Labshake search
Citations for Corning :
651 - 700 of 908 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Equal numbers of single cells (1,500 viable cells per well) were plated in a 20 uL suspension of 10% BME in ultra-low attachment 384 well plates (Corning). Twenty-four hours after plating ...
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bioRxiv - Developmental Biology 2023Quote: ... 70% EPM1 cells and transferred in a total of approximately 9000 cells to one well of an ultra-low-attachment 96-well plate (Corning). The protocols continued as described in “Generation of patterned Human organoids”.
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bioRxiv - Biochemistry 2023Quote: Spheroid cultures were generated by seeding a defined number of cells onto Nunclon Costar ultra-low attachment (ULA) round bottom 96 well plates (Corning) in 100 µL of cell culture medium ...
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bioRxiv - Biochemistry 2022Quote: ... 8*105 cells were seeded in 150 µL of FBS-free media per well in a 96-well ultra-low attachment microplate (#CLS3474, Corning) and allowed to settle for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested and dissociated into single-cell suspension using Accutase (#LM-T1735, Biosera) and seeded into ultra-low attachment 6-well plates (#CLS3471-24EA, Corning) at a density of 1000 cells/well in a defined serum-free medium ...
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bioRxiv - Cancer Biology 2023Quote: For spheroid growth assay 2 x 103 cells were seeded in a 96- well round-bottom ultra-low attachment plate (Corning) with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... For anoikis assay 1 x 103 cells were seeded in a 96-well flat-bottom ultra-low attachment plate (Corning) with 1% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% Penicillin/Streptomycin (v/v) for four additional days in 6 well ultra-low attachment plates (Corning, Cat#3473). Thereafter ...
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bioRxiv - Cell Biology 2023Quote: ... 200,000 cells/well were resuspended in E8 containing 10 μM Y27632 ROCK inhibitor (Selleckchem) in low adherence 6-well plates (Corning). For the first 10 days ...
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bioRxiv - Physiology 2023Quote: ... Enteroids were then centrifuged at 200g for 3 minutes and resuspended in WENR or EN media (WENR without Wnt and R-Spondin) and seeded in ultra-low-attachment 24-well plates (Corning Costar ...
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bioRxiv - Developmental Biology 2023Quote: ... the same protocol as above was followed with slight modifications: (i) all steps were performed in ultra-low attachment 24-well plates (Corning), (ii ...
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bioRxiv - Bioengineering 2023Quote: ... and 1 x 10 6 iLPCs were plated onto each well of an ultra-low attachment 6-well plate (Corning). LPC medium for this step was modified to increase the R-Spondin1 concentration to 10% ...
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bioRxiv - Bioengineering 2023Quote: ... was dropped onto each microwell platform for immunostaining and 5 μL of microgels (50 μL of microgel/cell solution) were added onto three wells of an ultra-low attachment round bottom 384-well plate (10185-096, Corning) for RT-qPCR ...
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bioRxiv - Immunology 2023Quote: ... 200 cells of each B cell subset were FACS sorted directly into 1× NEBNext Cell Lysis Buffer in 1.7 ml low binding tubes (Corning #3207). Cells were then flash frozen in liquid nitrogen for future use ...
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bioRxiv - Microbiology 2023Quote: ... assays were performed as above except that 104 A549 cells were grown in Costar Ultra-Low attachment 96-well plates (Corning) for 72 hours at which point spheroids had formed ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were seeded together with the virus in ultra-low attachment plates: for 250-5000 cells/sphere in Elplasia plates (Corning) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... hiPSCs were first dissociated into single cells and seeded in ultra-low attachment round-bottom 96-well plates (Corning, 7007), containing human embryonic stem cell (hESC ...
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bioRxiv - Cancer Biology 2023Quote: Melanoma cells transfected with TIPE or TIPE-sh were plated onto 24-well low attachment surface polystyrene plates (Corning, USA). Cells were grown in DMEM/F12 medium (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 120 µL of culture medium was removed from the ultra-low attachment U-bottom 96-well assay microplates (Corning, #7007) containing the organoids ...
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bioRxiv - Microbiology 2023Quote: ... was confirmed and EBs were transferred with wide-bore 200 uL tips to ultra-low attachment 24-well plates (Corning), previously filled with 500 μL/well of Induction Medium ...
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bioRxiv - Genomics 2023Quote: Spheroids were formed by seeding cryopreserved primary human hepatocytes (PHH) of a male donor in ultra-low attachment 96-well plates (Corning) as previously described [12] ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell types were plated at 100K cells in 200µl 50%/50% media in U-bottom 96-well ultra-low attachment plates (Corning, 3474). CD8+ T cells were stained with CellTrace Violet Proliferation Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 60-μl drops of the Matrigel cell cluster suspension were plated onto ultra-low attachment 96- or 48-well plates with a flat bottom (Corning) and allowed to solidify at 37 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were plated in 50 µL of media at 5,000 cells/well for NST and 15,000 cells/well for ILC cell lines in 2D (Fisher #353072) and Ultra-Low Attachment (ULA) (Corning #3474) 96-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 1,000 OV90 and 2,500 CAOV3 cells were transferred to Ultra low attachment U bottom black wall 96 well plates (Corning: 4515) in 200 ul medium ...
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bioRxiv - Cancer Biology 2023Quote: ... 1,500–4,000 cells containing either a Dox-inducible non-targeting control shRNA or EIF4EBP1-targeting specific shRNAs were seeded in Costar Ultra-low attachment plates (Corning) for 8–14 d in 150 µl growth medium ...
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bioRxiv - Developmental Biology 2023Quote: ... was diluted into 500Lµl Cortical Differentiation Medium IV (CDMIV, without Matrigel) in a 24-well ultra-low attachment plate (Corning, # 3473) containing a single organoid ...
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bioRxiv - Bioengineering 2023Quote: Day 9 metanephric mesenchyme-stage nephron progenitors were lifted with Accutase and re-plated at 50,000 cells per well in 96-well ultra low attachment round-bottom plates (Corning 7007) in Advanced RPMI 1640 Medium ...
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bioRxiv - Neuroscience 2023Quote: ... individual organoids were transferred to a single well of an ultra-low attachment U-bottom 96-well plate (Corning, #7007) after 25 to 26 weeks of maturation ...
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bioRxiv - Developmental Biology 2023Quote: 40 µl of N2B27 containing 300 ESCs were seeded into each well of a 96well ultra-low attachment U-bottom plate (Catalog no. 7007, Corning). After 48 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were seeded in a specific density of 1.5 x 105 cells/ml in ultra-low attachment dishes (Corning #3261) to allow embryoid body (EB ...
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bioRxiv - Neuroscience 2023Quote: ... and the embedded EBs were cultured in neural differentiation medium without Doxycycline in 6 well ultra-low binding plates (Corning). In this step ...
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bioRxiv - Bioengineering 2024Quote: Dissociated E17 cells were plated at 50,000 cells per well in 96-well ultra low attachment round-bottom plates (Corning 7007) in Advanced RPMI 1640 Medium and Glutamax (see Primary mouse embryonic kidney dissociation ...
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bioRxiv - Biochemistry 2024Quote: ... 8*105 cells were seeded in 180 µL of FBS-free media per well in a 96-well ultra-low attachment microplate (#CLS3474, Corning) and allowed to settle for 2 hours ...
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bioRxiv - Genomics 2024Quote: ... We re-suspended 900,000 cells in 15ml of StemFlex with 50μM Y-27632 and seeded the cells across a 96-well ultra-low attachment plate (Corning CLS7007) to form embryoid bodies ...
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bioRxiv - Immunology 2024Quote: ... Macrophages and target cells were cocultured at a 5:1 E:T ratio for 3 hours at 37 °C in ultra-low attachment 96-well plates (Corning 7007), unless otherwise indicated ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×104 labeled tumor cells were mixed with varying ratios of NK-92 cells or primary NK cells in 200 µL of growth media of the tumor cell line in the absence of IL-2 and co-cultured in an ultra-low attachment U-bottom 96-well plate (Corning) for 4 hours at 37°C in 5% CO2 ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... Various devices were molded from the masters using Sylgard™ 184 PDMS (Dow Corning). The base and crosslinker were mixed at a 10:1 mass ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... in a 1:1 mix of plain growth media and Matrigel (Corning). After approximately one week post transplantation when the tumors were palpable ...
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bioRxiv - Biophysics 2021Quote: ... The aggregations were performed in a 96-well half area clear flat bottom polystyrene NBS (low bind) micro-plate (3881, Corning, US) while shaking at 500 RPM and 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then suspended with trypsin and counted prior to plating approximately 5,000 cells per well into a 96-well ultra-low attachment surface plate (Corning, cat# 4515). Pre-plating the cells appeared to improve viability and spheroid formation compared to directly sorting into the ultra-low attachment surface plate ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells (1×10^6 or 1×10^5/well) were put onto ultra low attachment 24 well or 96 well plate (Corning, Inc) to allow them to form aggregated in suspension with ROCK inhibitor (2-5 μmol) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning, #CLS7007). On the following day ...
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bioRxiv - Bioengineering 2022Quote: ... organoids were retrieved with a pipette and cultured individually until day 16 in an ultra-low attachment 24-well plate (Corning #CLS3473). Control organoids were not induced ...
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bioRxiv - Molecular Biology 2021Quote: ... washed twice with LIF-free media and seeded as a single cell suspension on ultra-low attachment T75 flasks (Corning, 3814) at 2×106 cells per flask ...
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bioRxiv - Bioengineering 2022Quote: ... HyClone) with low glucose (high glucose for Colo-357 and PANC-1) supplemented with 10 % fetal bovine serum (FBS, Corning, USA), 1% Antibiotic-Antimycotic (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: MB-PDX or DAOY cells were cultured in NS culture media [50% DMEM + 50% F12 + 1× B27 supplement + 10 ng/ml human recombinant bFGF + 10 ng/ml EGF + 1× AAS] or adherent culture media (DMEM+10% FBS + 1× AAS) in ultra-low attachment (Corning, 3814) or tissue culture treated (Corning ...
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bioRxiv - Biochemistry 2019Quote: ... Kinase reactions were started by adding 2 µL of this substrate stock in a 384-well black low volume plates (Corning 4514). Proteins were thawed on ice and spun down to remove precipitate ...
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bioRxiv - Biochemistry 2019Quote: ... pellets were resuspended in 25 μl HNC and added to a black opaque low-adsorption 96-well assay plate (Corning 3991) containing 3 μl 10% Triton X-100 ...
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bioRxiv - Bioengineering 2020Quote: ... Gel droplets containing organoids were gelled at 37°C for 15 min before being transferred back into NIM in ultra-low adherence 6-well plates (Corning, #3471), which were held stationary ...