Labshake search
Citations for Jackson ImmunoResearch :
301 - 350 of 368 citations for 4 Hydroxy 7 trifluoromethyl quinoline 3 carbohydrazide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... the sections were first washed in PBS 5mins for 3 times and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA), 1% bovine serum albumen (A2153 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Extended Data Table 1) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were washed with PBS and blocked with 2% BSA (w/v) (Roth) and 4% donkey serum (v/v) (Jackson Immunoresearch Laboratories) in PBS for 30 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times in PBS and incubated for 60–90 min at room temperature or overnight at 4°C with the Alexa Fluor 488- and 594 and 647-conjugated secondary antibodies (1:500; Thermo Fisher Science or Jackson ImmunoResearch).
-
bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
-
bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
-
bioRxiv - Neuroscience 2024Quote: ... primary antibody overnight at 4°C and secondary antibody (anti-rabbit-Alexa Fluor 647, 1:400, Jackson ImmunoResearch, Inc., West Grove, PA) for 3-4 hours in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500, Jackson ImmunoResearch #706606148). Tissues were rinsed and mounted using Prolong diamond antifade (Life Technologies) ...
-
bioRxiv - Physiology 2024Quote: ... treated with 1M HCl for 1 hour and incubated overnight at 4°C with anti-BrdU antibody following incubation with Alexa Fluor-488 conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). Slides were mounted in mounting medium for fluorescence with DAPI (Vector Laboratories) ...
-
bioRxiv - Biophysics 2024Quote: ... Nonspecific cross-reactivity was blocked by incubating the samples for 4 h in 1% BSA/PBS (Jackson ImmunoResearch, West Grove, PA, USA). The cells were then incubated with primary antibodies anti-RyR (IgG1 ...
-
bioRxiv - Biochemistry 2021Quote: ... then probed with H2.3 primary rabbit polyclonal anti-HSulf-2 antibody (dil. 1/1000) followed by fluorescent-conjugated secondary anti-rabbit antibody (Jackson ImmunoResearch Laboratories, dil. 1/500). All the incubations were performed for 2 h at 4 °C in TBS ...
-
bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were washed and then incubated 3 h with a secondary antibodies cocktail of Cy3 donkey anti-rabbit (1:600; Jackson ImmunoResearch; #711-165-152, AB_2307443) and Cy2 donkey anti-chicken (1:400 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
-
bioRxiv - Immunology 2024Quote: ... excess primary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by incubation of donkey anti-mouse Cy3 secondary antibody (Jackson Immunoresearch, Cat# 715-167-003) for 1 h at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... Clone SMI-31 reacts with a phosphorylated epitope of neurofilament in mammals and neurofilament 220 in squid51–53 .The secondary Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (Cat#: 115-545-003) and Cy™3 AffiniPure Donkey Anti-Mouse IgG (Cat#: 715-165-151) were purchased from Jackson ImmunoResearch (West Grove, PA) and employed at 1:500 dilutions.
-
bioRxiv - Neuroscience 2021Quote: ... animals were incubated overnight at 4°C in Alexa Fluor 488 donkey anti-chicken secondary antibody (1:1000, Jackson ImmunoResearch, 703-545-155), Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000 ...
-
bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch, Westgrove, PA; 1:10,000) for 1 hr ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.2% Triton X-100 and incubated overnight at 4°C with a CY5-conjugated secondary antibody (1:400, goat anti-mouse, Jackson Immunoresearch, Suffolk, UK), washed in 1x PBS and embedded in 1x PBS ...
-
bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
-
bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
-
bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (4°C overinight) and then secondary peroxidase-conjugated Goat Anti-Rabbit IgG (Jackson ImmunoResearch 111-035-144) or peroxidase-conjugated Goat Anti-Mouse IgG (Jackson Immuno Research 115-035-174 ...
-
bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
-
bioRxiv - Cell Biology 2022Quote: ... 4 µg/ml) and donkey (711-166-152, 711-546-152, 715-166-150 or 715-546-151, Jackson ImmunoResearch, 1.5 µg/ml) secondary antibodies ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were then incubated with a corresponding secondary antibody conjugated at room temperature for 1 h (anti-rabbit Alexa Fluor 488, anti-mouse Alexa Fluor 647, anti-goat Cy™3, Jackson ImmunoResearch Inc., West Grove, PA) in 1% BSA in PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with the same buffer (3 × 10 min) and incubated for 1 hour either with Horseradish-Peroxydase (HRP) goat anti-rabbit IgG (1:3000, Jackson Immunoresearch, Cambridge, UK, #111-035-144) or with HRP Goat anti-mouse IgG (1:5000 ...
-
bioRxiv - Biochemistry 2020Quote: ... The α-actin secondary antibody was a Cy-3 conjugated AffiniPure donkey anti-mouse at a dilution of 1:500 (Jackson ImmunoResearch Laboratories Inc. #715-166-150). Bands for p-ERK ...
-
bioRxiv - Neuroscience 2020Quote: ... selected coronal sections were rinsed three times in TBS and incubated in blocking solution (3% normal donkey serum, Jackson Immunoresearch AB2337258; 0.3% Triton X-100 in TBS) at room temperature for 30 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with primary antibodies (see Key Resources Table) overnight and subsequent incubated with secondary antibodies labeled with Alexa Fluor 488- (Jackson ImmunoResearch, 115-545-003, 3 μg/mL) or Cy3- (Jackson ImmunoResearch ...
-
bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
-
bioRxiv - Systems Biology 2021Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
-
bioRxiv - Neuroscience 2020Quote: ... and the following secondary antibodies at 4°C for 24 h: Alexa Fluor 488 donkey anti-chicken (1:250, Jackson ImmunoResearch, #703-545-155) and Alexa Fluor 568 donkey anti-rabbit (1:250 ...
-
bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 4-6 h in the following secondary antibodies: donkey anti-rat 488 (1: 500; Jackson Immunoresearch, 712-546-153), donkey anti-rabbit-Cy3 (1:500 ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were treated with 1 μM biotinylated peptides for 1h before they were fixed with 4% PFA and incubated with Streptavidin-AlexaFluor 594 antibody (Jackson ImmunoResearch, West Grove, PA) and Hoechst 33342 (Novus Biologicals ...
-
bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature [6-8,10] ...
-
bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
-
bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
-
bioRxiv - Bioengineering 2022Quote: ... Staining with primary antibodies was performed for 24 h (at 4 °C) with subsequently 2 h incubation with secondary antibodies (Jackson ImmunoResearch, West Grove, PA) diluted with 1 % BSA ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
-
bioRxiv - Neuroscience 2023Quote: ... slides were washed 3X 15 min in 0.1M PBS and then incubated at 4°C for 24 h with donkey anti-chicken-488 (1:1000; 703-545-155, Jackson Immunoresearch, West Grove, PA) and donkey anti-rabbit-CY3 (1:400 ...