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Citations for Jackson ImmunoResearch :
401 - 450 of 570 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... plates were incubated at 37°C with Peroxydase Affinipure rabbit anti Human IgG (Jackson ImmunoResearch 309-035-008, 1/1000) in PTG for 45 min and treated with ortho-phenylenediamine solution containing 0.1% hydrogen peroxid (H2O2 ...
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bioRxiv - Immunology 2024Quote: ... Fixed and permeabilized cells were then washed 3x with PBS, and blocked (PBS, 0.3% Triton X-100, and 5% normal human serum (Jackson ImmunoResearch)) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... or with BSA-AF488 (80 µg/mL) mixed with the Fc fragment of human IgG (25 µg/mL; Jackson ImmunoResearch) as a control ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with 2 μg/mL goat anti-human IgG (H+L) Alexa647 (Jackson ImmunoResearch, Cat# 109-605-003) for 30 min on ice ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated with the secondary antibody R-Phycoerythrin AffiniPure Goat Anti-Human IgG (Jackson Immunoresearch 109-115-098). The data was acquired on FACSCanto II ...
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bioRxiv - Biophysics 2020Quote: ... AF647-conjugated anti-mouse-IgM Fab fragments (catalogue #115-607-020) and AF647-conjugated anti-human-IgM Fab fragments (catalogue #109-607-043) were from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Immunology 2021Quote: ... of 0.12 μg/mL was based on the linear range of a sigmoidal standard curve of human IgM (Jackson ImmunoResearch Laboratories). Magnitude of ZIKV-binding IgG was assessed by virion-binding ELISA and neutralization potency was assessed using the Focus Reduction Neutralization Test ...
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bioRxiv - Microbiology 2021Quote: ... The remaining biotinylated human ACE2 binding to the RBD coating on the plate was detected using HRP-labelled strepavidin (016-030-084, Jackson Immunoresearch). The plate were developed with TMB (Tetramethylbenzidine,Biopanda TMB-S-001) ...
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bioRxiv - Biochemistry 2020Quote: ... Detection reagents consisted of (i) goat anti-human IgG (Fcγ specific) F(ab’)2 AF594 conjugate (Jackson ImmunoResearch 109-586-170), (ii ...
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bioRxiv - Pathology 2021Quote: ... followed by FACS sorting using 3.75 μg/ml Alexa Fluor 647–conjugated goat anti-human IgG (Jackson ImmunoResearch, 109-606-170), 5 μg/ml Alexa Fluor 647–conjugated goat anti-human IgM (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... the beads were washed three times in PBT and labelled for 30 min with R-Phycoerythrin-conjugated Goat-anti-Human IgG Fc (Jackson Immunoresearch, 30µl of a 1:600 dilution of stock in assay buffer) ...
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bioRxiv - Immunology 2022Quote: ... then 50 µL of horseradish peroxidase-conjugated secondary Goat Anti-Human secondary IgG antibody (Cat No: 109-035-008, Jackson ImmunoResearch) at 1:40,000 dilution in 3% milk was added for 1 hour at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... Alexa Fluor 647 goat anti-rabbit IgG and Fcγ-specific goat anti-human IgG secondary antibodies were purchased from Jackson Immunoresearch (Jackson ImmunoResearch Labs Cat# 111-005-144 ...
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bioRxiv - Microbiology 2020Quote: ... and IgG responses were detected with goat anti-human IgG conjugated with horseradish peroxidase (anti-IgG F[ab]2 for F[ab]) (Jackson ImmunoResearch). IgM and IgA anti-OSP reactivity of plasma was measured using goat anti-human IgM and IgA conjugated with horseradish peroxidase (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2020Quote: ... IgM and IgA anti-OSP reactivity of plasma was measured using goat anti-human IgM and IgA conjugated with horseradish peroxidase (Jackson ImmunoResearch). Peroxidase activity was measured with the substrate 2,2-azinobis (ethylbenzthiazolinesulfonic acid ...
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bioRxiv - Immunology 2020Quote: ... Bound rhesus macaque or human anti-S1 antibodies present in the serum were detected with a fluorescently labelled goat anti-human polyclonal secondary antibody (Jackson ImmunoResearch). Data were captured as median fluorescent intensities (MFIs ...
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bioRxiv - Immunology 2020Quote: ... cell-bound antibodies were detected using an AffiniPure Donkey anti-human IgG-Alexa Fluor 647 antibody (Jackson Immunoresearch; #709-605-149), which was incubated with the cells for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Bound rhesus macaque or human anti-S1 antibodies present in the serum were detected with a fluorescently labelled goat anti-human polyclonal secondary antibody (Jackson ImmunoResearch). Data were captured as median fluorescent intensities (MFIs ...
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bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa-647- or DyLight-405-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 or 109-475-064 ...
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bioRxiv - Immunology 2020Quote: ... Plates were blocked for 1 hour with PBS/2% BSA and incubated with biotinylated goat anti–human IgG antibodies for 1 hour (5 μg/mL; catalog 109-066-170, Jackson ImmunoResearch). Serum samples were serially diluted and incubated for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the arrays were washed three times with T-TBS and then incubated for 45 min at RT with biotin-conjugated anti-human IgG secondary antibody (Jackson ImmunoResearch), diluted at 1/400 in array blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... Allophycocyanin-conjugated F(ab′)2 goat anti-mouse or donkey anti-human IgG (H+L) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Immunology 2021Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2020Quote: ... Soluble ICAM1-Fc-F(ab’)2 complexes were generated by diluting APC-labeled goat anti-human IgG F(ab’)2 fragments (109-135-098, Jackson Immunoresearch) 1:6.25 with ICAM1-Fc (200μg/ml final ...
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bioRxiv - Immunology 2020Quote: ... Bound SARS-CoV-2 IgG antibodies were then detected by incubating for 30 min at 37°C with a secondary AF488-conjugated AffiniPure goat anti-human IgG antibody (Jackson Immunoresearch), diluted 1:200 in 0.1% Evan’s Blue ...
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bioRxiv - Immunology 2021Quote: ... Either soluble ICAM-1-Fc-F(ab’)2 or VCAM-1-Fc-F(ab’)2 complexes were generated by diluting APC-labelled goat anti-human IgG F(ab’)2 fragments (109-135-098, Jackson Immunoresearch) 1:6.25 with either ICAM-1-Fc (200µg/ml final ...
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bioRxiv - Immunology 2021Quote: ... Coverslips were rinsed and directly immunostained with anti-Env 2G12 or PGT145 bNAbs and AlexaFluor 647 Goat anti-human IgG secondary Ab (Jackson ImmunoResearch) prior to fixation ...
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bioRxiv - Immunology 2021Quote: ... and incubated for four hours in the dark at room temperature with 1ug/ml goat anti-human IgG horse radish peroxidase antibody (Jackson Immunoresearch). Cells were again washed three times with PBS-Tween 20 (0.5% ...
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bioRxiv - Immunology 2020Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2021Quote: ... and recombinant mouse IL21R Fc Chimera (600x; R&D) followed by PE-labeled affinity-purified F(ab’)2 fragment of goat anti-human Fcγ (Jackson ImmunoResearch). For detection of phosphorylated antigens ...
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bioRxiv - Microbiology 2021Quote: ... Membranes overlaid with rH3-Fc were subsequently probed with biotin-SP-conjugated AffiniPure goat anti-human IgG (Jackson ImmunoResearch; 1:2000). Immunodetection was performed using infrared dye-labeled secondary antibodies (IRDye 800CW Biosciences ...
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bioRxiv - Bioengineering 2020Quote: ... donkey anti-human IgG–HRP conjugate (709-035-149) and goat anti-mouse IgG-HRP conjugate (115-035-071) from Jackson ImmunoResearch; mouse anti-human ErbB-2 (CD340 ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed six times and total IgG binding was measured in a 1-h incubation with 100 μL/well of 1:50,000 HRP-conjugated polyclonal goat anti-human IgG (Jackson ImmunoResearch Labs) and developed using 50 μL/well TMB substrate (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Bound ICs were detected using a PE-conjugated goat anti-human IgG F(ab’)2 fragment at 0.5 μg/ml (Jackson ImmunoResearch Laboratories) on a BD FACSCanto II flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... The plates were then washed three times with PBS containing 0.05% Tween 20 and incubated for 1 hr with a dilution of a 1/1,000 horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (Jackson ImmunoResearch Laboratories), developed with TMB Peroxidase Substrate (SeraCare) ...
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bioRxiv - Immunology 2023Quote: ... Binding of soluble ICAM1-Fc-F(ab’)2 complexes were generated by diluting APC-labeled goat anti-human IgG F(ab’)2 fragments (109-135-098, Jackson Immunoresearch) 1:6.25 with rmICAM1-Fc (200µg/ml final ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with PBS containing 3% normal donkey serum and 0.3% Triton X-100 and then incubated with 100 μg/ml unconjugated anti-human IgG Fab fragments (H+L, Jackson Immunoresearch). Slides were then incubated with FM or HC serum diluted 1:500 in PBS with 1% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-mouse TRITC and donkey anti-human Cy5 (1:300, 711-095-152, 111-025-006, 715-025-150, 715-095-150, 709-175-149, Jackson Immunoresearch). Coverslips were mounted on glass slides using Vectashield® anti-fade mounting medium with DAPI staining.
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bioRxiv - Immunology 2023Quote: ... Purified human naïve B cells were cultured in 96-well plates in the presence of AffiniPure F(ab’)₂ Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109-006-127, Jackson ImmunoResearch), anti-human CD40 (5 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Plates were then washed twice with Superwash and the corresponding IgG-HRP secondary antibody (goat anti mouse IgG-HRP or goat anti human IgG-HRP; Jackson Immunoresearch) diluted in SuperBlock (without Sodium azide ...
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bioRxiv - Immunology 2024Quote: ... Presence of IgM was detected using a goat anti-human IgM (Fc5u specific) secondary antibody conjugated to alkaline phosphatase (Jackson ImmunoResearch) (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and then incubated for 1 hr in blocking buffer without NaN3 to which of horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-human IgG Fc specific antibody (Jackson Immunoresearch) had been added at the dilution as recommended by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... washed and incubated for another 30 min at 4°C with APC-labelled donkey-anti-human-IgG (Jackson ImmunoResearch Europe Ltd.). For complement deposition assays ...
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bioRxiv - Immunology 2023Quote: ... All plasma samples were heat-inactivated before usage to minimize risk of residual virus in serum and then incubated at serial dilution followed by multiple washes and incubation with horseradish peroxidase-conjugated secondary Goat Anti-Human secondary IgG (Cat No: 109-035-008, Jackson ImmunoResearch) at 1:40,000 dilution in 3% milk at 37°C for 1 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then incubated with horseradish peroxidase (HRPO) conjugated donkey anti-human IgG (Fcγ fragment specific) antibody (Jackson ImmunoResearch Inc., West Grove, PA), and developed using 3,3’,5,5’-tetramethylbenzidine (TMB/ Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences) and sorted for high and low CD20 expressing cells using rituximab and a BV421-labeled anti-human secondary (Jackson ImmunoResearch). Lentiviral vector encoding HER2/neu was a gift from Mien-Chie Hung (Addgene plasmid #16257) ...
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bioRxiv - Microbiology 2024Quote: ... except that primary antibodies were serially diluted 5-fold and followed by incubation with a 1:3000 dilution of HRP-labeled goat anti-human IgG (Jackson Immunoresearch) in casein for 45 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Conjugate bound to NA or anti-Fc antibody on the plates was then probed with an HRP-conjugated anti-human IgG Fc F(ab’)2 (Jackson ImmunoResearch) diluted 1:2,000 in sample diluent for 1 h at RT with shaking ...
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Inhibition of CELA1 Improves Septation in the Mouse Hyperoxia Model of Impaired Alveolar DevelopmentbioRxiv - Developmental Biology 2024Quote: ... and 35 μg of KF4 in PBS at a concentration of 1 mg/mL or mouse anti-Human IgG (Jackson Immunoresearch) subcutaneously between the shoulder blades based on a dose of 5 mg/kg (5 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and incubated with 1:200 PE-conjugated goat anti-human-IgG antibody (Jackson ImmunoResearch; clone 109-115-098), and 1:100 FITC-conjugated chicken anti-Myc-tag (Immunology Consultants Lab ...