Labshake search
Citations for Jackson ImmunoResearch :
601 - 650 of 674 citations for Recombinant Human CTLA4 His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
-
bioRxiv - Immunology 2020Quote: ... Slides were probed with a mixture of secondary antibodies consisting of Cy3-labeled goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:2,000 and Cy5-labeled goat anti-human IgM (Jackson ImmunoResearch ...
-
bioRxiv - Immunology 2020Quote: ... a secondary antibody conjugated with alkaline phosphatase (AffiniPure goat anti-human IgG Fc fragment specific, Jackson ImmunoResearch Laboratories cat. #109-055-008) diluted 1:1000 in 1% BSA/PBS-T ...
-
bioRxiv - Immunology 2022Quote: ... Proper CAR transduction was checked by flow cytometry by eGFP expression and by using AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch Laboratories).
-
bioRxiv - Immunology 2022Quote: ... The transfectant cells were incubated with supernatant containing expressed antibodies for 30 minutes then followed by incubation with APC-anti-human IgG (H+L) antibody (Jackson ImmunoResearch, USA). Bound antibodies to the GFP-positive cells were then analyzed by flow cytometry (Attune NxT ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by incubation with an HRP-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, 109-035-003; 1:1000 dilution in TBST) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... Bound antibodies were then detected using 1:2000 diluted alkaline phosphatase-labelled anti-human IgG Fcγ (Jackson ImmunoResearch Laboratories #109-055-098) and 4-Nitrophenyl phosphate disodium salt hexahydrate substrate (Sigma #S0942) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with Allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (1:100 dilution; catalog number 709-136-149; Jackson ImmunoResearch Laboratories) for 30 minutes ...
-
bioRxiv - Immunology 2023Quote: ... and then incubated with a 1:2500 dilution of horseradish peroxidase-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, West Grove, PA) in blocking buffer for one hour ...
-
bioRxiv - Pathology 2024Quote: ... the mAb reactivity was detected by mean of a Cy3-conjugated goat anti-human IgG serum (Jackson Immunoresearch Laboratories, West Grove, PA), diluted 1:1000 in 0.1% BSA in HBS ...
-
bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected with 50 μl of 1 μg/ml alkaline phosphatase-conjugated anti-human IgA and horseradish peroxidase-conjugated anti-human IgG (diluted in PBS containing 0.1% Tween 20 and 1% BSA) antibody (Jackson Immunoresearch, West Grove, PA) for 2 hours at 37°C in 5% CO2 and then developed with VECTOR Blue ...
-
bioRxiv - Cancer Biology 2019Quote: ... antibody binding was detected by incubation with peroxidase-conjugated goat anti-human IgG (F(ab’)2 fragment specific) (Jackson ImmunoResearch, West Grove, PA) and TMB solution ...
-
bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
-
bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories, cat. 172-1050, or Jackson ImmunoResearch, West Grove, PA, USA, cat. 309-035-003) was added and incubated for 2 h at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... Plasma binding to cell-surface Spike was revealed using fluorescent secondary antibodies able to detect all Ig isotypes (anti-human IgM+IgG+IgA; Jackson ImmunoResearch Laboratories, Inc.) or specific to IgG isotype (Biolegend) ...
-
bioRxiv - Immunology 2022Quote: ... coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-170) and goat anti-human IgA-Cy3 (Jackson ImmunoResearch ...
-
bioRxiv - Immunology 2020Quote: ... The plates were incubated for 1 hour at RT followed by washing and addition of 100 μl of anti-human IgA + IgG + IgM (H+L) conjugated to HRP (Jackson ImmunoResearch Laboratories, Inc.) diluted 1:50 000 in blocking buffer ...
-
bioRxiv - Immunology 2021Quote: ... Antibody binding to the beads was detected with 2.5 µg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170), goat anti-human IgA-Cy3 (Jackson Immunoresearch 109-166-011 ...
-
bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
-
bioRxiv - Microbiology 2021Quote: ... to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
-
bioRxiv - Immunology 2020Quote: ... The wells were washed five times with PBS and probed with horseradish peroxidase (HRP)-conjugated secondary antibody [goat anti-human IgG (Jackson ImmunoResearch; 1:5000)] for 1 h ...
-
bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
-
bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
-
bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:200 and donkey F(ab’)2 anti-human IgM Fc5μ at 1:400 dilution (1 mg/ml, Jackson ImmunoResearch Laboratories, Inc.) was used as the secondary antibody for detection.
-
bioRxiv - Immunology 2020Quote: ... bound IgG molecules were revealed with peroxidase conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, 1/10 000 in 1% chicken egg albumin) and TMB reagent ...
-
bioRxiv - Immunology 2021Quote: ... and HRP-conjugated goat polyclonal anti-human IgG F(ab’)2 fragment antibodies (Jackson ImmunoResearch 109-036-008, 1:5000 dilution in PBSM) were used as secondary detection antibodies for sera and Motavizumab ...
-
bioRxiv - Immunology 2021Quote: ... Bound IgG was detected using 1μg/mL red phycoerythrin (R-PE)-conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, Inc., West Grove, PA) and resuspended in MAGPIX® drive fluid before being analyzed on a MAGPIX® Instrument (Luminex Corporation ...
-
bioRxiv - Immunology 2022Quote: ... and incubated for 1 hr at RT.Detection was measured with alkaline phosphatase-conjugated goat anti-human IgG Fcγ (Jackson ImmunoResearch 109-005- 008) at 1:2000 dilution in 1% BSA in PBS for 1hr ...
-
bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
-
bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgG Fc-gamma (Jackson ImmunoResearch 109-135-098) to label for bound NHP antibodies or Alexa Fluor-647-conjugated goat anti-mouse-IgG Fc-gamma (Jackson ImmunoResearch 115-605-008 ...
-
bioRxiv - Physiology 2023Quote: ... incubated for 90 min at room temperature with a donkey anti-human CyTM 3 antibody (1:500; Jackson ImmunoResearch, Cat #709-165-149) and coverslipped ...
-
bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
-
bioRxiv - Bioengineering 2023Quote: ... The plates were washed four times and then incubated with Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) at room temperature for 1 hour ...
-
bioRxiv - Immunology 2023Quote: ... clone CMYC-45F) to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch, cat. 109-115-098) to label for bound mAb.
-
bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
-
bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...
-
bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
-
bioRxiv - Immunology 2021Quote: ... The plates were then washed once with 200µl of FACS buffer and stained with of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat# 109-117-008) at a 1:100 dilution and live/dead green fluorescent reactive dye (Thermo Fisher Cat# L34970 ...
-
bioRxiv - Immunology 2021Quote: ... Plates were developed using 15μl/well of combination of horseradish peroxidase–conjugated antibodies in assay diluent comprising) goat anti-human IgG (Jackson ImmunoResearch Laboratories,109-035-098) at 1:10,000 dilution ...
-
bioRxiv - Neuroscience 2020Quote: ... murine recPrP23-110 and human recPrP121-230 and BSA (coated at 43 nM) in ELISA using goat anti-human IgG Fc-gamma specific-HRP (Jackson ImmunoResearch #109-035-098), 1:4’000 for detection.
-
bioRxiv - Microbiology 2020Quote: ... followed by secondary labeling with 1:100 FITC-conjugated anti-Myc to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
-
bioRxiv - Immunology 2020Quote: ... 1µg of each mAb was complexed with 3µg of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat #109-117-008), and the labeled mAb was incubated for 30 min at RT prior to dilution to 5µg/mL in Freestyle medium containing 10% FBS and 1% Pen/Strep ...
-
bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/30 000) or goat anti-human IgA (α-chain specific) HRP conjugate (1/10 000) (all from Jackson ImmunoResearch Laboratories, Inc.) were added and samples were incubated at 37°C for 1h ...
-
bioRxiv - Immunology 2021Quote: ... for 1 h at room temperature followed by incubation for 1h at room temperature with anti-human peroxidase conjugate IgG (Jackson ImmunoResearch, Ely, Cambridgeshire, UK) or with anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 μl of 5 μg/ml HIV-1 Env gp140 SF162.B (NIH AIDS Reagent Program) or 1 μg/ml anti-human IgG or IgM (Jackson Immunoresearch, West Grove, PA) in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... the arrays were washed with T-TBS three times followed by the incubation with goat anti-human IgG+IgA+IgM pAb or donkey-anti-rabbit IgG (HRP conjugates, 1:20,000 dilution; Jackson ImmunoResearch, West Grove, Pennsylvania, USA). After 30 min incubation ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by a 1 hour incubation with a 1:2000 dilution of anti-human IgG-HRP (#109-035-088, Jackson ImmunoResearch, West Grove, PA) for Her2-Fc detection ...
-
bioRxiv - Microbiology 2022Quote: ... incubated with 1:100 FITC-conjugated chicken anti-Myc antibody to label RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label bound antibody ...
-
bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... an ELISA assay was developed to detect LH01 or un-cleaved LH05 with IL-15Rβ coated on the plate and Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) as the detection antibody ...