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Citations for Jackson ImmunoResearch :
451 - 492 of 492 citations for Follicle stimulating hormone FSH Human HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... 1:75,000) against human kisspeptin-54 (aa 68-121 of NP_002247.3) (9, 53) using biotinylated secondary antibodies (Jackson ImmunoResearch Europe, Cambridgeshire, UK; 1:500), the ABC Elite reagent (Vector ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed once with 200µl of FACS buffer and stained with of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat# 109-117-008) at a 1:100 dilution and live/dead green fluorescent reactive dye (Thermo Fisher Cat# L34970 ...
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bioRxiv - Immunology 2021Quote: ... Plates were developed using 15μl/well of combination of horseradish peroxidase–conjugated antibodies in assay diluent comprising) goat anti-human IgG (Jackson ImmunoResearch Laboratories,109-035-098) at 1:10,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... murine recPrP23-110 and human recPrP121-230 and BSA (coated at 43 nM) in ELISA using goat anti-human IgG Fc-gamma specific-HRP (Jackson ImmunoResearch #109-035-098), 1:4’000 for detection.
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bioRxiv - Microbiology 2020Quote: ... followed by secondary labeling with 1:100 FITC-conjugated anti-Myc to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Immunology 2020Quote: ... 1µg of each mAb was complexed with 3µg of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat #109-117-008), and the labeled mAb was incubated for 30 min at RT prior to dilution to 5µg/mL in Freestyle medium containing 10% FBS and 1% Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/30 000) or goat anti-human IgA (α-chain specific) HRP conjugate (1/10 000) (all from Jackson ImmunoResearch Laboratories, Inc.) were added and samples were incubated at 37°C for 1h ...
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bioRxiv - Immunology 2021Quote: ... for 1 h at room temperature followed by incubation for 1h at room temperature with anti-human peroxidase conjugate IgG (Jackson ImmunoResearch, Ely, Cambridgeshire, UK) or with anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 μl of 5 μg/ml HIV-1 Env gp140 SF162.B (NIH AIDS Reagent Program) or 1 μg/ml anti-human IgG or IgM (Jackson Immunoresearch, West Grove, PA) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... the arrays were washed with T-TBS three times followed by the incubation with goat anti-human IgG+IgA+IgM pAb or donkey-anti-rabbit IgG (HRP conjugates, 1:20,000 dilution; Jackson ImmunoResearch, West Grove, Pennsylvania, USA). After 30 min incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a 1 hour incubation with a 1:2000 dilution of anti-human IgG-HRP (#109-035-088, Jackson ImmunoResearch, West Grove, PA) for Her2-Fc detection ...
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bioRxiv - Microbiology 2022Quote: ... incubated with 1:100 FITC-conjugated chicken anti-Myc antibody to label RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label bound antibody ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... an ELISA assay was developed to detect LH01 or un-cleaved LH05 with IL-15Rβ coated on the plate and Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) as the detection antibody ...
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bioRxiv - Immunology 2023Quote: ... containing human IgG1 constant regions were tested at 4µg/ml and at three four-fold dilutions and were detected with peroxidase-conjugated goat anti-human IgG Fc (Jackson ImmunoResearch, 109-035-098). Peroxidase was revealed with 1-Step ABTS substrate solution (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody for flow cytometry we used PE-conjugated goat anti-human IgG + IgM (H+L) (F(ab’)2) (Jackson Immunoresearch, no. 109-116-127).
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and the binding of VHH-Fcs to denatured S was probed by incubating strips with 1 mL of 100 ng/mL anti-human Ig Fc antibody-peroxidase conjugate (Jackson ImmunoResearch, Cat#016-030-084) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... washed twice with PBSB by centrifugation for 5 min at 1200 rpm and then incubated for an additional hour with 50 µL of R-Phycoerythrin AffiniPure F(ab’)₂ Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, Cat#109-116-170) at 250 ng/mL diluted in PBSB ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted washed twice with PBSA and subsequently labeled with PE-conjugated goat anti-human IgG (1:100, Jackson ImmunoResearch Labs #109-115-098) and FITC-conjugated chicken anti-cMmyc (1:100 ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 µL of FACS buffer containing secondary R-PE Goat Anti-Human IgG antibody diluted 1:100 (Jackson ImmunoResearch, cat #109-117-008), and incubated for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Total IgG was measured following 6 washes and a 1-h incubation with 100 μL/well of 1:50,000 HRP-conjugated polyclonal goat anti-human IgG (Jackson ImmunoResearch Labs, West Grove, PA, USA). IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech ...
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bioRxiv - Immunology 2023Quote: ... Grids were washed several times with PBS and incubated with 12-nm gold-labeled anti-human secondary antibody (Jackson ImmunoResearch, diluted 1:40 in PBS) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... wells were washed 6 times and 1:5000 dilution of horseradish peroxidase (HRP)-conjugated donkey anti-human IgG (FC specific; Jackson ImmunoResearch Laboratories, West Grove, PA) was added in blocking solution to each well and the plate was incubated for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-human IgM (Jackson Immunoresearch Affinipure; 0.6-75 ug/mL), or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure ...
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bioRxiv - Cell Biology 2021Quote: External beads were stained for live-cell imaging by incubating macrophages with Cy2-conjugated anti-human IgG (1:100; Jackson ImmunoResearch Labs, West Grove, PA, USA) for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed with PBS and incubated with allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (Jackson ImmunoResearch Laboratories, cat# 709-136-149) at a final concentration of 62.5 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human serum IgA (α-chain specific) at 0.3 μg/mL or goat anti-human IgG (γ-chain specific) at 5 μg/mL (all from Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Microtiter plates were sealed and stored overnight at 2-8°C ...
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bioRxiv - Immunology 2023Quote: ... or unconjugated anti-human CCL19 (polyclonal, R&D AF361-SP, 1:200) that was detected with anti-goat IgG A488 (Jackson Immunoresearch 705-545-003, 1:1000) and unconjugated anti-human PDPN (Clone NZ-1.3 ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were washed twice with filtered PBS and then resuspended in 0.2 µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG (Jackson ImmunoResearch, 1:100, 109-605-098, AB_2337889) and Brilliant Violet 421-anti-rabbit IgG (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation with 1 µg/ml of recombinant sE WT or dimer-mutants or anti-human IgG-HRP (Jackson ImmunoResearch, 109-035-088, 1:5000) in PBS+0.5% FCS for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: Ficolled and FACS-sorted (CD3-/CD14-/CD19+) human peripheral B cells were stimulated with either BCR cross-linking antibodies (αBCR; Jackson Immunoresearch, Cata No. 109-006-129, 10ug/mL) or CD40L (Enzo Life Sciences ...
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bioRxiv - Immunology 2019Quote: ... The expression of GXMR-CAR on the surface of human T cells was analyzed via flow cytometry (MACSQuant; Miltenyi Biotech) using a goat anti-human IgG antibody (Fc-γ fragment-specific, 1:100 dilution; Jackson ImmunoResearch Laboratories; cat. no. 109-606-098). Human T cells were identified using flow cytometry with an anti-human CD3-PE antibody (1:100 dilution ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS-T and were subsequently treated for 30 min with anti-human Alexa 647–conjugated secondary antibody (Jackson ImmunoResearch; dilution: 1:200 in PBS-T + 2 % BSA) to immunostain the centromeres ...