Labshake search
Citations for Jackson ImmunoResearch :
2501 - 2550 of 2590 citations for Goat ZAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cover slips were then blocked in 150 μL of blocking solution (5% [w/v] BSA, 5% [v/v] Normal Goat Serum [Jackson ImmunoResearch Laboratories] in 1× PBS) at RT for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed thrice with TBS containing 0.1% Tween-20 followed by incubation with goat anti-mouse IgG HRP-conjugate (Jackson ImmunoResearch, 115-035-003; 1:5000). Membranes were washed thrice with TBS containing 0.1% Tween-20 ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 µL of FACS buffer containing secondary R-PE Goat Anti-Human IgG antibody diluted 1:100 (Jackson ImmunoResearch, cat #109-117-008), and incubated for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Total IgG was measured following 6 washes and a 1-h incubation with 100 μL/well of 1:50,000 HRP-conjugated polyclonal goat anti-human IgG (Jackson ImmunoResearch Labs, West Grove, PA, USA). IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were subsequently incubated at room temperature with Alexa 594-conjugated goat anti-rabbit IgGs (1/500; 90 min; Jackson Immunoresearch Laboratories, West Grove, PA). Images were acquired using a confocal microscope (LSM880 ...
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bioRxiv - Cancer Biology 2023Quote: ... washed thrice with PBS and then stained with goat anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 647 (Jackson ImmunoResearch Labs; West Grove, PA) to monitor for p-STAT-5 in CD8+ OT-I cells by flow cytometry.
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-goat or anti-mouse biotinylated secondary antibodies were incubated for 2 hours at room temperature (1:1000, Jackson ImmunoResearch, West Grove, PA, USA), followed by incubation with VECTASTAIN Elite ABC HRP solution (Vector ...
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bioRxiv - Biochemistry 2023Quote: ... 1:5,000 dilution in ELISA buffer) and peroxidase conjugated goat anti-mouse IgG (cat: 115-035-003, Jackson Immunoresearch, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed twice in 1XPBS for 5’ each and incubated with Goat anti-Rat-Biotin (1:300, in 1XPBS + 1%NGS, Jackson ImmunoResearch Europe Ltd, Ely, UK) at RT for 1h.
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bioRxiv - Immunology 2023Quote: ... and secondaries antibodies Rhodamine RED-X (RRX) rabbit anti-mouse IgM and Alexa Fluor 647 goat anti-mouse IgG (both 10 μg/mL, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... Rhodamine RED-X (RRX) donkey anti-mouse IgM and Alexa Fluor 647 goat anti-mouse IgG (all at 10 μg/mL, Jackson ImmunoResearch, West Grove, PA, USA) were added for 3 hours at RT ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies Rhodamine RED-X (RRX) rabbit anti-mouse IgM and Alexa Fluor 647 goat anti-mouse IgG (both 10 μg/mL, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... treated isolated MCA branches by 1% Triton X-100 (Diamond, Cat# A110694-0500, CHN) with 2.5% Normal Goat Serum (Jackson ImmunoResearch, Cat# 005-000-121, USA) in 1X PBS for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for one hour in a biotinylated secondary antibody (1:600, Biotin-SP AffiniPure Goat Anti-Rabbit IgG, Jackson Immunoresearch Labs; 111-065-003) in 0.4% Triton-X 100 in PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... samples were placed in a secondary antibody solution of Alexa Flour 488 AffiniPure Goat Anti-Rat IgG (1:300, Jackson ImmunoResearch, cat. 112-035-003) and DAPI overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Animals were then placed in a secondary antibody solution of Alexa Flour 488 AffiniPure Goat Anti-Rabbit IgG (1:500, Jackson ImmunoResearch, cat. 111-545-144) for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: The IgG assay utilized a 1:100 dilution of goat anti-human IgG (Fc)-HRP in Conjugate Diluent (HRP-StabilPlus, Jackson Immunoresearch Laboratories, West Grove, PA) was added for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed three times and then incubated 2 h rocking at RT with the secondary antibody goat anti-rabbit Cy3 (Cy3, 1:200, Jackson ImmunoResearch, West Grove, PA, USA). Brain slices were finally mounted on microscope slides using DAPI Fluoromount-G (SouthernBiotec) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 15 min in a 100 mm Petri-dish coated with goat anti-rabbit IgG antibody (1:400, Jackson ImmunoResearch Inc, West Grove, PA). After shaking and washing to remove unattached cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunohistochemistry for dsRed was performed with a 7 minute −20°C 100% acetone target retrieval and blocking in 2% normal goat serum (Jackson ImmunoResearch, cat. no. 005-000-121). Primary antibodies include rabbit anti-mCherry (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed three times for 20 min each in PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch, AB_2338680, 1:200 v/v) and goat anti-rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-human IgM (Jackson Immunoresearch Affinipure; 0.6-75 ug/mL), or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure ...
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bioRxiv - Systems Biology 2019Quote: ... Antibody binding signal was detected using Alexa Fluor 488 and 594 goat anti-mouse IgG secondary antibodies (Jackson ImmunoResearch, #115-545-003 and #115-585-003). In brief ...
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bioRxiv - Cell Biology 2021Quote: ... and Cdc42 were done under conventional bead-precipitation and western blot protocols using goat anti-mouse alkaline phosphatase secondary antibodies (Jackson ImmunoResearch, Philadelphia, PA, USA, 1:5000) and a colorimetric detection kit (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... 0.04% NaN3 with 1:500 Alexa Fluor 488/594-conjugated donkey anti-rabbit/goat/mouse (Jackson ImmunoResearch, 711-585-152, 705-585-147, 715-545-151) for 4 hours at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were rinsed with TBST buffer three times and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) in a blocking solution at RT for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... sections were thoroughly washed before being incubated 90 min with the biotinylated secondary antibody goat-anti rabbit (1:300; Jackson ImmunoResearch, catalogue number 111-066-046) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 h at room temperature in a solution containing a mixture of goat-Cy3 anti-rabbit (1:500, Jackson ImmunoResearch Laboratories, Cat. #111–165–144), goat anti-chicken Alexa 647 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 3X with FACS buffer and stained with secondary antibody Cy3-conjugated Goat Anti-mouse IgG (Jackson ImmunoResearch, 2 μg/mL in FACS buffer) for 1 hour at RT ...
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bioRxiv - Immunology 2019Quote: A20 D1.3 cells were activated with a mixture of 6 nm colloidal-gold conjugated goat anti-Mouse IgM (Jackson ImmunoResearch, 115-195-075; 1:650 dilution) and 20 μg/ml Alexa Fluor 647 labelled donkey anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... with 20 μg/ml Affinipure Fab fragment goat anti-mouse IgG (H+L) (catalog number 115-007-003, Jackson ImmunoResearch Laboratories, Inc. West Grove, PA, USA). The following primary antibodies were used ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then washed 3x with PBS and stained with the secondary antibodies anti-goat Alexa Fluor® 488 (Jackson ImmunoResearch, #705-546-147, 1:500), anti-rabbit Alexa Fluor® 555 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human serum IgA (α-chain specific) at 0.3 μg/mL or goat anti-human IgG (γ-chain specific) at 5 μg/mL (all from Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Microtiter plates were sealed and stored overnight at 2-8°C ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently incubated for 2 h at room temperature in goat-Cy3 anti-rabbit purified secondary antibody (1:500, Jackson ImmunoResearch Laboratories, Cat. #111-165-144) goat anti-chicken Alexa Fluor® 647 secondary antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated with secondary antibodies conjugated to alkaline phosphatase anti-IgM or anti-IgG diluted 1:2000 (rabbit anti-mouse IgM, Jackson ImmunoResearch, goat anti-mouse IgG, SouthernBiotech). Substrate NBT/BCIP (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... 0.04% NaN3 with 1:500 Alexa Fluor 488/594-conjugated donkey anti-rabbit/goat/mouse (Jackson ImmunoResearch, 711-585-152, 705-585-147, 715-545-151) for 4 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in PBS solution and incubated for 1hr at room temperature with secondary antibody (1:500 Cy™3 AffiniPure Donkey Anti-Goat IgG, Jackson ImmunoResearch, 705-165-147; 1:500 Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG, Jackson ImmunoResearch, 711-545-152). Finally ...
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bioRxiv - Physiology 2023Quote: ... in PBS-GT were followed by the incubation with secondary antibodies (1:500; Cy3 anti-goat IgG and Cy5 anti-rabbit IgG; Jackson ImmunoResearch Europe Ltd., Cambridge House, UK) for 7 days at RT.
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bioRxiv - Neuroscience 2024Quote: ... was used to detect artery staining and Alexa Fluor® 647-AffiniPure Fab Fragment Donkey Anti-Goat IgG (H+L) (Jackson ImmunoResearch laboratories; 705-607-003) was utilized to detect pericyte staining ...
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bioRxiv - Immunology 2023Quote: ... or unconjugated anti-human CCL19 (polyclonal, R&D AF361-SP, 1:200) that was detected with anti-goat IgG A488 (Jackson Immunoresearch 705-545-003, 1:1000) and unconjugated anti-human PDPN (Clone NZ-1.3 ...
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bioRxiv - Microbiology 2023Quote: ... for visualizing DNA and a rabbit anti-chlamydial organisms (unpublished data) plus a goat anti-rabbit IgG conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for visualizing chlamydial inclusions ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with the same buffer (3 × 10 min) and incubated for 1 hour either with Horseradish-Peroxydase (HRP) goat anti-rabbit IgG (1:3000, Jackson Immunoresearch, Cambridge, UK, #111-035-144) or with HRP Goat anti-mouse IgG (1:5000 ...
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bioRxiv - Genetics 2023Quote: ... We washed brains three times with PAXD for 10 minutes and stained them with Goat anti-Mouse lgG-AlexaFluor488 (1:100) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for 4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed three times for 10 min in PBS and incubated for 24 h at 4°C in secondary antibodies (donkey anti-rabbit Alexa Fluor 488, 1:250, Thermo Fisher Scientific, cat# A-21206, RRID:AB_2535792; donkey anti-goat Cy3, 1:1000, Jackson ImmunoResearch, cat# 705-165-147, RRID: AB_2307351) diluted in PBS-T with 1 % NDS ...
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bioRxiv - Neuroscience 2023Quote: ... 0.04% NaN3 with 1:500 Alexa Fluor 488/594-conjugated donkey anti-rabbit/goat/mouse (Jackson ImmunoResearch, 711-585-152, 705-585-147, 715-545-151) for 4 hours at RT ...
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bioRxiv - Bioengineering 2023Quote: ... cells were then incubated with a corresponding secondary antibody conjugated at room temperature for 1 h (anti-rabbit Alexa Fluor 488, anti-mouse Alexa Fluor 647, anti-goat Cy™3, Jackson ImmunoResearch Inc., West Grove, PA) in 1% BSA in PBS ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG-FITC (Cat: 111-095-045) or goat anti-rabbit IgG-Rhodamine Red™-X (Cat: 111-295-045) (Jackson ImmunoResearch Laboratories, Westgrove, PA USA) were diluted 1:500 in PBST and applied for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The washed ELISA plates were incubated with a 1:1 mixture of mouse anti-M13 monoclonal antibody (GE, 1:5,000 dilution in ELISA buffer) and peroxidase-conjugated goat anti-mouse IgG (Jackson Immunoresearch, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... mouse IgG1 monoclonal anti-HA primary antibody at 1:1000 (Covance, Princeton, NJ, USA) with corresponding Rhodamine Red-conjugated goat anti-mouse IgG1 secondary antibody at 1:100 (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) or Alexa Fluor® 546 goat anti-mouse (Jackson ImmunoResearch Laboratories ...