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Citations for Jackson ImmunoResearch :
101 - 150 of 572 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... larvae were incubated in the dark at 4°C for 24 h with secondary antibodies (1:500, Alexa Fluors 488, 555, 594 and 647, Jackson Immunoresearch). For CASK immunostaining experiments larvae were incubated in Hoechst stain (1:8000 ...
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bioRxiv - Plant Biology 2024Quote: ... and the roots were incubated overnight at 4°C with a mixture of secondary anti-rabbit conjugated with CY3 and anti-rat conjugated with CY2 (Jackson ImmunoResearch) each diluted to final concentration 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... for overnight at 4 °C and incubated with secondary antibodies like donkey anti-Rabbit IgG-HRP (Jackson ImmunoResearch, 711-035-152), donkey anti-mouse IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: Cells were fixed with 4% paraformaldehyde (PFA) and blocked for 1 hour in phosphate-buffered saline (PBS) supplemented with 10% normal donkey serum (Jackson ImmunoResearch) and 0.1% Triton X-100 ...
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bioRxiv - Genomics 2024Quote: ... and then incubated with primary antibodies in A+t buffer at 4°C overnight and then with fluorescently-labeled secondary antibodies (1:200 dilution, Jackson ImmunoResearch). Testes were stained with 0.5 μg/mL DAPI/PBS and mounted in 80% glycerol on slides ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with the following secondary antibodies at 10 μg/mL at room temperature for 4 hours: FITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Extended Data Table 1) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times in PBS and incubated for 60–90 min at room temperature or overnight at 4°C with the Alexa Fluor 488- and 594 and 647-conjugated secondary antibodies (1:500; Thermo Fisher Science or Jackson ImmunoResearch).
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibody overnight at 4°C and secondary antibody (anti-rabbit-Alexa Fluor 647, 1:400, Jackson ImmunoResearch, Inc., West Grove, PA) for 3-4 hours in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500, Jackson ImmunoResearch #706606148). Tissues were rinsed and mounted using Prolong diamond antifade (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... treated with 1M HCl for 1 hour and incubated overnight at 4°C with anti-BrdU antibody following incubation with Alexa Fluor-488 conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). Slides were mounted in mounting medium for fluorescence with DAPI (Vector Laboratories) ...
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bioRxiv - Physiology 2024Quote: ... slices were incubated at 4°C for 48–72 h in pSTAT3 antibodies (1:3000) in 3% normal donkey serum (Jackson ImmunoResearch Labs) with PBT and 0.02% sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated ON at 4°C followed by 3 washes with PBS and the corresponding secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) containing 4′,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Biophysics 2024Quote: ... Nonspecific cross-reactivity was blocked by incubating the samples for 4 h in 1% BSA/PBS (Jackson ImmunoResearch, West Grove, PA, USA). The cells were then incubated with primary antibodies anti-RyR (IgG1 ...
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bioRxiv - Neuroscience 2021Quote: ... animals were incubated overnight at 4°C in Alexa Fluor 488 donkey anti-chicken secondary antibody (1:1000, Jackson ImmunoResearch, 703-545-155), Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch, Westgrove, PA; 1:10,000) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2% Triton X-100 and incubated overnight at 4°C with a CY5-conjugated secondary antibody (1:400, goat anti-mouse, Jackson Immunoresearch, Suffolk, UK), washed in 1x PBS and embedded in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (4°C overinight) and then secondary peroxidase-conjugated Goat Anti-Rabbit IgG (Jackson ImmunoResearch 111-035-144) or peroxidase-conjugated Goat Anti-Mouse IgG (Jackson Immuno Research 115-035-174 ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by further rinses in 0.01 M PBS (3 × 10min) and a 12h incubation at 4 °C in biotin-streptavidin conjugated AffiniPure IgG (anti-rabbit-streptavidin Jackson Immunoresearch, 1:200), followed by three washes with 0.01 M PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg/ml) and donkey (711-166-152, 711-546-152, 715-166-150 or 715-546-151, Jackson ImmunoResearch, 1.5 µg/ml) secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
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bioRxiv - Systems Biology 2021Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... and the following secondary antibodies at 4°C for 24 h: Alexa Fluor 488 donkey anti-chicken (1:250, Jackson ImmunoResearch, #703-545-155) and Alexa Fluor 568 donkey anti-rabbit (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 4-6 h in the following secondary antibodies: donkey anti-rat 488 (1: 500; Jackson Immunoresearch, 712-546-153), donkey anti-rabbit-Cy3 (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with 1 μM biotinylated peptides for 1h before they were fixed with 4% PFA and incubated with Streptavidin-AlexaFluor 594 antibody (Jackson ImmunoResearch, West Grove, PA) and Hoechst 33342 (Novus Biologicals ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature [6-8,10] ...
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bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed 3X 15 min in 0.1M PBS and then incubated at 4°C for 24 h with donkey anti-chicken-488 (1:1000; 703-545-155, Jackson Immunoresearch, West Grove, PA) and donkey anti-rabbit-CY3 (1:400 ...
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bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti-guinea pig Alexa Fluor (AF) 488 (Catalog #:706-545-148) had a 1:500 dilution and were stored overnight at 4 °C (Jackson ImmunoResearch; West Grove, PA, USA). Sections were then mounted and cover-slipped with using 50% glycerol in 0.02M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed three times for 20 min each in PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch, AB_2338680, 1:200 v/v) and goat anti-rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... Cells were then washed and incubated with primary antibodies diluted in blocking solution overnight at 4 °C followed by incubation with an AlexaFluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch, 711-545-152, 1:500) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-prepared immunopanning dishes were setup as follows: a 10cm petri dish was incubated at 4°C overnight with 30ul anti-rat antibody (Jackson ImmunoResearch Laboratories, Inc, 112-005-167) in 10 ml 50 mM Tris-HCL (pH 9.5) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and immunofluorescence was performed as described previously [54] using appropriate primary and secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were washed in PBST four times for 4 hours and incubated overnight with biotin goat anti-mouse IgG (Jackson ImmunoResearch ref. 115-066-071; 1:500) and then with streptavidin-Cy3 (Jackson ImmunoResearch ref ...