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Citations for Jackson ImmunoResearch :
51 - 97 of 97 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and bound antibodies were detected and captured with anti-mouse IgG secondary antibodies (1:300 dilution, RT, 1 h) (Jackson ImmunoResearch). The brain sections were imaged and analyzed with a Zeiss LSM 780 confocal microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR, Jackson ImmunoResearch 115-165-003 ...
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Nociceptor neuroimmune interactomes reveal cell type- and injury-specific inflammatory pain pathwaysbioRxiv - Neuroscience 2023Quote: ... Slides are then washed 3x in PBS and incubated in secondary antibody at RT for 2h (Donkey anti-Rb Cy3 Jackson ImmunoResearch 711-165-152 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated 1 hr at RT with Peroxidase-AffiniPure Goat Anti-Mouse (1:5,000; Jackson ImmunoResearch, Cat# 115-035-003) or Anti-rabbit IgG ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1h at room temperature (RT) or overnight at 4 °C in 5% normal donkey serum (NDS) in PBS-TX (Jackson Immunoresearch). Primary antibodies were chicken anti-GFP (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... incubated for 1h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch) or anti-rabbit (111-035-003 ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated for 1 h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch). After 3 washes ...
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bioRxiv - Immunology 2023Quote: ... incubated for 1h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch) or anti-rabbit (111-035-003 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times with PBS and incubated with the respective secondary antibody at 1:500 in 3% donkey serum with 0.1% Triton X-100 in PBS for 2h at RT (anti-rabbit Alexa Fluor 568 and anti-mouse Alexa 488, both from donkey, Jackson Immunoresearch). Cells were washed once and incubated with DAPI (40,6-diamidino-2-phe-nylindole ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed with PBS in a coplin jar for 30 min at RT then incubated with donkey secondary antibodies (Jackson ImmunoResearch) diluted in PBST at RT for 1 hr ...
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bioRxiv - Evolutionary Biology 2023Quote: ... peroxide blocked for 30m (RT) then incubated with biotinylated goat anti-rabbit secondary antibody (Jackson ImmunoResearch 111-065-144, 1:5000) for 1h (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... Then after several washes the sections were incubated in a cocktail of the following secondary antibodies for 2 h at RT: 1:200 donkey anti-chicken Alexa Fluor 488 (703-545-155, Jackson ImmunoResearch), 1:500 donkey anti-goat Alexa Fluor 594 (A21207 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hour at RT with the biotinilated secondary antibody: donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 1:200), followed by an avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Cell Biology 2022Quote: ... followed by a 2h incubation at RT with the secondary antibody donkey anti-rabbit Alexa Fluor® 647-conjugated Affinipure (Jackson Immunoresearch). Nuclei were counterstained with DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were permeabilized and blocked with 3% BSA containing 0.1% Triton X100 in PBS for 20 mins at RT and labelled with anti-mouse Cy3 antibody (Jackson ImmunoResearch, 1:400) for 1 hr at RT ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed 3 times in PB and incubated first in 10% NGS (15 min) and then in a solution containing anti-rabbit biotinylated secondary antibodies (1:500; 1.5 hr at RT; Jackson ImmunoResearch Europe Inc.). Sections were subsequently rinsed in PB ...
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bioRxiv - Developmental Biology 2019Quote: ... blots were incubated for 1-2 hours at RT with 1:20,000 donkey anti-mouse horseradish peroxidase (Jackson ImmunoResearch #715-035-150). Immunoblots were developed using SuperSignalTM West Pico/Femto Sensitivity substrate (Thermo Scientific #34080 ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were washed with PBS + 0.1% Triton X-100 and incubated 60 min at RT with secondary antibodies (Jackson ImmunoResearch, Thermo Fisher) diluted 1:1000 in PBS + 0.1% Triton X-100 ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated with anti-guinea pig Cy5 and anti-rabbit-Cy3-conjugated antibodies for 2.5 hours at RT (1:200, Jackson ImmunoResearch Laboratories Inc.) followed by extensive rinsing in PB ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed with 0.1% Tween/TBS and incubated at RT for 1 h with horseradish peroxidase conjugated secondary antibodies (Jackson Immunoresearch, 1:10,000) in 5 % milk ...
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bioRxiv - Zoology 2020Quote: ... Buds were incubated at RT for 1 h with Alexa Fluor-coupled secondary antibodies (1:500 in PBS) (anti-mouse or anti-rabbit (Jackson ImmunoResearch or LifeTechnologies)) ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and incubated for 2 h at RT with donkey anti-mouse Cy3 or donkey anti-rabbit Alexa 488 secondary antibodies (Jackson Immunoresearch, Suffolk, UK). Finally ...
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bioRxiv - Immunology 2024Quote: ... washed and overlaid with HRP-conjugated goat anti-mouse secondary antibodies diluted 1:5,000 in blocking solution for 1h at RT (Jackson ImmunoResearch, #115-035-003) and subsequently washed ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked with 0.22 µm-filtered 5% Donkey Serum for 1 h at RT before staining for 1h with AF647 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, #711-606-152). After four washes ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X with TBS containing 0.1% Tween-20 and incubated for 1 hr RT with secondary antibody conjugated to horseradish peroxidase diluted into blocking buffer (Jackson ImmunoResearch—West Grove, PA). Blots were washed a final 3X and imaged using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were washed in PBS + 0.3% triton X-100 for 2 h at RT then incubated in cross-adsorbed Alexa-594 anti-rat and Alexa-488 anti-mouse secondary antibodies (Jackson ImmunoResearch, both 1:400) for 48 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3x with PBS and incubated for 1 h at RT in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in a blocking buffer containing 10% normal donkey serum (NDS) (cat. 017-000-001, Jackson ImmunoResearch, West Grove, USA), 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...
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bioRxiv - Cell Biology 2023Quote: ... the blot was washed 3 times with 1× TBST for 15 min each at RT and then incubated with horseradish peroxidase–conjugated goat anti-mouse IgG antibody (1:20000, Jackson ImmunoResearch 115-035-003) diluted in 5% non-fat milk for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3 times for 10 mins with TBS-Tween buffer and incubated for 1 h at RT with anti-rabbit (HRP) secondary antibody (1:20000, Ca# 711-035-152; Jackson ImmunoResearch Lab, Pennsylvania, USA) for chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were rinsed three times in 1X wash buffer for 2m at RT before adding secondary antibody (goat anti-chicken Alexa 647, 1:250 in PBS with 1% BSA; Jackson Immunoresearch #103-605-155). Slides were rinsed five times in 1X wash buffer for 2m at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... and secondary antibodies diluted in blocking buffer were applied for 1 hour at RT (TRITC for α-SMA; Cy5 for fibronectin, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed in PBS 3X10 min at RT and then incubated with the appropriate secondary antibody (Cy5, 1:500; Rhod, 1:250; Jackson ImmunoResearch Laboratories, West Grove, PA) at RT for 2h in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and incubated for 1 hour at RT in biotinylated donkey anti-rabbit antibody (1:250, 711-065-152, Jackson Immunoresearch Laboratories, West Grove, PA) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... brain tissue sections were washed with six 5-minute PBS exchanges and then blocked for 2 hours at room temperature (RT, 20–21°C) with 3% normal donkey serum (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) dissolved in PBS-azide containing 0.25% Triton X-100 (PBT-azide) ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in blocking buffer containing 10% normal donkey serum (NDS) (Jackson ImmunoResearch, West Grove, USA, #017-000-001), 1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed 3x with PBS and incubated in secondary antibody for 2h at RT (Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 711-165-152 1:500). Slides were then washed 3x with PBS and mounted with Prolong antifade DAPI medium (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Membranes were washed with 0,1% Tween-20 in PBS (5 times) and incubated for 1 hour at room temperature (RT) with horseradish peroxidase-conjugated secondary antibodies (1:2000, Jackson ImmunoResearch, 515-035-003 and 711-035-152). Membranes were washed again before protein detection ...