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Citations for Jackson ImmunoResearch :
3351 - 3400 of 4197 citations for Human Eukaryotic Translation Termination Factor 1 ETF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... washed five times for 3 min each with 0.05% Natural Donkey Serum and then incubated for 1 hr with gold-conjugated secondary antibody (donkey; Jackson Immunoresearch) diluted 1:100 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then washed multiple times in PBS and incubated with Alexa488-Streptavidin (1:200; #016-540-084, Jackson ImmunoResearch) or AlexaCy5-Streptavidin (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibodies used are peroxidase AffiniPure donkey anti-rabbit IgG (H+L) (711-035-152, Jackson ImmunoResearch; 1:10,000), peroxidase AffiniPure donkey anti-goat IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... or goat anti-rabbit Alexa Fluor 488 (Cat. No. 111-545-144, 1:250; Jackson ImmunoResearch Laboratories, West Grove, PA). Alternate sections from each experimental condition were incubated in all solutions except the primary antibodies to assess nonspecific staining ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in 1 × TBS (3 × 10 min) before being incubated in a secondary antibody (1:10,000 HRP-conjugated goat anti-rabbit, Jackson ImmunoResearch), in blocking solution described above ...
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bioRxiv - Neuroscience 2020Quote: ... For visualization horseradish peroxidase-conjugated secondary antibodies were used: Goat anti-mouse (1:20000; Jackson ImmunoResearch Laboratories 115-035-062), and Goat anti-rabbit (1:20000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated with Cy3 conjugated secondary antibody (1:1000 dilution, #706-165-148; Jackson ImmunoResearch Laboratories, West Grove, PA) and Alexa Fluor 488 conjugated secondary antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with donkey anti-rabbit IgG H&L secondary antibody (conjugated to fluor-488 or fluor-594, 1:1,000; Jackson ImmunoResearch) at room temperature for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... (5) cultures were incubated in Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:500, Jackson ImmunoResearch, 711-165-152) and Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibody against RFP was detected with a 2-hour incubation in diluted donkey-anti-rabbit-IgG-Cy3 antibody in blocking buffer (1:1,000, Jackson ImmunoResearch). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... were detected with a 2- hour incubation in adequate secondary antibodies diluted in blocking buffer (1:1,000 for donkey-anti-mouse-IgG-Cy5 or donkey-anti-rabbit-IgG-Cy3, Jackson ImmunoResearch). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies (1:10,000) were used: HRP conjugated donkey anti-rabbit secondary antibody (Jackson ImmunoResearch, # 711-035-152) and HRP conjugated donkey anti-mouse secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies used include goat anti-Armenian hamster IgG Alexa Fluor 594 (Jackson ImmunoResearch Laboratories, West Grove, PA; 1:500), goat anti-chicken IgG Alexa Fluor 647 (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Systems Biology 2022Quote: ... Membranes were washed three times with TBS-Tween and incubated at RT for 1 h in secondary antibody (Jackson ImmunoResearch, Peroxidase conjugated Affinipure goat anti-mouse(H+L ...
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bioRxiv - Genetics 2020Quote: ... embryos were incubated overnight with donkey anti-rat HRP secondary antibody at 1:1000 dilution (Jackson ImmunoResearch, 712-036-153) followed by incubation with 488-tyramide reagent at 1:25 dilution made using Dylight 488 NHS ester (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were washed with PBS in a coplin jar for 1 hr and incubated with donkey secondary antibodies (Jackson ImmunoResearch) and 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2019Quote: ... a secondary antibody of either donkey anti-rat or donkey anti-guinea pig Alexa Fluor 488 (1:500, Jackson ImmunoResearch) was applied overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: ... Antii-guinea pig Ig Alexa 647 and anti-mouse Ig Alexa 488 secondary antibodies were used (1:400; Jackson ImmunoResearch). Images were acquired using a LSM 700 confocal microscope (Zeiss).
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bioRxiv - Microbiology 2019Quote: ... The membranes were then incubated with the corresponding horseradish peroxidase (HRP)-conjugated goat anti-guinea pig (1:10,000; Jackson ImmunoResearch). Mouse HRP-conjugated anti-actin mAb (1:35,000 ...
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bioRxiv - Cell Biology 2019Quote: ... co-stained with rabbit anti-GM130 followed by Alexa 647-conjugated donkey anti-rabbit IgG (1:500) in blocking solution with 0.1% saponin and 5% normal donkey serum (Jackson ImmunoResearch). After washing ...
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bioRxiv - Cancer Biology 2019Quote: ... the blots were incubated with anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, 1:10,000 dilution) for 1 hour and washed 4 times ...
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bioRxiv - Cancer Biology 2020Quote: ... and Alexa Fluor 790 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch, Cat: 706-655-148, 1:500) containing DAPI (Sigma ...
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bioRxiv - Physiology 2021Quote: ... After 3 washes and a 1-hour incubation with the secondary anti-goat antibody (3.3 ng/μL, 705605003; Jackson ImmunoResearch) and DAPI (1 μg/μL ...
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bioRxiv - Bioengineering 2019Quote: ... and finally incubated in blocking/permeabilizing buffer containing AffiniPure Fab goat anti-mouse-IgG (1:25; Jackson ImmunoResearch Laboratories, Inc.) for 1-2 hours at room temperature
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bioRxiv - Developmental Biology 2020Quote: ... The blots probed with α-Nrl antibody were detected using 1:5000 goat α-rabbit HRP secondary antibody (Jackson Immunoresearch catalogue #111-005-003 ...
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bioRxiv - Physiology 2020Quote: ... overnight at 4 °C and then with a donkey anti-guinea-pig Cy5-conjugated antibody for 2.5 hours at RT (1:200, Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked again and incubated with secondary antibodies at 1/250 dilution (Alexa Fluor 647, 555 and 488 from Jackson ImmunoResearch) and/or 1/100 dilution of Alexa Fluor 647 Phalloidin (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... (Tomancak et al., 1998)) and subsequently with secondary antibodies Alexa 647 conjugated donkey anti-rat IgG (1:1000; Jackson ImmunoResearch). Ovaries from flies expressing fusion proteins were fixed in 2% PFA and 0.01% Triton X-100 for 15 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Mouse or rabbit secondary antibodies conjugated to Alexa488 or Cy5 (as appropriate) were used at 1/200 each (Jackson ImmunoResearch). Samples were mounted in VectaShield™ and imaging was performed using a Leica SP5 or SP8 confocal microscope ...
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bioRxiv - Immunology 2019Quote: ... coverslips were stained for 1 hour at room temperature with phalloidin-eFluor660 (eBioscience) or donkey anti-mouse-AF594 (Jackson ImmunoResearch), and mounted onto slides with Vectashield Mounting Medium containing DAPI (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.01% Triton X) and incubated with Secondary antibody (1:400 in blocking buffer, Donkey anti-Rb alxaflour 488 Jackson ImmunoResearch) and TMR-Halo-Ligand (2nM ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were used: Cy3-conjugated anti-armenian hamster (1:200; Jackson ImmunoResearch, cat. no. 127-165-160), AlexaFluor 488-conjugated anti-guinea pig (1:200 ...
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bioRxiv - Biochemistry 2020Quote: ... The anti-His blot was then probed with 1:20,000 horseradish peroxidase-conjugated rabbit anti-mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch) in 5% non-fat milk in PBST for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation (2 h) with appropriate fluorescent secondary antibodies (Cy3-or Cy2-coupled fluorescent secondary antibodies, 1:200; Jackson ImmunoResearch Laboratories catalog #715-165-150 and #715-545-150 respectively) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated at 4°C overnight and followed by incubation of 488- and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch, 1:500) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used in this study: Donkey anti-Guinea pig Alexa Fluor 647 1:500 (Jackson Immunoresearch 706-605-148), Donkey anti-Rabbit Alexa Fluor 488 1:1000 (ThermoFisher A-21206) ...
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bioRxiv - Neuroscience 2021Quote: ... and then blocked for 1 hour at room temperature in 5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA)/0.5% TTBS ...
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bioRxiv - Neuroscience 2021Quote: ... brains were left rocking at 4°C for 1-3 nights in 0.1% PBS-T supplemented with blocking agent normal goat serum (Jackson ImmunoResearch) at a 1:20 dilution ...
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bioRxiv - Genetics 2021Quote: ... the slides were incubated with the AlexaFluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:300; Jackson ImmunoResearch) for 30 minutes After a further wash of 15 minutes in PBT ...
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bioRxiv - Genetics 2021Quote: ... all slides were incubated with the fluorescein (FITC)-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (1:150; Jackson ImmunoResearch) for 1 hour at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated with peroxidase-linked anti-mouse or anti-rabbit IgG (1:10,000) (Jackson ImmunoResearch Laboratories, West Grove, PA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed and incubated with secondary antibody conjugated to Alexa Fluor 488 (Jackson Immunoresearch 711-545-150, 1:500) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... After staining with the anti-mouse horse-radish peroxidase (HRP)-labeled secondary antibody at 1:10,000 (cat. #715-035-151, Jackson ImmunoResearch), the blots were imaged using the SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... room temp) in 0.1% PBST containing the appropriate fluorophore-conjugated secondary antibody (AlexaFluor 488 or 594, 1:2000; Jackson ImmunoResearch). Triton X-100 was included to allow secondary antibody to reach all cellular compartments ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated for 1.5 h at RT with HRP-conjugated antibodies diluted in the blocking solution (1:5,000) against mouse (Jackson ImmunoResearch, AB_2340770) and rabbit (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... Both HRP-conjugated goat anti-rabbit-IgG and goat anti-mouse-IgG antibodies (1:5000) were purchased from Jackson ImmunoResearch Laboratories.
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bioRxiv - Neuroscience 2022Quote: ... All secondary antibodies had a 1:500 dilution and stored overnight at 4 °C (Jackson Immunoresearch; West Grove, PA, USA). Sections were then mounted and coverslipped using 50% glycerol in 0.02 M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Cell Biology 2022Quote: ... MilliporeSigma) was applied for 2 h at RT followed by secondary antibody (fluorophore-conjugated IgG, 1:500; Jackson ImmunoResearch Laboratories) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody was also used followed by a donkey anti-rabbit secondary antibody conjugated to AF488 (1:500, Jackson Immunoresearch).
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibodies used were donkey anti-rabbit and donkey anti-goat at 1:5000 dilutions (Jackson ImmunoResearch, West Grove, PA). Blots were developed using either Clarity Western ECL Substrate (Bio-Rad Laboratories ...