre-separation filter (Corning, USA.). The fluorescence intensity was measured using a BD Accuri flow cytometer (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... the cell pellet was resuspended in 500 μL of 2% BSA/PBS and filtered through a 35-μm pre-separation filter (Corning, USA.). The CD73 positive cells were collected as photoreceptor cells using an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mL of resuspended cells was transferred into a 2 mL cryogenic vial (430659, Corning Life Sciences Plastic, Lowell, MA, USA) and frozen overnight at -80 °C in a NALGENE Cryo 1 °C freezing container (5100-0001 ...
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bioRxiv - Neuroscience 2021Quote: ... the CNS was dissected from a larva and placed in a droplet of external saline solution on a coverslip coated with a thin layer (1-2 mm) of cured SYLGARD Elastomer (Dow-Corning, USA). The external saline solution was composed of 135 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 x 104 primary fibroblasts were seeded in a 6.5 mm trans-well with 0.4 μm pore (Corning, New York, USA). After the three days incubation time ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 and Hs578T cells (2 x 105) were seeded onto a 6-well Clear Flat Bottom Ultra- Low Attachment plate (Corning, 3471) in complete RPMI medium ...
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bioRxiv - Neuroscience 2021Quote: ... The brain surface at the location of the penetration was covered with 2% agarose and then with silicone elastomer (Sylgard 184, Dow Corning, USA) to prevent drying ...
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bioRxiv - Immunology 2020Quote: ELISAs to evaluate antibodies binding to SARS-CoV-2 RBD and trimeric spike proteins were performed by coating of high binding 96 half well plates (Corning #3690) with 50 μL per well of a 1μg/mL protein solution in PBS overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Parasite titrations were performed by serial dilution (1:2 dilutions) of tissue homogenates in 96-well flat-bottom microtiter plates (Corning, Corning, NY) in M199 cell culture media in duplicate and incubated at 26°C without CO2 for 7–10 days ...
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bioRxiv - Bioengineering 2021Quote: ... 104/μl of target (Vero E6, CaCo-2 and CaLu-3) cells were plated in a 96-well flat bottom plate (Corning, #3917). 75 μg/ml D-firefly luciferin potassium salt (Biosynth ...
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bioRxiv - Microbiology 2022Quote: ... The resulting single-cell suspensions of bacteria at 2×106 cells/well were placed in the bottom of wells of a Cell-Tak (Corning, cat. No. 354240, Corning, NY)-coated XF culture plate (Agilent/Seahorse Biosciences ...
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bioRxiv - Immunology 2022Quote: ... streptavidin (Jackson Immuno Research Labs cat.# 016-000-084) was coated at 2 μg/ml in PBS onto 96-well half-area high binding plates (Corning, 3690) overnight at 4°C ...
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bioRxiv - Zoology 2022Quote: ... the 66 largest individuals (~1-2 mm long) were removed from the bulk culture in the finger bowls and raised in 6-well plates (Corning, #3736), grouped by size into 6 sub-groups of 11 individuals each (Fig ...
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bioRxiv - Microbiology 2023Quote: Caco-2 cells (1 × 106 cells/well) were seeded onto 6-Transwell insert culture plates (Corning, Lowell, MA, USA, cat# 3412). RAW264.7 macrophages were seeded onto 6-well flat-bottom cell culture plates at 1 × 105 cells per well ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellet resuspended in 80% growth factor-reduced basement matrix (either Matrigel®, Corning, 356231; or BME-2®, AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL by depositing at least six 40 μL drops at the bottom of a non-tissue culture treated plate ...
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bioRxiv - Microbiology 2023Quote: ... and 2-fold serially diluted in 25 µL of PBS in duplicate in a 96-well v-bottom plate (Corning, #3897). A/CA/04/09 virus was adjusted to 4 hemagglutination units (HAU ...