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Citations for Corning :
851 - 900 of 1210 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... cells were plated at a density of 3,000 cells per well in 96-well assay plates (Corning). ATP bioluminescence was assessed at 13 days after gene knockout with the CellTiter-Glo Luminescent Cell Viability Assay (Promega) ...
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bioRxiv - Biophysics 2021Quote: ... Dorsal skin pieces were dissected from E6 embryos and spread flat on the Polycarbonate membrane (pore size: 0.4 μm, Corning, Cat.No.3413). Culture is DMEM with 2% chick serum and 10% FBS along with pharmacological agents NSC23766 (Abmole ...
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bioRxiv - Cell Biology 2020Quote: ... This was seeded on wells coated with the Matrigel basement membrane matrix for feeder-free culturing (Corning Inc., Corning, NY, USA) and then cultured in the undifferentiated maintenance medium Essential 8 (E8 ...
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bioRxiv - Cell Biology 2020Quote: ... This was seeded on wells coated with the Matrigel basement membrane matrix for feeder-free culturing (Corning Inc., Corning, NY, USA) and then cultured in the undifferentiated maintenance medium Essential 8 (E8 ...
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bioRxiv - Genetics 2021Quote: ... the membrane was washed with PBS with 0.1% Tween 20 (PBS-T) once quickly and with TBS (Corning, 46-012-CM) with 0.1% Tween 20 (TBS-T ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... Freshly isolated mTEC were seeded at a density of 1×105/cm2 on supported semi-permeable polyester membranes (Transwell, Corning-Costar, Corning, NY) that had previously been coated with collagen ...
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bioRxiv - Cell Biology 2020Quote: ... 100 µL of medium was removed from each well and replaced with 100 µL of Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix (356230, Corning), diluted to the final concentration of 5 mg/mL in ice-cold serum-free DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... Intestinal crypts were isolated from colon removed for nonmalignant etiology using a previously established protocol.[61] Isolated crypts were embedded in Matrigel (Basement membrane, Corning, 354248), and cultured in 24-well plates (Polystyrene ...
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bioRxiv - Cancer Biology 2021Quote: 100’000 MC-38 cells were seeded in 500 μl DMEM + 1% FCS atop of Corning BioCoat Matrigel Invasion Chambers with 8 μm PET Membrane (Corning, #354480) and chemotaxis was induced by presence of DMEM + 10% FCS in the bottom chamber ...
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bioRxiv - Cell Biology 2021Quote: ... hTEC cells were expanded in-vitro and allowed to differentiate into ciliated cells using air-liquid interface (ALI) conditions on supported membranes (Transwell, Corning Inc., Corning, NY), as previously described (PMID ...
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bioRxiv - Microbiology 2020Quote: ... expanded HAE cells from 3 donors at passage 2 were combined (3 × 104 cells per donor) and were seeded on 12-well transwell membranes (Corning Costar), which were coated with a mixture of BSA ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 cells (3 x 106) were plated on 75 mm transwell inserts with a polycarbonate membrane pore size of 3 µm (Corning, 3420) that allows media and gas exchange during compression ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 x 106) were plated on a 75 mm transwell insert with a polycarbonate membrane pore size of 3 µm (Corning, 3420). Before seeding ...
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bioRxiv - Cell Biology 2020Quote: ... a master mix of cells resuspended in sterile phosphate-buffered saline (PBS) and 50% final concentration of Matrigel Membrane Matrix solution (Corning 354234) was prepared such that 1×106 cells were delivered in 150 μL of solution ...
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bioRxiv - Cell Biology 2022Quote: ... we developed a protocol where solution changes could be done without centrifugation using 24-well plate inserts with an 8 micron pore membrane at the insert base (Corning 3422). Before use with aggregates ...
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bioRxiv - Genomics 2021Quote: ... 107 cells were resuspended in HCM supplemented with 1% defined FBS and mixed with equal volume of Matrigel (growth factor reduced basement membrane matrix, Corning #356231) and injected subcutaneously into the flank of a 6-week old immunodeficient BALB/c nude male mice (Scanbur) ...
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bioRxiv - Developmental Biology 2021Quote: The chemotactic response of mandible myoblasts to CXCL12 was evaluated using a 24-well fitted transwell inserts with membranes (8 μm pore size, Corning Falcon). The inserts were then coated with Corning Matrigel matrix (Fisher scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then trypsinized and seeded on porous polyester membranes (2-3× 104 cells per membrane) in cell culture inserts (Transwell-clear, diameter 12 mm, 0.4 μm pores; Corning, Acton, MA). Five days later the culture medium was removed from the upper compartment and the epithelium was allowed to differentiate by using the differentiation medium consisting of 1:1 DMEM (Invitrogen ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... WT Cas9 expressing PDX366 cells were resuspended in 150 μL Matrigel ®Growth Factor Reduced Basement Membrane Matrix (Corning, Corning, NY). After the anesthetize ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... WT Cas9 expressing PDX366 cells were resuspended in 150 μL Matrigel ®Growth Factor Reduced Basement Membrane Matrix (Corning, Corning, NY). After the anesthetize ...
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bioRxiv - Bioengineering 2020Quote: ... Transwells were coated with a mixture (prepared on ice) of 300 µg/ml Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix (Corning 354230) and 50 µg/ml Collagen I Rat Tail High Concentration (Corning 354249 ...
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bioRxiv - Cancer Biology 2021Quote: Cell migration was analyzed in Corning Transwell Permeable Supports with polycarbonate membranes (24-well plate, 8 μm pore size) (cat#18320044, Corning, Corning, NY). ESCC cells were harvested after trypsin digestion ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 × 106 cells of Capan2 cells were inoculated subcutaneously into the left and right shoulder (1:1 phosphate-buffered saline [PBS]/Matrigel Basement Membrane Matrix High Concentration, Corning, Corning, USA). Tumors were allowed to grow for two to four weeks (tumor volume > 100 mm3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 5×104 cells of each condition were plated in 100 μL DMEM media without FBS in 6.5 mm Transwell chambers with 8.0 mm Pore Polyester Membrane Insert (Corning Inc., Corning, NY), and transferred to a 24-well dish containing 500 μL fresh media (0.5% FBS containing DMEM ...
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bioRxiv - Biochemistry 2020Quote: ... cells were seeded overnight in passaging media on the outer facing part of the filter of transwell inserts (12 mm Transwell®, 0.4 µm Pore Polyester Membrane Insert, Corning, 3460). The transwells were kept inverted in 6-well flat bottom plates to ensure cell settling (Corning CoStar ...
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bioRxiv - Cancer Biology 2020Quote: ... WT Cas9 expressing PDX366 cells were resuspended in 150 μL Matrigel ®Growth Factor Reduced Basement Membrane Matrix (Corning, Corning, NY). After the anesthesia ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cells were plated at 900 cells/ml on Transwell 6-well plates with permeable 24 mm polyester membrane inserts (Corning, 3450) or regular tissue culture-treated plates ...
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bioRxiv - Cancer Biology 2021Quote: ... NSG mice were injected into the right flank with 5000 cells in 50 μL of 25% Matrigel Basement Membrane Matrix (Corning, #354248). Tumor growth was monitored every 3-4 days and the largest diameter was recorded ...
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bioRxiv - Microbiology 2021Quote: ... nucleatum in CDM in the lower chamber of a 6-well Transwell system with 0.4-μm pore polystyrene membrane inserts (Corning, NY, USA), into which 1.4×1010 cells of S ...
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bioRxiv - Immunology 2020Quote: ... neutrophils were separated from splenocytes by placing them in a HTS Transwell-96 permeable device with 0.4 μm pore and polycarbonate membrane and adapted receiver plate (Corning, reference CLS3381). To test the role of MHC-II and CD11b molecules in the suppression mechanism ...
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bioRxiv - Microbiology 2021Quote: ReNcell human neural progenitor cells were seeded at the density of 5×105 cells per well in 6-well plates coated with Matrigel Matrix Basement Membrane (Corning, USA) diluted 1:100 in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... WT Cas9 expressing PDX366 cells were resuspended in 150 μL Matrigel ®Growth Factor Reduced Basement Membrane Matrix (Corning, Corning, NY). After the anesthesia ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded on 12-wells inserts (0.4 μm pore size polyester membrane, 0.5 million cells per transwell; Corning, Corning, NY, USA), precoated with PureCol (30 ug/mL ...
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bioRxiv - Microbiology 2022Quote: ... The bacteriocytes were transferred to 6.5-mm Transwell dishes with 8-µm pore polycarbonate membrane inserts (Corning, New York, NY, USA) and fixed in 4% paraformaldehyde (PFA in phosphate buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 25 μL compound and 75 μL membrane with overexpressed receptor were added to 96-well white round bottom polystyrene NBS microplates (Corning #3605) and incubated on 25°C plate warmer for 90 minutes ...
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bioRxiv - Developmental Biology 2024Quote: 2D EMCs were derived from 3D mSIOs as described15 with the following specifications/exceptions: A 1:40 (vol:vol) mixture of Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning, cat.# 356231) in DMEM/F-12 without phenol red was used for coating imaging dishes ...
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bioRxiv - Cancer Biology 2024Quote: ... released from the basement membrane using a recovery solution – including Dispase II (1 mg/mL) – seeded in a neutralized collagen type-I solution (Corning, 354249) and cultured for additional 24 hours before fixing them with 4% paraformaldehyde (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... multilayer Matrigel constructs were made using the same methods described above except replacing the custom hydrogel formulations with Growth Factor Reduced Basement Membrane Matrigel in both layers (9.2 mg/mL protein concentration, Corning, MA, USA).
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bioRxiv - Bioengineering 2024Quote: ... except the custom hydrogel formulations were replaced with growth factor deficient (GFD) Matrigel basement membrane matrix (9.2 mg/mL protein concentration, Corning, MA, USA) in both layers ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Caco-2 and HT29-MTX were mixed in a 9:1 ratio and 2 × 105 cells total was seeded on 12 mm Snapwell inserts (tissue culture treated polyester membrane, Corning, USA) for ∼2 weeks to form a polarised monolayer ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded on the underside of a tissue culture insert comprised of a polycarbonate membrane with 3 μM pores (Falcon, Corning, NY) and allowed to adhere for four hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Both cell lines were maintained in six-well plates coated with Matrigel growth factor reduced basement membrane matrix (GFR-Matrigel, Corning, 354230) in mTeSR plus medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Migration capacity of homotypic and heterotypic cultures was measured using 6.5 mm Transwell® with 8.0 μm Pore Polycarbonate Membrane Insert (Corning, Madrid, Spain). The transwells were coated with 100 μL of growth factor reduced Matrigel (10 ug/μL ...
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bioRxiv - Microbiology 2023Quote: ... then seeded (3.3 × 104 cells / well) on rat tail collagen type 1-coated permeable Transwell® membrane supports (6.5 mm; Cat No. 3470, Corning, Inc.). Cultures were differentiated in Pneumacult-ALI medium (Cat No ...
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bioRxiv - Biophysics 2024Quote: The ability of melanoma cells to penetrate through a mechanical barrier was conducted using porous inserts (polycarbonate membrane with a pore diameter of 8.0 µm, Corning Costar Transwell), according to the protocol described in Supplementary Material 1.5.
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bioRxiv - Microbiology 2024Quote: ... and plated onto filters (Transwells®, 6.5 mm insert, 24 well plate, 0.4 μm polyester membrane, Corning Incorporated, Kennebunk, ME, USA) coated with collagen IV (Millipore Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture was seeded at a density of 105 cells in a 9:1 (Caco-2) ratio on Transwell clear inserts with 0.4 µm pore polyester membranes (Corning, NY, USA) and incubated for 28 days at 37°C in a 5% CO₂ atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... and AdKLF6-overexpressing HPAECs were grown to confluence in 6.5 mm Transwell inserts with 1.0 µm pore size transparent PET membrane (Corning, Cat. No 353104) in EGM2 culture media containing serum and growth factors ...
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bioRxiv - Neuroscience 2024Quote: ... 200.000 astrocytes were seeded into a geltrex-coated bottom well of a 12 mm Transwell® with 3.0 µm Pore Polycarbonate Membrane Insert plate (Corning, 3402). Cells were either seeded in normal astrocyte medium or astrocyte medium supplemented with 5 mM NAC ...
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bioRxiv - Microbiology 2024Quote: ... Differentiation of the BCi-NS1.1 was performed in 12 mm trans-well plates with 0.4 µm pore polyester membrane inserts (Corning Inc., NY, USA). Before plating ...