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Citations for Jackson ImmunoResearch :
251 - 300 of 848 citations for Human Neuropilin 2 NRP 2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were then stimulated with nicotine for 20 min and subsequently anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch) for 2 min (pCD19 ...
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bioRxiv - Immunology 2020Quote: ... Goat anti-human anti-IgG (Goat anti-human IgG Fcy-HRP, Jackson Immunoresearch, #109-035-098) at a 1:60,000 dilution (0.67 ng/well ...
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bioRxiv - Molecular Biology 2019Quote: ... Human IgG (equal amount, Jackson ImmunoResearch) was used as the control ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-human IgG (Jackson ImmunoResearch), Streptavidin (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked for 1 hour in PFN buffer (PBS without Ca2+ and Mg2+ and containing 10% FBS and 0.02% azide) with 2% normal goat serum (Jackson ImmunoResearch). Cells were incubated for 1 hour with the appropriate primary antibodies diluted in blocking medium ...
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bioRxiv - Bioengineering 2021Quote: ... we labeled transduced T cells with Alexa Fluor 647- labeled rabbit anti-mouse F(ab)2 antibody from Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with anti-rabbit-Cy2 and anti-rat-Cy3 secondary antibodies for 2 h (1:400, Jackson Immunoresearch, #711-225-152 ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was incubated for 24 h at 4°C in a blocking solution [TBS with 0.3% Triton X-100 and 2% normal donkey serum (017-000-121, Jackson ImmunoResearch)] with the primary antibody anti-cFos raised in rabbit (1:1K ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibody against RFP was detected with a 2-hour incubation in diluted donkey-anti-rabbit-IgG-Cy3 antibody in blocking buffer (1:1,000, Jackson ImmunoResearch). Finally ...
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bioRxiv - Microbiology 2021Quote: ... and for multiplex beadbased assay with anti-mouse IgG (Fc-specific) F(ab’)2 PE (Jackson ImmunoResearch, West Grove PA). Data were acquired on a Magpix instrument (Luminex ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation (2 h) with appropriate fluorescent secondary antibodies (Cy3-or Cy2-coupled fluorescent secondary antibodies, 1:200; Jackson ImmunoResearch Laboratories catalog #715-165-150 and #715-545-150 respectively) ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 2 hours with a species-specific secondary antibody conjugated with green Alexa fluorophore (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... After 3x washing in PBT (with 0.1% Tween 20) embryos were incubated for 2 hours at room temperature with secondary antibodies from Jackson Immunoresearch. Vectashield with DAPI or TSO was used as mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... MilliporeSigma) was applied for 2 h at RT followed by secondary antibody (fluorophore-conjugated IgG, 1:500; Jackson ImmunoResearch Laboratories) for 1 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... AffiniPure F(ab’)2-goat anti-rat IgG (H+L) Alexa 488 (Jackson ImmunoResearch Labs Cat# 112-546-003, RRID:AB_2338364), F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109 ...
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bioRxiv - Bioengineering 2023Quote: ... and a 1000x dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson Immunoresearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Cell Biology 2023Quote: ... or allophycocyanin-conjugated (APC) AffiniPure F (ab’)2 fragment goat anti-Rat IgM (1:100, Jackson ImmunoResearch, 112-136-075) at 4°C for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... permeabilization and and blocking were performed with PBS (with 0.4% (v/v) Triton X-100%) and 2% immunoglobulin G-free BSA (Jackson ImmunoResearch) for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 mM EDTA) and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003 ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with either 2 µg/ml of donkey anti-mouse IgG (Affinipure; Jackson ImmunoResearch Laboratories, West Grove, PA), carbonate buffer (0.0875 M Na2CO3 ...
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bioRxiv - Immunology 2023Quote: ... the TCR was stimulated by addition of 15 μg/ml of the CD3 antibody clone UCHT1 or alternatively by restimulation with CD3 mAbs (TR66 clone) and crosslinking by 1.3 μg/mL anti-mouse IgGF(ab)’2 fragments (Jackson ImmunoResearch). The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... cells were stimulated with a suboptimal concentration of pre-mixed complexes composed of 0.056 µg mL-1 biotinylated F(ab’)2 goat anti-mouse IgM (µ-chain specific) (Jackson ImmunoResearch), ∼3 µg mL-1 C3dg-biotin (produced in house ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with secondary antibodies R-Phycoerythrin AffiniPure F (ab’) 2 Fragment Donkey Anti-Rat IgG (1:300; 712-116-153; Jackson ImmunoResearch) or Alexa Fluor 568 donkey anti-rabbit IgG (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in a secondary antibody solution at room temperature for 2 h with protection from light (1:500, Alexa Fluor 647 donkey anti-chicken, Jackson Immunoresearch, USA ...
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bioRxiv - Neuroscience 2021Quote: ... tissue is washed 10 times in 1X PBS for 1 min each and then incubated for 2 hours in blocking buffer + secondary antibodies from Jackson ImmunoResearch Laboratories (donkey anti-rabbit 488 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2–4 h with a mixture of fluorophore-conjugated secondary antibodies (all raised in donkey, 1:500, Jackson Immunoresearch): Anti-guinea pig IgG (DyLight 488 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for ∼ 2 h at room temperature with the appropriate AlexaFluor or Dylight 488/568/647 conjugated secondary antibodies (ThermoFisher / Jackson ImmunoResearch) and counterstained with DAPI ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and Peroxidase-AffiniPure F(ab’)2 Fragment Goat anti-mouse IgG (111-036-047, Jackson ImmunoResearch, USA) diluted to 1:20,000 in blocking buffer was added to every well and incubated for 1 hr using a plate shaker set to 450 rpm at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... for detection of primary rabbit and Peroxidase F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (715-036-150, Jackson ImmunoResearch) for detection of primary mouse antibodies were diluted in Blocking Buffer 1:1000 respectively ...
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bioRxiv - Developmental Biology 2020Quote: Tissue was blocked for an hour at room temperature or overnight at 4°C in phosphate buffered saline with 2% Normal Donkey Serum (Jackson ImmunoResearch), followed by 2 hours at room temperature in primary antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... and adding 25 μl of pre-diluted labelled antibody to 2 μl of donkey anti-rabbit IgG (711-005152, Jackson ImmunoResearch) coupled beads followed by incubation for 1 h at 650 rpm at room temperature ...
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bioRxiv - Neuroscience 2019Quote: We visualized primary anti-AmOA1 using F(ab’)2 fragments of donkey anti-goat antibodies conjugated to Cy5 (Jackson ImmunoResearch Laboratories). F(ab’)2 fragments of donkey anti-mouse antibodies conjugated to Alexa 488 were used to visualize anti-synapsin (Jackson ImmunoResearch Laboratories).
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bioRxiv - Neuroscience 2019Quote: ... the neurons were permeabilized and blocked with 0.4% saponin and 2% normal goat serum (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) in PBS at room temperature for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated for 1 hour at room temperature with HRP conjugated-(Fab)2 Goat antimouse IgG (Fc specific) or Goat anti-mouse IgM (both 1/10000, Jackson Immunoresearch). Then ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were incubated with F(ab’)2 goat-anti-mouse IgM+G (10 μg/ml, Jackson ImmunoResearch Laboratories) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (Thermo Fisher Scientific or Jackson Immunoresearch). Nuclei were labeled with DAPI ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed at indicated time points in 2% paraformaldehydestained with fluorescently conjugated antibodie Fab fragments targeting IgM (Jackson Immunoresearch) and analysed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... We used the following secondary antibodies: F(ab’)2 conjugates with FITC Donkey Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch 706-096-148 ...
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bioRxiv - Biochemistry 2022Quote: ... The horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and phycoerythrin (PE)-anti-rabbit F(ab’)2 antibody was obtained from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2022Quote: Flat-bottom plate were coated with 20 μg/ml AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L)mouse (Jackson Immunoresearch) in PBS for two hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Purity of the F[ab] fragments was assessed by Western blot analysis with 100 ng of sample per well and direct probing with anti-IgG F[ab]2 and anti-IgG Fc conjugated to horseradish peroxidase (Jackson ImmunoResearch).
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed 3 × 5 minutes in PBS prior to a 2 hour incubation with a biotin-conjugated donkey anti-rat secondary antibody (1:500, 712-065-153, Jackson ImmunoResearch). The primary–secondary antibody complexes were detected via peroxidase-driven precipitation of diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2021Quote: ... RRID: AB_2532109) in PBS with 0.5% Triton X-100 (MilliporeSigma) and 2% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA). Sections were then washed in PBS and incubated for 90 min at room temperature in Alexa Fluor 488- or 594-conjugated donkey anti-mouse IgG (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... Several rinsing with TBS was followed by incubation in secondary antibody solution for 2 hours (1:500 Cy3 conjugated goat anti-rabbit IgG, #134845, Jackson ImmunoResearch).
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bioRxiv - Immunology 2021Quote: ... Cy-5-conjugated donkey anti-rabbit IgG and Alexa fluor-488 F(ab’)2 fragment goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratories (Cambridgeshire ...
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bioRxiv - Immunology 2019Quote: ... using anti-CD43 beads and magnetic columns (Milteyni Biotec) and stimulated in vitro with 10 μgml− F(ab)2 fragment of goat anti-mouse IgM (Jackson ImmunoResearch) in combination with 25U ml− recombinant mouse IL-4 (R&D) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each slide was then incubated in the dark for 2 hours at room temperature with a 1:1000 dilution of Alexa-Fluor 488 goat anti-mouse secondary antibody (Jackson ImmunoResearch) diluted in 1x PBS + 0.1% Tween-20 ...
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bioRxiv - Immunology 2019Quote: ... and 20 μg/ml Alexa Fluor 647 labelled donkey anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch, 715-606-020) in imaging buffer (0.5mM CaCl2 ...