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Citations for Jackson ImmunoResearch :
2101 - 2150 of 2281 citations for Recombinant Mouse Icam1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Fascin regulates protrusions and delamination to mediate invasive, collective cell migration in vivobioRxiv - Cell Biology 2019Quote: ... AF568::goat anti-rabbit (Thermo Fischer Scientific) and AF647::goat anti-mouse and AF488::goat anti-rat (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). AF647- ...
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bioRxiv - Physiology 2020Quote: ... Cells were then rinsed 3 times with PBS and incubated with FITC-Goat Anti-Mouse IgG (Jackson ImmunoResearch Inc.,115-095-003; 1:200) with tetramethylrhodamine (TRITC)-phalloidin (1 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Then membranes were rinsed in Tris-Buffer (pH =7.4) with 0.1 % of Tween-20 and incubated with HRP-conjugated secondary antibodies – anti-mouse IgG (Jackson Immunoresearch, 715-005-150, Ely, UK) or with anti-rabbit IGG (Abcam ...
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bioRxiv - Biophysics 2019Quote: ... The primary antibody was detected using a secondary goat anti mouse IgG Fcγ subclass 2b antibody (Jackson ImmunoResearch Labs Cat# 115-005-207, RRID:AB_2338463). The secondary antibody was conjugated to Alexa Fluor 532 using methods described above.
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed with PBS and then stained with secondary antibodies donkey anti-rabbit-AF488 and donkey anti-mouse-AF647 (1:1000, Jackson ImmunoResearch, West Grove, Pennsylvania) for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... primary staining was followed by Cyanine 3-labeled donkey anti-sheep and a fluorescein isothiocyanate (FITC)-conjugated antibody against either rabbit or mouse antibody (Jackson ImmunoResearch, West Grove, PA). Otherwise ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed 3 x 5 min with PBS and the secondary antibodies – donkey-anti-mouse Cy3 (1:500, Jackson ImmunoResearch, 715-165-151) and goat-anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Biophysics 2020Quote: ... DNA-conjugated secondary antibody staining was performed by incubating the sample with a donkey anti-mouse secondary fragment antibody (Jackson ImmunoResearch, 715-007-003) at a 1:100 dilution in 5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... In both cases secondary staining was done by 1 h treatment at room temperature with a mix of donkey anti-mouse DNA-conjugated secondary fragment antibody (Jackson ImmunoResearch, 715-007-003) and donkey anti-rabbit DNA-conjugated secondary fragment antibody (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202; anti-rabbit-Alexa568, Invitrogen A10042; anti-sheep-Cy3, Jackson ImmunoResearch 713-165-147). Coverslips were mounted in FluoromountG (SouthernBiotech ...
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bioRxiv - Cell Biology 2021Quote: ... The embryos were washed twice in 4X SSC / 2% BSA / 0.05% Tween20 before and after incubation with the secondary antibody (mouse anti-sheep Ig coupled to FITC, 1/200, 713-095-147, Jackson ImmunoResearch Laboratories Inc., USA) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody staining was followed by washing steps and incubation with antibodies against mouse labeled with Cy3 (Jackson Immunoresearch, #115-165-146, 1:500), against rabbit labeled with Alexa Fluor 488 (ThermoFischer ...
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bioRxiv - Molecular Biology 2022Quote: ... by wet transfer and imaging was done using X-ray film (FujiFilm) to detect signal from HRP-conjugated anti-mouse secondary antibody (1:7500, Jackson ImmunoResearch, #111-035-003) incubated with ECL substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... immunoprecipitates were resuspended in 40 µl of Laemmli buffer and TOPOVIBL immunoprecipitation was assessed by western blotting with a homemade affinity-purified anti-TOPOVIBL (1/1000) antibody followed by an anti-rabbit LC mouse monoclonal secondary antibody (Jackson ImmunoResearch 211-032-171).
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bioRxiv - Neuroscience 2022Quote: ... The following day the sections were rinsed with 0.1M PBS and incubated with the appropriate fluorescent secondary antibodies (anti-mouse or anti-rabbit 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were used at a 1:200 dilution for 30 minutes at room temperature in the dark and include AlexaFluor647 AffiniPure donkey anti-mouse IgG (Jackson ImmunoResearch, 715-605-151), Rhodamine RedX (RRX ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed with PBS and then incubated for 3h at 22–25 °C with Cy3-conjugated anti-mouse (Jackson ImmunoResearch, 715-165-150) at 1:200 dilution in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with PV antibody (Swant, #PV235; 1/50 dilution; anti-mouse IgG1 secondary Fab fragment conjugated with Cy3 (Jackson ImmunoResearch, #115-167-185)) ...
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bioRxiv - Neuroscience 2021Quote: ... Retinae were then washed in 0.1% PBST for 3 × 10 minutes at room temperature and incubated with goat-anti mouse Cy3 (1:250, Jackson ImmunoResearch Labs, 115-165-166) or goat-anti rabbit Alexa 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was washed (5 × 5 min) and incubated in Cy3-conjugated donkey anti-mouse (1:500 in PBS; Jackson Immunoresearch, West Grove, PA) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... the plates were washed five times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody was rabbit monoclonal anti-mouse caveolin-1 (1:100) and Alexa Fluor® 647 Fab1 fragment goat anti-rabbit (Jackson Immunoresearch; 1:100) was used as the secondary to further improve spatial resolution.
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bioRxiv - Neuroscience 2019Quote: ... Sections were subsequently incubated at room temperature with Rhodamine-conjugated goat anti-mouse IgG1 (1/200; 90 min; Jackson Immunoresearch Laboratories, West Grove, PA). Sections were mounted in ProLong® Gold and imaged as for EYFP-labelled interneuron distribution ...
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bioRxiv - Immunology 2019Quote: ... The cells were then placed on ice and labeled for 5 min with 1 ng/ml Cy3-conjugated anti-mouse IgM Fab fragments (Jackson ImmunoResearch, #115-167-020), 100 ng/ml Cy3-conjugated Fab fragments from the 11-26c rat anti-mouse IgD monoclonal antibody (ATCC ...
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bioRxiv - Developmental Biology 2019Quote: ... after several rinses in TBS corneas were blocked with goat anti-mouse IgG Fab fragments (Jackson ImmunoResearch; 0.13 mg/ml; 1 h; room temperature) prior to overnight incubation at 4°C in mouse anti-ZO-1 (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Detection was done with either HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Jackson ImmunoResearch cat#211-032-171, 111-035-144), followed by enhanced chemiluminescence (EZ-ECL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The membrane was washed three times with TBST for 10 min and incubated for 1 hour with 1:10,000 diluted peroxidase-conjugated anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA, USA) in 5% skim milk in TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were stained using a Biotin-SP (long spacer) AffiniPure Fab Fragment Goat Anti-Mouse IgG fragment-specific antibody (catalogue 115-066-072; Jackson Immunoresearch, Stratech Scientific Limited) followed by Streptavidin-APC (catalogue 405207 ...
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bioRxiv - Physiology 2021Quote: ... The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500, #115-545-205, Jackson Immunoresearch, West Grove, PA) for 1 hour at room temp ...
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bioRxiv - Microbiology 2020Quote: ... Beads were then washed and incubated with anti-rabbit FITC (1:100, KPL, 02-15-16) or anti-mouse APC (1:50, Jackson ImmunoResearch, 115-135-164) secondary antibodies for 1 hour at room temperature with gentle shaking ...
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bioRxiv - Biophysics 2020Quote: ... blots were washed in 1X TBS-T buffer and incubated with respective horseradish peroxidase (HRP) conjugated secondary Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 715-035-150) or Anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were washed in PBS + 0.3% triton X-100 for 2 h at RT then incubated in cross-adsorbed Alexa-594 anti-rat and Alexa-488 anti-mouse secondary antibodies (Jackson ImmunoResearch, both 1:400) for 48 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in a PBS solution containing the biotinylated secondary antibody (Biotinylated goat anti-mouse, #115-065-166, Jackson Immunoresearch, diluted 1/200) for 1 hour at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Three washes of 5 minutes each on rotator were done using TBSt before and after the 1h incubation of the membranes with 1:1000 goat anti-mouse-HRP (Jackson Immunoresearch, 115-035-003) in 2%SM in TBSt ...
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bioRxiv - Cell Biology 2021Quote: The following secondary antibodies were conjugated in house using previously described protocol (Dempsey et al., 2011): goat anti-mouse (Jackson ImmunoResearch 715-005-151)-CF568 (Biotium 92131) ...
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bioRxiv - Immunology 2021Quote: ... plates were washed five times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Developmental Biology 2021Quote: ... Then specimens were incubated with 1.5 μl/ml Goat Anti-Mouse IgG antibodies labeled with DyLight 488 (1.5 mg/ml; Jackson ImmunoResearch Cat #115-486-003, USA) in the blocking solution overnight at +4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch, West Grove, PA). Signals were detected using Millipore Immobilon Western Chemiluminescent HRP Substrate (Merk Darmstadt ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (Jackson ImmunoResearch Cat# 715-586-151, RRID:AB_2340858) antibody solution ...
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bioRxiv - Immunology 2022Quote: ... The sections were then washed in distilled water followed by wash buffer and incubated with alkaline phosphatase conjugated donkey anti-mouse and horse radish peroxidase conjugated donkey anti-rabbit secondary antibodies (both Jackson ImmunoResearch, West Grove, PA) at 1:100 dilution (prepared in tris buffered saline with 0.5% bovine serum albumin ...
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bioRxiv - Cancer Biology 2022Quote: ... Frozen VQ- D2 donor cells were gently thawed at 37°C before being washed twice in PBS containing 2% mouse serum (Jackson ImmunoResearch, #015-000-120). Donor cells were then resuspended in 200 μl of PBS containing 2% mouse serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were washed three times with phosphate-buffered saline (PBS) and then incubated with anti-mouse or anti-rabbit Cy3 conjugated secondary antibodies (Jackson ImmunoResearch, dilution 1:1000) in antibody buffer for one hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse tissue sections to be incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
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bioRxiv - Molecular Biology 2023Quote: ... then for one hour with a horse-radish peroxidase-conjugated goat anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA). After washing ...
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bioRxiv - Microbiology 2024Quote: ... Salmonella cultures were grown overnight at 37 °C shaking at 225 rpm or 250 rpm followed by resuspension in phosphate-buffered saline and opsonization in a suspension of DMEM with non-heat inactivated fetal bovine serum and normal mouse serum (Jackson ImmunoResearch, 015-000-120) for 20 minutes.
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were rinsed with PBST and subsequently incubated with secondary goat anti-rabbit-HRP or anti-mouse-HRP antibody (1:4000, Jackson ImmunoResearch, West Grove, USA) in 5% skim milk at room temperature for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... wells were incubated with 50 μl of 1:2000 diluted horseradish peroxidase-conjugated goat anti-mouse IgG Fc antibody (Jackson ImmunoResearch,115-035-008) in 4% blocking buffer for 1.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-or 488-conjugated donkey polyclonal anti-rabbit or anti-mouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature ...