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Citations for Jackson ImmunoResearch :
4001 - 4050 of 4192 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse tissue sections to be incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
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bioRxiv - Cell Biology 2022Quote: ... At the next day the coverslips were washed with PBS and incubated with the secondary antibodies (From donkey, 1:500, conjugated with Cy3, Alexa 488, Alexa568, or Alexa647; Jackson Immunoresearch, West Grove, PA) diluted in 10% normal donkey serum in 0.5% Tx-100 (PBS) ...
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bioRxiv - Biochemistry 2024Quote: ... At the next day the coverslips were washed 3 times with PBS and incubated with the secondary antibodies (from donkey, 1:500, conjugated with Cy3, Alexa488, Alexa568, or Alexa647; Jackson Immunoresearch, West Grove, PA) diluted in 10% normal donkey serum in 0.5% Tx-100 (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... wells were incubated with 50 μl of 1:2000 diluted horseradish peroxidase-conjugated goat anti-mouse IgG Fc antibody (Jackson ImmunoResearch,115-035-008) in 4% blocking buffer for 1.5 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The following secondary antibodies were used: Rhodamine Red™-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:200; Cat.#711-295-152; Jackson ImmunoResearch Laboratories, Inc.); Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed four times with PBST and incubated with donkey anti-goat Cy3 tertiary antibody (1:500, Jackson ImmunoResearch, catalog # 705-165-147) in PBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue was dissected to isolate the cochlea and vestibular organs and these were permeabilized for 30 minutes in blocking solution containing PBS with 1% bovine serum albumin (BSA) (Jackson ImmunoResearch 000-001-162) and 5% donkey serum (Jackson ImmunoResearch 017-000-121) ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for 1 hour at room temperature with goat anti-mouse IgG horseradish peroxidase (HRP) (Jackson ImmunoResearch, 115-035-166): IgG1 and IgG2a/b levels were quantified using goat anti-mouse IgG1 HRP (Southern Biotechnology Associates ...
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bioRxiv - Neuroscience 2023Quote: ... sections were light-protected and incubated at room temperature for 2 h with donkey anti- rabbit IgG AlexaFluor®488-conjugated (1:400; Jackson ImmunoResearch, Pennsylvania, USA) and Streptavidin AlexaFluor®594-conjugated (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... They were rinsed in PBS three times (10 minutes each) and incubated in Cy3-conjugated donkey anti-rabbit IgG (1:400; Jackson ImmunoResearch #711-165-152) with 2% NDS for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for two hours the following day with donkey anti-goat Cy5-conjugated secondary antibodies (dilution 1:500, Jackson ImmunoResearch, 703-175-155) and mounted on slides with Fluoromount Aqueos Mounting Medium (Merck ...
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bioRxiv - Pathology 2023Quote: ... Alexa Fluor 488 AffiniPure F(ab’)[Fragment Donkey Anti-Goat IgG (H+L) (1:200, 705-546-147, Jackson ImmunoResearch, West Groove, PA, USA), Donkey anti-Goat IgG (H+L ...
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bioRxiv - Pathology 2023Quote: Slides were then washed and incubated for 60 minutes at room temperature with the secondary antibody: 1:100 dilution of DyLight 594 conjugated mouse anti-FITC (Jackson Immunoresearch 200-512-037).
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bioRxiv - Biochemistry 2022Quote: ... 1:5,000 dilution in PBST) and goat anti-rabbit secondary antibody peroxidase conjugate (1:20,000 dilution in PBST; Jackson ImmunoResearch Laboratories Inc., 111-035-003).
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed 3 × 5 min with TBS-T and incubated with IRDye goat-anti-mouse or goat-anti-rabbit secondary antibodies (1:10,000 Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following secondary antibodies at the indicated dilutions: Cy3-conjugated goat anti-mouse (Jackson ImmunoResearch 115-165-003, 1:1000), Cy3-conjugated goat anti-rat (Jackson ImmunoResearch 112-165-003 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated with the secondary antibody (goat-anti-mouse, FITC conjugated, 1:200 in PBSA, Jackson ImmunoResearch Europe Ltd, #115-095-146) for 1 hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 30 min in a Horse Radish Peroxidase conjugated goat anti-guinea pig secondary antibody (1:100; Cat No. 106-035-003, Jackson ImmunoResearch; West Grove, Pennsylvania) diluted in TNB buffer ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary anti-neurofilament antibody (DHSB, 2H3) was used at a 1:20 dilution and anti-mouse HRP-conjugated secondary antibody (Jackson ImmunoResearch, 115-035-003) was used at 1:1000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... Binding of proteins was examined via Western Blot analysis using anti-Frs2 (SCBT, sc-8318) or anti-Grb2 (CST, 36344) used at 1:1000 dilution and anti-rabbit HRP-conjugated secondary (Jackson ImmunoResearch, 111-035-003) used at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were washed three times in PBS and stained with goat-anti-rabbit Alexa Fluor 647 Fab Fragment (1:800; Jackson ImmunoResearch 111-607-003) for an hour ...
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bioRxiv - Bioengineering 2023Quote: ... the beads were washed once with ice cold PBSB and incubated with a 1:300 dilution of goat anti-alpaca IgG H+L (also reactive for llama antibodies) Alexa Fluor 647 (Jackson ImmunoResearch, 128-605-160) on ice for 4 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... They were rinsed in PBS three times (10 minutes each) and incubated in Cy3-conjugated donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch #711-165-152) with 2% NDS and 0.3% TX for 90 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated in α-rabbit or α-mouse IgG conjugated to TRITC (Jackson ImmunoResearch, #711-025-152 and #715-025-150, 1:300) diluted in 5% sheep serum in PBS ...
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bioRxiv - Neuroscience 2023Quote: Immunopurification was carried out using hydrophobic petri dishes coated overnight with Biotin-SP-conjugated AffiniPure goat anti-rabbit IgG (1:1000; Jackson ImmunoResearch, West Grove, PA) in 50 mM Tris buffer at 4°C followed by another overnight incubation at 4°C with either mouse anti-Ngfrp75 for rat OECs (Suppl ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed three times in 1x PBS and incubated in secondary antibodies of 1:500 goat anti-rabbit Alexa Fluor 647 (Jackson ImmunoResearch; 111-605-144) or 1:500 donkey anti-chicken Alexa Fluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed 3X 15 min in 0.1M PBS and then incubated at 4°C for 24 h with donkey anti-chicken-488 (1:1000; 703-545-155, Jackson Immunoresearch, West Grove, PA) and donkey anti-rabbit-CY3 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... floating sections were washed six times for five minutes in 1x PBS and blocked at room temperature for 1 h in 10% normal goat serum (NGS; Jackson ImmunoResearch; 005-000-121) in 1x PBS with 0.3% Tween-20 (PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in the same fresh medium containing biotinylated goat anti-rabbit IgG antibody (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with 6 nm Colloidal Gold AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 115-195-146, 1:20, AB_2338728) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the appropriate mixture of the secondary donkey anti-rabbit Cy3 (1:700; 711-165-152, Jackson Immunoresearch, West Grove, PA) and donkey anti-chicken-488 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... the blot was washed 3 times with 1× TBST for 15 min each at RT and then incubated with horseradish peroxidase–conjugated goat anti-mouse IgG antibody (1:20000, Jackson ImmunoResearch 115-035-003) diluted in 5% non-fat milk for 1 h at RT ...
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Localization of Cadherins in the postnatal cochlear epithelium and their relation to space formationbioRxiv - Cell Biology 2024Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...
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bioRxiv - Systems Biology 2024Quote: ... Slides were washed once with PBS and blocked for one hour at room temperature in blocking buffer of PBS with 1% Normal Donkey Serum (Jackson ImmunoResearch #017-000-121), 0.1% Gelatin ...
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bioRxiv - Bioengineering 2024Quote: ... Next day, the cells on the slides were incubated with secondary antibodies (donkey anti-rabbit, 1:500) (Jackson ImmunoResearch, Cat. No. 711-605-152) conjugated with Cy5 for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... AffiniPure goat anti-mouse IgG (H+L) (0.4 µg/mL in PBS supplemented with 1% w/v BSA; Jackson ImmunoResearch, West Grove, PA, USA) was added ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Systems Biology 2024Quote: ... Secondary antibodies used in this study were: HRP (horseradish peroxidase)-conjugated Goat-anti-mouse IgG secondary antibody (1:10000; Jackson ImmunoResearch # 115-035-003) and Alexa Fluor™ Plus 647 conjugated goat anti-rabbit IgG secondary antibody (1:3000 ...
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bioRxiv - Neuroscience 2024Quote: ... washed 3 times in PB 0.1 M, a secondary antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, goat anti-rabbit Cyanine Cy3TM (Jackson Immunoresearch, 111-165-003, RRID: AB_2338000), goat anti-mouse Cy5TM (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2024Quote: ... biotinylated Lec-bs IgG was detected by incubating the membranes in streptavidin-peroxidase (1:1000 in same diluent, Jackson ImmunoResearch Labs, West Grove, PA). The membrane was developed using ECL Western blotting substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were washed with PBS and immunostained in PBG with secondary antibodies conjugated to fluorescein isothiocyanate (FITC) (donkey anti-mouse IgG; Jackson ImmunoResearch Lab, Inc., 1:125) or Cy3 (donkey anti-rabbit ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed and incubated at 37°C for 60 min with the appropriate secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X for 10 min each in T-Buffer followed by one wash in TBS and incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, diluted at 1:250) and Alexa Fluor 488-conjugated donkey anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase-conjugated anti-mouse or anti-rabbit (115-035-174 and 211-032-171, Jackson ImmunoResearch Laboratories; both 1:10,000).
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bioRxiv - Neuroscience 2019Quote: ... and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L) (1: 50 dilution; 111-295-003; Jackson ImmunoResearch Europe Ltd, Cambridge, UK) for 1 h at room temperature ...