Labshake search
Citations for Corning :
801 - 850 of 885 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and incubated with a mixture of primary antibodies in the cell incubator (Corning) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1% BSA) and a serial dilution of HQ461.The protein-compound mix was transferred to 384-well plates (Corning 3572); 15 μL per well ...
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bioRxiv - Microbiology 2021Quote: ... was set up at 6-8 mg/mL in loading buffer containing 0 or 500 mM NaCl as 2 μL crystallization drops (1 μL protein: 1 μL reservoir solution) in 96-well plates (Corning) using commercial Classics II ...
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bioRxiv - Cancer Biology 2020Quote: ... cells transfected with pWZL-p53-R175H to overexpress mutant p53 variant R175H (FNEmutp53-R175H) and green-fluorescence protein (GFP) were seeded in ultra low-adhesion plates (Corning). 2% Matrigel was added to the suspended culture after 24 hours to support basement membrane adhesion ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was eluted with Lysis buffer supplemented with 2.5 mM desthiobiotin and concentrated (10k MWCO Corning Spin-X concentrators) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... DNA and DNA binding proteins were incubated for 30 minutes at room temperature and then transferred to a 384 well plate (Corning). Fluorescence anisotropy was measured using an excitation wavelength of 500 nm and an emission wavelength of 577 nm on a Tecan Spark plate reader fitted with a 50% dichroic 510 mirror ...
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bioRxiv - Synthetic Biology 2022Quote: Excitation and emission spectra of the fluorescent proteins were conducted using a BMG Clariostar Plus microplate reader in sealed plates (Corning, 3370 ...
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bioRxiv - Neuroscience 2024Quote: ... The viral supernatant was filtered through a 0.45 µm cellulose acetate low protein binding filter (Corning, Cat # 09-761-35) and stored at 4°C for 2 to 24 hours before further processing ...
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bioRxiv - Microbiology 2024Quote: ... were set up for crystallization as 2 µl crystallization drops (1-µl protein:1-µl reservoir solution) in 96-well (Corning) plates using commercially available Classics II ...
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bioRxiv - Microbiology 2024Quote: The optical density (OD600) measurements for small protein overexpression and deletion constructs were performed in a clear flat bottom 96-well microplate (Corning) containing 150 µL culture per well using a microplate reader (Agilent BioTek Synergy Neo2S) ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs of passages four to five were used and cultured in plates coated with bovine collagen type I protein (354231, Corning). All cell lines were grown in a humidified chamber at 37°C supplied with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: Exosomes pellets were stained with 100μl of an antibody cocktail in 1X PBS (Corning) containing anti-CD9 (human HI9a ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... Recombinant SARS-CoV-2 S protein (10 ng/well) was coated onto a 96-well plate (Costar assay plate, Corning, 3369) for 24 h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 5,000 cells after three hours phagocytosis assay in the presence of 200 nM hybrid protein were dispensed in a 96-well plate (Corning #3596) at a total volume of 100 µL in serum-free and phenol red-free DMEM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were prepared at room temperature using 0.3 μM labeled protein within the bulk of unlabeled materials and loaded into 96-well plates (Corning nonbinding surface). Sample buffer consisted of 50 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Mixtures (200 μL) containing YjoB and target proteins in buffer A were placed onto a 96-well microtiter plate (Corning Incorporated). Absorbance at 340 nm was recorded for 150 min with 3 min intervals using a SpectraMax iD5 microplate reader (Molecular Devices ...
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bioRxiv - Physiology 2022Quote: ... samples containing 30 μg (liver) or 15 μg (gonad) of protein were placed in a solid black 96-well plate (Corning 3912), then the reaction was initiated out of direct light by the addition of buffer containing lucigenin and NADPH ...
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bioRxiv - Microbiology 2020Quote: ... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 5 μg/ml RBD-SpyTag protein produced in our lab was coated onto 96-well high binding Costar® Assay plates (CORNING) at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ELISAs to evaluate antibodies binding to SARS-CoV-2 RBD and trimeric spike proteins were performed by coating of high binding 96 half well plates (Corning #3690) with 50 μL per well of a 1μg/mL protein solution in PBS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Peroxidase activity was measured by diluting 20 uL of sample protein extract with 20 uL of 10 mM PBS (pH 6.0) in a 96-well plate (Costar, Corning, Kennebunk, ME). Then ...
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bioRxiv - Immunology 2023Quote: ... was measured by diluting 20 uL of coral protein extract in 20 uL of 50 mM PBS (pH 7.0) in a 96 well plate (Costar, Corning, Kennebunk, ME). In order to convert prophenoloxidase to phenoloxidase ...
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bioRxiv - Bioengineering 2024Quote: ... multilayer Matrigel constructs were made using the same methods described above except replacing the custom hydrogel formulations with Growth Factor Reduced Basement Membrane Matrigel in both layers (9.2 mg/mL protein concentration, Corning, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells expressing fluorescent proteins (mEGFP and mCherry) were plated in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA) and allowed to attach to plates overnight ...
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bioRxiv - Microbiology 2024Quote: ... Purified NS1 protein from D2Y98P (10 ng/well) diluted in PBS was coated onto 96-well enzyme immunoassay plates (Corning Costar) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein sample was concentrated to a volume of 250 µl using CORNING SPIN-X concentrators (50 kDa cutoff) (Corning, 431490), followed by centrifugation 21,130 g for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... except the custom hydrogel formulations were replaced with growth factor deficient (GFD) Matrigel basement membrane matrix (9.2 mg/mL protein concentration, Corning, MA, USA) in both layers ...
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bioRxiv - Synthetic Biology 2024Quote: ... NanoLuc activity assays were initiated by adding serially diluted fluorofurimazine to the protein samples prepared in flat white bottom non-binding surface 96-well plates (Corning 3990). The final concentrations of fluorofurimazine ranged from 0.0977 μM to 12.5 μM ...
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bioRxiv - Synthetic Biology 2024Quote: ... FP assays were initiated by adding either SNAP-Cell 647-SiR or SNAP-Surface 549 to the protein samples prepared in flat black bottom non-binding surface 96-well plates (Corning 3991). FP measurements were conducted in 20 second intervals for 80 min at room temperature with a final reaction volume of 100 μL ...
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bioRxiv - Bioengineering 2023Quote: An anti-HPC4 monoclonal mouse antibody was coated onto 96-well ELISA plates (9018, Corning) at 5 ug/ml ...
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bioRxiv - Molecular Biology 2021Quote: A BioTek Synergy H1 microplate reader was used to analyze expression kinetics or protein stability of dox-preinduced cells seeded confluent (100 % monolayer) in Costar 96 flat bottom plate (Corning™ 3596) ± dox with Leibovitz’s L-15 phenol red-free (CO2 independent media ...
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bioRxiv - Bioengineering 2020Quote: ... A hydrogel consisting of extracellular matrix (ECM) proteins made of a final concentration of 7.0 mg/ml Type I rat tail collagen (Corning, 354249, MA, USA) was used in all experiments ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of the prepared protein standards and lysed MV samples were loaded on a Corning® 96-well plate (Corning, UK). 25 μL working reagent A and 200 μL reagent B were also added ...
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bioRxiv - Biochemistry 2020Quote: ... and 10 μL of protein solution was aliquoted into wells of a 384-well low-volume optical plate (Corning Inc., Corning, New York), and 10 μL of mineral oil was then overlaid onto the protein solution ...
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bioRxiv - Developmental Biology 2020Quote: Wells of 24-well plates were coated with undiluted Matrigel® hESC-Qualified Matrix (protein concentration approximates 10mg/ml)(cat. 354277, Corning®). Matrigel was solidified by incubation for at least 10 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
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bioRxiv - Synthetic Biology 2023Quote: ... The fluorescence intensity (excitation = 485, emission = 510) of 150 μL prey proteins in 96 well plates (Corning® 96 Well CellBIND® Microplates, Corning) using a microplate reader (Agilent BioTek Synergy H4 Hybrid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells expressing fluorescent proteins (mEGFP and mCherry) were plated in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA) and allowed to attach to plates overnight ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in 0.05 M borate buffer (for protein extraction) or in 24-well plates containing 0 thickness glass coverslips (Assistant Cat#01105209 coated with Matrigel (Corning Cat#356235). On DIV1 80% of medium was exchanged to neuron growth medium (5% fetal bovine serum (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were started by adding protein to DNA in a Corning® 384-well Low Volume Black Round Bottom Polystyrene NBS Microplate (Corning #4514). Data are collected for 50 cycles each lasting 20 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... 1×107 pfu of purified baculovirus or 1 μg of NA9 protein were two-fold serial diluted by 1x NA-Flour Assay buffer in black flat-bottom 96-well plates (Corning Inc., NY). Fifty μl of NA-Fluor substrate (Final concentration of 200 μM ...
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bioRxiv - Bioengineering 2023Quote: Bone-marrow derived Sprague-Dawley rat mesenchymal stem cells that express red fluorescent protein (RFP-MSCs, P2, Creative Bioarray, Shirley, NY) were cultured in DMEM with 10% FBS and 1% penicillin-streptomycin (Corning, Corning, NY). The wraps were subjected to ethylene oxide gas sterilization and then seeded with 1 x 105 RFP-MSCs ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated with a 10 kDa membrane (Spin-X® UF 500 µL Centrifugal Concentrator, 10,000 MWCO; Corning, NY, USA) in 20 mM HEPES-KOH buffer (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: Kinetic assays of all protein variants were conducted in 96-Well Black Flat Bottom Polystyrene Non-Binding Surface Microplates (Corning, Lowell, MA) using a plate reader (CLARIOstar ...
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bioRxiv - Cancer Biology 2020Quote: ... recombinant protein diluted in PBS or SUIT2 conditioned media was immobilized overnight on high binding ELISA 96-well microplates (Corning, cat. no. 9018). The wells were then washed three times with 200 µL PBS with 0.05% Tween-20 (PBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... and 10 μL of protein solution was aliquoted into wells of a 384-well low-volume optical plate (Corning Inc., Corning, New York), and 10 μL of mineral oil was then overlaid onto the protein solution ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated, Corning, NY). The plate was mixed in the plated reader orbitally at medium speed for 15 s and read at the height of 1.5 mm with a gain of 48 ...
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bioRxiv - Synthetic Biology 2020Quote: HEK 293 cells (ATCC) stably over-expressing full-length human ACE2 protein were seeded in 96 well white polystyrene microplates (Corning, Cat. No. CLS3610) at 0.03×106 cells/well in DMEM (10% FBS and 1% Pen-Strep) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated, Corning, NY). The plate was mixed in the plated reader orbitally at medium speed for 15 s and read at the height of 1.5 mm with a gain of 50 ...
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bioRxiv - Bioengineering 2022Quote: PGT121 monoclonal antibody was concentrated via spin filtration (Corning® Spin-X® UF 5kDa MWCO) at 3030 RPM until desired volume recovery ...