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Citations for Jackson ImmunoResearch :
51 - 100 of 586 citations for Recombinant Human TPBG Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 0.5% BSA and RBD-Fc binding was detected with goat-anti-human-Ig-APC (Jackson ImmunoResearch Laboratories, Pennsylvania, USA). Data was acquired using by flow cytometry using an LSRII (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-008) and the signal was developed as described above.
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bioRxiv - Immunology 2024Quote: Standard amine coupling (1:1 EDC:NHS) was used to covalently couple a goat anti-human Fc antibody (Jackson ImmunoResearch) to the HC30M chip ...
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bioRxiv - Immunology 2019Quote: ... and resuspended in stimulation buffer in the presence of 1μg/mL AlexaFluor 647 conjugated F(ab’)2 goat anti-human Fc antibody (Jackson ImmunoResearch) with or without 2 μg/mL ICAM-1 ...
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bioRxiv - Bioengineering 2023Quote: ... and 1:1000 dilution of goat anti-human Fc F(‘ab)2 Alexa Fluor 488 (Jackson ImmunoResearch, 109-546-170) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... A coupled lawn of goat anti-human IgG Fc (hFc; 50 µg/mL in 10 mM sodium acetate, pH 4.5) (Jackson ImmunoResearch) was then prepared before quenching with 0.5 M ethanolamine (pH 8.5 ...
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bioRxiv - Immunology 2021Quote: ... Fc-specific (Jackson ImmunoResearch).
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bioRxiv - Biophysics 2022Quote: ... Fc fragment (Jackson Immunoresearch) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... retroorbitally and serum was analyzed by Western blot for expression of FGFR1-ECD-Fc or Fc using anti-IgG2α Fc (Jackson ImmunoResearch). All adenoviral inserts were cloned into the E1 region of E1- E3- Ad strain 5 by homologous recombination and amplified in in 293 cells followed by CsCl2 gradient purification of virus ...
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bioRxiv - Immunology 2022Quote: ... the beads were washed three times in PBT and labelled for 30 min with R-Phycoerythrin-conjugated Goat-anti-Human IgG Fc (Jackson Immunoresearch, 30µl of a 1:600 dilution of stock in assay buffer) ...
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bioRxiv - Immunology 2019Quote: ... ICAM-1 was immobilized on maxisorp plates by first coating the plates overnight with 2 μg /mL anti-human Fc antibody (Jackson ImmunoResearch), washing 3X with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated with goat anti-human Fc fragment specific antibody for E-cadherin substrate (Jackson ImmunoResearch, West Grove, PA), at 1 μg / cm2 in 0.1 M pH 8 borate buffer at 4°C overnight in a humidity chamber ...
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bioRxiv - Microbiology 2021Quote: ... Membranes overlaid with rH3-Fc were subsequently probed with biotin-SP-conjugated AffiniPure goat anti-human IgG (Jackson ImmunoResearch; 1:2000). Immunodetection was performed using infrared dye-labeled secondary antibodies (IRDye 800CW Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and then incubated for 1 hr in blocking buffer without NaN3 to which of horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-human IgG Fc specific antibody (Jackson Immunoresearch) had been added at the dilution as recommended by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... a secondary antibody conjugated with alkaline phosphatase (AffiniPure goat anti-human IgG Fc fragment specific, Jackson ImmunoResearch Laboratories cat. #109-055-008) diluted 1:1000 in 1% BSA/PBS-T ...
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bioRxiv - Immunology 2021Quote: ... Fc fragment specific (Jackson ImmunoResearch) on ice for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-tagged secondary antibodies were from Jackson ImmunoResearch; fluorescent Alexa-tagged antibodies were from Molecular Probes.
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bioRxiv - Molecular Biology 2020Quote: ... HRP-tagged secondary antibodies were from Jackson ImmunoResearch; fluorescent Alexa-tagged antibodies were from Molecular Probes ...
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgG Fc-gamma (Jackson ImmunoResearch 109-135-098) to label for bound NHP antibodies or Alexa Fluor-647-conjugated goat anti-mouse-IgG Fc-gamma (Jackson ImmunoResearch 115-605-008 ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... which was detected by incubation with fluorescein isothiocyanate-tagged anti-mouse IgG antibody (green, 1:200) and rhodamine-tagged anti-mouse IgG antibody (red, 1:200) (Jackson ImmunoResearch, West Grove ...
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bioRxiv - Neuroscience 2020Quote: ... murine recPrP23-110 and human recPrP121-230 and BSA (coated at 43 nM) in ELISA using goat anti-human IgG Fc-gamma specific-HRP (Jackson ImmunoResearch #109-035-098), 1:4’000 for detection.
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bioRxiv - Immunology 2023Quote: ... containing human IgG1 constant regions were tested at 4µg/ml and at three four-fold dilutions and were detected with peroxidase-conjugated goat anti-human IgG Fc (Jackson ImmunoResearch, 109-035-098). Peroxidase was revealed with 1-Step ABTS substrate solution (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse-Fc-antibody (Jackson ImmunoResearch) was diluted in 10 mM sodium acetate buffer pH 5 (30 µg/mL ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse-Fc-antibody (Jackson ImmunoResearch) was diluted in 10 mM sodium acetate buffer pH 5 (30 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... secondary AlexaFluor-tagged antibodies raised in donkey (Jackson Immunoresearch) in 1%NDS/TBST were incubated for 4 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Horseradish peroxidase-tagged goat anti-mouse IgG (Jackson ImmunoResearch) was added at 1:1000 dilution to detect antigen bound IgG mAbs ...
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and the binding of VHH-Fcs to denatured S was probed by incubating strips with 1 mL of 100 ng/mL anti-human Ig Fc antibody-peroxidase conjugate (Jackson ImmunoResearch, Cat#016-030-084) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2023Quote: ... wells were washed 6 times and 1:5000 dilution of horseradish peroxidase (HRP)-conjugated donkey anti-human IgG (FC specific; Jackson ImmunoResearch Laboratories, West Grove, PA) was added in blocking solution to each well and the plate was incubated for 1 hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... followed by fluorescent-tagged secondary antibodies (1:500; Jackson Immunoresearch). Cells were co-stained with Rhodamine-phalloidin (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... Dishes were coated with 50 μg/ml Fc or GPC3coreUG protein mixed with 120 μg/ml anti-hFc (Jackson ImmunoResearch 109-005-098) for 30 min at 37°C and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation with 1 µg/ml of recombinant sE WT or dimer-mutants or anti-human IgG-HRP (Jackson ImmunoResearch, 109-035-088, 1:5000) in PBS+0.5% FCS for 2 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently with the according HRP-tagged secondary antibodies (Jackson Immunoresearch). Membranes were revealed with the ECL2 substrate according to the manufacturer’s instructions (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... followed by with fluorescent tagged secondary antibodies (Jackson Immunoresearch, 1:500) and DAPI (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... ANP32B (EPR14588) and subsequently with HRP-tagged secondary antibodies (Jackson Immunoresearch). Membranes were revealed with the ECL2 substrate according to the manufacturer’s instructions (Pierce ...
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bioRxiv - Immunology 2019Quote: ... The expression of GXMR-CAR on the surface of human T cells was analyzed via flow cytometry (MACSQuant; Miltenyi Biotech) using a goat anti-human IgG antibody (Fc-γ fragment-specific, 1:100 dilution; Jackson ImmunoResearch Laboratories; cat. no. 109-606-098). Human T cells were identified using flow cytometry with an anti-human CD3-PE antibody (1:100 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... Cy3-tagged goat anti-guinea pig antiserum (Jackson ImmunoResearch, West Grove, PA). All were used at a dilution of 1:1000.
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bioRxiv - Bioengineering 2022Quote: ... or 5-tagged secondary antibodies raised in donkey [1:500 (Jackson ImmunoResearch), at 22–24°C for 2 h] ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with fluorescent tagged secondary antibody (Jackson Immunoresearch, 1:500).
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bioRxiv - Neuroscience 2020Quote: ... and the Cy3-conjugated anti-Fc antibody (Jackson ImmunoResearch). Fluorophore-conjugated secondary antibodies were from Jackson ImmunoResearch or Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse Fc fragment (Jackson ImmunoResearch 015-010-008). FluoTag-XM-QC anti-mouse IgG kappa light chain (clone 1A23 ...
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bioRxiv - Cell Biology 2021Quote: ... derivatized as described previously,52 with anti-hu Fc mixed with equimolar anti-mu Fc antibodies (cat # 109-005-008 and 115-005-008, respectively; Jackson ImmunoResearch, West Grove, PA) at pH 5.0 ...
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bioRxiv - Cell Biology 2023Quote: ... FC 1:300 (all from Jackson ImmunoResearch Inc., Baltimore, USA). CellMask Orange Plasma membrane stain ...
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bioRxiv - Cell Biology 2023Quote: ... we first microprinted α-Fc FAB antibody fragment (Jackson Immunoresearch, USA ...
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bioRxiv - Cell Biology 2019Quote: ... All secondary antibodies were multi-labeling grade (tagged with Cy2, Cy3, Cy5, Jackson Immunoresearch). Following washes with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed and incubated in appropriate fluorescently tagged secondary antibodies (1:500; Jackson Immunoresearch) for 1 h followed by Hoescht staining (1:10,000 ...