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Citations for Jackson ImmunoResearch :
1 - 50 of 4569 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Neuroscience 2024Quote: ... 2–3 hours in secondary antibody 1:500 anti-chicken-IgY Alexa488-conjugated (Jackson ImmunoResearch cat# 703-545-155) in blocking solution ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with H2.3 primary rabbit polyclonal anti-HSulf-2 antibody (dil. 1/1000) followed by fluorescent-conjugated secondary anti-rabbit antibody (Jackson ImmunoResearch Laboratories, dil. 1/500). All the incubations were performed for 2 h at 4 °C in TBS ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBST 3 times (5 min in each time) and incubated with secondary antibodies (mouse anti-Cy3, Jackson Immunoresearch,1:300) for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... the seedlings were washed for three times 5 min each and then incubated in the secondary antibody Cy-3-goat anti-mouse IgG (1:200; Jackson Immunoresearch, http://www.jacksonimmuno.com). The imaging was performed on a Nikon laser scanning microscope (Eclipse Ti equipped with Nikon C2 Si laser scanning unit ...
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bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 2-3 hours (0.3% Triton X-100, American Bioanalytical, Canton, MA; 3% normal donkey serum, Jackson ImmunoResearch, West Grove, PA), then incubated overnight with primary antibodies (1:1000 + 1% normal donkey serum) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed (3 × 5mins in 0.01M PBS) and then incubated with secondary antibody for 2 hr (Jackson ImmunoResearch, 1:200 in block). Following washing (3 × 5mins in 0.01M PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... Peroxidase-conjuguated AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (1/5 000; Jackson ImmunoResearch; 711-036-152) for anti-Vinculin ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated for 1-2 hrat room temperature with blocking solution of 5% goat serum in PBS (005–000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GTX135357 ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed 3 x 5 min with PBS and the secondary antibodies – donkey-anti-mouse Cy3 (1:500, Jackson ImmunoResearch, 715-165-151) and goat-anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 3×3 minutes in PBS then stained with secondary antibody (Jackson ImmunoResearch, 1:500) diluted in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 3×3 minutes in PBS then stained with secondary antibody (Jackson ImmunoResearch, 1:500) diluted in PBS for 1 hour at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Samples were blocked for at least 3 hours in 5% normal goat serum (NGS; Jackson ImmunoResearch) and 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... or Cy-3 donkey anti-mouse (1:500; Jackson ImmunoResearch) and 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... Cy-3 conjugated donkey anti-mouse (Jackson ImmunoResearch; 1:500) and Alexa Fluor™ 555-conjugated donkey anti-mouse (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were permeabilized with 0.1% Triton X-100 for 3 min and blocked with 5% donkey serum (Jackson ImmunoResearch), followed by incubation with antibodies for 1 hr at room temperature or overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... F(ab’)2 fragment specific (Jackson ImmunoResearch: 1:1000). Cells were washed 3x with PBS + 3% BSA and fluorescence was quantified using a CytoFLEX (Beckman Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 3-5 h with a secondary antibody (Alexa Fluor® 488 anti rabbit; Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch, Cambridge ...
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bioRxiv - Neuroscience 2024Quote: ... The floating sections were washed 3×10 min with 1x PBS and blocked with 5% normal donkey serum (Jackson ImmunoResearch), 2% fish gelatin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-mouse Cy™3 (115-165-003, Jackson ImmunoResearch, 1:300), goat anti-rabbit Alexa Fluor Plus 647 (A32733 ...
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bioRxiv - Neuroscience 2024Quote: ... cyanine 3 (Cy3)-conjugated anti-goat (1:400; Jackson ImmunoResearch, West Grove, PA), cyanine 3 (Cy3)-conjugated anti-guinea pig (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... or donkey anti-rabbit Cyanine Dye 3 (Cy3) (1:300, Jackson ImmunoResearch, UK) in PBS/1% Triton X-100 in darkness for 3 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed 3 times for 5 min in PBS and incubated for 30 min with a cyanine 3-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch) and DAPI (4′,6-diamidino-2-phenylindole).
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... in 1% (w/v) BSA 2% donkey serum (Jackson Immunoresearch, West Grove ...
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bioRxiv - Neuroscience 2020Quote: ... Cy™ 3-conjugated donkey anti-rabbit (1:500; 711-006-152, Jackson ImmunoResearch), Cy™ 5-conjugated donkey anti-mouse (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following secondary antibodies were used: Cy3 anti-mouse IgG (subclasses 1+2a+2b+3) 1:250 (Jackson ImmunoResearch), Alexa Fluor 488 anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating brain sections were blocked in 3% normal donkey serum for 2 h (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then incubated with rabbit anti-DsRed primary antibody (1:1,000 ...
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bioRxiv - Bioengineering 2022Quote: ... or 5-tagged secondary antibodies raised in donkey [1:500 (Jackson ImmunoResearch), at 22–24°C for 2 h] ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 5 mins with 2% (vol/vol) normal mouse and normal rat serum (Jackson ImmunoResearch Laboratories), prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse unconjugated F(ab’)2 fragments (1:300, Jackson ImmunoResearch), were added in the blocking step ...
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-mouse IgG (H+L) (Cy 3; 715-165-151, Jackson Immunoresearch; 1:800), and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in PBS (5 x 5 min) and incubated in donkey anti-rabbit Cy5 secondary (1:1000 in PBS, Jackson ImmunoResearch, AB_2340607) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... cyanine 5(Cy5)- and/or FITC-conjugated secondary antibodies (1:400; Jackson ImmunoResearch) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed for 1 hour blocking in 5% Normal Donkey Serum (NDS, Jackson ImmunoResearch) and 0.05% Triton X-100 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1: 10 000 in 5% milk/TBSP (Jackson ImmunoResearch, West Grove, PA, USA). HA-tagged proteins were detected using a Westar Supernova kit (Cyanagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and Cy 3-Conjugated AffiniPure Goat Anti-Mouse IgG (Jackson ImmunoResearch, #115-165-146, 1:1000).