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Citations for Jackson ImmunoResearch :
401 - 450 of 4221 citations for trans 2 2 4 Methoxyphenyl 2 oxoethyl cyclohexane 1 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... AlexaFluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (cat. #711-546-152, Jackson ImmunoResearch, West Grove, PA, USA); DRAQ5 (cat ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Pathology 2023Quote: ... and further incubated for 1h at room temperature with cy3 AffiniPure F(ab’)2 fragment rabbit anti-goat IgG and alexa fluor 647 AffiniPure donkey anti-rat IgG secondary antibodies (Jackson ImmunoResearch Europe Ltd). Slides were mounted with Vectashield mounting medium with DAPI (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... and remaining cell surface antibody blocked by incubation with 0.13 mg/ml F(ab’)2 fragment goat-anti rabbit IgG (Jackson Immunoresearch, 111-006-045) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and incubated for 2 h at RT with donkey anti-mouse Cy3 or donkey anti-rabbit Alexa 488 secondary antibodies (Jackson Immunoresearch, Suffolk, UK). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibody was AffiniPure F(ab’)2 donkey anti-rabbit or donkey anti-mouse IgG (H+L) (711-006-152 and 715-007-003, Jackson ImmunoResearch Europe Ltd) conjugated to the organic fluorophore CF647 (MX647S100 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). Sections were then washed and processed using 4”,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). In the concluding steps of the staining protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (Jackson ImmunoResearch Cat# 715-586-151, RRID:AB_2340858) antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (Jackson ImmunoResearch Cat# 711-586-152, RRID:AB_2340622). After several rinses in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Frozen VQ- D2 donor cells were gently thawed at 37°C before being washed twice in PBS containing 2% mouse serum (Jackson ImmunoResearch, #015-000-120). Donor cells were then resuspended in 200 μl of PBS containing 2% mouse serum ...
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bioRxiv - Neuroscience 2022Quote: ... then treated for 2 hours in a PBS solution containing 0.3% TritonX-100 and 10% normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, West Grove, PA). Sections were then treated overnight at 4°C with a PBS solution containing 1% NDS ...
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... yeast were washed in PBS/0.2% BSA and rotated at 18 rpm in 300 μL of same buffer containing 2 μg/mL Alexa488-conjugated goat anti-chicken IgG (Jackson ImmunoResearch, West Grove, PA) for one hour at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Immunology 2020Quote: ... BCR-mediated Ca2+ mobilization was measured for 60s after the cells were stimulated either with 10 μg/mL F(ab′)2 of polyclonal goat anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA, USA) or 0.5mM phosphatidylcholine (PtC ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h at room temperature with a secondary antibody conjugated to 6-nm gold particles (Jackson ImmunoResearch, cat # 115-195-146) diluted to 1:40 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of red-phycoerythrin (R-PE) conjugated F(ab’)2 fragment goat anti-mouse IgG specific to the Fc fragment (Jackson ImmunoResearch, Inc., West Grove, PA) were added at 1 μg/ml to the wells and incubated for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using goat-anti-human IgG-Alexa fluor-488 or anti-human IgG F(ab’)2-Alexa fluor-488 (Jackson ImmunoResearch, West Grove, PA, USA), and mean cellular fluorescence was determined using flow cytometry (Intellicyt iQue ...
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bioRxiv - Cancer Biology 2023Quote: ... washed thrice with PBS and then stained with goat anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 647 (Jackson ImmunoResearch Labs; West Grove, PA) to monitor for p-STAT-5 in CD8+ OT-I cells by flow cytometry.
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat#: 715-116-150) using flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Immunology 2023Quote: ... or 10 μg/ml AF-488/−647 labelled F(ab’)2 Fragments of donkey anti-mouse IgM (Jackson ImmunoResearch 715-546-020/715-606-020), were placed in 300 μl of imaging buffer (10% FCS in PBS ...
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bioRxiv - Immunology 2024Quote: ... 5% CO2 with 25 µl of secondary antibody alkaline phosphatase conjugated F(ab’)2 goat anti-mouse IgG Fc-gamma specific (Jackson Immunoresearch, USA, # 115-056-071) diluted 1/3000 in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... and MYC-tag was detected with 100 µl per well of HRP-F(ab)2 fragment goat anti-mouse IgG (H+L) (Jackson ImmunoResearch, Cat #115-036-062) at 1/ 5000 in the same binding solution ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunohistochemistry for dsRed was performed with a 7 minute −20°C 100% acetone target retrieval and blocking in 2% normal goat serum (Jackson ImmunoResearch, cat. no. 005-000-121). Primary antibodies include rabbit anti-mCherry (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 3X with FACS buffer and stained with secondary antibody Cy3-conjugated Goat Anti-mouse IgG (Jackson ImmunoResearch, 2 μg/mL in FACS buffer) for 1 hour at RT ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed with PBS and incubated with allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (Jackson ImmunoResearch Laboratories, cat# 709-136-149) at a final concentration of 62.5 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... slides were blocked for 2 h in 1x PBS containing 0.1% Triton X-100 and 5% normal goat or donkey serum (Jackson ImmunoResearch, 005-000-121 and 017-000-121). Sections were incubated with primary antibodies diluted in blocking solution (5% goat or donkey serum with no detergent ...
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bioRxiv - Neuroscience 2024Quote: ... we washed sections six times (5 min each) and then incubated them for 2 hours in biotinylated donkey anti-goat secondary antiserum (Jackson ImmunoResearch, Product#: 705-065-147, Lot#: 129472) diluted 1:500 in PBT with NHS ...
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bioRxiv - Neuroscience 2024Quote: ... we washed sections six times (5 min each) and incubated them for 2 hours in donkey anti-chicken-Alexa Flour 488 antiserum (Jackson ImmunoResearch, Product#: 703-545-155, Lot#: 154923) and biotinylated donkey anti-rat secondary antiserum (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2021Quote: ... Both primary and Alexa Fluor®-conjugated secondary antibodies (Thermo Fisher Scientific and Alexa Fluor® 647 donkey-anti-chicken [#703-606-155] from Jackson Immunoresearch; all at 2 μg/ml final concentration) were diluted in blocking buffer and samples were incubated for 48 hours on a rocking platform at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... (4) incubated in secondary antibody (1:500 703-545-155, Jackson ImmunoResearch) and lectin (for labeling blood vessels ...
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bioRxiv - Biochemistry 2021Quote: ... then with secondary AlexA 488-conjugated antibody (Jackson ImmunoResearch Laboratories, dil. 1/500, 1 h at 4°C). FACS analysis of cell fluorescence was performed on a MACSQuant Analyzer (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked for 1 hour at 4°C in normal donkey serum (1:20; Jackson Immunoresearch; RRID: AB_2337258). Primary antibodies used were chicken anti-GFP (1:1000 ...
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bioRxiv - Physiology 2020Quote: ... A 4-hour incubation of 1:250 secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) followed ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2021Quote: ... primary antibodies were incubated overnight at 4°C followed by HRP-conjugated secondary antibodies for 1 hour at room temperature (1:10000, Jackson ImmunoResearch). Proteins were visualized with chemiluminescent ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Developmental Biology 2021Quote: ... and St18 (1:1000) were incubated in 5% NDS/PBS overnight at 4°C and secondary antibodies (Jackson Immunoresearch, 1:1000) diluted in 0.01% Triton X-100/PBS for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... overnight at 4°C followed by Alexa Fluor 594-conjugated secondary antibody (1:500, Jackson ImmunoResearch) for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...