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Citations for Jackson ImmunoResearch :
151 - 200 of 304 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cryosections were washed 3 times and incubation with the secondary antibody Cy3-anti-rabbit (1:250, Jackson ImmunoResearch) and counterstaining with WGA was performed for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Detection was done with either HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Jackson ImmunoResearch cat#211-032-171, 111-035-144), followed by enhanced chemiluminescence (EZ-ECL ...
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bioRxiv - Physiology 2020Quote: ... indirect immunohistochemical detection was carried out using Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) conjugated to Horseradish Peroxidase (Jackson ImmunoResearch, West Grove, PA) diluted 1:1000 in 2% NGS/PBST ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked with 0.22 µm-filtered 5% Donkey Serum for 1 h at RT before staining for 1h with AF647 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, #711-606-152). After four washes ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X with TBS containing 0.1% Tween-20 and incubated for 1 hr RT with secondary antibody conjugated to horseradish peroxidase diluted into blocking buffer (Jackson ImmunoResearch—West Grove, PA). Blots were washed a final 3X and imaged using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were washed in PBS + 0.3% triton X-100 for 2 h at RT then incubated in cross-adsorbed Alexa-594 anti-rat and Alexa-488 anti-mouse secondary antibodies (Jackson ImmunoResearch, both 1:400) for 48 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3x with PBS and incubated for 1 h at RT in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in a blocking buffer containing 10% normal donkey serum (NDS) (cat. 017-000-001, Jackson ImmunoResearch, West Grove, USA), 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...
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bioRxiv - Cell Biology 2023Quote: ... the blot was washed 3 times with 1× TBST for 15 min each at RT and then incubated with horseradish peroxidase–conjugated goat anti-mouse IgG antibody (1:20000, Jackson ImmunoResearch 115-035-003) diluted in 5% non-fat milk for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3 times for 10 mins with TBS-Tween buffer and incubated for 1 h at RT with anti-rabbit (HRP) secondary antibody (1:20000, Ca# 711-035-152; Jackson ImmunoResearch Lab, Pennsylvania, USA) for chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were rinsed three times in 1X wash buffer for 2m at RT before adding secondary antibody (goat anti-chicken Alexa 647, 1:250 in PBS with 1% BSA; Jackson Immunoresearch #103-605-155). Slides were rinsed five times in 1X wash buffer for 2m at RT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed in 0.1% saponin and 1% BSA in PBS three times and incubated with 0.25% fluorescent secondary antibodies (Jackson ImmunoResearch) or 0.2% avidin conjugated with FITC (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed three times in PBS and incubated with appropriate Cy3- or FITC-conjugated secondary antibodies (Jackson ImmunoResearch) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... in PBS at 3 times and were incubated with Cy3-conjugated mouse anti-rabbit IgG (1:2,000; Jackson ImmunoResearch) in 0.5% BSA in PBS for 30 min at 37°C as a secondary antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... then were washed three times with PBS and incubated with blocking solution of 10% normal donkey serum (Jackson immunoResearch) in PBS with 0.2% Tween20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed three times for 10 min and incubated with horseradish peroxidase (HRP)-coupled secondary antibodies (Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed three times in PBS and incubated with anti-rabbit secondary antibody (1:500; Jackson ImmunoResearch) in PBS at room temperature for 2-4 h ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in PB 0,12 M and incubated with normal rabbit serum (1:20) (Jackson Immunoresearch) diluted in PB 0,12 M for 2H at RT ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBST and incubated in block with secondary antibody (Jackson ImmunoResearch # 711-625-152) and Hoechst (Thermo Scientific #62249 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times with PBS and incubated with the appropriate secondary antibodies (Invitrogen and Jackson Immunoresearch Laboratories), diluted 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed six times with PBS- T before the addition of anti-mouse IgG-HRP (Jackson ImmunoResearch Laboratories) and then incubated at 37°C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... Plates were then washed three times with PBS and secondary cross-adsorbed anti-human Fab-HRP (Jackson ImmunoResearch Laboratories) detection antibody was added at 1:10000 dilution in 5% NFDM-PBS for 1 hr at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed three times with PBS and incubated with fluorophore-labeled appropriate secondary antibodies purchased from Jackson Immunoresearch. Cells were analyzed on an Olympus fluorescence microscope and images were acquired with a Zeiss Axiocam camera.
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bioRxiv - Animal Behavior and Cognition 2024Quote: The sections were washed three times in a PBS buffer and incubated in blocking solution [5% NDS (Jackson ImmunoREsearch)/0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... and secondary antibodies diluted in blocking buffer were applied for 1 hour at RT (TRITC for α-SMA; Cy5 for fibronectin, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed in PBS 3X10 min at RT and then incubated with the appropriate secondary antibody (Cy5, 1:500; Rhod, 1:250; Jackson ImmunoResearch Laboratories, West Grove, PA) at RT for 2h in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and incubated for 1 hour at RT in biotinylated donkey anti-rabbit antibody (1:250, 711-065-152, Jackson Immunoresearch Laboratories, West Grove, PA) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Immunology 2023Quote: ... followed by detection with an AlexaFluor 647-conjugated anti-rabbit or anti-mouse IgG secondary antibody (Jackson Immunoresearch, cat# 111-605-046, cat# 115-605-174) -1:800 ...
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bioRxiv - Neuroscience 2019Quote: ... brain tissue sections were washed with six 5-minute PBS exchanges and then blocked for 2 hours at room temperature (RT, 20–21°C) with 3% normal donkey serum (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) dissolved in PBS-azide containing 0.25% Triton X-100 (PBT-azide) ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in blocking buffer containing 10% normal donkey serum (NDS) (Jackson ImmunoResearch, West Grove, USA, #017-000-001), 1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed 3x with PBS and incubated in secondary antibody for 2h at RT (Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 711-165-152 1:500). Slides were then washed 3x with PBS and mounted with Prolong antifade DAPI medium (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times for 10 minutes in TBST and incubated in horseradish peroxidase-conjugated secondary antibodies (1:10,000; Jackson ImmunoResearch) at room temperature in TBST with 5% milk for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The plates were washed three times before addition of alkaline phosphatase-conjugated Goat anti-human IgG (Jackson ImmunoResearch, Cambridgeshire, UK) diluted 1:1000 in 1 % milk in PBS with 0.05 % tween ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS (3 times) and stained with Cy3-conjugated donkey anti-rat IgG (Jackson ImmunoResearch Laboratories; 1.25 µg/ml). To detect total channels ...
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bioRxiv - Neuroscience 2020Quote: ... The brain sections were washed three times with PBS and incubated with the appropriate Cy3-conjugated secondary antibodies (Jackson ImmunoResearch) for 2 hours at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times for 10 minutes with TBST and horseradish peroxidase-conjugated anti-rabbit secondary antibody (Jackson immunoresearch) was incubated for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... Both membrane pieces were then separately washed with TBST 3 times for 20 min before incubation with secondary antibodies (245-80 kDa: goat anti-rabbit IgG, 1:10,000, Jackson ImmunoResearch, cat# 111-035-144 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then washed multiple times in PBS and incubated with Alexa488-Streptavidin (1:200; #016-540-084, Jackson ImmunoResearch) or AlexaCy5-Streptavidin (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed three times and a 1:3000 dilution of HRP-labeled donkey anti-human IgG (Jackson Immunoresearch) in Casein was added for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed three times with PBS-T then incubated with peroxidase-conjugated Goat anti-Human IgG (Jackson ImmunoResearch) diluted in blocking buffer for 1h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... The slides were washed 3 times with 1X PBS followed by incubation with fluorescent dye-conjugated secondary antibodies (Jackson ImmunoResearch) at room temperature for 1hr ...
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bioRxiv - Neuroscience 2023Quote: ... then washed three times in PBT and incubated in secondary antibody mix (goat anti-chicken Alexa Fluor 488, Jackson Immunoresearch #103-545-155 ...
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bioRxiv - Molecular Biology 2024Quote: ... brains were left rocking at 4°C for 24-72 hours (see list of dilutions and incubation times below) in 0.1% PBS-T supplemented with normal goat serum (Jackson ImmunoResearch) at a 1:20 dilution ...
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bioRxiv - Neuroscience 2021Quote: The brain sections undergoing immunohistochemical staining were washed in PBS 3 times (10 minutes each time) and incubated in a blocking solution containing 0.3% TritonX-100 and 5% normal donkey serum (Jackson ImmunoResearch, USA) in PBS for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rinsed three times in 1X PBS and incubated in secondary antibody solution Cy-3 donkey anti-rabbit (1:500; Jackson ImmunoResearch) or Cy-3 donkey anti-mouse (1:500 ...
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Peripheral CB1 receptor blockade acts as a memory enhancer through an adrenergic-dependent mechanismbioRxiv - Neuroscience 2021Quote: ... slices were rinsed 3 times in PB and incubated at room temperature with the secondary antibody AlexaFluor-555 donkey anti-rabbit (1:1000, Jackson ImmunoResearch) in DS-T-PB for 2 h ...