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Citations for Jackson ImmunoResearch :
1 - 50 of 4631 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Slides were incubated at 4°C with primary (Day 1) and secondary (Day 2) antibodies (Jackson ImmunoResearch) overnight in PBT and 5% donkey serum in a humidified chamber and washed three times for 10 min after each antibody incubation with PBT ...
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bioRxiv - Neuroscience 2021Quote: ... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...
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bioRxiv - Neuroscience 2024Quote: ... 2–3 hours in secondary antibody 1:500 anti-chicken-IgY Alexa488-conjugated (Jackson ImmunoResearch cat# 703-545-155) in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... blocking solution consisting of 2 % BSA (Roth) and 4 % donkey serum (Jackson ImmunoResearch) in PBS was added for 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 mM EDTA) and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003 ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Biochemistry 2021Quote: ... F(ab’)2 fragment specific (Jackson ImmunoResearch: 1:1000). Cells were washed 3x with PBS + 3% BSA and fluorescence was quantified using a CytoFLEX (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with H2.3 primary rabbit polyclonal anti-HSulf-2 antibody (dil. 1/1000) followed by fluorescent-conjugated secondary anti-rabbit antibody (Jackson ImmunoResearch Laboratories, dil. 1/500). All the incubations were performed for 2 h at 4 °C in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2–4 h with a mixture of fluorophore-conjugated secondary antibodies (all raised in donkey, 1:500, Jackson Immunoresearch): Anti-guinea pig IgG (DyLight 488 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 days at 4°C (iii) 1:400 of biotinylated anti-goat IgG (Jackson ImmunoResearch, catalog 705-065-147), 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated for 1-2 hrat room temperature with blocking solution of 5% goat serum in PBS (005–000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GTX135357 ...
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bioRxiv - Neuroscience 2023Quote: ... Peroxidase-conjuguated AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (1/5 000; Jackson ImmunoResearch; 711-036-152) for anti-Vinculin ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 2-3 hours (0.3% Triton X-100, American Bioanalytical, Canton, MA; 3% normal donkey serum, Jackson ImmunoResearch, West Grove, PA), then incubated overnight with primary antibodies (1:1000 + 1% normal donkey serum) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed (3 × 5mins in 0.01M PBS) and then incubated with secondary antibody for 2 hr (Jackson ImmunoResearch, 1:200 in block). Following washing (3 × 5mins in 0.01M PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... in 1% (w/v) BSA 2% donkey serum (Jackson Immunoresearch, West Grove ...
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bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or 2 μg/ml anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch), or 5 μg/ml anti-mouse CD40 (clone FGK4.5/ FGK45 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse unconjugated F(ab’)2 fragments (1:300, Jackson ImmunoResearch), were added in the blocking step ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and incubated for 2 hr with F(ab)2 donkey anti-rat IgG-Cy3 antibody (1:200; REF-712-166-153, Jackson ImmunoResearch Laboratories, USA). Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... blocking solution consisting of 2% BSA (w/v) (Roth) and 4% donkey serum (v/v) (Jackson ImmunoResearch Laboratories) in PBS was added for 30 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were permeabilized with 4% Triton X-100 with 2% normal goat serum (Jackson ImmunoResearch #005-000-121) in PBS at 4°C for 2 days ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were permeabilized with 4% Triton X-100 with 2% normal goat serum (Jackson ImmunoResearch #005-000-121) in PBS at 4°C for 2 days ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Immunology 2024Quote: ... F(ab’)2 (Jackson ImmunoResearch) and PE-conjugated streptavidin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary:secondary molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 5 mins with 2% (vol/vol) normal mouse and normal rat serum (Jackson ImmunoResearch Laboratories), prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating brain sections were blocked in 3% normal donkey serum for 2 h (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then incubated with rabbit anti-DsRed primary antibody (1:1,000 ...
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bioRxiv - Plant Biology 2024Quote: PIN1 localization in embryos transgenic for 35Sp:EGFP-ENP used PIN1 primary rabbit antibody incubation (1:1000; 4h, 37°C) and secondary rabbit Cy3-Antibodies (BSA/PBS for 3.5h at 37°C; Jackson ImmunoResearch/USA supplied by Dianova/Hamburg). After repeated washes with PBS and H20 the embryos were embedded in Citifluor antifadent mounting medium and covered with a coverslip ...
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bioRxiv - Immunology 2021Quote: ... anti-IgM F(ab’)2 (1 ug/ml, Jackson Immunoresearch, Cat#115-006-020), IL-21 (50 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary : secondary molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2020Quote: ... then with anti-mouse horseradish peroxidase-conjugated antibodies (1/3000; 2 h; Jackson ImmunoResearch) and revealed with ECL substrate (Lumi-Light Western Blotting Substrate ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 20µL of FACS buffer containing 1-2% normal mouse serum (Jackson ImmunoResearch) and 1-2% mouse gamma globulin ...
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bioRxiv - Immunology 2024Quote: ... 1:1000 diluted Alkaline phosphatase conjugated anti-human F(ab’)2 antibodies (Jackson ImmunoResearch) were incubated in the plate for 1 hr and then washed ...
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bioRxiv - Immunology 2023Quote: ... serum and 1% AffiniPure F(ab′)2 fragment donkey anti-mouse IgG (Jackson ImmunoResearch). Primary antibodies were diluted in IF buffer at the dilutions detailed below (Supplementary table 5 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by fluorescently conjugated secondary antibodies in 1:2 (primary:secondary) molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2024Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary: secondary molar ratio (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2024Quote: ... and then by fluorescently conjugated secondaries in 1:2 primary:secondary molar ratios (Jackson ImmunoResearch). Following active labeling ...
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bioRxiv - Neuroscience 2024Quote: ... 2% v/v normal goat serum (Jackson ImmunoResearch, 005-000-121, 1:500 dilution), and 0.1% w/v Triton X-100 in 1×PBS at 4 °C for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: Tissue was blocked for an hour at room temperature or overnight at 4°C in phosphate buffered saline with 2% Normal Donkey Serum (Jackson ImmunoResearch), followed by 2 hours at room temperature in primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Supplementary Table 2) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Developmental Biology 2021Quote: ... and St18 (1:1000) were incubated in 5% NDS/PBS overnight at 4°C and secondary antibodies (Jackson Immunoresearch, 1:1000) diluted in 0.01% Triton X-100/PBS for 2 hours at room temperature ...