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Citations for Jackson ImmunoResearch :
101 - 150 of 4221 citations for 2E 4E 2 4 Octadien 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... RBD antigen binding during selection Round 4 was evaluated using a 1:1000 dilution of Streptavidin-Alexa Fluor 647 secondary (Jackson ImmunoResearch). Use of a different secondary reagent selected for true RBD binders and de-enriched the population for anti-His-tag secondary reagent binders (data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the primary antibodies overnight at 4°C and then the proper secondary antibodies (1:500, Alexa Fluor 488, 594, 647 conjugated; Jackson ImmunoResearch) for 2 h at room temperature in the same blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... All sections were rinsed three times for 15 min in PBS-T and incubated for 4 hours at ∼20 °C in secondary antibodies in the blocking solution (Cy3 donkey anti-guinea pig, 1:400, Jackson ImmunoResearch, catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... overnight at 4°C, and with fluorescence (Alexa Fluor 488, 568, 647)-conjugated secondaries (Jackson ImmunoResearch Laboratories or Abcam; 1:1000) overnight at 4°C at indicated dilutions ...
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bioRxiv - Cell Biology 2023Quote: ... 1:5) at 4 °C overnight and with the secondary FITC-conjugated or TRITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were incubated in the dark at 4°C for 24 h with secondary antibodies (1:500, Alexa Fluors 488, 555, 594 and 647, Jackson Immunoresearch). For CASK immunostaining experiments larvae were incubated in Hoechst stain (1:8000 ...
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bioRxiv - Neuroscience 2024Quote: Cells were fixed with 4% paraformaldehyde (PFA) and blocked for 1 hour in phosphate-buffered saline (PBS) supplemented with 10% normal donkey serum (Jackson ImmunoResearch) and 0.1% Triton X-100 ...
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bioRxiv - Genomics 2024Quote: ... and then incubated with primary antibodies in A+t buffer at 4°C overnight and then with fluorescently-labeled secondary antibodies (1:200 dilution, Jackson ImmunoResearch). Testes were stained with 0.5 μg/mL DAPI/PBS and mounted in 80% glycerol on slides ...
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bioRxiv - Developmental Biology 2021Quote: ... MAB8887) and for 2 hours at room temperature with Cy3- or AF488-conjugated secondary antibodies (1:500, Jackson Immunoresearch). For amplified staining ...
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bioRxiv - Developmental Biology 2021Quote: ... and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500, Jackson Immunoresearch, West Grove, PA). For staining on paraffin sections antigen retrieval was performed by either 1mg/ml trypsin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy3-labeled species-specific secondary antibodies for 2 h at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch). To avoid cross talk between multiple fluorophores ...
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bioRxiv - Cell Biology 2022Quote: ... CyTM3 AffiniPure F (ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) (1: 200, Jackson ImmunoResearch, Cat No: 711166152) and DyLight 488 Horse Anti-Mouse IgG Antibody (1 ...
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bioRxiv - Microbiology 2024Quote: ... followed by 30 min incubation with 1:3000 human secondary F(ab′)2 Goat Anti-Human IgG (Jackson ImmunoResearch) and fluorescence measurement on a Bio-Plex 2000 system ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Iba1 (1:1000) in PBS with 0.3% Triton X-100 (MilliporeSigma) and 2% normal donkey serum (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... 2–3 hours in secondary antibody 1:500 anti-chicken-IgY Alexa488-conjugated (Jackson ImmunoResearch cat# 703-545-155) in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Extended Data Table 1) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times in PBS and incubated for 60–90 min at room temperature or overnight at 4°C with the Alexa Fluor 488- and 594 and 647-conjugated secondary antibodies (1:500; Thermo Fisher Science or Jackson ImmunoResearch).
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibody overnight at 4°C and secondary antibody (anti-rabbit-Alexa Fluor 647, 1:400, Jackson ImmunoResearch, Inc., West Grove, PA) for 3-4 hours in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500, Jackson ImmunoResearch #706606148). Tissues were rinsed and mounted using Prolong diamond antifade (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... slices were incubated at 4°C for 48–72 h in pSTAT3 antibodies (1:3000) in 3% normal donkey serum (Jackson ImmunoResearch Labs) with PBT and 0.02% sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated ON at 4°C followed by 3 washes with PBS and the corresponding secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) containing 4′,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Biophysics 2024Quote: ... Nonspecific cross-reactivity was blocked by incubating the samples for 4 h in 1% BSA/PBS (Jackson ImmunoResearch, West Grove, PA, USA). The cells were then incubated with primary antibodies anti-RyR (IgG1 ...
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bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... F(ab’)2 (Jackson ImmunoResearch) and PE-conjugated streptavidin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with anti-rabbit-Cy2 and anti-rat-Cy3 secondary antibodies for 2 h (1:400, Jackson Immunoresearch, #711-225-152 ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibody against RFP was detected with a 2-hour incubation in diluted donkey-anti-rabbit-IgG-Cy3 antibody in blocking buffer (1:1,000, Jackson ImmunoResearch). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation (2 h) with appropriate fluorescent secondary antibodies (Cy3-or Cy2-coupled fluorescent secondary antibodies, 1:200; Jackson ImmunoResearch Laboratories catalog #715-165-150 and #715-545-150 respectively) ...
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bioRxiv - Cell Biology 2023Quote: ... or allophycocyanin-conjugated (APC) AffiniPure F (ab’)2 fragment goat anti-Rat IgM (1:100, Jackson ImmunoResearch, 112-136-075) at 4°C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... MilliporeSigma) was applied for 2 h at RT followed by secondary antibody (fluorophore-conjugated IgG, 1:500; Jackson ImmunoResearch Laboratories) for 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... and 1:1000 dilution of goat anti-human Fc F(‘ab)2 Alexa Fluor 488 (Jackson ImmunoResearch, 109-546-170) on ice for 4 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed and incubated for 2 h with FITC-conjugated donkey anti-rabbit antibody (1:150; RRID:AB_2315776, Jackson ImmunoResearch, US) in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was again rinsed three times in 0.1M NaPb for 10 min followed by 2-h in biotin-SP-conjugated donkey anti-rabbit (1:300; Jackson ImmunoResearch) secondary antibody and AlexaFluor-488 donkey anti-chicken secondary antibody (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-mouse conjugated with HRP (1:5,000, 2 hours at room temperature, catalog no. 111-035-146; Jackson ImmunoResearch), goat anti-rat conjugated with HRP (1:5,000 ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-rat conjugated with HRP (1:5,000, 2 hours at room temperature, catalog no. 115-035-143; Jackson ImmunoResearch). Western blots were developed with Thermo Scientific SuperSignal West Pico and Femto PLUS Chemiluminescent Substrate ...
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bioRxiv - Neuroscience 2021Quote: ... animals were incubated overnight at 4°C in Alexa Fluor 488 donkey anti-chicken secondary antibody (1:1000, Jackson ImmunoResearch, 703-545-155), Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch, Westgrove, PA; 1:10,000) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2% Triton X-100 and incubated overnight at 4°C with a CY5-conjugated secondary antibody (1:400, goat anti-mouse, Jackson Immunoresearch, Suffolk, UK), washed in 1x PBS and embedded in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by further rinses in 0.01 M PBS (3 × 10min) and a 12h incubation at 4 °C in biotin-streptavidin conjugated AffiniPure IgG (anti-rabbit-streptavidin Jackson Immunoresearch, 1:200), followed by three washes with 0.01 M PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Supplementary Table 4) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cells were stimulated with a suboptimal concentration of pre-mixed complexes composed of 0.056 µg mL-1 biotinylated F(ab’)2 goat anti-mouse IgM (µ-chain specific) (Jackson ImmunoResearch), ∼3 µg mL-1 C3dg-biotin (produced in house ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with secondary antibodies R-Phycoerythrin AffiniPure F (ab’) 2 Fragment Donkey Anti-Rat IgG (1:300; 712-116-153; Jackson ImmunoResearch) or Alexa Fluor 568 donkey anti-rabbit IgG (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in a secondary antibody solution at room temperature for 2 h with protection from light (1:500, Alexa Fluor 647 donkey anti-chicken, Jackson Immunoresearch, USA ...
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bioRxiv - Neuroscience 2021Quote: ... tissue is washed 10 times in 1X PBS for 1 min each and then incubated for 2 hours in blocking buffer + secondary antibodies from Jackson ImmunoResearch Laboratories (donkey anti-rabbit 488 ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated for 1 hour at room temperature with HRP conjugated-(Fab)2 Goat antimouse IgG (Fc specific) or Goat anti-mouse IgM (both 1/10000, Jackson Immunoresearch). Then ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed 3 × 5 minutes in PBS prior to a 2 hour incubation with a biotin-conjugated donkey anti-rat secondary antibody (1:500, 712-065-153, Jackson ImmunoResearch). The primary–secondary antibody complexes were detected via peroxidase-driven precipitation of diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2020Quote: ... Several rinsing with TBS was followed by incubation in secondary antibody solution for 2 hours (1:500 Cy3 conjugated goat anti-rabbit IgG, #134845, Jackson ImmunoResearch).
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% 1X TBS-T followed by 2 hours incubation at room temperature with secondary anti-mouse or anti-rabbit antibody (1:5000, Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... B cells were plated at a concentration of 5L×L105 cells per 200LμL in round-bottom 96-well plates treated with 0.1% B16F10 melanoma cell lysis or 10 µg/mL goat anti-mouse IgM F(ab’)2 (115-006-020, Jackson ImmunoResearch). B cells were treated with NR-V04 or DMSO for 24 hours and collected for flow cytometry analysis.
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bioRxiv - Immunology 2023Quote: ... Indo bound/Indo free ratio baseline was recorded for 1 min before addition of pre-warmed anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch) to a final concentration of 2 or 10 μg/ml ...