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Citations for Jackson ImmunoResearch :
201 - 247 of 247 citations for 3 22 Dihydroxyolean 12 En 29 Oic Acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121; Jackson ImmunoResearch]) for 2 hours at room temperature and then incubated for 48 hours at 4°C with primary antibodies ...
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bioRxiv - Physiology 2024Quote: ... slices were incubated at 4°C for 48–72 h in pSTAT3 antibodies (1:3000) in 3% normal donkey serum (Jackson ImmunoResearch Labs) with PBT and 0.02% sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in 1X PBS then transferred to secondary antibody (Jackson ImmunoResearch Labs: 1:500 in 3% NDS in 1X PBS) overnight at 4C ...
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bioRxiv - Plant Biology 2022Quote: ... the seedlings were washed for three times 5 min each and then incubated in the secondary antibody Cy-3-goat anti-mouse IgG (1:200; Jackson Immunoresearch, http://www.jacksonimmuno.com). The imaging was performed on a Nikon laser scanning microscope (Eclipse Ti equipped with Nikon C2 Si laser scanning unit ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed 3× in PBST for 10min each and stained with secondary antibody Goat α-Rabbit-Cy3 (Jackson ImmunoResearch, West Grove, PA) 1:500 in 5% normal goat serum PBST for 2h at RT and then rinsed 3× ...
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bioRxiv - Bioengineering 2020Quote: ... Total mouse IgG against GAA was measured using HRP-goat anti-mouse IgG (subclasses 1 + 2a + 2b + 3) (Jackson Immunoresearch, 115-035-164) and a colorimetric kit (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated with secondary antibody (PTwH, 3% donkey serum, Cy3-conjugated Donkey Anti-Goat, Jackson ImmunoResearch Labs 705-165-147, 1:1000) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times for 10 minutes in PBS before incubation with the secondary antibody (1:1000, donkey anti-rabbit IgG, Jackson ImmunoResearch, PA, USA) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated with Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1.9 µg/ml; Jackson ImmunoResearch, cat #111-165-003) for standard epifluorescence assays ...
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bioRxiv - Physiology 2023Quote: ... incubated for 90 min at room temperature with a donkey anti-human CyTM 3 antibody (1:500; Jackson ImmunoResearch, Cat #709-165-149) and coverslipped ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were rinsed with PBS (3 × 10 min) and incubated with a secondary antibody solution (1:1000, 711-175-152, Jackson Immunoresearch, Ely, UK)) containing 3 % NDS in PBS for 1.5 h at RT.
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bioRxiv - Neuroscience 2022Quote: ... DRN sections were rinsed in PBS (3 × 10 min) and incubated with a secondary antibody solution (donkey anti rabbit Cy5 (1:1000, 711-175-152, Jackson Immunoresearch, Ely, UK) and goat anti mouse Alexa 555 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by further rinses in 0.01 M PBS (3 × 10min) and a 12h incubation at 4 °C in biotin-streptavidin conjugated AffiniPure IgG (anti-rabbit-streptavidin Jackson Immunoresearch, 1:200), followed by three washes with 0.01 M PBS ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were then washed with TBST (3 times for 15 min) and incubated with anti-rabbit-HRP (111-036-045; Jackson ImmunoResearch; 1:5000) secondary antibody ...
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bioRxiv - Genomics 2023Quote: ... 3 times at room temperature and then incubated with secondary antibodies: Cy3 donkey anti-goat (Jackson ImmunoResearch, Cat# 715-165-150, 1:500), Alexa Fluor 488 donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed (3 × 5mins in 0.01M PBS) and then incubated with secondary antibody for 2 hr (Jackson ImmunoResearch, 1:200 in block). Following washing (3 × 5mins in 0.01M PBS) ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody against DPF-3 was used at 1:200 dilution and anti-rat secondary antibodies fused to HRP (Jackson ImmunoResearch, 712-035-150) were used at 1:2000 dilution ...
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bioRxiv - Physiology 2020Quote: ... Cells were then rinsed 3 times with PBS and incubated with FITC-Goat Anti-Mouse IgG (Jackson ImmunoResearch Inc.,115-095-003; 1:200) with tetramethylrhodamine (TRITC)-phalloidin (1 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... the embryos were washed 3 times in the staining buffer (30 mins intervals between washes) and labeled with Alexa Fluor 488 Streptavidin (Jackson ImmunoResearch, 016-540-084). Labeled embryos were washed 3 times in the staining buffer (30 minutes between washes ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 45-60 min in blocking solution that consisted of PBS containing 3% Bovine Serum Albumin (Jackson Immunoresearch, 001-000-162) and 3% Donkey Serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... primary staining was followed by Cyanine 3-labeled donkey anti-sheep and a fluorescein isothiocyanate (FITC)-conjugated antibody against either rabbit or mouse antibody (Jackson ImmunoResearch, West Grove, PA). Otherwise ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed 3 x 5 min with PBS and the secondary antibodies – donkey-anti-mouse Cy3 (1:500, Jackson ImmunoResearch, 715-165-151) and goat-anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... The chips were then washed twice with PBS, and the cells were permeabilized with blocking solution (PBS 0.1% Tryton-X, 3% of Donkey Serum (Jackson Immunoresearch Cat#017-000-121)) for 45 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Retinae were then washed in 0.1% PBST for 3 × 10 minutes at room temperature and incubated with goat-anti mouse Cy3 (1:250, Jackson ImmunoResearch Labs, 115-165-166) or goat-anti rabbit Alexa 647 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were first washed in PBS 5mins for 3 times and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Systems Biology 2023Quote: The following secondary antibodies were used for staining: Cy™3 AffiniPure Fab Fragment Goat Anti-Mouse IgG2a (Jackson ImmunoResearch, Cat# 115-167-186), Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with H2.3 primary rabbit polyclonal anti-HSulf-2 antibody (dil. 1/1000) followed by fluorescent-conjugated secondary anti-rabbit antibody (Jackson ImmunoResearch Laboratories, dil. 1/500). All the incubations were performed for 2 h at 4 °C in TBS ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed and then incubated 3 h with a secondary antibodies cocktail of Cy3 donkey anti-rabbit (1:600; Jackson ImmunoResearch; #711-165-152, AB_2307443) and Cy2 donkey anti-chicken (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Clone SMI-31 reacts with a phosphorylated epitope of neurofilament in mammals and neurofilament 220 in squid51–53 .The secondary Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (Cat#: 115-545-003) and Cy™3 AffiniPure Donkey Anti-Mouse IgG (Cat#: 715-165-151) were purchased from Jackson ImmunoResearch (West Grove, PA) and employed at 1:500 dilutions.
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bioRxiv - Immunology 2024Quote: ... excess primary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by incubation of donkey anti-mouse Cy3 secondary antibody (Jackson Immunoresearch, Cat# 715-167-003) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed three times for 20 min each in PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch, AB_2338680, 1:200 v/v) and goat anti-rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with the same buffer (3 × 10 min) and incubated for 1 hour either with Horseradish-Peroxydase (HRP) goat anti-rabbit IgG (1:3000, Jackson Immunoresearch, Cambridge, UK, #111-035-144) or with HRP Goat anti-mouse IgG (1:5000 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were then incubated with a corresponding secondary antibody conjugated at room temperature for 1 h (anti-rabbit Alexa Fluor 488, anti-mouse Alexa Fluor 647, anti-goat Cy™3, Jackson ImmunoResearch Inc., West Grove, PA) in 1% BSA in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The α-actin secondary antibody was a Cy-3 conjugated AffiniPure donkey anti-mouse at a dilution of 1:500 (Jackson ImmunoResearch Laboratories Inc. #715-166-150). Bands for p-ERK ...
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bioRxiv - Neuroscience 2020Quote: ... selected coronal sections were rinsed three times in TBS and incubated in blocking solution (3% normal donkey serum, Jackson Immunoresearch AB2337258; 0.3% Triton X-100 in TBS) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with primary antibodies (see Key Resources Table) overnight and subsequent incubated with secondary antibodies labeled with Alexa Fluor 488- (Jackson ImmunoResearch, 115-545-003, 3 μg/mL) or Cy3- (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were washed 3 times with PBS and then incubated with 1:200 Alexa Fluor 488 donkey anti-mouse IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA; 715-545-151), 1:200 Fluorescein (Cy3 ...