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Citations for Jackson ImmunoResearch :
401 - 450 of 490 citations for MECR Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Samples were washed once in PBS before IgG was detected with a 1:1000 dilution of goat anti-human IgG antibody labelled with the BV421 fluorophore (Jackson ImmunoResearch) or C3 was detected with a 1:1000 dilution of goat anti-human C3 F(ab’)2 labelled with FITC (Protos Immunoresearch) ...
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bioRxiv - Immunology 2023Quote: ... Plates were then washed twice with Superwash and the corresponding IgG-HRP secondary antibody (goat anti mouse IgG-HRP or goat anti human IgG-HRP; Jackson Immunoresearch) diluted in SuperBlock (without Sodium azide ...
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bioRxiv - Immunology 2024Quote: ... Presence of IgM was detected using a goat anti-human IgM (Fc5u specific) secondary antibody conjugated to alkaline phosphatase (Jackson ImmunoResearch) (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Purified human naïve B cells were cultured in 96-well plates in the presence of AffiniPure F(ab’)₂ Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109-006-127, Jackson ImmunoResearch), anti-human CD40 (5 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-mouse TRITC and donkey anti-human Cy5 (1:300, 711-095-152, 111-025-006, 715-025-150, 715-095-150, 709-175-149, Jackson Immunoresearch). Coverslips were mounted on glass slides using Vectashield® anti-fade mounting medium with DAPI staining.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then stimulated with 10 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L) (Jackson ImmunoResearch, USA) for the indicated time points ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L) were obtained from Jackson ImmunoResearch Inc (USA) ...
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bioRxiv - Immunology 2022Quote: ... and Ab49 and stained with fluorescently labelled IgGFab or IgGFc specific F(ab’)2 fragments (DyLight488-conjugated anti-human IgGFc or IgGFab; Jackson ImmunoResearch Laboratory). Samples were mounted on glass slides using Prolong Gold Antifade Mountant with No ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µl of AffiniPure goat anti-human IgG Fcγ fragment specific antibody (for IgG samples, Jackson ImmunoResearch Laboratories Inc, 109-005-098) in media was added to yield a 1:1 molar ratio with each antibody dilution ...
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bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound serum antibodies ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight with 250 ng/well of purified goat anti-human IgA or IgG antibody (Jackson ImmunoResearch, 0.8 µg/ml final). After washings ...
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bioRxiv - Immunology 2020Quote: ... The antibody samples were stained with fluorescently labeled IgGFab or IgGFc specific F(ab’)2 fragments (AlexaFluor647-conjugated anti-human IgGFc or IgGFab; Jackson ImmunoResearch Laboratory). Samples were set on glass slides using Prolong™ Gold Antifade Mountant with No ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies IgG (cat no. 109-605-098, Alexa Fluor 647-AffiniPure Goat Anti-Human IgG, Fcy fragment specific, Jackson ImmunoResearch Labs) and IgM (cat no ...
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bioRxiv - Microbiology 2021Quote: Allophycocyanin (APC)-conjugated donkey anti-mouse IgG Fc fragment antibody and APC-conjugated anti-human IgG Fc fragment specific antibody (Jackson ImmunoResearch, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Immunology 2020Quote: ... Slides were probed with a mixture of secondary antibodies consisting of Cy3-labeled goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:2,000 and Cy5-labeled goat anti-human IgM (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... a secondary antibody conjugated with alkaline phosphatase (AffiniPure goat anti-human IgG Fc fragment specific, Jackson ImmunoResearch Laboratories cat. #109-055-008) diluted 1:1000 in 1% BSA/PBS-T ...
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bioRxiv - Immunology 2022Quote: ... Proper CAR transduction was checked by flow cytometry by eGFP expression and by using AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch Laboratories).
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bioRxiv - Immunology 2022Quote: ... The transfectant cells were incubated with supernatant containing expressed antibodies for 30 minutes then followed by incubation with APC-anti-human IgG (H+L) antibody (Jackson ImmunoResearch, USA). Bound antibodies to the GFP-positive cells were then analyzed by flow cytometry (Attune NxT ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with an HRP-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, 109-035-003; 1:1000 dilution in TBST) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with Allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (1:100 dilution; catalog number 709-136-149; Jackson ImmunoResearch Laboratories) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were then detected using 1:2000 diluted alkaline phosphatase-labelled anti-human IgG Fcγ (Jackson ImmunoResearch Laboratories #109-055-098) and 4-Nitrophenyl phosphate disodium salt hexahydrate substrate (Sigma #S0942) ...
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bioRxiv - Immunology 2023Quote: ... and then incubated with a 1:2500 dilution of horseradish peroxidase-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, West Grove, PA) in blocking buffer for one hour ...
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bioRxiv - Pathology 2024Quote: ... the mAb reactivity was detected by mean of a Cy3-conjugated goat anti-human IgG serum (Jackson Immunoresearch Laboratories, West Grove, PA), diluted 1:1000 in 0.1% BSA in HBS ...
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected with 50 μl of 1 μg/ml alkaline phosphatase-conjugated anti-human IgA and horseradish peroxidase-conjugated anti-human IgG (diluted in PBS containing 0.1% Tween 20 and 1% BSA) antibody (Jackson Immunoresearch, West Grove, PA) for 2 hours at 37°C in 5% CO2 and then developed with VECTOR Blue ...
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bioRxiv - Cancer Biology 2019Quote: ... antibody binding was detected by incubation with peroxidase-conjugated goat anti-human IgG (F(ab’)2 fragment specific) (Jackson ImmunoResearch, West Grove, PA) and TMB solution ...
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bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories, cat. 172-1050, or Jackson ImmunoResearch, West Grove, PA, USA, cat. 309-035-003) was added and incubated for 2 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Plasma binding to cell-surface Spike was revealed using fluorescent secondary antibodies able to detect all Ig isotypes (anti-human IgM+IgG+IgA; Jackson ImmunoResearch Laboratories, Inc.) or specific to IgG isotype (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-170) and goat anti-human IgA-Cy3 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated for 1 hour at RT followed by washing and addition of 100 μl of anti-human IgA + IgG + IgM (H+L) conjugated to HRP (Jackson ImmunoResearch Laboratories, Inc.) diluted 1:50 000 in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding to the beads was detected with 2.5 µg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170), goat anti-human IgA-Cy3 (Jackson Immunoresearch 109-166-011 ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2021Quote: ... to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Immunology 2020Quote: ... The wells were washed five times with PBS and probed with horseradish peroxidase (HRP)-conjugated secondary antibody [goat anti-human IgG (Jackson ImmunoResearch; 1:5000)] for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:200 and donkey F(ab’)2 anti-human IgM Fc5μ at 1:400 dilution (1 mg/ml, Jackson ImmunoResearch Laboratories, Inc.) was used as the secondary antibody for detection.
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bioRxiv - Immunology 2020Quote: ... bound IgG molecules were revealed with peroxidase conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, 1/10 000 in 1% chicken egg albumin) and TMB reagent ...
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bioRxiv - Immunology 2021Quote: ... and HRP-conjugated goat polyclonal anti-human IgG F(ab’)2 fragment antibodies (Jackson ImmunoResearch 109-036-008, 1:5000 dilution in PBSM) were used as secondary detection antibodies for sera and Motavizumab ...
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bioRxiv - Immunology 2021Quote: ... Bound IgG was detected using 1μg/mL red phycoerythrin (R-PE)-conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, Inc., West Grove, PA) and resuspended in MAGPIX® drive fluid before being analyzed on a MAGPIX® Instrument (Luminex Corporation ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hr at RT.Detection was measured with alkaline phosphatase-conjugated goat anti-human IgG Fcγ (Jackson ImmunoResearch 109-005- 008) at 1:2000 dilution in 1% BSA in PBS for 1hr ...
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgG Fc-gamma (Jackson ImmunoResearch 109-135-098) to label for bound NHP antibodies or Alexa Fluor-647-conjugated goat anti-mouse-IgG Fc-gamma (Jackson ImmunoResearch 115-605-008 ...
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bioRxiv - Physiology 2023Quote: ... incubated for 90 min at room temperature with a donkey anti-human CyTM 3 antibody (1:500; Jackson ImmunoResearch, Cat #709-165-149) and coverslipped ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were washed four times and then incubated with Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... clone CMYC-45F) to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch, cat. 109-115-098) to label for bound mAb.
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...