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Citations for Jackson ImmunoResearch :
401 - 450 of 606 citations for 4 1H Benzoimidazol 2 ylsulfanyl butyric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... AffiniPure F(ab’)2-goat anti-rat IgG (H+L) Alexa 488 (Jackson ImmunoResearch Labs Cat# 112-546-003, RRID:AB_2338364), F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109 ...
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bioRxiv - Bioengineering 2023Quote: ... and a 1000x dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson Immunoresearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Cell Biology 2023Quote: ... or allophycocyanin-conjugated (APC) AffiniPure F (ab’)2 fragment goat anti-Rat IgM (1:100, Jackson ImmunoResearch, 112-136-075) at 4°C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Immunology 2023Quote: ... the TCR was stimulated by addition of 15 μg/ml of the CD3 antibody clone UCHT1 or alternatively by restimulation with CD3 mAbs (TR66 clone) and crosslinking by 1.3 μg/mL anti-mouse IgGF(ab)’2 fragments (Jackson ImmunoResearch). The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: ... MilliporeSigma) was applied for 2 h at RT followed by secondary antibody (fluorophore-conjugated IgG, 1:500; Jackson ImmunoResearch Laboratories) for 1 h at RT ...
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bioRxiv - Biophysics 2023Quote: ... permeabilization and and blocking were performed with PBS (with 0.4% (v/v) Triton X-100%) and 2% immunoglobulin G-free BSA (Jackson ImmunoResearch) for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... and 1:1000 dilution of goat anti-human Fc F(‘ab)2 Alexa Fluor 488 (Jackson ImmunoResearch, 109-546-170) on ice for 4 min ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with either 2 µg/ml of donkey anti-mouse IgG (Affinipure; Jackson ImmunoResearch Laboratories, West Grove, PA), carbonate buffer (0.0875 M Na2CO3 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed and incubated for 2 h with FITC-conjugated donkey anti-rabbit antibody (1:150; RRID:AB_2315776, Jackson ImmunoResearch, US) in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was again rinsed three times in 0.1M NaPb for 10 min followed by 2-h in biotin-SP-conjugated donkey anti-rabbit (1:300; Jackson ImmunoResearch) secondary antibody and AlexaFluor-488 donkey anti-chicken secondary antibody (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-mouse conjugated with HRP (1:5,000, 2 hours at room temperature, catalog no. 111-035-146; Jackson ImmunoResearch), goat anti-rat conjugated with HRP (1:5,000 ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-rat conjugated with HRP (1:5,000, 2 hours at room temperature, catalog no. 115-035-143; Jackson ImmunoResearch). Western blots were developed with Thermo Scientific SuperSignal West Pico and Femto PLUS Chemiluminescent Substrate ...
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bioRxiv - Immunology 2024Quote: ... B cells were stimulated with 10 μg/mL polyclonal F(ab’)2 goat anti-mouse IgM (anti-μ) (Jackson ImmunoResearch) or 5 μg/mL anti-IgDa (clone AMS 9.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with 2 μg/mL goat anti-human IgG (H+L) Alexa647 (Jackson ImmunoResearch, Cat# 109-605-003) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... After extensive washes with 2× SSC at 37°C the probe was revealed using Cy-conjugated streptavidin (Jackson Immunoresearch Laboratories), followed by immunostaining as described above.
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti-guinea pig Alexa Fluor (AF) 488 (Catalog #:706-545-148) had a 1:500 dilution and were stored overnight at 4 °C (Jackson ImmunoResearch; West Grove, PA, USA). Sections were then mounted and cover-slipped with using 50% glycerol in 0.02M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Immunology 2020Quote: ... cells were stimulated with a suboptimal concentration of pre-mixed complexes composed of 0.056 µg mL-1 biotinylated F(ab’)2 goat anti-mouse IgM (µ-chain specific) (Jackson ImmunoResearch), ∼3 µg mL-1 C3dg-biotin (produced in house ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with secondary antibodies R-Phycoerythrin AffiniPure F (ab’) 2 Fragment Donkey Anti-Rat IgG (1:300; 712-116-153; Jackson ImmunoResearch) or Alexa Fluor 568 donkey anti-rabbit IgG (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in a secondary antibody solution at room temperature for 2 h with protection from light (1:500, Alexa Fluor 647 donkey anti-chicken, Jackson Immunoresearch, USA ...
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bioRxiv - Neuroscience 2021Quote: ... tissue is washed 10 times in 1X PBS for 1 min each and then incubated for 2 hours in blocking buffer + secondary antibodies from Jackson ImmunoResearch Laboratories (donkey anti-rabbit 488 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for ∼ 2 h at room temperature with the appropriate AlexaFluor or Dylight 488/568/647 conjugated secondary antibodies (ThermoFisher / Jackson ImmunoResearch) and counterstained with DAPI ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and Peroxidase-AffiniPure F(ab’)2 Fragment Goat anti-mouse IgG (111-036-047, Jackson ImmunoResearch, USA) diluted to 1:20,000 in blocking buffer was added to every well and incubated for 1 hr using a plate shaker set to 450 rpm at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated for 1 hour at room temperature with HRP conjugated-(Fab)2 Goat antimouse IgG (Fc specific) or Goat anti-mouse IgM (both 1/10000, Jackson Immunoresearch). Then ...
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bioRxiv - Biochemistry 2020Quote: ... Detection reagents consisted of (i) goat anti-human IgG (Fcγ specific) F(ab’)2 AF594 conjugate (Jackson ImmunoResearch 109-586-170), (ii ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were incubated with F(ab’)2 goat-anti-mouse IgM+G (10 μg/ml, Jackson ImmunoResearch Laboratories) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (Thermo Fisher Scientific or Jackson Immunoresearch). Nuclei were labeled with DAPI ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed at indicated time points in 2% paraformaldehydestained with fluorescently conjugated antibodie Fab fragments targeting IgM (Jackson Immunoresearch) and analysed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... We used the following secondary antibodies: F(ab’)2 conjugates with FITC Donkey Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch 706-096-148 ...
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bioRxiv - Biochemistry 2022Quote: ... The horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and phycoerythrin (PE)-anti-rabbit F(ab’)2 antibody was obtained from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2022Quote: Flat-bottom plate were coated with 20 μg/ml AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L)mouse (Jackson Immunoresearch) in PBS for two hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and IgG responses were detected with goat anti-human IgG conjugated with horseradish peroxidase (anti-IgG F[ab]2 for F[ab]) (Jackson ImmunoResearch). IgM and IgA anti-OSP reactivity of plasma was measured using goat anti-human IgM and IgA conjugated with horseradish peroxidase (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2020Quote: ... Purity of the F[ab] fragments was assessed by Western blot analysis with 100 ng of sample per well and direct probing with anti-IgG F[ab]2 and anti-IgG Fc conjugated to horseradish peroxidase (Jackson ImmunoResearch).
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed 3 × 5 minutes in PBS prior to a 2 hour incubation with a biotin-conjugated donkey anti-rat secondary antibody (1:500, 712-065-153, Jackson ImmunoResearch). The primary–secondary antibody complexes were detected via peroxidase-driven precipitation of diaminobenzidine (DAB) ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2021Quote: ... RRID: AB_2532109) in PBS with 0.5% Triton X-100 (MilliporeSigma) and 2% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA). Sections were then washed in PBS and incubated for 90 min at room temperature in Alexa Fluor 488- or 594-conjugated donkey anti-mouse IgG (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... Several rinsing with TBS was followed by incubation in secondary antibody solution for 2 hours (1:500 Cy3 conjugated goat anti-rabbit IgG, #134845, Jackson ImmunoResearch).
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bioRxiv - Immunology 2021Quote: ... Cy-5-conjugated donkey anti-rabbit IgG and Alexa fluor-488 F(ab’)2 fragment goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratories (Cambridgeshire ...
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bioRxiv - Immunology 2020Quote: ... Allophycocyanin-conjugated F(ab′)2 goat anti-mouse or donkey anti-human IgG (H+L) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The secondary antibody peroxidase-conjugated AffiniPure F (ab’)2 Fragment Goat Anti-Mouse IgG (H+L) was purchased from Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Immunology 2021Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2021Quote: ... Isolated cells were then cultured in vitro in the presence or absence of 5 mg/ml anti-IgM F(ab)2 (Jackson Immunoresearch) and/or 5 mg/ml anti-CD40 (BioXCell ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% 1X TBS-T followed by 2 hours incubation at room temperature with secondary anti-mouse or anti-rabbit antibody (1:5000, Jackson ImmunoResearch Laboratories ...