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Citations for Jackson ImmunoResearch :
151 - 200 of 215 citations for SFT2D3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Tissue was then rinsed in TBS and incubated for 1 h in the blocking solution containing the secondary antibody Alexa 488 anti-goat raised in donkey (1:500; 705-545-147, Jackson ImmunoResearch). Following rinses in TBS ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then rinsed three times with PBS and permeabilized with blocking buffer (3% bovine serum albumin (BSA, 001-000-162, Jackson ImmunoResearch) and 0.2% Triton X-100 (X100 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed in 0.1M PBS and 0.1% TritonX-100 in PBS before incubation with blocking solution (5% donkey serum (Jackson ImmunoResearch, Pennsylvania, US) in 0.05% Triton-X100 in 0.1M PBS) ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (catalog no. 005–000–121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (catalog no ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 3 h in the blocking solution with the addition of 16 µg/ml of donkey anti-guinea pig Alexa Fluor 647 (Jackson ImmunoResearch). Following two more 30-min PBS rinses ...
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bioRxiv - Biophysics 2021Quote: ... Non-specific staining was limited through a blocking step using 10% (w/v) Bovine Serum Albumin (BSA) (Jackson ImmunoResearch, PA, USA) for 2 hours at room temperature (measured at 22-23°) ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated for 1-2 hrat room temperature with blocking solution of 5% goat serum in PBS (005–000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GTX135357 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed again 3 times for 5 minutes each in 0.1M PBS before being incubated in blocking buffer with 1:1000 Alexa Fluor® 594 Streptavidin (Jackson Immunoresearch) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The brain slices were subsequently incubated in blocking buffer (5% donkey serum in TBS-T) containing appropriate fluorescence-conjugated secondary antibodies (1:1000; Jackson ImmunoResearch; Dylight 405-conjugated donkey anti-guinea pig IgG [706-475-148] ...
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bioRxiv - Bioengineering 2022Quote: ... Fixed samples were permeabilized and blocked overnight at 4 °C with a blocking buffer containing 5% IgG-free bovine serum albumin (BSA) (Jackson ImmunoResearch) and 0.3% Triton X-100 (Bio Basic ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.3% PBT were followed by 30 min incubation in blocking solution with 5% NGS (005-000-121, Jackson Immunoresearch Laboratories) in PBT ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed three times for 10 minutes in 0.1M Tris-buffered saline (TBS) and then incubated in 0.1M TBS with 5% blocking buffer (Normal Donkey Serum, Jackson Immunoresearch, NC9624464) and 0.3% Triton-X 100 for 2 hours at room temperature ...
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bioRxiv - Physiology 2023Quote: ... samples were incubated with secondary antibodies diluted 1:500 and DAPI (1:1000) in blocking/permeabilisation buffer (Dk-a-Ch-AF488 – 703-545-155 Jackson Immunoresearch, Dk-a-Rb-AF546 – A10040 Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... then blocked for 45 min before 2 h incubation at room temperature in Alexa Fluor 488-conjugated donkey anti-chicken IgG (1:400 in blocking solution, Jackson ImmunoResearch). Sections were rinsed in PBS after the final incubation and mounted with Vectashield Hardset Mounting Medium with DAPI (Vector Labs) ...
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bioRxiv - Biochemistry 2023Quote: ... Triton X-100 in PBS at room temperature for 15 min and washed with the blocking buffer [5% (v/v) normal goat serum (NGS, Jackson ImmunoResearch) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... sections were blocked for 1 hour at room temperature in blocking buffer: 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121) in PBST (0.1% TritonX-100 in PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... floating sections were blocked for 1 hour at room temperature in blocking solution (3% normal goat serum (NGS, Jackson ImmunoResearch Laboratories), 0.3% Triton X-100 (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... All sections were rinsed three times for 15 min in PBS-T and incubated for 4 hours at ∼20 °C in secondary antibodies in the blocking solution (Cy3 donkey anti-guinea pig, 1:400, Jackson ImmunoResearch, catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed in PBS and then blocked for 1 hour in Blocking Buffer consisting of 10% normal donkey serum (017-000-121, Jackson ImmunoResearch), 0.5% Triton X-100 (T8787 ...
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bioRxiv - Neuroscience 2021Quote: ... After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature: 1:500 dilution of Donkey anti-rat Alexa Fluor 488 (Jackson ImmunoResearch Laboratories), 1:200 dilution of Donkey anti-guinea pig Alexa Fluor 647 (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then transferred to fresh blocking buffer with diluted antibodies (RyR2 1:500, NCX 1:200, SERCA 1:2000, GAPDH 1:5000) (Jackson ImmunoResearch, PA) followed by overnight incubation on a shaker at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in PBS and were incubated in blocking solution [PBS supplemented with 3% donkey serum (Jackson ImmunoResearch, 017-000-121) and 0.1% Triton-X] for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with PBS and incubated with Donkey anti-Chicken secondary antibody conjugated with alexa fluor 488 (1:500 in blocking solution, 703-545-155 Jackson ImmunoResearch laboratories).After three final washes with PBS ...
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bioRxiv - Microbiology 2021Quote: ... A blocking solution (10% normal donkey (#017-000-121) or normal goat serum (#005-000-121, Jackson ImmunoResearch Labs, West Grove, PA) diluted in PBS with 0.2% Tween-20 ...
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were washed with PBS twice for 10 min followed by blocking for 1 h in a dark humid chamber with 10% normal donkey serum (#017-000-121, Jackson ImmunoResearch, USA) diluted in PBS ...
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bioRxiv - Genomics 2023Quote: ... Cells were permeabilized for 5min with PBT (PBS with 0.5% Triton) and then incubated with blocking buffer (10% normal donkey serum (Jackson ImmunoResearch, Cat# AB2337258) in PBT ...
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bioRxiv - Cell Biology 2022Quote: ... and a subsequent permeabilization and blocking step with PBS containing 0.1% saponin and 1% IgG-free/protease free bovine serum albumin (BSA) (Jackson ImmunoResearch 001-000-161) for 1 h at RT ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 3 times 10 min in 0.1% Triton X-100 in PBS at room temperature and then incubated for 2h with secondary antibodies diluted in blocking buffer: donkey anti-rabbit-IgG-Cy3 antibody (1:1000, Jackson ImmunoResearch, RRID:, AB_2307443), donkey anti-mouse-IgG-Cy5 antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilization and blocking of endogenous proteins were done with 0.5% Triton X-100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017-000-121) in PBS for 30 min ...
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bioRxiv - Immunology 2024Quote: ... washed and overlaid with HRP-conjugated goat anti-mouse secondary antibodies diluted 1:5,000 in blocking solution for 1h at RT (Jackson ImmunoResearch, #115-035-003) and subsequently washed ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed and placed into Alexa488-conjugated donkey anti-rabbit immunoglobulin G (IgG; 1:500 in blocking buffer; Jackson Immunoresearch, West Grove, PA) for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 45-60 min in blocking solution that consisted of PBS containing 3% Bovine Serum Albumin (Jackson Immunoresearch, 001-000-162) and 3% Donkey Serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X with TBS containing 0.1% Tween-20 and incubated for 1 hr RT with secondary antibody conjugated to horseradish peroxidase diluted into blocking buffer (Jackson ImmunoResearch—West Grove, PA). Blots were washed a final 3X and imaged using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... The chips were then washed twice with PBS, and the cells were permeabilized with blocking solution (PBS 0.1% Tryton-X, 3% of Donkey Serum (Jackson Immunoresearch Cat#017-000-121)) for 45 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... for 20min at room temperature and then incubated in a blocking solution made of PBST with 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were washed and placed into Alexa 488-conjugated donkey anti-rabbit immunoglobulin G (IgG; 1:500 in blocking solution; Jackson Immunoresearch, West Grove, PA) for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were first washed in PBS 5mins for 3 times and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in a blocking buffer containing 10% normal donkey serum (NDS) (cat. 017-000-001, Jackson ImmunoResearch, West Grove, USA), 1% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed once with PBS and blocked for one hour at room temperature in blocking buffer of PBS with 1% Normal Donkey Serum (Jackson ImmunoResearch #017-000-121), 0.1% Gelatin ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue was dissected to isolate the cochlea and vestibular organs and these were permeabilized for 30 minutes in blocking solution containing PBS with 1% bovine serum albumin (BSA) (Jackson ImmunoResearch 000-001-162) and 5% donkey serum (Jackson ImmunoResearch 017-000-121) ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: P5-6 retinas were incubated in wash buffer (PBS with 0.1%Triton X100) with gentle rocking for 15 min and then in blocking buffer (wash buffer with 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121)) for 1-2 hrs ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Cancer Biology 2020Quote: ... and secondary antibodies diluted in blocking buffer were applied for 1 hour at RT (TRITC for α-SMA; Cy5 for fibronectin, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times with diluted blocking buffer (15 min each) before incubation with secondary antibodies (Ch-647: Jackson Immunoresearch, Cat# 703-605-155) for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...