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Citations for Jackson ImmunoResearch :
351 - 386 of 386 citations for 5 tert butoxy 5 oxopentanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat#017-000-121, Jackson ImmunoResearch, West Grove, PA) diluted in 1x PBS (blocking buffer ...
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bioRxiv - Immunology 2019Quote: ... The cells were then placed on ice and labeled for 5 min with 1 ng/ml Cy3-conjugated anti-mouse IgM Fab fragments (Jackson ImmunoResearch, #115-167-020), 100 ng/ml Cy3-conjugated Fab fragments from the 11-26c rat anti-mouse IgD monoclonal antibody (ATCC ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
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bioRxiv - Neuroscience 2023Quote: ... were washed in 1X TBS three times and then blocked and permeabilized for one hour in 1X TBS containing 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, Lot #161590) and 0.5% Triton x-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Developmental Biology 2024Quote: P5-6 retinas were incubated in wash buffer (PBS with 0.1%Triton X100) with gentle rocking for 15 min and then in blocking buffer (wash buffer with 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121)) for 1-2 hrs ...
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bioRxiv - Immunology 2024Quote: ... or cultured cells stained for the expression of surface markers with specific antibodies in PBS with 5% FBS and 20% normal goat serum (Jackson ImmunoResearch 005-000-121) and 0.01% NaN3 for 20 min on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... treated with glycine (100 nM in PBS) and blocked with 5 % skim milk powder or 10 % normal goat serum (Jackson ImmunoResearch, West Grove, Pennsylvania, USA) and 1 % BSA in PBST for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked with 5% milk in TTBS before overnight incubation with primary antibody and visualization using HRP-tagged secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) and SuperSignal West Pico PLUS Chemiluminescent substrate (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 diluted in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121; Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed twice in 1XPBS for 5’ each and incubated with Goat anti-Rat-Biotin (1:300, in 1XPBS + 1%NGS, Jackson ImmunoResearch Europe Ltd, Ely, UK) at RT for 1h.
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bioRxiv - Microbiology 2023Quote: ... secondary antibodies were washed three times for 10 mins each in PBS-T and then the slide regions were blocked in 5% normal mouse serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS-T for 1 hour after which NucBlue Fixed Cell ReadyProbes and Alexa 647 conjugated E-cadherin were applied diluted in PBS-T for 1 hour at room temperature with slides kept dark within the humid slide staining tray ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 4 x 5 min in 0.1 M PB then incubated with a biotinylated secondary antibody (biotinylated donkey anti-mouse, Jackson ImmunoResearch, 715-065-151, 1:500) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... NEG-50 was removed by short incubation in PBS and then sections were blocked and permeabilized by incubation in 5% Normal Donkey Serum (NDS, Jackson ImmunoResearch Cat#017-000-121) in Sorensońs supplemented with 0.5% Triton-X for 1-2 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and rabbit IgG was detected using Cy™5 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, Cat# 711-175-152; 1:500 dilution).
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS at 400 × g 5 minutes and then stained with FITC-conjugated AffiniPure donkey or Cy5-conjugated AffiniPure donkey anti-mouse 2nd Abs (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at 1:100 dilution for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human serum IgA (α-chain specific) at 0.3 μg/mL or goat anti-human IgG (γ-chain specific) at 5 μg/mL (all from Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Microtiter plates were sealed and stored overnight at 2-8°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and blocked with 100 μL blocking buffer (1% bovine serum albumin and 5% normal donkey serum [Jackson ImmunoResearch, cat 017-000-121, lot 152961] in PBST) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 3x for 5 min and incubated at room temperature with blocking buffer containing goat anti-rabbit HRP secondary antibody (Jackson Immunoresearch labs, #111-035-003, 1:5000). Blots were rinsed in wash buffer 3x for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... slides were blocked for 2 h in 1x PBS containing 0.1% Triton X-100 and 5% normal goat or donkey serum (Jackson ImmunoResearch, 005-000-121 and 017-000-121). Sections were incubated with primary antibodies diluted in blocking solution (5% goat or donkey serum with no detergent ...
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bioRxiv - Neuroscience 2022Quote: ... (5) the slices were incubated in the solution of secondary antibodies: CyTM2 AffiniPure donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, Ely, UK catalog number 711-225-152) and CyTM5 AffiniPure donkey anti-chicken IgG (H+L ...
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bioRxiv - Genetics 2023Quote: ... Membranes were washed with 0,1% Tween-20 in PBS (5 times) and incubated for 1 hour at room temperature (RT) with horseradish peroxidase-conjugated secondary antibodies (1:2000, Jackson ImmunoResearch, 515-035-003 and 711-035-152). Membranes were washed again before protein detection ...
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bioRxiv - Neuroscience 2023Quote: ... five times for 5 min and incubated in BB containing biotinylated goat anti-rabbit polyclonal secondary antibody ([1:200] Jackson ImmunoResearch, West Grove, PA, USA, cat# 111-066-046) in TBS for 1.5 h ...
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bioRxiv - Cell Biology 2023Quote: ... S6K1 (amino acids 485-502; rat 70 kDa isoform) (custom-made, as described [73]. Donkey anti-rabbit-HRP (Jackson ImmunoResearch, 711-095-152) was used for detection of ECL-based western blot signals ...