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Citations for Jackson ImmunoResearch :
551 - 600 of 683 citations for Galectin 3 LGALS3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgG Fc-gamma (Jackson ImmunoResearch 109-135-098) to label for bound NHP antibodies or Alexa Fluor-647-conjugated goat anti-mouse-IgG Fc-gamma (Jackson ImmunoResearch 115-605-008 ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were washed four times and then incubated with Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... clone CMYC-45F) to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch, cat. 109-115-098) to label for bound mAb.
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... 1:75,000) against human kisspeptin-54 (aa 68-121 of NP_002247.3) (9, 53) using biotinylated secondary antibodies (Jackson ImmunoResearch Europe, Cambridgeshire, UK; 1:500), the ABC Elite reagent (Vector ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in a blocking solution composed of PBST with 3% normal donkey serum (Jackson ImmunoResearch, #017-000-121) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed for 15 min with 1x PBS/0.1% Tween20 post primary antibody treatment followed by 1 hour incubation at room temperature with 1x PBS+3%BSA containing secondary antibodies anti rabbit IgG-Alexa 488 or anti rat IgG-Alexa 594 (all from Jackson ImmunoResearch). The nuclear stain DAPI (2μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was incubated for 3 hrs at room temp with biotinylated donkey anti-rabbit (1:500, Jackson Immunoresearch 711-065-152), then washed ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rinsed three times in 1X PBS and incubated in secondary antibody solution Cy-3 donkey anti-rabbit (1:500; Jackson ImmunoResearch) or Cy-3 donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated for 3-h at room RT in biotin-streptavidin conjugated AffiniPure goat anti-rabbit IgG (1:200; Jackson Immunoresearch), washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed with 0.01M PBS (10 Min × 3 times) and incubated in FITC or texas red-conjugated secondary antibody (1:200, Jackson ImmunoResearch Laboratories) in dilution buffer (1% BSA and 0.3% Triton X-100 in 0.01M PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... brain slices were incubated with the following secondary antibodies in the blocking solution at RT 3 hours: donkey anti-mouse-AlexaFluor 488 (715-545-150; 1:300; Jackson ImmunoResearch), donkey anti-G.P.-RRX (706-295-148 ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated for 3 h at room temperature with 1:250 anti-mouse DyLight 650 (Jackson ImmunoResearch, Burlington, ON, Canada) secondary antibody diluted in 0.1 M PB containing 1.5% heat inactivated horse serum ...
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bioRxiv - Cell Biology 2022Quote: ... 2016)) and secondary antibodies were anti-rat coupled to Alexa Fluor 488 and anti-rabbit coupled to Cyanine 3 (Jackson ImmunoResearch) both diluted to 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were then incubated for 60 min at 37°C with the respective secondary antibodies diluted in 3% BSA: AMCA-conjugated anti-chicken (1:200; Jackson ImmunoResearch), Alexa Fluor® 488 conjugated anti-rabbit (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed 3 times for 5 min in PBS and incubated for 30 min with a cyanine 3-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch) and DAPI (4′,6-diamidino-2-phenylindole).
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bioRxiv - Biophysics 2019Quote: ... Cells were then rinsed three times with PBS and permeabilized with blocking buffer (3% bovine serum albumin (BSA, 001-000-162, Jackson ImmunoResearch) and 0.2% Triton X-100 (X100 ...
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bioRxiv - Neuroscience 2020Quote: ... the brains were washed three times at room temperature in 0.25% PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch 115-165-062 ...
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bioRxiv - Neuroscience 2020Quote: ... they were washed 3 times with 1X PBS and then incubated with 1:1000 secondary antibodies (Donkey-anti-Rat Cy3, Jackson ImmunoResearch 712-165-153 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 3 hr in secondary antibody (anti-Chicken AlexaFluor 488 and anti-Rabbit Cy3, each 1:250, Jackson ImmunoResearch) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed with PBS 3 times and incubated with a secondary antibody (Alexa 488 anti-chicken, 703-545-155, Jackson ImmunoResearch) for 1 h at RT and then washed with PBS 3 times for 10 m ...
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bioRxiv - Microbiology 2023Quote: ... and blocked in 3% milk– PBS-T prior to incubation with serial dilutions of serum or the mouse IgG standard (Invitrogen, Jackson ImmunoResearch) in 1% milk-PBS-T for 2 hrs at RT ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then washed 3 times for 10 min with PBS and stained with the suitable secondary antibody (1:200, Jackson ImmunoResearch) and DAPI (4,6-diamidino-2-phenylindole dihydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated for 3 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (1:1,000; Thermo Fisher Scientific or Jackson ImmunoResearch). Whole retinas were flattened with photoreceptor side down onto nitrocellulose filters ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... followed by a 3-h incubation with polyclonal Donkey anti-Goat Alexa flour 488 antibody (AB 2340428, Jackson immunoresearch, PA, USA). These antibodies were diluted by ×500 ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Neuroscience 2023Quote: ... one slice for each animal for each treatment condition was incubated for 24h at room temperature on shaking incubator (Titer Plate Shaker, Lab-Line Instruments) with 3% donkey serum (Jackson Immunoresearch), 1% Triton-X (ACROS Organics) ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated for 3 hours at room temperature with Cy3-conjugated anti-sheep secondary antibody (Jackson ImmunoResearch, 715-165-147) diluted 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated for 3 hours at room temperature with Cy5-conjugated anti-sheep secondary antibody (Jackson ImmunoResearch, 713-175-147) diluted 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... floating sections were blocked for 1 hour at room temperature in blocking solution (3% normal goat serum (NGS, Jackson ImmunoResearch Laboratories), 0.3% Triton X-100 (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS and immediately transferred to the secondary antibody solution: Cy3 goat anti-mouse (1:500, Jackson ImmunoResearch) for 2 hours at home temperature ...
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mTORC1-dependent SOCE activity regulates synaptic gene expression and muscle response to denervationbioRxiv - Physiology 2024Quote: ... sections were fixed with PFA 4% and then blocked with 3% IgG free BSA and AffiniPure Mouse IgG Fab Fragments (Jackson ImmunoResearch) in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: Sections were washed 3 times and incubated with species appropriate and minimally cross-reactive fluorophore-conjugated secondary antibodies (1:500, Jackson ImmunoResearch) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed once with 200µl of FACS buffer and stained with of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat# 109-117-008) at a 1:100 dilution and live/dead green fluorescent reactive dye (Thermo Fisher Cat# L34970 ...
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bioRxiv - Immunology 2021Quote: ... Plates were developed using 15μl/well of combination of horseradish peroxidase–conjugated antibodies in assay diluent comprising) goat anti-human IgG (Jackson ImmunoResearch Laboratories,109-035-098) at 1:10,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... murine recPrP23-110 and human recPrP121-230 and BSA (coated at 43 nM) in ELISA using goat anti-human IgG Fc-gamma specific-HRP (Jackson ImmunoResearch #109-035-098), 1:4’000 for detection.
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bioRxiv - Microbiology 2020Quote: ... followed by secondary labeling with 1:100 FITC-conjugated anti-Myc to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Immunology 2020Quote: ... 1µg of each mAb was complexed with 3µg of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat #109-117-008), and the labeled mAb was incubated for 30 min at RT prior to dilution to 5µg/mL in Freestyle medium containing 10% FBS and 1% Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/30 000) or goat anti-human IgA (α-chain specific) HRP conjugate (1/10 000) (all from Jackson ImmunoResearch Laboratories, Inc.) were added and samples were incubated at 37°C for 1h ...
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bioRxiv - Immunology 2021Quote: ... for 1 h at room temperature followed by incubation for 1h at room temperature with anti-human peroxidase conjugate IgG (Jackson ImmunoResearch, Ely, Cambridgeshire, UK) or with anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 μl of 5 μg/ml HIV-1 Env gp140 SF162.B (NIH AIDS Reagent Program) or 1 μg/ml anti-human IgG or IgM (Jackson Immunoresearch, West Grove, PA) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... the arrays were washed with T-TBS three times followed by the incubation with goat anti-human IgG+IgA+IgM pAb or donkey-anti-rabbit IgG (HRP conjugates, 1:20,000 dilution; Jackson ImmunoResearch, West Grove, Pennsylvania, USA). After 30 min incubation ...