Labshake search
Citations for Jackson ImmunoResearch :
551 - 600 of 607 citations for 7 Bromo 5 fluoro 3 methyl 1H indole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
-
bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
-
bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
-
bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
-
bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and Alexa488-conjugated anti-chicken secondary antibodies (Jackson ImmunoResearch, 715-165-150 and 711-225-152) at 1:500 and 1:1000 dilution in a solution of 1.5% BSA in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were washed 3 times with PBS and then incubated with 1:200 Alexa Fluor 488 donkey anti-mouse IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA; 715-545-151), 1:200 Fluorescein (Cy3 ...
-
bioRxiv - Molecular Biology 2024Quote: ... mouse IgG was detected using Cy™3 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Cat# 715-166-150; 1:500 dilution) and rabbit IgG was detected using Cy™5 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
-
bioRxiv - Biophysics 2019Quote: ... plasma membrane-localized BCR was labeled for 10 min at room temperature with 5 mg/mL goat anti-mouse IgM (Jackson ImmunoResearch; RRID: AB_2338477) f(Ab)1 fragments conjugated to both silicon rhodamine (SiR ...
-
bioRxiv - Immunology 2020Quote: ... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
-
bioRxiv - Genetics 2021Quote: ... The fixed tissues were then subjected to three rounds of 15 minute-each wash with 0.1% PBST (0.1% Triton X-100 in PBS) and blocked with 5% donkey serum (Jackson ImmunoResearch Laboratories, 107-000-121) for 1 hour at room temperature followed by incubation with primary antibodies in 5% donkey serum at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: ... Brain sections for immunoprocessing were incubated 60 minutes at room temperature in 5% normal goat serum (NGS, Jackson ImmunoResearch, cat# 005-000-121) to block non-specific labeling and incubated 48 hours at room temperature in a cocktail of primary antibodies and diluent (1% NGS in PBS containing 0.25% Triton X-100 ...
-
bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked in 5% w/v bovine serum albumin (IgG-free and protease-free BSA, Jackson ImmunoResearch Laboratories, West Grove, PA) in 50 mM Tris-HCl (pH 7.4) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 5 minutes each in PBS+0.3% TritonX-100 and incubated with appropriate fluorescent conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 45 minutes at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Antibodies for O1 and O4 were diluted in N2B27 base medium supplemented with 5% Donkey Serum (v/v) (Jackson ImmunoResearch, 017-000-121) and added to wells for 18 minutes at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... washed with PBS-T for 5 minutes (3x) and incubated for 2 hours at room with secondary antibody (AlexaFluor-488 1:100, Jackson ImmunoResearch Laboratories, 703545145). Slices were mounted onto slides (ThermoFisher ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with the following secondary antibodies diluted in 5% skim milk in TBS-T for 2 h at room temperature: peroxidase-conjugated anti-rabbit IgG antibody (Jackson ImmunoResearch, 711-035-152) at 1:5000 and peroxidase-conjugated anti-mouse IgG (Nacalai Tesque ...
-
Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... deparaffinized sections were boiled in 0.1 M Sodium Citrate for 10 minutes for antigen retrieval and sections were blocked in suppressor serum (5% goat serum (Jackson ImmunoResearch #005-000-1210), 95% blocking solution (3% BSA and 0.5% Tween in PBS) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
-
Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat#017-000-121, Jackson ImmunoResearch, West Grove, PA) diluted in 1x PBS (blocking buffer ...
-
bioRxiv - Immunology 2019Quote: ... The cells were then placed on ice and labeled for 5 min with 1 ng/ml Cy3-conjugated anti-mouse IgM Fab fragments (Jackson ImmunoResearch, #115-167-020), 100 ng/ml Cy3-conjugated Fab fragments from the 11-26c rat anti-mouse IgD monoclonal antibody (ATCC ...
-
bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
-
bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
-
bioRxiv - Neuroscience 2023Quote: ... were washed in 1X TBS three times and then blocked and permeabilized for one hour in 1X TBS containing 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, Lot #161590) and 0.5% Triton x-100 ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
-
bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
-
bioRxiv - Developmental Biology 2024Quote: P5-6 retinas were incubated in wash buffer (PBS with 0.1%Triton X100) with gentle rocking for 15 min and then in blocking buffer (wash buffer with 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121)) for 1-2 hrs ...
-
bioRxiv - Immunology 2024Quote: ... or cultured cells stained for the expression of surface markers with specific antibodies in PBS with 5% FBS and 20% normal goat serum (Jackson ImmunoResearch 005-000-121) and 0.01% NaN3 for 20 min on ice ...
-
bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... treated with glycine (100 nM in PBS) and blocked with 5 % skim milk powder or 10 % normal goat serum (Jackson ImmunoResearch, West Grove, Pennsylvania, USA) and 1 % BSA in PBST for 1 h at 37 °C ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked with 5% milk in TTBS before overnight incubation with primary antibody and visualization using HRP-tagged secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) and SuperSignal West Pico PLUS Chemiluminescent substrate (ThermoFisher) ...
-
bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 diluted in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121; Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed twice in 1XPBS for 5’ each and incubated with Goat anti-Rat-Biotin (1:300, in 1XPBS + 1%NGS, Jackson ImmunoResearch Europe Ltd, Ely, UK) at RT for 1h.
-
bioRxiv - Microbiology 2023Quote: ... secondary antibodies were washed three times for 10 mins each in PBS-T and then the slide regions were blocked in 5% normal mouse serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS-T for 1 hour after which NucBlue Fixed Cell ReadyProbes and Alexa 647 conjugated E-cadherin were applied diluted in PBS-T for 1 hour at room temperature with slides kept dark within the humid slide staining tray ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were washed 4 x 5 min in 0.1 M PB then incubated with a biotinylated secondary antibody (biotinylated donkey anti-mouse, Jackson ImmunoResearch, 715-065-151, 1:500) for 1 hour at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... NEG-50 was removed by short incubation in PBS and then sections were blocked and permeabilized by incubation in 5% Normal Donkey Serum (NDS, Jackson ImmunoResearch Cat#017-000-121) in Sorensońs supplemented with 0.5% Triton-X for 1-2 h at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... and rabbit IgG was detected using Cy™5 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, Cat# 711-175-152; 1:500 dilution).
-
bioRxiv - Immunology 2020Quote: ... cells were washed with PBS at 400 × g 5 minutes and then stained with FITC-conjugated AffiniPure donkey or Cy5-conjugated AffiniPure donkey anti-mouse 2nd Abs (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at 1:100 dilution for 30 minutes at room temperature ...