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Citations for Jackson ImmunoResearch :
351 - 400 of 430 citations for 7 bromoquinolin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... at 2 ug/mL and the secondary antibody was Alexa Fluor 647 AffiniPure Donkey Anti-Goat IgG (Jackson Immunoresearch, cat.: 705-605-147) used at 15 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... and HRP-conjugated goat polyclonal anti-human IgG F(ab’)2 fragment antibodies (Jackson ImmunoResearch 109-036-008, 1:5000 dilution in PBSM) were used as secondary detection antibodies for sera and Motavizumab ...
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bioRxiv - Immunology 2022Quote: ... followed by staining with goat-anti-mouse IgG F(ab’)2-HRP (Jackson ImmunoResearch; 1:5000 diluted in TBS-T with 1% BSA). All incubations were 1h at RT and followed by extensive washing with TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and incubated for 2 h at RT with donkey anti-mouse Cy3 or donkey anti-rabbit Alexa 488 secondary antibodies (Jackson Immunoresearch, Suffolk, UK). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch, West Grove, PA). Sections were then mounted on slides (ProLong Gold ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 hours with a secondary anti-rabbit antibody conjugated to Alexa 647 (1:500; Jackson Immunoresearch, 111-605-045) with 5% normal goat serum in PBS + 0.3% Tween-20.
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bioRxiv - Neuroscience 2023Quote: ... and then probed for 2 hours with goat anti-HRP conjugated Alexa Fluor 647 (1:1000, or 0.5 mg/mL, Jackson ImmunoResearch, West Grove, PA). The anti-HRP antibody recognizes a glycoprotein epitope that is present throughout the fruit fly nervous system ...
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bioRxiv - Cell Biology 2023Quote: ... AlexaFluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (cat. #711-546-152, Jackson ImmunoResearch, West Grove, PA, USA); DRAQ5 (cat ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 x 15 min with 0.1 M PBS prior to a 2-hour incubation in secondary antibodies FITC (1:50 in 0.1 M PBS, Jackson ImmunoResearch, 703-095-155), Cy3 (1:50 in antibody diluent ...
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bioRxiv - Pathology 2023Quote: ... and further incubated for 1h at room temperature with cy3 AffiniPure F(ab’)2 fragment rabbit anti-goat IgG and alexa fluor 647 AffiniPure donkey anti-rat IgG secondary antibodies (Jackson ImmunoResearch Europe Ltd). Slides were mounted with Vectashield mounting medium with DAPI (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... and remaining cell surface antibody blocked by incubation with 0.13 mg/ml F(ab’)2 fragment goat-anti rabbit IgG (Jackson Immunoresearch, 111-006-045) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS-T for 5 minutes (3x) and incubated for 2 hours at room with secondary antibody (AlexaFluor-488 1:100, Jackson ImmunoResearch Laboratories, 703545145). Slices were mounted onto slides (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... specimens were rinsed 5 times in PBS and treated for 2 h at room temperature in species-specific secondary antibodies conjugated to either DyLight 405 (1:500, Jackson ImmunoResearch Laboratories Inc.), or AlexaFluor-488 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h at room temperature in a solution containing a biotinylated goat anti-rabbit secondary antibody (Jackson Immunoresearch, 1:2000). Sections were washed again and processed with the avidin-biotin complex for 2 hr at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were revealed using Fab’2 fragments of Donkey anti-rat or goat IgG linked to FITC or TRITC (1:500, Jackson ImmunoResearch, West Grove, USA). Washed sections were coverslipped with antifade medium containing DAPI and images were digitally captured using a Leica DM6000 microscope-coupled coolsnap camera and visualized with Metavue 5.0 software (Molecular Devices ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with the following secondary antibodies diluted in 5% skim milk in TBS-T for 2 h at room temperature: peroxidase-conjugated anti-rabbit IgG antibody (Jackson ImmunoResearch, 711-035-152) at 1:5000 and peroxidase-conjugated anti-mouse IgG (Nacalai Tesque ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). Sections were then washed and processed using 4”,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biophysics 2021Quote: ... Transverse 50 μm sections were obtained using Leica microtome and stained using mouse monoclonal β-tubulin antibody (E7, DSHB; 1-2 μg/ml) followed by secondary goat anti-mouse Cy3 antibody (Jackson ImmunoResearch; 1:300). To visualize actin filaments in embryos microinjected with 0.5 μm nanoparticles ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in biotin-conjugated donkey anti-rabbit secondary antibody (1:1000 in 2% BSA and PBS, #711-065-152, Jackson ImmunoResearch, Cambridgeshire, United Kingdom) for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (Jackson ImmunoResearch Cat# 715-586-151, RRID:AB_2340858) antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (Jackson ImmunoResearch Cat# 711-586-152, RRID:AB_2340622). After several rinses in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Frozen VQ- D2 donor cells were gently thawed at 37°C before being washed twice in PBS containing 2% mouse serum (Jackson ImmunoResearch, #015-000-120). Donor cells were then resuspended in 200 μl of PBS containing 2% mouse serum ...
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bioRxiv - Neuroscience 2022Quote: ... then treated for 2 hours in a PBS solution containing 0.3% TritonX-100 and 10% normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, West Grove, PA). Sections were then treated overnight at 4°C with a PBS solution containing 1% NDS ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed again in PBS-triton and incubated for 2 hours with the secondary antibodies at 1:1000 dilution (Alexa Fluor 488 anti-chicken (Jackson ImmunoResearch 703-545-155) and Alexa Fluor 568 anti-rabbit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Staining with primary antibodies was performed for 24 h (at 4 °C) with subsequently 2 h incubation with secondary antibodies (Jackson ImmunoResearch, West Grove, PA) diluted with 1 % BSA ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were light-protected and incubated at room temperature for 2 h with donkey anti- rabbit IgG AlexaFluor®488-conjugated (1:400; Jackson ImmunoResearch, Pennsylvania, USA) and Streptavidin AlexaFluor®594-conjugated (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-ACK2 primary antibodies were incubated for 2 h in biotinylated secondary antiserum (anti-rabbit 1:2500 or anti-rat, 1:1000; Jackson ImmunoResearch, West Grove, PA) specific to the species of the primary antibodies and were constituted in PBS with 0.32% TX ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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bioRxiv - Cell Biology 2023Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Neuroscience 2024Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). In the concluding steps of the staining protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody for flow cytometry we used PE-conjugated goat anti-human IgG + IgM (H+L) (F(ab’)2) (Jackson Immunoresearch, no. 109-116-127).
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bioRxiv - Biochemistry 2021Quote: ... then probed with H2.3 primary rabbit polyclonal anti-HSulf-2 antibody (dil. 1/1000) followed by fluorescent-conjugated secondary anti-rabbit antibody (Jackson ImmunoResearch Laboratories, dil. 1/500). All the incubations were performed for 2 h at 4 °C in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and then incubated for 2 h at room temperature with Alexa 488 goat anti mouse 1:1000 (Jackson ImmunoResearch, cat n° 115-545-166), Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... BCR-mediated Ca2+ mobilization was measured for 60s after the cells were stimulated either with 10 μg/mL F(ab′)2 of polyclonal goat anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA, USA) or 0.5mM phosphatidylcholine (PtC ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...