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Citations for Jackson ImmunoResearch :
2101 - 2150 of 2243 citations for Mouse NRAP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch, West Grove, PA). Signals were detected using Millipore Immobilon Western Chemiluminescent HRP Substrate (Merk Darmstadt ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (Jackson ImmunoResearch Cat# 715-586-151, RRID:AB_2340858) antibody solution ...
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bioRxiv - Immunology 2022Quote: ... The sections were then washed in distilled water followed by wash buffer and incubated with alkaline phosphatase conjugated donkey anti-mouse and horse radish peroxidase conjugated donkey anti-rabbit secondary antibodies (both Jackson ImmunoResearch, West Grove, PA) at 1:100 dilution (prepared in tris buffered saline with 0.5% bovine serum albumin ...
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bioRxiv - Cancer Biology 2022Quote: ... Frozen VQ- D2 donor cells were gently thawed at 37°C before being washed twice in PBS containing 2% mouse serum (Jackson ImmunoResearch, #015-000-120). Donor cells were then resuspended in 200 μl of PBS containing 2% mouse serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were washed three times with phosphate-buffered saline (PBS) and then incubated with anti-mouse or anti-rabbit Cy3 conjugated secondary antibodies (Jackson ImmunoResearch, dilution 1:1000) in antibody buffer for one hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were rinsed with PBST and subsequently incubated with secondary goat anti-rabbit-HRP or anti-mouse-HRP antibody (1:4000, Jackson ImmunoResearch, West Grove, USA) in 5% skim milk at room temperature for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse tissue sections to be incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE and were subjected to Western blot analysis with mouse monoclonal anti-Biotin antibody (Jackson Immunoresearch, West Grove, PA) and chicken polyclonal anti-GFP antibody (Aves Labs ...
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bioRxiv - Bioengineering 2024Quote: ... wells were incubated with 50 μl of 1:2000 diluted horseradish peroxidase-conjugated goat anti-mouse IgG Fc antibody (Jackson ImmunoResearch,115-035-008) in 4% blocking buffer for 1.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-or 488-conjugated donkey polyclonal anti-rabbit or anti-mouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Salmonella cultures were grown overnight at 37 °C shaking at 225 rpm or 250 rpm followed by resuspension in phosphate-buffered saline and opsonization in a suspension of DMEM with non-heat inactivated fetal bovine serum and normal mouse serum (Jackson ImmunoResearch, 015-000-120) for 20 minutes.
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bioRxiv - Systems Biology 2023Quote: The following secondary antibodies were used for staining: Cy™3 AffiniPure Fab Fragment Goat Anti-Mouse IgG2a (Jackson ImmunoResearch, Cat# 115-167-186), Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for 1 hour at room temperature with goat anti-mouse IgG horseradish peroxidase (HRP) (Jackson ImmunoResearch, 115-035-166): IgG1 and IgG2a/b levels were quantified using goat anti-mouse IgG1 HRP (Southern Biotechnology Associates ...
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bioRxiv - Pathology 2023Quote: Slides were then washed and incubated for 60 minutes at room temperature with the secondary antibody: 1:100 dilution of DyLight 594 conjugated mouse anti-FITC (Jackson Immunoresearch 200-512-037).
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed 3 × 5 min with TBS-T and incubated with IRDye goat-anti-mouse or goat-anti-rabbit secondary antibodies (1:10,000 Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following secondary antibodies at the indicated dilutions: Cy3-conjugated goat anti-mouse (Jackson ImmunoResearch 115-165-003, 1:1000), Cy3-conjugated goat anti-rat (Jackson ImmunoResearch 112-165-003 ...
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bioRxiv - Genetics 2022Quote: ... we dissected larval brains and stained the samples with the primary antibody NC82 (Developmental Studies Hybridoma Bank, 1:100) and the secondary antibody Cy5 donkey anti-mouse antibody (Jackson ImmunoResearch Laboratories; 1:400) to visualize neuropiles ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were used: Alexa fluor 488 anti-mouse antibody (Life A21241) and Cy3 anti-rat antibody (Jackson Immunoresearch 712-166-153). Fibers were visualized and imaged by Carl Zeiss Axio Imager Apotome using 40X Plan Apo 1.4 NA oil immersion objective ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated with the secondary antibody (goat-anti-mouse, FITC conjugated, 1:200 in PBSA, Jackson ImmunoResearch Europe Ltd, #115-095-146) for 1 hour at room temperature in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... batches of 5.0 × 106 fluorescent streptavidinylated DAAM particles at a time were incubated with 500 nM biotinylated mouse immunoglobulin G (IgG, Jackson Immunoresearch 015-000-003). After all coatings ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary anti-neurofilament antibody (DHSB, 2H3) was used at a 1:20 dilution and anti-mouse HRP-conjugated secondary antibody (Jackson ImmunoResearch, 115-035-003) was used at 1:1000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... A10040),donkey anti-mouse IgG Alexa Fluor 555 (Invitrogen, A31570, donkey anti-rabbit IgG Alexa Fluor 647 (Invitrogen, A31573, donkey anti-mouse Cy5 (Jackson ImmunoResearch, 715-175-150), donkey anti-rat Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated in α-rabbit or α-mouse IgG conjugated to TRITC (Jackson ImmunoResearch, #711-025-152 and #715-025-150, 1:300) diluted in 5% sheep serum in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... then for one hour with a horse-radish peroxidase-conjugated goat anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with 6 nm Colloidal Gold AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 115-195-146, 1:20, AB_2338728) for 30 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Yeast were washed with PBS-BSA and labeled with secondary Myc-FITC antibody and APC-conjugated goat anti-mouse-IgG (Jackson ImmunoResearch 115-605-008). As with ACE2-binding titrations ...
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bioRxiv - Cell Biology 2023Quote: ... the blot was washed 3 times with 1× TBST for 15 min each at RT and then incubated with horseradish peroxidase–conjugated goat anti-mouse IgG antibody (1:20000, Jackson ImmunoResearch 115-035-003) diluted in 5% non-fat milk for 1 h at RT ...
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bioRxiv - Genomics 2024Quote: ... coverslips were washed and RL1 antibody was immunostained with goat-anti-mouse-Alexa-Fluor-488 secondary antibody (Jackson ImmunoResearch Laboratories, cat#115-545-003) diluted 1:500 in PBS/NGS ...
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bioRxiv - Cancer Biology 2024Quote: ... AffiniPure goat anti-mouse IgG (H+L) (0.4 µg/mL in PBS supplemented with 1% w/v BSA; Jackson ImmunoResearch, West Grove, PA, USA) was added ...
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bioRxiv - Systems Biology 2024Quote: ... Secondary antibodies used in this study were: HRP (horseradish peroxidase)-conjugated Goat-anti-mouse IgG secondary antibody (1:10000; Jackson ImmunoResearch # 115-035-003) and Alexa Fluor™ Plus 647 conjugated goat anti-rabbit IgG secondary antibody (1:3000 ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Cell Biology 2020Quote: ... cells immunostained for E-cadherin with Alexa Fluor 488 secondary antibody were blocked with 26 µg/ml of AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Inc, West Grove, PA) in 5% NGS in PBS overnight before being stained for LAMP1 with Alexa Fluor 594-conjugated goat anti-mouse secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were washed with PBS and immunostained in PBG with secondary antibodies conjugated to fluorescein isothiocyanate (FITC) (donkey anti-mouse IgG; Jackson ImmunoResearch Lab, Inc., 1:125) or Cy3 (donkey anti-rabbit ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed and incubated at 37°C for 60 min with the appropriate secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase-conjugated anti-mouse or anti-rabbit (115-035-174 and 211-032-171, Jackson ImmunoResearch Laboratories; both 1:10,000).
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bioRxiv - Physiology 2020Quote: ... The secondary antibodies were peroxidase-conjugated AnffiniPure Goat Anti-Rabbit or mouse IgG H&L (Alexa Fluor® 488; Jackson ImmunoResearch Laboratories, West Grove, PA) at 1:500 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... antibody and detected with 1:200 anti-rabbit CY3 (Jackson Immunoreserach, cat# 715-165-151) or 1:200 Alexa 488 Mouse (1:200, Jackson Immunoresearch, cat# 715-545-150) secondary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated 1 h at room temperature with goat anti-rabbit (1:10000) or anti-mouse (1:5000) HRP-conjugated secondary antibodies (Jackson ImmunoResearch Europe Ltd, Newmarket, UK) and signal was detected with WesternBright ECL Spray (Advansta ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then incubated at 37°C for 60 min with a secondary antibody (Alexa Fluor 594 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG, 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer before final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using the following secondary peroxidase conjugated antibodies: goat anti-rabbit and anti-mouse IgG-HRP (111-035-003, 115-035-003; Jackson ImmunoResearch Laboratories, Inc. Cambridge, UK) and enhanced chemiluminescent (ECL ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and then incubated for 2 h at room temperature with Alexa 488 goat anti mouse 1:1000 (Jackson ImmunoResearch, cat n° 115-545-166), Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Neuroscience 2022Quote: ... The sections were then processed for double immunofluorescence by using in combination anti-Homer1bc and anti-PSD-95 primary antibodies and the appropriate fluorescent secondary antibodies (anti-mouse or anti-rabbit 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) following the same protocol used for mouse brain tissue (see Pizzo et al. ...