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Citations for Jackson ImmunoResearch :
401 - 450 of 511 citations for Recombinant Human CD244 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated with goat anti-human Fc fragment specific antibody for E-cadherin substrate (Jackson ImmunoResearch, West Grove, PA), at 1 μg / cm2 in 0.1 M pH 8 borate buffer at 4°C overnight in a humidity chamber ...
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bioRxiv - Microbiology 2020Quote: ... the arrays were washed three times with T-TBS and then incubated for 45 min at RT with biotin-conjugated anti-human IgG secondary antibody (Jackson ImmunoResearch), diluted at 1/400 in array blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... Allophycocyanin-conjugated F(ab′)2 goat anti-mouse or donkey anti-human IgG (H+L) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Immunology 2021Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2020Quote: ... Soluble ICAM1-Fc-F(ab’)2 complexes were generated by diluting APC-labeled goat anti-human IgG F(ab’)2 fragments (109-135-098, Jackson Immunoresearch) 1:6.25 with ICAM1-Fc (200μg/ml final ...
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bioRxiv - Immunology 2020Quote: ... Bound SARS-CoV-2 IgG antibodies were then detected by incubating for 30 min at 37°C with a secondary AF488-conjugated AffiniPure goat anti-human IgG antibody (Jackson Immunoresearch), diluted 1:200 in 0.1% Evan’s Blue ...
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bioRxiv - Immunology 2021Quote: ... Either soluble ICAM-1-Fc-F(ab’)2 or VCAM-1-Fc-F(ab’)2 complexes were generated by diluting APC-labelled goat anti-human IgG F(ab’)2 fragments (109-135-098, Jackson Immunoresearch) 1:6.25 with either ICAM-1-Fc (200µg/ml final ...
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bioRxiv - Immunology 2021Quote: ... Coverslips were rinsed and directly immunostained with anti-Env 2G12 or PGT145 bNAbs and AlexaFluor 647 Goat anti-human IgG secondary Ab (Jackson ImmunoResearch) prior to fixation ...
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bioRxiv - Immunology 2021Quote: ... and incubated for four hours in the dark at room temperature with 1ug/ml goat anti-human IgG horse radish peroxidase antibody (Jackson Immunoresearch). Cells were again washed three times with PBS-Tween 20 (0.5% ...
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bioRxiv - Immunology 2020Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2021Quote: ... and recombinant mouse IL21R Fc Chimera (600x; R&D) followed by PE-labeled affinity-purified F(ab’)2 fragment of goat anti-human Fcγ (Jackson ImmunoResearch). For detection of phosphorylated antigens ...
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bioRxiv - Microbiology 2021Quote: ... Membranes overlaid with rH3-Fc were subsequently probed with biotin-SP-conjugated AffiniPure goat anti-human IgG (Jackson ImmunoResearch; 1:2000). Immunodetection was performed using infrared dye-labeled secondary antibodies (IRDye 800CW Biosciences ...
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bioRxiv - Bioengineering 2020Quote: ... donkey anti-human IgG–HRP conjugate (709-035-149) and goat anti-mouse IgG-HRP conjugate (115-035-071) from Jackson ImmunoResearch; mouse anti-human ErbB-2 (CD340 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and then incubated for 1 hr in blocking buffer without NaN3 to which of horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-human IgG Fc specific antibody (Jackson Immunoresearch) had been added at the dilution as recommended by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... washed and incubated for another 30 min at 4°C with APC-labelled donkey-anti-human-IgG (Jackson ImmunoResearch Europe Ltd.). For complement deposition assays ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with PBS containing 3% normal donkey serum and 0.3% Triton X-100 and then incubated with 100 μg/ml unconjugated anti-human IgG Fab fragments (H+L, Jackson Immunoresearch). Slides were then incubated with FM or HC serum diluted 1:500 in PBS with 1% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed six times and total IgG binding was measured in a 1-h incubation with 100 μL/well of 1:50,000 HRP-conjugated polyclonal goat anti-human IgG (Jackson ImmunoResearch Labs) and developed using 50 μL/well TMB substrate (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Bound ICs were detected using a PE-conjugated goat anti-human IgG F(ab’)2 fragment at 0.5 μg/ml (Jackson ImmunoResearch Laboratories) on a BD FACSCanto II flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... The plates were then washed three times with PBS containing 0.05% Tween 20 and incubated for 1 hr with a dilution of a 1/1,000 horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch Laboratories), developed with TMB Peroxidase Substrate (SeraCare) ...
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bioRxiv - Immunology 2023Quote: ... Binding of soluble ICAM1-Fc-F(ab’)2 complexes were generated by diluting APC-labeled goat anti-human IgG F(ab’)2 fragments (109-135-098, Jackson Immunoresearch) 1:6.25 with rmICAM1-Fc (200µg/ml final ...
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bioRxiv - Immunology 2023Quote: ... All plasma samples were heat-inactivated before usage to minimize risk of residual virus in serum and then incubated at serial dilution followed by multiple washes and incubation with horseradish peroxidase-conjugated secondary Goat Anti-Human secondary IgG (Cat No: 109-035-008, Jackson ImmunoResearch) at 1:40,000 dilution in 3% milk at 37°C for 1 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then incubated with horseradish peroxidase (HRPO) conjugated donkey anti-human IgG (Fcγ fragment specific) antibody (Jackson ImmunoResearch Inc., West Grove, PA), and developed using 3,3’,5,5’-tetramethylbenzidine (TMB/ Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed once in PBS before IgG was detected with a 1:1000 dilution of goat anti-human IgG antibody labelled with the BV421 fluorophore (Jackson ImmunoResearch) or C3 was detected with a 1:1000 dilution of goat anti-human C3 F(ab’)2 labelled with FITC (Protos Immunoresearch) ...
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bioRxiv - Immunology 2023Quote: ... Plates were then washed twice with Superwash and the corresponding IgG-HRP secondary antibody (goat anti mouse IgG-HRP or goat anti human IgG-HRP; Jackson Immunoresearch) diluted in SuperBlock (without Sodium azide ...
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bioRxiv - Immunology 2024Quote: ... Presence of IgM was detected using a goat anti-human IgM (Fc5u specific) secondary antibody conjugated to alkaline phosphatase (Jackson ImmunoResearch) (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Purified human naïve B cells were cultured in 96-well plates in the presence of AffiniPure F(ab’)₂ Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109-006-127, Jackson ImmunoResearch), anti-human CD40 (5 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-mouse TRITC and donkey anti-human Cy5 (1:300, 711-095-152, 111-025-006, 715-025-150, 715-095-150, 709-175-149, Jackson Immunoresearch). Coverslips were mounted on glass slides using Vectashield® anti-fade mounting medium with DAPI staining.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then stimulated with 10 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L) (Jackson ImmunoResearch, USA) for the indicated time points ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L) were obtained from Jackson ImmunoResearch Inc (USA) ...
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bioRxiv - Immunology 2022Quote: ... and Ab49 and stained with fluorescently labelled IgGFab or IgGFc specific F(ab’)2 fragments (DyLight488-conjugated anti-human IgGFc or IgGFab; Jackson ImmunoResearch Laboratory). Samples were mounted on glass slides using Prolong Gold Antifade Mountant with No ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µl of AffiniPure goat anti-human IgG Fcγ fragment specific antibody (for IgG samples, Jackson ImmunoResearch Laboratories Inc, 109-005-098) in media was added to yield a 1:1 molar ratio with each antibody dilution ...
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bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound serum antibodies ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight with 250 ng/well of purified goat anti-human IgA or IgG antibody (Jackson ImmunoResearch, 0.8 µg/ml final). After washings ...
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bioRxiv - Immunology 2020Quote: ... The antibody samples were stained with fluorescently labeled IgGFab or IgGFc specific F(ab’)2 fragments (AlexaFluor647-conjugated anti-human IgGFc or IgGFab; Jackson ImmunoResearch Laboratory). Samples were set on glass slides using Prolong™ Gold Antifade Mountant with No ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies IgG (cat no. 109-605-098, Alexa Fluor 647-AffiniPure Goat Anti-Human IgG, Fcy fragment specific, Jackson ImmunoResearch Labs) and IgM (cat no ...
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bioRxiv - Microbiology 2021Quote: Allophycocyanin (APC)-conjugated donkey anti-mouse IgG Fc fragment antibody and APC-conjugated anti-human IgG Fc fragment specific antibody (Jackson ImmunoResearch, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Immunology 2020Quote: ... Slides were probed with a mixture of secondary antibodies consisting of Cy3-labeled goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:2,000 and Cy5-labeled goat anti-human IgM (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... a secondary antibody conjugated with alkaline phosphatase (AffiniPure goat anti-human IgG Fc fragment specific, Jackson ImmunoResearch Laboratories cat. #109-055-008) diluted 1:1000 in 1% BSA/PBS-T ...
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bioRxiv - Immunology 2022Quote: ... Proper CAR transduction was checked by flow cytometry by eGFP expression and by using AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch Laboratories).
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bioRxiv - Immunology 2022Quote: ... The transfectant cells were incubated with supernatant containing expressed antibodies for 30 minutes then followed by incubation with APC-anti-human IgG (H+L) antibody (Jackson ImmunoResearch, USA). Bound antibodies to the GFP-positive cells were then analyzed by flow cytometry (Attune NxT ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with an HRP-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, 109-035-003; 1:1000 dilution in TBST) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with Allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (1:100 dilution; catalog number 709-136-149; Jackson ImmunoResearch Laboratories) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were then detected using 1:2000 diluted alkaline phosphatase-labelled anti-human IgG Fcγ (Jackson ImmunoResearch Laboratories #109-055-098) and 4-Nitrophenyl phosphate disodium salt hexahydrate substrate (Sigma #S0942) ...
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bioRxiv - Immunology 2023Quote: ... and then incubated with a 1:2500 dilution of horseradish peroxidase-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, West Grove, PA) in blocking buffer for one hour ...
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bioRxiv - Pathology 2024Quote: ... the mAb reactivity was detected by mean of a Cy3-conjugated goat anti-human IgG serum (Jackson Immunoresearch Laboratories, West Grove, PA), diluted 1:1000 in 0.1% BSA in HBS ...
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected with 50 μl of 1 μg/ml alkaline phosphatase-conjugated anti-human IgA and horseradish peroxidase-conjugated anti-human IgG (diluted in PBS containing 0.1% Tween 20 and 1% BSA) antibody (Jackson Immunoresearch, West Grove, PA) for 2 hours at 37°C in 5% CO2 and then developed with VECTOR Blue ...
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bioRxiv - Cancer Biology 2019Quote: ... antibody binding was detected by incubation with peroxidase-conjugated goat anti-human IgG (F(ab’)2 fragment specific) (Jackson ImmunoResearch, West Grove, PA) and TMB solution ...
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bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories, cat. 172-1050, or Jackson ImmunoResearch, West Grove, PA, USA, cat. 309-035-003) was added and incubated for 2 h at 37 °C ...