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Citations for Jackson ImmunoResearch :
351 - 400 of 457 citations for Recombinant Rat Ghr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... or Cy2-conjugated anti-mouse and Cy5-conjugated anti-rat IgGs (1:200; Cat#: 115-225-146, RRID:AB_2307343; Cat#: 112-175-143, RRID:AB_2338263; Jackson ImmunoResearch, Cambridge, UK) were used ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary antibodies used: Alexa Fluor 594 conjugated donkey anti-rat IgG (Jackson ImmunoResearch – 1:1000 dilution, 712-586-153), Alexa Fluor 488 conjugated donkey anti-chicken IgY (Jackson Immunoresearch ...
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bioRxiv - Genetics 2022Quote: ... then secondary antibodies anti-rat Alexa-CY3 (1/150) or with anti-goat Alexa-488 (1/150) (Jackson ImmunoResearch), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... a secondary antibody reaction was performed using anti-rat IgG biotinylated antibodies (1:200, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Immunology 2023Quote: ... and Alexa Fluor 647 conjugated donkey anti-rat secondary antibody (1:300, Jackson ImmunoResearch Lab, Cat# 712-605-153) for 90 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained with Alexa Fluor ® 594-conjugated mouse anti-rat IgG (Jackson ImmunoResearch, 212-585-104) for 30 minutes on ice and in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... Cy5-donkey anti-mouse IgG and Cy5-goat anti-rat IgG (1:2,000; 715-175-151 and 1:2,000; 712-175-153, respectively, Jackson ImmunoResearch).
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bioRxiv - Immunology 2020Quote: ... a secondary Cy5-conjugated AffiniPure donkey anti-rat IgG (H+L) (1:200, stock of 0.5 mg/ml, Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... and goat-anti-mouse (cat#115-035-003, RRID:AB_10015289) or goat-anti-rat (cat#112-035-003, RRID:AB_2338128) horseradish peroxidase (HRP) secondary (Jackson ImmunoResearch Labs; 1:10,000).
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS (3 times) and stained with Cy3-conjugated donkey anti-rat IgG (Jackson ImmunoResearch Laboratories; 1.25 µg/ml). To detect total channels ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with anti-rabbit-Cy2 and anti-rat-Cy3 secondary antibodies for 2 h (1:400, Jackson Immunoresearch, #711-225-152 ...
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bioRxiv - Immunology 2022Quote: ... followed by a washing step in permbuffer and incubation with a biotinylated secondary antibody against the primary anti-RORγt antibody (Biotinylated anti-rat; Jackson ImmunoResearch). To detect RORγt+ ILC and B cells ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies (dilutions 1:1000): anti-rat IgG to detect 3F10 (Jackson ImmunoResearch 712-165-153 or 712-545-153) and appropriate second antibody (mouse ...
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bioRxiv - Genetics 2020Quote: ... embryos were incubated overnight with donkey anti-rat HRP secondary antibody at 1:1000 dilution (Jackson ImmunoResearch, 712-036-153) followed by incubation with 488-tyramide reagent at 1:25 dilution made using Dylight 488 NHS ester (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... a secondary antibody of either donkey anti-rat or donkey anti-guinea pig Alexa Fluor 488 (1:500, Jackson ImmunoResearch) was applied overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... (Tomancak et al., 1998)) and subsequently with secondary antibodies Alexa 647 conjugated donkey anti-rat IgG (1:1000; Jackson ImmunoResearch). Ovaries from flies expressing fusion proteins were fixed in 2% PFA and 0.01% Triton X-100 for 15 min at RT ...
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bioRxiv - Microbiology 2019Quote: ... Each staining was performed in the presence of purified anti-mouse CD16/32 (clone 93) and 0.2 mg/ml purified rat gamma globulin (Jackson Immunoresearch). All antibodies were purchased from eBioscience (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies included donkey anti-rat IgG-Cy3 and Cy5 (Jackson ImmunoResearch, 712-165-153, 706-175-148 1:1000). DAPI nuclear stain was co-applied with secondary antibody at a final concentration of 0.5 µg/ml.
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bioRxiv - Cell Biology 2023Quote: ... AffiniPure F(ab’)2-goat anti-rat IgG (H+L) Alexa 488 (Jackson ImmunoResearch Labs Cat# 112-546-003, RRID:AB_2338364), F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109 ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibodies for western blotting were horseradish peroxidase–conjugated anti-rabbit and horseradish peroxidase–conjugated anti-rat (Jackson ImmunoResearch).
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bioRxiv - Cell Biology 2023Quote: ... or allophycocyanin-conjugated (APC) AffiniPure F (ab’)2 fragment goat anti-Rat IgM (1:100, Jackson ImmunoResearch, 112-136-075) at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST; Jackson ImmunoResearch Laboratories Inc., 415-035-166). SPRTN was detected using SPRTN anti-serum (Rb 31053 ...
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bioRxiv - Microbiology 2023Quote: ... for 1 hr and detected with donkey anti-rat Alexa Fluor 594 (Jackson ImmunoResearch, Cat# 712-585-153, 1:500) and goat anti-rabbit Alexa Fluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 647-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) (Jackson ImmunoResearch Labs Cat# 111-605-144 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in a secondary antibody solution containing 1:500 donkey anti-rat Cy3 (Jackson ImmunoResearch; PA, USA) in 0.1 M PBS for 24 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies (FITC-conjugated goat anti–rabbit, rhodamine-conjugated goat anti–mouse, and Cy3-conjugated goat anti–rat; Jackson ImmunoResearch Laboratories) were used at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed for 15 min with 1x PBS/0.1% Tween20 post primary antibody treatment followed by 1 hour incubation at room temperature with 1x PBS+3%BSA containing secondary antibodies anti rabbit IgG-Alexa 488 or anti rat IgG-Alexa 594 (all from Jackson ImmunoResearch). The nuclear stain DAPI (2μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulins (IgGs) (115-036-062) and HRP-conjugated goat anti-rat IgGs (112-035-167) were purchased from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with secondary antibodies R-Phycoerythrin AffiniPure F (ab’) 2 Fragment Donkey Anti-Rat IgG (1:300; 712-116-153; Jackson ImmunoResearch) or Alexa Fluor 568 donkey anti-rabbit IgG (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies (donkey anti rabbit AlexaFluor® 488, and goat anti rat Alexa 594; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), were incubated at 1:400 dilution in 1% BSA with 5% goat and 5% donkey serum in PBS solution ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated for 2 hr at room temperature with corresponding Alexa-conjugated secondary antibodies diluted to 1:800 in blocking solution (goat anti-rabbit Alexa 488 and goat anti-rat Alexa 594 antibodies, Jackson ImmunoResearch). Sections were washed ...
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bioRxiv - Neuroscience 2021Quote: ... and a selection step (primary: anti rat Thy1.1/CD90 clone T11D7e; secondary: goat anti-mouse IgM, Jackson ImmunoResearch 115-005-003) and plated on PDL-coated 96-well microplates (7000 cells per well ...
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bioRxiv - Cancer Biology 2021Quote: ... The secondary antibodies Biotin-SP-AffiniPure donkey anti-rabbit IgG (H+L) (#711-065-152) and Biotin-SP-AffiniPure donkey anti-rat IgG (H+L) (#712-065-153) were from Jackson ImmunoResearch Laboratories.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated in secondary antibody (1:300, Alexa Fluor 488, or AffiniPure Mouse Anti-Rat IgG AMCA conjugate, Jackson Immunoresearch) for 2 hours at room temperature ...
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bioRxiv - Physiology 2022Quote: ... The secondary antibody Duolink In Situ PLA Probe Anti-Mouse MINUS from the PLA kit was replaced with a Donkey anti-rat antibody (catalog no 712-005-150, Jackson ImmunoResearch) coupled to a MINUS probe using the Duolink In Situ Probemaker MINUS kit (catalog no ...
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bioRxiv - Neuroscience 2022Quote: ... F4/80 and Ly6B.2 antibodies were visualized with an anti-rat-488 antibody and NR4A1 was visualized with an anti-goat-594 antibody (1:100; 45 min, Jackson ImmunoResearch). Human brain sections were rehydrated and antigen demasking was done using EnVisionTM FLEX Target Retrieval Solution (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants from the hybridomas were then added and allowed to incubate for one hour at room temperature followed by the addition of goat anti-rat IgG (heavy chain specific)-HRP (Jackson ImmunoResearch) at a 1:5,000 dilution in blocking solution for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary antibodies were used at 1:500 and included donkey-anti-rabbit AlexaFluor-488 and/or donkey-anti-rat AlexaFluor-647 (Jackson ImmunoResearch) and/or donkey anti-Sheep AlexaFluor-555 (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... in 500 μL of RIPA buffer were incubated overnight at 4°C with unspecific mouse/rabbit/rat IgG antibodies (Jackson Immunoresearch) or rat anti-HA-tag (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed 3 × 5 minutes in PBS prior to a 2 hour incubation with a biotin-conjugated donkey anti-rat secondary antibody (1:500, 712-065-153, Jackson ImmunoResearch). The primary–secondary antibody complexes were detected via peroxidase-driven precipitation of diaminobenzidine (DAB) ...
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bioRxiv - Immunology 2020Quote: ... samples were incubated in a solution containing 10 μg/mL of anti-Rat FITC conjugated antibody (Jackson ImmunoResearch, 712-096-153) and 0.25% of gelatin for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... 2016)) and secondary antibodies were anti-rat coupled to Alexa Fluor 488 and anti-rabbit coupled to Cyanine 3 (Jackson ImmunoResearch) both diluted to 1:500 ...
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bioRxiv - Immunology 2022Quote: ... Cells were treated with 50 μl of Fc block (0.1% rat gamma globulin [Jackson ImmunoResearch], 1 μg/ml of 2.4G2 [BioXCell]) for 10 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... to different IgG antibodies respectively (anti-mouse 715-005-151, anti-rabbit 711-005-152, anti-rat 712-005-153; Jackson ImmunoResearch).
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bioRxiv - Cancer Biology 2022Quote: ... diluted 1:300 in blocking buffer and with the Cy3 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) antibody (#712-165-513, Jackson ImmunoResearch) diluted 1:150 in blocking buffer ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated with secondary antibodies diluted 1:200 v/v in blocking buffer solution for 2h at room temperature (anti-rat ALexa488, anti-mouse Alexa546, Jackson Immunoresearch). After incubation with secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... After three times 10min washing with 1% BSA in PBS consecutive incubation using Alexa Fluor 488-AffiniPure F(ab’)2 fragment donkey anti-rat IgG (H+L) secondary antibody and streptavidin-Cy3 secondary antibody (both Jackson ImmunoResearch) was done ...
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bioRxiv - Cell Biology 2020Quote: ... Donkey anti-chicken Alexa-Fluor 488 (703-545-155, 1/500) and donkey anti-rat Alexa-Fluor 405 (712-475-153, 1/100) were obtained from Jackson ImmunoResearch.
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bioRxiv - Immunology 2021Quote: ... cells (1 × 106) were washed with staining buffer and incubated with whole rat and mouse IgG (Jackson ImmunoResearch, West Grove, PA) for 30 min on ice to block Fc receptors ...