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Citations for Jackson ImmunoResearch :
3401 - 3450 of 4223 citations for DCN1 like protein 1 DCUN1D1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... The blots probed with α-Nrl antibody were detected using 1:5000 goat α-rabbit HRP secondary antibody (Jackson Immunoresearch catalogue #111-005-003 ...
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bioRxiv - Physiology 2020Quote: ... overnight at 4 °C and then with a donkey anti-guinea-pig Cy5-conjugated antibody for 2.5 hours at RT (1:200, Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked again and incubated with secondary antibodies at 1/250 dilution (Alexa Fluor 647, 555 and 488 from Jackson ImmunoResearch) and/or 1/100 dilution of Alexa Fluor 647 Phalloidin (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... (Tomancak et al., 1998)) and subsequently with secondary antibodies Alexa 647 conjugated donkey anti-rat IgG (1:1000; Jackson ImmunoResearch). Ovaries from flies expressing fusion proteins were fixed in 2% PFA and 0.01% Triton X-100 for 15 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Mouse or rabbit secondary antibodies conjugated to Alexa488 or Cy5 (as appropriate) were used at 1/200 each (Jackson ImmunoResearch). Samples were mounted in VectaShield™ and imaging was performed using a Leica SP5 or SP8 confocal microscope ...
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bioRxiv - Immunology 2019Quote: ... coverslips were stained for 1 hour at room temperature with phalloidin-eFluor660 (eBioscience) or donkey anti-mouse-AF594 (Jackson ImmunoResearch), and mounted onto slides with Vectashield Mounting Medium containing DAPI (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.01% Triton X) and incubated with Secondary antibody (1:400 in blocking buffer, Donkey anti-Rb alxaflour 488 Jackson ImmunoResearch) and TMR-Halo-Ligand (2nM ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were used: Cy3-conjugated anti-armenian hamster (1:200; Jackson ImmunoResearch, cat. no. 127-165-160), AlexaFluor 488-conjugated anti-guinea pig (1:200 ...
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bioRxiv - Biochemistry 2020Quote: ... The anti-His blot was then probed with 1:20,000 horseradish peroxidase-conjugated rabbit anti-mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch) in 5% non-fat milk in PBST for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation (2 h) with appropriate fluorescent secondary antibodies (Cy3-or Cy2-coupled fluorescent secondary antibodies, 1:200; Jackson ImmunoResearch Laboratories catalog #715-165-150 and #715-545-150 respectively) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated at 4°C overnight and followed by incubation of 488- and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch, 1:500) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used in this study: Donkey anti-Guinea pig Alexa Fluor 647 1:500 (Jackson Immunoresearch 706-605-148), Donkey anti-Rabbit Alexa Fluor 488 1:1000 (ThermoFisher A-21206) ...
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bioRxiv - Neuroscience 2021Quote: ... and then blocked for 1 hour at room temperature in 5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA)/0.5% TTBS ...
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bioRxiv - Neuroscience 2021Quote: ... brains were left rocking at 4°C for 1-3 nights in 0.1% PBS-T supplemented with blocking agent normal goat serum (Jackson ImmunoResearch) at a 1:20 dilution ...
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bioRxiv - Genetics 2021Quote: ... the slides were incubated with the AlexaFluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:300; Jackson ImmunoResearch) for 30 minutes After a further wash of 15 minutes in PBT ...
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bioRxiv - Genetics 2021Quote: ... all slides were incubated with the fluorescein (FITC)-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (1:150; Jackson ImmunoResearch) for 1 hour at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated with peroxidase-linked anti-mouse or anti-rabbit IgG (1:10,000) (Jackson ImmunoResearch Laboratories, West Grove, PA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed and incubated with secondary antibody conjugated to Alexa Fluor 488 (Jackson Immunoresearch 711-545-150, 1:500) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... After staining with the anti-mouse horse-radish peroxidase (HRP)-labeled secondary antibody at 1:10,000 (cat. #715-035-151, Jackson ImmunoResearch), the blots were imaged using the SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... room temp) in 0.1% PBST containing the appropriate fluorophore-conjugated secondary antibody (AlexaFluor 488 or 594, 1:2000; Jackson ImmunoResearch). Triton X-100 was included to allow secondary antibody to reach all cellular compartments ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated for 1.5 h at RT with HRP-conjugated antibodies diluted in the blocking solution (1:5,000) against mouse (Jackson ImmunoResearch, AB_2340770) and rabbit (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... Both HRP-conjugated goat anti-rabbit-IgG and goat anti-mouse-IgG antibodies (1:5000) were purchased from Jackson ImmunoResearch Laboratories.
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bioRxiv - Neuroscience 2022Quote: ... All secondary antibodies had a 1:500 dilution and stored overnight at 4 °C (Jackson Immunoresearch; West Grove, PA, USA). Sections were then mounted and coverslipped using 50% glycerol in 0.02 M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Cell Biology 2022Quote: ... MilliporeSigma) was applied for 2 h at RT followed by secondary antibody (fluorophore-conjugated IgG, 1:500; Jackson ImmunoResearch Laboratories) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody was also used followed by a donkey anti-rabbit secondary antibody conjugated to AF488 (1:500, Jackson Immunoresearch).
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibodies used were donkey anti-rabbit and donkey anti-goat at 1:5000 dilutions (Jackson ImmunoResearch, West Grove, PA). Blots were developed using either Clarity Western ECL Substrate (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: Secondary antibodies used for Western blot were Horseradish peroxidase goat antimouse (Jackson ImmunoResearch, cat. no. 115-035-146, 1:10000), Horseradish Peroxidase goat anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... Both HRP-conjugated goat anti-rabbit-IgG and goat anti-mouse-IgG antibodies (1:5000) were purchased from Jackson ImmunoResearch Laboratories.
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bioRxiv - Cell Biology 2022Quote: ... the membrane was incubated with horseradish peroxidase-conjugated goat anti-Mouse IgG (1:1000 dilution, Jackson ImmunoResearch, 115-035-146) overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... the primary antibody was detected utilizing Alexa-Fluor594 anti-mouse IgG (H+L) (1:1000, 715-586-151, Jackson ImmunoResearch) and Alexa-Fluor488 anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated overnight at 4 °C in blocking solution with AffiniPure Fab Fragment (donkey anti mouse, 1:200, catalog #rid_000053, Jackson ImmunoResearch). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed and incubated with HRP conjugated secondary antibodies against rabbit IgG (1/10,000, 711-035-152, Jackson Immunoresearch), goat IgG (1/10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies included donkey anti-rat IgG-Cy3 and Cy5 (Jackson ImmunoResearch, 712-165-153, 706-175-148 1:1000). DAPI nuclear stain was co-applied with secondary antibody at a final concentration of 0.5 µg/ml.
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 1 h with a secondary mouse block (AffiniPure Fab Fragment IgG, 30 μg/ml, Jackson ImmunoResearch) before primary antibody mouse α-NET (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa dye-conjugated secondary antibodies generated in donkey toward the appropriate species (1:500; Alexa488, Alexa594, and Alexa647; Jackson ImmunoResearch) were used as detection antibodies.
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBS-T and incubated for one hour at room temperature in HRP-conjugated goat anti–rabbit or goat anti-mouse secondary antibody (1:10,000, Jackson Immunoresearch) in 5% milk/PBS-T after which membranes were washed three times with PBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated for 1 hour with gold-conjugated secondary antibodies (Jackson ImmunoResearch, #115-205-166 and 111-195-144). Sections were stained with 2% uranyl acetate and observed under a FEI electron microscope at 80 kV ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed twice with Perm buffer and incubated with Cy3-conjugated anti- mouse antibody (1:800, Jackson ImmunoResearch Labs) for 30 minutes on ice ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... All secondary antibodies were used at a dilution of 1:10,000 (HRP-conjugated goat anti-rabbit, goat anti-mouse, goat anti-chicken, Jackson ImmunoResearch).
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bioRxiv - Cell Biology 2024Quote: ... or peroxidase-coupled goat anti-rabbit antibody (1:10,000, #111-035-046, both Jackson ImmunoResearch Laboratories, West Grove, PA, USA), using the ECL detection system (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 488 anti-rabbit and Alexa Fluor 405 anti-chicken raised in donkey serum (each 1:500; Jackson ImmunoResearch) were applied for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: anti-rabbit (Alexa 594 conjugated donkey, 1:1000, Jackson ImmunoResearch Labs, #711-585-152); anti-rabbit (Alexa 488 conjugated goat ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies (1:250 anti-goat and anti-guinea pig Jackson Immunoresearch, Cat # 705-166-14 and 706-165-148) are diluted in wash solution and slides are incubated 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The antibody binding was visualized by incubation with secondary antibodies conjugated with Cyanine (Cy)-3 (1:400; Jackson ImmunoResearch Laboratories) for 2h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Negative controls were performed using mouse and rabbit sera (1/1000, Jackson ImmunoResearch, 015-000-120 and 011-000-120) as recommended by The Histochemical Society’s Standards of Practice for Validation of Immunohistochemical Assays (Hewitt et al ...
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bioRxiv - Molecular Biology 2024Quote: ... This was followed by incubation in Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:500; Jackson ImmunoResearch) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies for standard immunostaining were used at the following concentrations: goat anti-HRP-Cy5 (1:250, Jackson ImmunoResearch); goat anti-rabbit-Cy3 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary rabbit HRP conjugated antibody was used in 1:10000 dilution and obtained from Jackson ImmunoResearch (West Grove, PA, USA). HA-tagged RIN4a and RIN4b expressed in soybean transgenic roots were detected using monoclonal HA-antibody (Roche Diagnostics ...
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bioRxiv - Immunology 2024Quote: ... 96-well ELISA plates were coated with 1 μg/mL of goat anti-mouse IgG (H+L) (Jackson ImmunoResearch Laboratories) in PBS for 2 hours at 37 °C then overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the embryo was incubated with the secondary antibody goat anti-mouse IgG-Cy3 (1:1’000, Jackson ImmunoResearch 115-165-003) for 48 hours ...