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Citations for Corning :
851 - 900 of 918 citations for 7 Hydroxy 4 methylcoumarin 3 acetic acid succinimidyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... cells were initially activated at 4 x 105 - 106 cells/mL in B cell activation media: Iscove’s Modified Dulbecco’s Medium (IMDM, Corning Inc., Corning, NY) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were than trypsinized and seeded into Transwell filters (6.5 mm diameter, 4 μm pore size, Corning Costar, Kaiserslauten, Germany). Filters ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells (TCam-2 or 2102EP) were re-suspended in 500 μl of 4°C cold matrigel (Corning, Amsterdam, Netherlands) and injected subcutaneously into the flank of Crl:NU-Foxn1nu mice (Charles River Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue is again triturated x5 with a 10 ml pipette and x3-4 with a 5 ml pipette before being strained through a 40-micron basket filter (Corning, 431750) and transferred to a 50 ml conical tube ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were then incubated for 48 hours at 4°C with 0.8 mg/mL CellTak adhesive (Corning Life Sciences, Glendale, AZ) diluted in 0.1M NaHCO3 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were suspended in either 4 mg/mL rat tail collagen I or growth factor reduced Matrigel™ (Corning Life Sciences) solutions ...
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bioRxiv - Cell Biology 2022Quote: Purified human full-length LRP1 (10 nM in 100 μl of TNC) was coated overnight at 4 °C onto microtiter plates (Corning, NY). Wells were blocked with 3% BSA in TNC (1 h ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with appropriate antibodies for 30 minutes at 4°C and washed twice with phosphate-buffered saline (PBS, Corning, Corning, NY) and analyzed by flow cytometry (BD ...
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bioRxiv - Neuroscience 2021Quote: ... The iPSC lines used in this study (NSB2607-1-4 and NSB553-S1-1)75,76 were maintained in Matrigel (Corning, cat.# 354230) coated 6-well plates under NPC medium (DMEM/F12 (Life Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... DDM (n-dodecyl β-D-maltoside) in PBS at 4 °C for 24 h followed by coating with Matrigel hESC-qualified matrix (Corning) diluted to 1.3% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... the cells were pelleted again by centrifugation at 1,500 rpm for 5 minutes at 4°C and re-suspended in Leibovitz’s L-15 (Corning 10-045-CV) media ...
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bioRxiv - Microbiology 2021Quote: ... SIGLEC1 or ACE2 were resuspended at 4×106 cells/mL and 100 μL per well were seeded onto V-bottom 96-well plates (Corning, 3894). The plate was centrifuged at 2,000 rpm for 5 minutes and washed with PBS (pH 7.4) ...
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bioRxiv - Cancer Biology 2020Quote: 50 cells were seeded per well in 96-well plates and incubated overnight in their appropriate maintenance medium supplemented with 4% matrigel (Corning, 356231). The next day ...
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bioRxiv - Immunology 2021Quote: ... for 7 minutes at 300 x g (4°C) following sterile filtration through a 70μm cell strainer (Falcon® 70μm Cell Strainer; Corning Inc., NY) and successive wash steps with 1 X phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... and biofilms formed for 4 or 24 hours at 37°C in flat-bottom wells of 96-well plates (Corning, UK). Appropriate media controls were included on each plate to test for contamination ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 on Poly-L-lysine and optionally beaded (for 4-beam SIM) coverslips placed in 6 well plates (Corning, 3506).
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bioRxiv - Microbiology 2022Quote: ... 150 μL of each set of supplemented inoculum solution was dispensed into at least 4 consecutive wells of a 96 well sterile non-binding polypropylene microplate (Corning #3879) and topped with 50 μL sterile light mineral oil to prevent evaporation ...
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bioRxiv - Microbiology 2022Quote: ... 1 x 108 CFUs in 100 μl of PBS were incubated overnight at 4°C in 96-well plates (Corning, NY), washed three times with PBST (Tween 20 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded onto Corning 6-well transwell inserts (10 μm thick polyester inserts with 0.4 μm pore size, 4*106/cm2 pore density, Corning, Wiesbaden, Germany) in 125.000/cm2 in epithelial cell medium (EpiCM ...
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bioRxiv - Immunology 2024Quote: ... The chips were then coated at 4°C overnight with a solution of 30 µg/mL of type I collagen (Corning, #354236) and 100 µg/mL Matrigel (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... and 4×104 (HeLa) were seeded into 24-well Transwell chambers (insert 6.5-mm diameter, 8-μm pore size; Corning Costar Corp.), loaded with DMEM containing 10% FKS ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were spun down at 1,500 x g for 5 minutes and resuspended at 4 x 105 cells/100uL of RPMI-1640 medium (Corning, 45000-396) containing 20% FBS (Omega Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Hydrogel solution was mixed and deposited as 200 μL gels in 4-well chamber slides (Corning, Corning, NY, USA, Cat# 354104), and allowed to solidify at 37°c for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... The mixture was then centrifuged for ten minutes at 12,000 x g at 4°C and then the aqueous top layer was transferred to a 50 mL conical tube (Corning Falcon). SM buffer was added to bring the total volume up to 4.5 mL and mixed with 2.25 g of Cesium chloride (CsCl) ...
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bioRxiv - Cancer Biology 2023Quote: ... 24-month-old WT mice and 4-month-old K14-Cre Bcl11bfl/fl mice were sorted into the 96-well Ultra-Low attachment culture plate (Corning, 3474) (10,000 cells/200μL/well ...
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bioRxiv - Developmental Biology 2023Quote: ... Hydrogel solution was mixed and deposited as 200 μL gels in 4-well chamber slides (Corning, Corning, NY, USA, Cat# 354104), and allowed to solidify at 37°c for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells (derived from a bone marrow biopsy of a metastatic neuroblastoma of a 4-year-old female) were cultured in Dulbecco’s Eagle’s medium (DMEM) (Corning # 10-013-CM) and NB7 cells in RPMI 160 medium (Corning # 10-040-CM) ...
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bioRxiv - Immunology 2021Quote: ... 0.1 mM EDTA) for 3 mins before quenching the reaction with four volumes of complete RPMI media: RPMI 1640 (Corning Cellgro, 50-020-PC), 10% heat-inactivated FBS (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: Calu-3 human adenocarcinoma airway epithelial cells (HTB-55™, ATCC®, USA) were cultured in alpha minimum essential medium (αMEM, Corning®, USA) supplemented with fetal bovine serum (10% ...
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bioRxiv - Microbiology 2019Quote: NCI-60 cell lines were seeded by groups of cell origin at 3 × 104 cells per well in 96-well plates (Greiner, 655948 for Operetta; Corning, 3604 for flow cytometry) and grown overnight in RPMI-1640 medium (Lonza ...
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bioRxiv - Neuroscience 2024Quote: ... a 0.3mg/ml Camptothecin solution was prepared freshly every day of treatment by diluting the 1mg/ml stock solution 1:3 in PBS (Corning; Cat#:21-040-CV). For control mice ...
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bioRxiv - Microbiology 2023Quote: ... The lung conditioned media (diluted 1:20 in RPMI 1640 media) were added to the outer chamber of 24-well transwell plates (3 µm Costar, Corning Inc, New York, USA). Neutrophils and monocytes were seeded at 3 x 105 to 5 x 105 cells/well in the upper chamber of a 24-well Transwell plate and incubated for 2 h at 37°C in a CO2 incubator ...
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bioRxiv - Cancer Biology 2024Quote: For the Transwell migration assay, 3×104 (HGC-27, GES-1) /1×105 (MKN7, MKN74) cells were added to the upper chamber (Corning Incorporated, Corning, NY, USA) in 200 μL FBS-free medium ...
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bioRxiv - Developmental Biology 2021Quote: ... the cell-laden collagen hydrogel was prepared by mixing 150 μl of a 4 mg/ml rat tail type I collagen (Corning, NY, USA) solution ...
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bioRxiv - Biophysics 2020Quote: ... micro-spheroids were formed by trypsinizing and embedding MCF-10A cells within an interpenetrating network (IPN) consisting of 5 mg/ml Alginate (FMC Biopolymer) and 4 mg/ml Matrigel (Corning, No. 354234) as previously shown (16 ...
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bioRxiv - Biophysics 2020Quote: ... that was coated with a mixture of 4 % nitrocellulose in amyl-acetate nitrocellulose and silicon adhesive (3140 RTV Coating, Dow Corning, Midland, USA). The free end of the bundle was pushed to the coating using a microneedle installed on a separate manipulator ...
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bioRxiv - Immunology 2022Quote: ... post activation and were plated onto Seahorse cell plates at 4 × 105 cells per well coated with Cell-Tak (CB-40241; Corning, Reinach, Switzerland) to enhance T cell attachment ...
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bioRxiv - Immunology 2020Quote: ... to remove extracellular bacteria and were incubated with fresh RPMI-1640 supplemented with 2% fetal bovine serum (Seradigm, Radnor, PA) and 4 mM L-glutamine (Corning, Manassas, VA) and treated with either chemical inhibitors or solvent controls ...
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bioRxiv - Immunology 2020Quote: ... seeded at a density of 3.3 × 105 cells/well in 6-well plates were in RPMI-1640 supplemented with 2% fetal bovine serum (Seradigm, Radnor, PA)and 4 mM L-glutamine (Corning, Manassas, VA). Commercially available ON-TARGET plus Non-targeting and CHM SMARTpool siRNA (Dharmacon ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with appropriate antibodies for 30 minutes at 4°C and washed twice with phosphate-buffered saline (PBS, Corning, Corning, NY) and analyzed by flow cytometry (BD ...
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bioRxiv - Microbiology 2021Quote: ... macrophage-like cells from anywhere between passages 4 and 10 are plated at ∼60% confluency on 150 mm non-cell culture treated dishes (Corning® #430597) and activated with 150 U/mL interferon gamma and 10 ng/mL lipopolysaccharide overnight ...
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bioRxiv - Immunology 2020Quote: ... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in 6-well plates at 37°C in 5% CO2 using growth media consisting of 4:1 of DMEM/M199 (Corning; Corning, NY, USA) supplemented with 15% fetal bovine serum (Avantor® Seradigm ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was centrifuged at 14000 x g at 4 °C and filtered through 0.22 μm acetate filters (Corning, Corning, NY; Cat: 8160), lyophilized and stored at -80 °C for subsequent analysis.
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bioRxiv - Biochemistry 2023Quote: ... Aliquots (4 µl) of supernatant samples were placed in 96-well clear flat bottom ultra-low attachment microplates (Corning, New York, US), and 200 µl of Bradford dye reagent were added ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in a 4-well imaging dish (10,000 cells/well) and supplemented with 1% Pen/Strep (Corning, 30-001-C1) to avoid bacterial contamination during long-term acquisition ...
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bioRxiv - Developmental Biology 2023Quote: ... hPSCs were expanded on new MEF for additional 4/5 days and then cultured on Matrigel-coated plasticware (Corning Life Sciences, 356230) for 24-48 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Neutrophils were plated at 4 × 104 cells per well in a 96-well tissue culture plate precoated with Cell-Tak (Corning, no. 354240) and spun at 200g for 1 min with minimal acceleration/deceleration ...
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bioRxiv - Cancer Biology 2023Quote: ... Each of these was then centrifuged at 300g for 5 minutes at 4°C and the respective pellets were re-suspended in 7mg/ml Matrigel (Corning cat#35234) and plated into 24 well plates ...
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bioRxiv - Microbiology 2023Quote: Ferrets were lightly sedated with 4% inhaled isoflurane and inoculated with virus diluted in phosphate-buffered saline (PBS) (Corning 21-040-CV) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Corning 30-002 CI) ...