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Citations for Jackson ImmunoResearch :
251 - 300 of 359 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Slices were washed 3 times with PBS before they were blocked with 10% Normal Donkey Serum (NDS, Jackson ImmunoResearch, 017-000-121) and 0.4% Triton-X in 1X PBS for one hour at RT ...
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bioRxiv - Physiology 2021Quote: ... followed by a secondary donkey anti-rabbit Ig antibody coupled to Alexa 488 (3 μg/ml, Jackson ImmunoResearch Laboratories, West Grove, PA). A goat anti-Aqp2 antibody (0.2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in PBS solution and incubated for 1hr at room temperature with secondary antibody (1:500 Cy™3 AffiniPure Donkey Anti-Goat IgG, Jackson ImmunoResearch, 705-165-147 ...
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bioRxiv - Neuroscience 2020Quote: Slices were washed 3 times with PBS before they were blocked with 10% Normal Donkey Serum (NDS, Jackson ImmunoResearch, 017-000-121) and 0.4% Triton-X in 1X PBS for one hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were permeabilized and blocked in 0.3% Triton X-100 and 3% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) in PBS for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The day after sections were washed 3 times with PBS and incubated with secondary antibodies (Goat anti-rabbit Cy2, 1:200, Jackson Immunoresearch (JI)/111-225-144 ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBST 3 times (5 min in each time) and incubated with secondary antibodies (mouse anti-Cy3, Jackson Immunoresearch,1:300) for 1h ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were diluted in block and used at 3-4ug/ml for 1 hour at room temperature (Alexa488, Cy3, orAlexa647; Molecular Probes and Jackson ImmunoResearch). Hoechst at 0.2 ug/ml was used to stain DNA for 3 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... cultures were blocked in PBS-3% BSA and subjected to immunofluorescence labeling using primary antibodies followed by incubation with appropriate secondary antibodies conjugated to Alexa-fluor 488 or Cy-3 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Cell Biology 2024Quote: ... Thermo Fisher)] in PBST + 3% BSA for 1 hr followed by either horseradish peroxidase-conjugated polyclonal goat anti-mouse (115–035-146; Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated polyclonal goat anti-rabbit (170-6515 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in 1X PBS then transferred to secondary antibody (Jackson ImmunoResearch Labs: 1:500 in 3% NDS in 1X PBS) overnight at 4C ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121; Jackson ImmunoResearch]) for 2 hours at room temperature and then incubated for 48 hours at 4°C with primary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides stored in PBS were blocked in 5% horse serum then incubated overnight with anti-CENP-C antibody (1:200, gift, W. Earnshaw) before 1 hr incubation with Texas Red labelled anti-rabbit (1:100, Jackson ImmunoResearch Laboratories) secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Extended Data Table 1) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times in PBS and incubated for 60–90 min at room temperature or overnight at 4°C with the Alexa Fluor 488- and 594 and 647-conjugated secondary antibodies (1:500; Thermo Fisher Science or Jackson ImmunoResearch).
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibody overnight at 4°C and secondary antibody (anti-rabbit-Alexa Fluor 647, 1:400, Jackson ImmunoResearch, Inc., West Grove, PA) for 3-4 hours in the dark ...
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bioRxiv - Physiology 2024Quote: ... treated with 1M HCl for 1 hour and incubated overnight at 4°C with anti-BrdU antibody following incubation with Alexa Fluor-488 conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). Slides were mounted in mounting medium for fluorescence with DAPI (Vector Laboratories) ...
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bioRxiv - Plant Biology 2022Quote: ... the seedlings were washed for three times 5 min each and then incubated in the secondary antibody Cy-3-goat anti-mouse IgG (1:200; Jackson Immunoresearch, http://www.jacksonimmuno.com). The imaging was performed on a Nikon laser scanning microscope (Eclipse Ti equipped with Nikon C2 Si laser scanning unit ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed 3× in PBST for 10min each and stained with secondary antibody Goat α-Rabbit-Cy3 (Jackson ImmunoResearch, West Grove, PA) 1:500 in 5% normal goat serum PBST for 2h at RT and then rinsed 3× ...
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bioRxiv - Bioengineering 2020Quote: ... Total mouse IgG against GAA was measured using HRP-goat anti-mouse IgG (subclasses 1 + 2a + 2b + 3) (Jackson Immunoresearch, 115-035-164) and a colorimetric kit (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated with secondary antibody (PTwH, 3% donkey serum, Cy3-conjugated Donkey Anti-Goat, Jackson ImmunoResearch Labs 705-165-147, 1:1000) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were rinsed with PBS (3 × 10 min) and incubated with a secondary antibody solution (1:1000, 711-175-152, Jackson Immunoresearch, Ely, UK)) containing 3 % NDS in PBS for 1.5 h at RT.
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bioRxiv - Neuroscience 2022Quote: ... DRN sections were rinsed in PBS (3 × 10 min) and incubated with a secondary antibody solution (donkey anti rabbit Cy5 (1:1000, 711-175-152, Jackson Immunoresearch, Ely, UK) and goat anti mouse Alexa 555 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were then washed with TBST (3 times for 15 min) and incubated with anti-rabbit-HRP (111-036-045; Jackson ImmunoResearch; 1:5000) secondary antibody ...
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bioRxiv - Genomics 2023Quote: ... 3 times at room temperature and then incubated with secondary antibodies: Cy3 donkey anti-goat (Jackson ImmunoResearch, Cat# 715-165-150, 1:500), Alexa Fluor 488 donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times for 10 minutes in PBS before incubation with the secondary antibody (1:1000, donkey anti-rabbit IgG, Jackson ImmunoResearch, PA, USA) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated with Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1.9 µg/ml; Jackson ImmunoResearch, cat #111-165-003) for standard epifluorescence assays ...
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bioRxiv - Physiology 2023Quote: ... incubated for 90 min at room temperature with a donkey anti-human CyTM 3 antibody (1:500; Jackson ImmunoResearch, Cat #709-165-149) and coverslipped ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed (3 × 5mins in 0.01M PBS) and then incubated with secondary antibody for 2 hr (Jackson ImmunoResearch, 1:200 in block). Following washing (3 × 5mins in 0.01M PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... animals were incubated overnight at 4°C in Alexa Fluor 488 donkey anti-chicken secondary antibody (1:1000, Jackson ImmunoResearch, 703-545-155), Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch, Westgrove, PA; 1:10,000) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2% Triton X-100 and incubated overnight at 4°C with a CY5-conjugated secondary antibody (1:400, goat anti-mouse, Jackson Immunoresearch, Suffolk, UK), washed in 1x PBS and embedded in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (4°C overinight) and then secondary peroxidase-conjugated Goat Anti-Rabbit IgG (Jackson ImmunoResearch 111-035-144) or peroxidase-conjugated Goat Anti-Mouse IgG (Jackson Immuno Research 115-035-174 ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with an anti-IE1/2 antibody for 2 hours at 37°C and an HRP-conjugated rabbit anti-mouse secondary antibody (Jackson ImmunoResearch, Cambridge, UK) for 45 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The monolayers were labeled with a rabbit anti-chlamydial antibody (raised by immunization with C. muridarum EBs) and a goat anti-rabbit IgG conjugated with Cy2 (green, Jackson ImmunoResearch Laboratories, Inc) to visualize chlamydial inclusions while a Hoechst dye (blue ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody against DPF-3 was used at 1:200 dilution and anti-rat secondary antibodies fused to HRP (Jackson ImmunoResearch, 712-035-150) were used at 1:2000 dilution ...
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bioRxiv - Physiology 2020Quote: ... Cells were then rinsed 3 times with PBS and incubated with FITC-Goat Anti-Mouse IgG (Jackson ImmunoResearch Inc.,115-095-003; 1:200) with tetramethylrhodamine (TRITC)-phalloidin (1 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... the embryos were washed 3 times in the staining buffer (30 mins intervals between washes) and labeled with Alexa Fluor 488 Streptavidin (Jackson ImmunoResearch, 016-540-084). Labeled embryos were washed 3 times in the staining buffer (30 minutes between washes ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 45-60 min in blocking solution that consisted of PBS containing 3% Bovine Serum Albumin (Jackson Immunoresearch, 001-000-162) and 3% Donkey Serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... primary staining was followed by Cyanine 3-labeled donkey anti-sheep and a fluorescein isothiocyanate (FITC)-conjugated antibody against either rabbit or mouse antibody (Jackson ImmunoResearch, West Grove, PA). Otherwise ...