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Citations for Jackson ImmunoResearch :
351 - 400 of 486 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times for 10 minutes in TBST and incubated in horseradish peroxidase-conjugated secondary antibodies (1:10,000; Jackson ImmunoResearch) at room temperature in TBST with 5% milk for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were incubated in blocking solution (10% IgG-Free BSA, 5% donkey serum or goat serum in PBS, Jackson ImmunoResearch) for 30 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated in blocking buffer containing PBST (0.01% Triton X-100 in PBS, pH 7.4) and 10% normal donkey serum (Jackson Immunoresearch labs) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: ... A Fab fragment affinity purified IgG anti-mouse was applied if anti-mouse antibodies were used (1:10, Jackson ImmunoResearch). The following primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed in TBST (TBS with 0.05% Tween 20) for 30 min (3 × 10 min) and incubated with secondary antibodies conjugated to horseradish peroxidase (Jackson ImmunoResearch) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed in PBS and blocked for 90 min with PBS supplemented with 10 % normal donkey serum (Jackson ImmunoResearch) and 0.2 % Triton X-100 (Carl Roth) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times for 10 minutes with TBST and horseradish peroxidase-conjugated anti-rabbit secondary antibody (Jackson immunoresearch) was incubated for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... The sections were then incubated at room temperature for two hours in blocking solution (10% normal donkey serum (Jackson ImmunoResearch) in PBS-Triton) ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in 1 × TBS (3 × 10 min) before being incubated in a secondary antibody (1:10,000 HRP-conjugated goat anti-rabbit, Jackson ImmunoResearch), in blocking solution described above ...
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bioRxiv - Immunology 2022Quote: ... incubated in 0.1 M glycine for 10 min at room temperature and subsequently incubated in 5% goat serum (Jackson ImmunoResearch) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 × 10 min with PBST (1X PBS with 0.5% Triton X-100) and incubated in appropriate secondary antibodies (Jackson ImmunoResearch) conjugated to Alexa 488 ...
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bioRxiv - Developmental Biology 2022Quote: ... which consisted of 0.1% Triton X-100 in PBS supplemented with 10% normal donkey serum (v/v) (017-000-121, Jackson ImmunoResearch), for at least 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 0.9 % NaCl) and then blocked with PBST containing 10 % normal donkey serum (017-000-121, Jackson Immunoresearch, West Grove, PA) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed with PBS and permeabilized/blocked at room temperature for 1h with PBS with 0.25% Triton-X and 10% normal donkey serum (Jackson ImmunoResearch). Cells were incubated overnight at 4°C with primary antibody in PBS with 0.1% Triton-X and 2% normal donkey serum ...
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bioRxiv - Neuroscience 2023Quote: Tissue sections from both the spinal cords and DRGs were placed in 10% normal donkey serum (Jackson ImmunoResearch, Pennsylvania, USA) in antibody diluent (0.3 M NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were permeabilized with 0.1% Triton X-100 and blocked with a mixture of 10% goat serum (Jackson ImmunoResearch) in PBS for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... 10 nM of biotinylated goat Anti-Mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch Laboratories, Inc., cat. #115-065-003) was loaded into the chamber and incubated for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... B cells were stimulated with 10 μg/mL polyclonal F(ab’)2 goat anti-mouse IgM (anti-μ) (Jackson ImmunoResearch) or 5 μg/mL anti-IgDa (clone AMS 9.1 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by the addition of 10 ng/ml Goat Anti-Armenian Hamster IgG (127-005-099; Jackson ImmunoResearch Laboratory, Inc.) at 37 [for 1 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then incubated at room temperature for one hour in blocking solution (10% normal donkey serum (Jackson ImmunoResearch) in PBS-Triton) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was again rinsed three times in 0.1M NaPb for 10 min followed by 2-h in biotin-SP-conjugated donkey anti-rabbit (1:300; Jackson ImmunoResearch) secondary antibody and AlexaFluor-488 donkey anti-chicken secondary antibody (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then permeabilised with 0.1% Triton X-100 for 10 minutes and then blocked in 5% goat serum (diluted in 0.01M PBS: block, Jackson ImmunoResearch) for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... The floating sections were washed 3×10 min with 1x PBS and blocked with 5% normal donkey serum (Jackson ImmunoResearch), 2% fish gelatin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3×10 min in PBS and then blocked in 0.3% PBST containing 10% normal donkey serum (catalog #17-000-121, Jackson Immunoresearch) for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were incubated for 1 h with donkey anti-rabbit IgG coupled to 10-nm gold particles (Jackson ImmunoResearch) diluted 1:50 with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue was permeabilized and blocked for one hour with PBT (PBS, TritonX-100 (0.3%)) with 10% donkey serum (Jackson ImmunoResearch). Slides were incubated at 4°C with primary (Day 1 ...
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bioRxiv - Neuroscience 2024Quote: ... The next day, 0.3 mL of block solution (0.20% triton, 10% natural goat serum (NGS) (Jackson ImmunoResearch 005-000-121), in 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... To analyze p-ERK levels, unconjugated anti-CD3 (10 μg/ml, 145-2C11) and goat anti-hamster IgG (25 μg/ml, Jackson Immunoresearch) were used to stimulate TCR ...
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bioRxiv - Bioengineering 2021Quote: ... and placed on glass slides as previously described.53 Tissue sections were blocked and permeabilized with TBS (0.1M Tris, 0.15 M Nacl, pH 7.5) containing 10% donkey serum (Jackson ImmunoResearch, 017-000-121), 0.05% Tween-20 (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed with 0.01M PBS (10 Min × 3 times) and incubated in FITC or texas red-conjugated secondary antibody (1:200, Jackson ImmunoResearch Laboratories) in dilution buffer (1% BSA and 0.3% Triton X-100 in 0.01M PBS) ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were incubated with F(ab’)2 goat-anti-mouse IgM+G (10 μg/ml, Jackson ImmunoResearch Laboratories) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Every fourth section was blocked for 2h at room temperature (RT) while shaking in Triton-PBS containing 10% v/v of normal donkey serum (NDS; Jackson Immunoresearch). Next ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating sections were washed in phosphate buffered saline (PBS) and unspecific antibody binding sites were blocked by incubating sections in PBS supplemented with 10 % donkey serum (Jackson ImmunoResearch) and 0.2 % Triton X-100 (Carl Roth ...
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bioRxiv - Cancer Biology 2022Quote: ... Cooled slides were washed 3X in PBS and blocked for 1 hour at room temperature in 10% normal goat serum (Jackson ImmunoResearch) diluted in 5% BSA in PBS with 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed three times for 10 min with PBST and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (HRP anti-rabbit [Jackson ImmunoResearch, 111-035-144] or HRP anti-mouse [Vector Laboratories ...
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bioRxiv - Immunology 2020Quote: ... samples were incubated in a solution containing 10 μg/mL of anti-Rat FITC conjugated antibody (Jackson ImmunoResearch, 712-096-153) and 0.25% of gelatin for 1 hour at RT ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-Kisspeptin 10 antisera (1:8000; gift of Alain Caraty) followed by biotinylated donkey anti-sheep immunoglobulins (1:1500; Jackson Immunoresearch), donkey anti-rabbit conjugated with Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The protocol was slightly modified as above: non-specific binding sites were blocked by 10% normal goat serum (NGS diluted in TBS, Jackson ImmunoResearch) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed three times in PBS (10 min each) and incubated in donkey anti-rabbit Alexa Fluor 594 (1:500, Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... The sections were incubated with 0.3% H2O2 in TBS for 15 minutes to block endogenous peroxidase activity and were incubated with 10% normal horse serum (Jackson ImmunoResearch) in TBS for 30 minutes to block non-specific staining ...
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bioRxiv - Immunology 2021Quote: ... 10 µg/ml biotin-SP AffiniPure F(ab’)2 fragment goat anti-mouse IgM (biotinylated anti-IgM, Jackson ImmunoResearch Laboratories, Inc.), 1 µg/ml recombinant murine CD40L (CD40L ...
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bioRxiv - Biophysics 2021Quote: ... Non-specific staining was limited through a blocking step using 10% (w/v) Bovine Serum Albumin (BSA) (Jackson ImmunoResearch, PA, USA) for 2 hours at room temperature (measured at 22-23°) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS three times and then incubated in blocking solution (PBS supplemented with 0.1% Triton-X-100 [X100, Sigma-Aldrich] and 10% normal donkey serum [017-000-121, Jackson ImmunoResearch]) for at least one hour at RT ...
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bioRxiv - Cell Biology 2023Quote: ... + 10% FBS first and then with IF Buffer + 10% FBS + 20 µg/ml goat anti-Rabblit Fc fragment (111-005-046, Jackson ImmunoResearch). Acini were incubated with the primary antibodies in the secondary block solution washed with IF buffer and then incubated with secondary antibody in IF Buffer +10% FBS ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then washed 3 times for 10 min with PBS and stained with the suitable secondary antibody (1:200, Jackson ImmunoResearch) and DAPI (4,6-diamidino-2-phenylindole dihydrochloride ...
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bioRxiv - Immunology 2023Quote: ... 12-well slides were coated with 10 μg/ml F(ab’) Fragment Goat Anti-Mouse IgM (Jackson ImmunoResearch, 115-006-020) in PBS for 1 hour at room temperature or fibronectin as a control ...
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bioRxiv - Cell Biology 2022Quote: ... sections were blocked for 1 hour at room temperature in blocking buffer: 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121) in PBST (0.1% TritonX-100 in PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121, Jackson ImmunoResearch) diluted in 1x PBS (blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 1 h with a biotinylated donkey antibody to guinea pig IgG (10 μg/ml; 706-065-148, Jackson ImmunoResearch). The sections were further incubated with Alexa594-conjugated streptavidin (2.5 μg/ml).
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bioRxiv - Cancer Biology 2023Quote: ... B cells were plated at a concentration of 5L×L105 cells per 200LμL in round-bottom 96-well plates treated with 0.1% B16F10 melanoma cell lysis or 10 µg/mL goat anti-mouse IgM F(ab’)2 (115-006-020, Jackson ImmunoResearch). B cells were treated with NR-V04 or DMSO for 24 hours and collected for flow cytometry analysis.