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Citations for Jackson ImmunoResearch :
201 - 250 of 4345 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were permeabilized and blocked in 0.3% Triton X-100 and 3% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) in PBS for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... cultures were blocked in PBS-3% BSA and subjected to immunofluorescence labeling using primary antibodies followed by incubation with appropriate secondary antibodies conjugated to Alexa-fluor 488 or Cy-3 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121; Jackson ImmunoResearch]) for 2 hours at room temperature and then incubated for 48 hours at 4°C with primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 mM EDTA) and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003 ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed 3× in PBST for 10min each and stained with secondary antibody Goat α-Rabbit-Cy3 (Jackson ImmunoResearch, West Grove, PA) 1:500 in 5% normal goat serum PBST for 2h at RT and then rinsed 3× ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and secondary antibodies (Jackson ImmunoResearch, 715-165-150) at 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and secondary antibodies (Jackson ImmunoResearch, 715-165-150) at 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated with Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1.9 µg/ml; Jackson ImmunoResearch, cat #111-165-003) for standard epifluorescence assays ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2–4 h with a mixture of fluorophore-conjugated secondary antibodies (all raised in donkey, 1:500, Jackson Immunoresearch): Anti-guinea pig IgG (DyLight 488 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 days at 4°C (iii) 1:400 of biotinylated anti-goat IgG (Jackson ImmunoResearch, catalog 705-065-147), 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... the embryos were washed 3 times in the staining buffer (30 mins intervals between washes) and labeled with Alexa Fluor 488 Streptavidin (Jackson ImmunoResearch, 016-540-084). Labeled embryos were washed 3 times in the staining buffer (30 minutes between washes ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 45-60 min in blocking solution that consisted of PBS containing 3% Bovine Serum Albumin (Jackson Immunoresearch, 001-000-162) and 3% Donkey Serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... primary staining was followed by Cyanine 3-labeled donkey anti-sheep and a fluorescein isothiocyanate (FITC)-conjugated antibody against either rabbit or mouse antibody (Jackson ImmunoResearch, West Grove, PA). Otherwise ...
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bioRxiv - Developmental Biology 2021Quote: ... The chips were then washed twice with PBS, and the cells were permeabilized with blocking solution (PBS 0.1% Tryton-X, 3% of Donkey Serum (Jackson Immunoresearch Cat#017-000-121)) for 45 minutes at RT ...
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bioRxiv - Systems Biology 2023Quote: The following secondary antibodies were used for staining: Cy™3 AffiniPure Fab Fragment Goat Anti-Mouse IgG2a (Jackson ImmunoResearch, Cat# 115-167-186), Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were first washed in PBS 5mins for 3 times and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Immunology 2024Quote: ... excess primary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by incubation of donkey anti-mouse Cy3 secondary antibody (Jackson Immunoresearch, Cat# 715-167-003) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Clone SMI-31 reacts with a phosphorylated epitope of neurofilament in mammals and neurofilament 220 in squid51–53 .The secondary Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (Cat#: 115-545-003) and Cy™3 AffiniPure Donkey Anti-Mouse IgG (Cat#: 715-165-151) were purchased from Jackson ImmunoResearch (West Grove, PA) and employed at 1:500 dilutions.
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bioRxiv - Biochemistry 2021Quote: ... F(ab’)2 fragment specific (Jackson ImmunoResearch: 1:1000). Cells were washed 3x with PBS + 3% BSA and fluorescence was quantified using a CytoFLEX (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... then with secondary AlexA 488-conjugated antibody (Jackson ImmunoResearch Laboratories, dil. 1/500, 1 h at 4°C). FACS analysis of cell fluorescence was performed on a MACSQuant Analyzer (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked for 1 hour at 4°C in normal donkey serum (1:20; Jackson Immunoresearch; RRID: AB_2337258). Primary antibodies used were chicken anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... selected coronal sections were rinsed three times in TBS and incubated in blocking solution (3% normal donkey serum, Jackson Immunoresearch AB2337258; 0.3% Triton X-100 in TBS) at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with primary antibodies (see Key Resources Table) overnight and subsequent incubated with secondary antibodies labeled with Alexa Fluor 488- (Jackson ImmunoResearch, 115-545-003, 3 μg/mL) or Cy3- (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... in 1% (w/v) BSA 2% donkey serum (Jackson Immunoresearch, West Grove ...
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bioRxiv - Neuroscience 2021Quote: ... (4) incubated in secondary antibody (1:500 703-545-155, Jackson ImmunoResearch) and lectin (for labeling blood vessels ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:200 and donkey F(ab’)2 anti-human IgM Fc5μ at 1:400 dilution (1 mg/ml, Jackson ImmunoResearch Laboratories, Inc.) was used as the secondary antibody for detection.
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and Alexa488-conjugated anti-chicken secondary antibodies (Jackson ImmunoResearch, 715-165-150 and 711-225-152) at 1:500 and 1:1000 dilution in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse unconjugated F(ab’)2 fragments (1:300, Jackson ImmunoResearch), were added in the blocking step ...
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bioRxiv - Immunology 2021Quote: ... primary antibodies were incubated overnight at 4°C followed by HRP-conjugated secondary antibodies for 1 hour at room temperature (1:10000, Jackson ImmunoResearch). Proteins were visualized with chemiluminescent ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Developmental Biology 2021Quote: ... and St18 (1:1000) were incubated in 5% NDS/PBS overnight at 4°C and secondary antibodies (Jackson Immunoresearch, 1:1000) diluted in 0.01% Triton X-100/PBS for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
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bioRxiv - Neuroscience 2022Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary:secondary molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Physiology 2020Quote: ... A 4-hour incubation of 1:250 secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA) followed ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2020Quote: ... and then probed with anti-α-tubulin (YL1/2 1:10,000) followed by Cy5 anti-rat secondary antibody (Jackson ImmunoResearch, 1:2000). To quantify the tubulin signals ...
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bioRxiv - Immunology 2021Quote: ... anti-IgM F(ab’)2 (1 ug/ml, Jackson Immunoresearch, Cat#115-006-020), IL-21 (50 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary : secondary molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2020Quote: ... then with anti-mouse horseradish peroxidase-conjugated antibodies (1/3000; 2 h; Jackson ImmunoResearch) and revealed with ECL substrate (Lumi-Light Western Blotting Substrate ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 20µL of FACS buffer containing 1-2% normal mouse serum (Jackson ImmunoResearch) and 1-2% mouse gamma globulin ...
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bioRxiv - Immunology 2023Quote: ... serum and 1% AffiniPure F(ab′)2 fragment donkey anti-mouse IgG (Jackson ImmunoResearch). Primary antibodies were diluted in IF buffer at the dilutions detailed below (Supplementary table 5 ...
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bioRxiv - Immunology 2024Quote: ... 1:1000 diluted Alkaline phosphatase conjugated anti-human F(ab’)2 antibodies (Jackson ImmunoResearch) were incubated in the plate for 1 hr and then washed ...
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bioRxiv - Neuroscience 2023Quote: ... followed by fluorescently conjugated secondary antibodies in 1:2 (primary:secondary) molar ratios (Jackson ImmunoResearch). After active labeling ...