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Citations for Jackson ImmunoResearch :
1 - 50 of 3887 citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
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bioRxiv - Immunology 2024Quote: ... pro-IL-1β was detected using goat-α-IL-1β (R&D, #AB-401-NA, 1:1500) and bovine-α-goat-HRP (Jackson ImmunoResearch, #805-035-180, 1:10,000) and actin was detected using mouse-α-actin-HRP (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... incubated 2 h in blocking solution containing rabbit-anti-human amyloid beta (IBL America, #18584; 1:500) and donkey-anti-human AF647 (Jackson ImmunoResearch, 709-606-149; 1:250), washed 3x for 15 min each in PBS with 0.3% triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation with 1 µg/ml of recombinant sE WT or dimer-mutants or anti-human IgG-HRP (Jackson ImmunoResearch, 109-035-088, 1:5000) in PBS+0.5% FCS for 2 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocked samples were then incubated at 4°C overnight in primary antibody (rabbit anti-beta-galactosidase, Jackson ImmunoResearch Laboratories) dissolved at a 1:200 dilution in 1% BSA in PBS/0.1% Triton-X 100 ...
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bioRxiv - Bioengineering 2023Quote: ... an ELISA assay was developed to detect LH01 or un-cleaved LH05 with IL-15Rβ coated on the plate and Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) as the detection antibody ...
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bioRxiv - Immunology 2024Quote: ... 1 ng/ml mouse IL-6 and 10 µg/ml FITC-F(ab’)2 Goat anti-mouse IgG (Jackson Immunoresearch, USA, # 115-096-071). The cell mixture was plated in Omnitray dish (Nunc ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM MgCl2 and 0.1% Tween-20) with equimolar amounts of control Fc protein (Jackson ImmunoResearch), purified Tenascin-R-Fc or Brevican-Fc and rotated end-over-end for 1 hour at RT ...
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bioRxiv - Physiology 2022Quote: ... and a protein block of 10% goat serum with 1% bovine serum albumin (BSA; Jackson ImmunoResearch) was the applied for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated proteins were visualized with α-Streptavidin-HRP antibody (Jackson ImmunoResearch, 1:2000. #016-030-084). For mass spectrometry ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The resultant molding nanosurface is then cut in 1×1 cm squares by diamond pencil scribbling (on the reverse side of nano-surface) and precoated with biotinylated and fluorescent tag-labeled anti-Fc Fab antibody fragment (Jackson Immunoresearch; 0.1 mg/mL PBS solution ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was washed with TBT-Tween 0.1% and immunoreactive proteins were detected using anti-mouse IgG conjugated with peroxidase (1:10,000, Jackson ImmunoResearch) for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... ELISA of both sera and recombinant human antibodies was applied with AP-conjugated Donkey anti-human IgG (Jackson ImmunoResearch, USA ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were detected using HRP-coupled secondary antibody (1:15,000 goat anti-rabbit, Jackson ImmunoResearch Labs 111-035-144, RRID:AB_2307391). Chemiluminescence was detected using Amersham ECL reagent (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... The protein fraction was precleared for 1 h with 50 μL of normal rabbit serum (Jackson ImmunoResearch, 011-000-120) and 50 μL of agarose A/G bead slurry (Pierce™ Protein A/G Agarose ...
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bioRxiv - Immunology 2020Quote: ... ELISA of both sera and recombinant scFv-Fc human antibodies applied with AP-conjugated anti-human IgG (Jackson ImmunoResearch, USA) following detection using PNPP substrate (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Immunoreactive proteins were detected using a horseradish-peroxidase-labeled secondary antibody for mouse IgG (1:10,000; Jackson ImmunoResearch, 115-035-003) or for anti-rabbit IgG (1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were detected using HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories).
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then detected with horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch) and ECL chemiluminescent substrate (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... Protein signals were visualized by an HRP-conjugated secondary antibody (Jackson ImmunoResearch) and western HRP substrate (Sangon).
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were detected using appropriate HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories). All western blots are representative examples and have been repeated for at least two biological replicates.
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bioRxiv - Molecular Biology 2023Quote: ... ACC protein was detected using horseradish perodixase-conjungated streptavidin (Jackson ImmunoResearch, Cambridgeshire, UK).
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bioRxiv - Molecular Biology 2023Quote: ... The protein bands were detected with horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch) and Supersignal West Pico enhanced chemiluminescent solution (Thermo).
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bioRxiv - Molecular Biology 2023Quote: ... The protein bands were detected with horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch) and Supersignal West Pico enhanced chemiluminescent solution (Thermo).
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bioRxiv - Cancer Biology 2020Quote: ... To fabricate ICAM1 elastic nanotectures the Fab anti-Fc antibody fragment protein (Jackson Immunoresearch, USA) was prelabeled with a fluorescent tag and a biotin group to ensure both its fluorescent visibility in nanopatterns and cross-linking to the streptavidin-functionalized PAA gels ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was detected by incubation with HRP-conjugated sheep anti-mouse secondary antibody (Jackson ImmunoResearch) and blots were imaged on a Biorad Chemidoc Imaging System.
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bioRxiv - Microbiology 2023Quote: ... The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat#: 009-000-008) at 150 μg/mL for 200 s to occupy the free protein A ...
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bioRxiv - Microbiology 2023Quote: ... Antibody-bound proteins were recognized using horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Jackson ImmunoResearch). A chemiluminescent signal was detected using a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-rabbit 1:5000 (Jackson Immunoresearch 1:10000).
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bioRxiv - Neuroscience 2024Quote: ... or HRP (1:1000-1:5000, Jackson ImmunoResearch).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then transferred to fresh blocking buffer with diluted antibodies (RyR2 1:500, NCX 1:200, SERCA 1:2000, GAPDH 1:5000) (Jackson ImmunoResearch, PA) followed by overnight incubation on a shaker at 4°C ...
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bioRxiv - Immunology 2022Quote: ... goat anti-mouse:HRP (Jackson Immunoresearch, 1:5,000-1:30,000), mouse anti-rabbit:HRP (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... goat and guineapig (1/200-1/2000; Jackson ImmunoResearch). To enhance staining intensity or visualize sections in a conventional light microscope with DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... or AlexaFluor647 (Jackson ImmunoResearch Laboratory; 1:500-1:1000). 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Bioengineering 2023Quote: ... Fcγ Subclass 1 Specific (Jackson Immunoresearch Laboratories,1:100), Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG ...
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bioRxiv - Immunology 2022Quote: ... goat anti-mouse (Jackson Immunoresearch, 1:5000-1:10,000), mouse anti-rabbit (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... Fcγ Subclass 1 Specific (Jackson Immunoresearch Laboratories,1:100), Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG ...
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bioRxiv - Neuroscience 2020Quote: ... non-specific protein binding was blocked in a medium consisting of 2% normal donkey serum (Jackson ImmunoResearch) in antibody medium (AB medium ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with chemiluminescence using horser-adish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA). Before to immunoblot ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against the following proteins were used: Biotin (Bethyl A150-109 and Jackson Immunoresearch 200-002-211), ORC2 (Bethyl A302-734A) ...
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bioRxiv - Immunology 2021Quote: ... Reporter proteins were: Alexa647-labeled F(ab’)2 fragment of rabbit anti-human IgG Fc-specific (Jackson ImmunoResearch) used at 75nM final in-droplet concentration ...
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bioRxiv - Immunology 2021Quote: ... Bound Fc-tagged proteins were detected with anti-human HRP-conjugated secondary antibodies (Jackson ImmunoResearch – 109-035-088) at 1:3000 dilution in PBS 0.5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... Proteins of interest were detected with specific primary antibodies and secondary antibodies coupled to horseradish peroxidase (Jackson ImmunoResearch). Densitometry was measured with ImageJ (https://imagej.net).
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bioRxiv - Cell Biology 2023Quote: ... Fcγ Subclass 1 specific (1:2000, Jackson Immunoresearch #3115-585-205) and AlexaFluor® 488 conjugated goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... 1% normal donkey serum and 1% normal goat serum (Jackson ImmunoResearch Laboratories 017-000-121 and 005-000-121) ...
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bioRxiv - Genetics 2020Quote: ... Cy5 or Alexa 647 secondary antibody pre-absorbed against a range of mammalian serum proteins (Jackson Immunoresearch and ThermoFisher). This protocol was modified for FISH as described (Radford and McKim ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch), detected using SuperSignal West Pico or Femto chemiluminescence substrates (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... Protein-primary antibody complexes were revealed with horseradish peroxidase-labeled donkey anti-rabbit antibodies (Jackson ImmunoResearch, West Grove, PA) and ECL detection reagents (Amersham ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1 hour with biotin-conjugated secondary antibodies (1:500, Jackson Immunoresearch), for 1 hour with Vectastain Elite ABC amplification kit (Vector Laboratories ...