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Citations for Jackson ImmunoResearch :
101 - 150 of 216 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times for 10 minutes in TBST and incubated in horseradish peroxidase-conjugated secondary antibodies (1:10,000; Jackson ImmunoResearch) at room temperature in TBST with 5% milk for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS (3 times) and stained with Cy3-conjugated donkey anti-rat IgG (Jackson ImmunoResearch Laboratories; 1.25 µg/ml). To detect total channels ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed in TBST (TBS with 0.05% Tween 20) for 30 min (3 × 10 min) and incubated with secondary antibodies conjugated to horseradish peroxidase (Jackson ImmunoResearch) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3×15 min with PBS and incubated with a donkey anti-rabbit biotinylated secondary antibody (Jackson ImmunoResearch) at 1:500 in blocking buffer (2-hours ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in 1 × TBS (3 × 10 min) before being incubated in a secondary antibody (1:10,000 HRP-conjugated goat anti-rabbit, Jackson ImmunoResearch), in blocking solution described above ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 3-5 h with a secondary antibody (Alexa Fluor® 488 anti rabbit; Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch, Cambridge ...
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bioRxiv - Physiology 2021Quote: ... After 3 washes and a 1-hour incubation with the secondary anti-goat antibody (3.3 ng/μL, 705605003; Jackson ImmunoResearch) and DAPI (1 μg/μL ...
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bioRxiv - Neuroscience 2021Quote: ... brains were left rocking at 4°C for 1-3 nights in 0.1% PBS-T supplemented with blocking agent normal goat serum (Jackson ImmunoResearch) at a 1:20 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Kidney were incubated in blocking buffer consisting of PTxwH with 3% normal donkey serum (Jackson Immunoresearch, West Grove, PA, USA) at 37°C for 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 × 10 min with PBST (1X PBS with 0.5% Triton X-100) and incubated in appropriate secondary antibodies (Jackson ImmunoResearch) conjugated to Alexa 488 ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were permeabilized with 0.3% Triton X-100 in PBS (PBS-T) and blocked with 3% normal donkey serum (Jackson ImmunoResearch) in PBS-T (PBS-T DS ...
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bioRxiv - Neuroscience 2023Quote: ... recorded slices were blocked in PBS containing 0.3% Triton (PBS-T 0.3%) and 3% normal donkey serum (NDS; Jackson ImmunoResearch, USA) at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: Sections were rinsed 3 x 15 min with PBS and incubated in appropriate secondary antibodies (Jackson ImmunoResearch, West Grove, PA) conjugated to Alexa 488 ...
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bioRxiv - Neuroscience 2024Quote: ... The floating sections were washed 3×10 min with 1x PBS and blocked with 5% normal donkey serum (Jackson ImmunoResearch), 2% fish gelatin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in a blocking solution composed of PBST with 3% normal donkey serum (Jackson ImmunoResearch, #017-000-121) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed for 15 min with 1x PBS/0.1% Tween20 post primary antibody treatment followed by 1 hour incubation at room temperature with 1x PBS+3%BSA containing secondary antibodies anti rabbit IgG-Alexa 488 or anti rat IgG-Alexa 594 (all from Jackson ImmunoResearch). The nuclear stain DAPI (2μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was incubated for 3 hrs at room temp with biotinylated donkey anti-rabbit (1:500, Jackson Immunoresearch 711-065-152), then washed ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rinsed three times in 1X PBS and incubated in secondary antibody solution Cy-3 donkey anti-rabbit (1:500; Jackson ImmunoResearch) or Cy-3 donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated for 3-h at room RT in biotin-streptavidin conjugated AffiniPure goat anti-rabbit IgG (1:200; Jackson Immunoresearch), washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed with 0.01M PBS (10 Min × 3 times) and incubated in FITC or texas red-conjugated secondary antibody (1:200, Jackson ImmunoResearch Laboratories) in dilution buffer (1% BSA and 0.3% Triton X-100 in 0.01M PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... brain slices were incubated with the following secondary antibodies in the blocking solution at RT 3 hours: donkey anti-mouse-AlexaFluor 488 (715-545-150; 1:300; Jackson ImmunoResearch), donkey anti-G.P.-RRX (706-295-148 ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated for 3 h at room temperature with 1:250 anti-mouse DyLight 650 (Jackson ImmunoResearch, Burlington, ON, Canada) secondary antibody diluted in 0.1 M PB containing 1.5% heat inactivated horse serum ...
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bioRxiv - Cell Biology 2022Quote: ... 2016)) and secondary antibodies were anti-rat coupled to Alexa Fluor 488 and anti-rabbit coupled to Cyanine 3 (Jackson ImmunoResearch) both diluted to 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were then incubated for 60 min at 37°C with the respective secondary antibodies diluted in 3% BSA: AMCA-conjugated anti-chicken (1:200; Jackson ImmunoResearch), Alexa Fluor® 488 conjugated anti-rabbit (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed 3 times for 5 min in PBS and incubated for 30 min with a cyanine 3-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch) and DAPI (4′,6-diamidino-2-phenylindole).
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bioRxiv - Neuroscience 2020Quote: ... the brains were washed three times at room temperature in 0.25% PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch 115-165-062 ...
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bioRxiv - Neuroscience 2020Quote: ... they were washed 3 times with 1X PBS and then incubated with 1:1000 secondary antibodies (Donkey-anti-Rat Cy3, Jackson ImmunoResearch 712-165-153 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 3 hr in secondary antibody (anti-Chicken AlexaFluor 488 and anti-Rabbit Cy3, each 1:250, Jackson ImmunoResearch) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed with PBS 3 times and incubated with a secondary antibody (Alexa 488 anti-chicken, 703-545-155, Jackson ImmunoResearch) for 1 h at RT and then washed with PBS 3 times for 10 m ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with PBS containing 3% normal donkey serum and 0.3% Triton X-100 and then incubated with 100 μg/ml unconjugated anti-human IgG Fab fragments (H+L, Jackson Immunoresearch). Slides were then incubated with FM or HC serum diluted 1:500 in PBS with 1% normal donkey serum and 0.1% Triton X-100 ...
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mTORC1-dependent SOCE activity regulates synaptic gene expression and muscle response to denervationbioRxiv - Physiology 2024Quote: ... sections were fixed with PFA 4% and then blocked with 3% IgG free BSA and AffiniPure Mouse IgG Fab Fragments (Jackson ImmunoResearch) in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: Sections were washed 3 times and incubated with species appropriate and minimally cross-reactive fluorophore-conjugated secondary antibodies (1:500, Jackson ImmunoResearch) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... and blocked in 3% milk– PBS-T prior to incubation with serial dilutions of serum or the mouse IgG standard (Invitrogen, Jackson ImmunoResearch) in 1% milk-PBS-T for 2 hrs at RT ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then washed 3 times for 10 min with PBS and stained with the suitable secondary antibody (1:200, Jackson ImmunoResearch) and DAPI (4,6-diamidino-2-phenylindole dihydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated for 3 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (1:1,000; Thermo Fisher Scientific or Jackson ImmunoResearch). Whole retinas were flattened with photoreceptor side down onto nitrocellulose filters ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... followed by a 3-h incubation with polyclonal Donkey anti-Goat Alexa flour 488 antibody (AB 2340428, Jackson immunoresearch, PA, USA). These antibodies were diluted by ×500 ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Neuroscience 2023Quote: ... one slice for each animal for each treatment condition was incubated for 24h at room temperature on shaking incubator (Titer Plate Shaker, Lab-Line Instruments) with 3% donkey serum (Jackson Immunoresearch), 1% Triton-X (ACROS Organics) ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated for 3 hours at room temperature with Cy3-conjugated anti-sheep secondary antibody (Jackson ImmunoResearch, 715-165-147) diluted 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated for 3 hours at room temperature with Cy5-conjugated anti-sheep secondary antibody (Jackson ImmunoResearch, 713-175-147) diluted 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS and immediately transferred to the secondary antibody solution: Cy3 goat anti-mouse (1:500, Jackson ImmunoResearch) for 2 hours at home temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times (15 minutes) in TBS and incubated with a donkey antirabbit biotinylated secondary antibody (Jackson ImmunoResearch Laboratories) at 1:400 in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... IgG isotype antibodies binding to the diverse antigens were detected with Cy-3 conjugated anti-human IgG (1:1000) from Jackson ImmunoResearch Laboratory ...
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bioRxiv - Physiology 2024Quote: ... blocked with 3 % BSA for 1 hour and incubated with AffiniPure Fab Fragment Goat Anti-Mouse IgG (H + L) (Jackson ImmunoResearch) in PBS (20 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating brain sections were blocked in 3% normal donkey serum for 2 h (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then incubated with rabbit anti-DsRed primary antibody (1:1,000 ...