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Citations for Jackson ImmunoResearch :
3501 - 3550 of 3809 citations for SpectraDye Antibody Labeling Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS-T for 5 minutes (3x) and incubated for 2 hours at room with secondary antibody (AlexaFluor-488 1:100, Jackson ImmunoResearch Laboratories, 703545145). Slices were mounted onto slides (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in the secondary AlexaFluor-488-coupled goat anti–chicken antibody (1:700; Jackson Immunoresearch, catalog no. 103-545-155) with 2% NGS and 0.4% Triton X-100 in 0.1 M PBS at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... specimens were rinsed 5 times in PBS and treated for 2 h at room temperature in species-specific secondary antibodies conjugated to either DyLight 405 (1:500, Jackson ImmunoResearch Laboratories Inc.), or AlexaFluor-488 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h at room temperature in a solution containing a biotinylated goat anti-rabbit secondary antibody (Jackson Immunoresearch, 1:2000). Sections were washed again and processed with the avidin-biotin complex for 2 hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... washed and overlaid with HRP-conjugated goat anti-mouse secondary antibodies diluted 1:5,000 in blocking solution for 1h at RT (Jackson ImmunoResearch, #115-035-003) and subsequently washed ...
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bioRxiv - Pathology 2023Quote: ... sections were washed with 1X TBS and stained with Alexa Fluor 594 conjugated donkey anti mouse secondary antibody (1:1000, Jackson Immunoresearch, RRID: AB_2340857) and Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch, West Grove, PA). Sections were then mounted on slides (ProLong Gold ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were subsequently washed with PBS-T and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-mouse-IgG (115-035-146, Jackson ImmunoResearch, 1:2,000) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with an anti-rabbit IgG horseradish peroxidase-conjugated (HPR) secondary antibody (1:10,000) (cat #211032171, Jackson ImmunoResearch, West Grove, PA) and an anti-mouse IRDye 800CW (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... for 10 minutes and probed for 1 hour at room temperature with the horseradish peroxidase-conjugated mouse anti-rabbit IgG monoclonal antibody at a 1:5,000 dilution (Jackson ImmunoResearch, 211-035-109). Membranes were washed three more times in TBST before protein expression was detected using the Pierce ECL Plus Substrate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... molecular weight bands were visualized by peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson ImmunoResearch Laboratories, West Grove, PA). ECL-plus (Western Lighting ...
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bioRxiv - Neuroscience 2023Quote: ... Ximbio #155259) and the secondary antibody set included either AlexaFluor 594-conjugated donkey anti-chicken IgY (1:500; Jackson ImmunoResearch #703-585-155) or AlexaFluor 546-conjugated goat anti-chicken IgY (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... all membranes were incubated with HRP-conjugated goat anti-mouse or anti-rabbit secondary antibody (Jackson ImmunoResearch, West Grove, PA; 1:2000 dilution) corresponding to the primary antibody and developed with the enhanced chemiluminescence method ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 hours with a secondary anti-rabbit antibody conjugated to Alexa 647 (1:500; Jackson Immunoresearch, 111-605-045) with 5% normal goat serum in PBS + 0.3% Tween-20.
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in donkey anti-mouse Cy3 secondary antibody (1:200 in PBS, 715-165-020, RRID: AB_2340811; Jackson ImmunoResearch, West Grove, PA) for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibodies were incubated for 1 hour at room temperature (goat anti-rabbit 488 Jackson ImmunoResearch Labs, 1:500 in PBS+0.5% Tween). Samples were rinsed with PBS+0.5% Tween 3 × 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in 0.1M PBS and incubated in donkey anti-rabbit horseradish peroxidase-conjugated secondary antibody (1:500, catalog #711-036-152, Jackson ImmunoResearch; RRID: AB_2340590) for 1hr ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove, PA, USA). The ImmPRESS-Alkaline phosphatase polymer kit was purchased from Vector Laboratories (Burlingame ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were washed 3x with 1 mL of FACS buffer before 30 minutes of staining in FACS buffer with a 1:1000 dilution of anti-mouse IgM Alexaflour-488 secondary antibody (Jackson Immunoresearch, Cat#715545140) on ice ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies: donkey anti-rabbit IgG-HRP (Jackson ImmunoResearch, 711-035-152; 1:5,000); donkey anti-mouse IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were made in donkey or goat and conjugated with: A488 (anti-chicken, 1:500, Jackson Immunoresearch, Ely, UK, 703-545-155); A488 (anti-goat ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed three times for 10 min and incubated with a 1:10,000 dilution of horseradish peroxidase-conjugated anti-rabbit antibodies (Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed 3X 5 minutes in TBS + 0.1% Tween and then incubated for 1 hour in milk containing donkey-anti-mouse IgG secondary antibodies conjugated with HRP (Jackson ImmunoResearch Laboratories, Inc.) diluted at 1:5000 in block ...
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bioRxiv - Neuroscience 2023Quote: ... 1:75,000) against human kisspeptin-54 (aa 68-121 of NP_002247.3) (9, 53) using biotinylated secondary antibodies (Jackson ImmunoResearch Europe, Cambridgeshire, UK; 1:500), the ABC Elite reagent (Vector ...
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bioRxiv - Developmental Biology 2024Quote: ... was used in a 1:300 dilution followed by anti-rabbit HRP secondary antibody diluted to 1:2500 (Jackson ImmunoResearch 111-035-003). For the loading control ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4) and incubated with a biotinylated goat anti-rat polyclonal secondary antibody (1:300; Jackson ImmunoResearch, Baltimore, USA, cat# 112-065-167) in TBS containing 0.05% Triton X-100 for 90 min at RT ...
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bioRxiv - Immunology 2023Quote: ... The monolayers were labeled with a rabbit anti-chlamydial antibody (raised by immunization with C. muridarum EBs) and a goat anti-rabbit IgG conjugated with Cy2 (green, Jackson ImmunoResearch Laboratories, Inc) to visualize chlamydial inclusions while a Hoechst dye (blue ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 hours with the secondary donkey anti-sheep biotinylated antibody (Cat: 713-065-147, Jackson ImmunoResearch Inc, PA, USA) diluted at a concentration of 1:500 in TBST + 1% NDS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 x 15 min with 0.1 M PBS prior to a 2-hour incubation in secondary antibodies FITC (1:50 in 0.1 M PBS, Jackson ImmunoResearch, 703-095-155), Cy3 (1:50 in antibody diluent ...
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bioRxiv - Neuroscience 2023Quote: ... followed by tyramine signal amplification and treatment with an Alexa Fluor 647-conjugated streptavidin antibody (1:200; Jackson ImmunoResearch Laboratories; RRID: AB_ 2341101). After rinsing with three PBS exchanges (10 min each) ...
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bioRxiv - Genomics 2023Quote: ... 3 times at room temperature and then incubated with secondary antibodies: Cy3 donkey anti-goat (Jackson ImmunoResearch, Cat# 715-165-150, 1:500), Alexa Fluor 488 donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Pathology 2023Quote: ... and further incubated for 1h at room temperature with cy3 AffiniPure F(ab’)2 fragment rabbit anti-goat IgG and alexa fluor 647 AffiniPure donkey anti-rat IgG secondary antibodies (Jackson ImmunoResearch Europe Ltd). Slides were mounted with Vectashield mounting medium with DAPI (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... and remaining cell surface antibody blocked by incubation with 0.13 mg/ml F(ab’)2 fragment goat-anti rabbit IgG (Jackson Immunoresearch, 111-006-045) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... The wells were probed with horseradish peroxidase (HRP)-conjugated secondary antibody [goat anti-mouse-IgG (Jackson ImmunoResearch, catalog #115-005-003; 1:4000)] for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was washed 3 times for 5 min each with 1X PBS + 0.2% Triton-X and then incubated overnight in blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 1:500). Upon completion of incubation with the secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Identification of different cells and structures in spinal cord and brain was performed using primary antibodies diluted in PBS blocking solution containing 10% normal donkey serum (Jackson ImmunoResearch, Cambridge, UK) and 0.3% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed and incubated for 2h at RT with donkey anti-mouse Cy3 or donkey anti-rabbit Alexa 488 secondary antibodies (Jackson Immunoresearch, Suffolk, UK). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were Alexa Fluor® 488 conjugated anti-rabbit and Alexa Fluor® 488 conjugated anti-mouse (Jackson ImmunoResearch, 1:200 diluted). After staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 1:500 mouse anti-Myc (CST #2276) and 1:3000 goat anti-mouse HRP secondary antibody (Jackson ImmunoResearch #115-035-003). For the loading control ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
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bioRxiv - Physiology 2022Quote: ... followed by incubation with secondary Alexa-488-conjugated donkey anti-rabbit or Cy3-conjugated goat anti-mouse antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (CY5 Donkey Anti-Mouse; Jackson ImmunoResearch Labs; Cat# 715-175-151. CY3 Donkey Anti-Chicken; Jackson ImmunoResearch Labs; Cat#703-165-155) were spun in a centrifuge for 10 minutes at 13000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... the coverslips were incubated with Fab fragment of goat anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor-594 (Jackson Immunoresearch, West Grove, PA) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488-conjugated goat-anti-rabbit (1:500) and Alexa Fluor 647-conjuated goat-anti-mouse (1:800) secondary antibodies (Jackson Immunoresearch; West Grove, PA) were diluted in a solution consisting of 2% goat serum ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue sections incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
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bioRxiv - Neuroscience 2019Quote: ... Then membranes were rinsed in Tris-Buffer (pH =7.4) with 0.1 % of Tween-20 and incubated with HRP-conjugated secondary antibodies – anti-mouse IgG (Jackson Immunoresearch, 715-005-150, Ely, UK) or with anti-rabbit IGG (Abcam ...