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Citations for Jackson ImmunoResearch :
1051 - 1100 of 4192 citations for PD 1 Human 147a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: we used Roche’s mixture of two mouse mAbs (1/5000) as primary antibodies and HRP-conjugated AffiniPure goat anti-mouse IgG (H+L) secondary antibodies (Jackson Immunoresearch, 1/5000). In all cases reacting bands were detected with Clarity western ECL substrate (Biorad Laboratories).
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... using a rabbit anti-Glra4 antibody (1:100; Chemicon Cat# AB9696) with a goat anti-rabbit Cy3 secondary (1:200, Jackson ImmunoResearch Cat# 705165003). We examined the pattern of GlyRα4 relative to the bands formed by the ON and OFF SAC dendrites in the IPL ...
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bioRxiv - Microbiology 2021Quote: ... Infected foci were visualised using mouse monoclonal Anti-Arenavirus (OW) rGPC, Clone KL-AV-1B3 (BEI Resources, 1:200) followed by anti-mouse AlexaFluor488 secondary (Jackson ImmunoResearch; 1:1000). Virus titres were calculated as focus forming units per mL (FFU/mL ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µL of secondary antibody solution (1:6 pre-absorbed α-rat FITC, diluted to 1:200 in PBS-BSA; 712-095-153, Jackson ImmunoResearch Laboratories) was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µL of secondary antibody solution (1:6 pre-absorbed α-mouse Cy3, diluted to 1:200 in 1X PBS-BSA; 115-165-003, Jackson ImmunoResearch Laboratories) was added to each well ...
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bioRxiv - Bioengineering 2020Quote: ... samples were incubated with secondary antibodies (1:400 donkey anti-mouse Alexa Fluor®647 or 1:100 donkey anti-goat Alexa Fluor®647; Jackson ImmunoResearch) and 1:1000 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2019Quote: ... The slices were incubated for 3 - 4 hr at room temperature with Cyanine-3-conjugated (Cy3) to streptavidin (1:500 or 1:250 Jackson ImmunoResearch labs, Inc) in blocking buffer (PBS with 5% donkey serum and 0.3% Triton X-100) ...
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bioRxiv - Immunology 2022Quote: ... followed by staining with goat-anti-mouse IgG F(ab’)2-HRP (Jackson ImmunoResearch; 1:5000 diluted in TBS-T with 1% BSA). All incubations were 1h at RT and followed by extensive washing with TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were washed and incubated at room temperature for 1 hour with Alexafluor 488-conjugated goat anti-rabbit IgG (#111-545-144, Jackson ImmunoResearch, 1:500). After secondary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the secondary antibodies (goat anti rabbit ALEXA 488, 1:500 dilution, ThermoFisher Scientific; goat anti–rat Cy3, 1:300 dilution, Jackson Immunoresearch Laboratories Inc.) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated 1 hour at room temperature with the following secondary antibodies: Cy3 donkey anti-mouse (#715-165-151, Jackson Immunoresearch, 1:500), Alexa488 donkey anti-rabbit (#715-545-150 ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibodies were incubated for 1 hour at room temperature (goat anti-rabbit 488 Jackson ImmunoResearch Labs, 1:500 in PBS+0.5% Tween). Samples were rinsed with PBS+0.5% Tween 3 × 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... was used in a 1:300 dilution followed by anti-rabbit HRP secondary antibody diluted to 1:2500 (Jackson ImmunoResearch 111-035-003). For the loading control ...
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bioRxiv - Neuroscience 2024Quote: ... 1:500, #711-065-152; Jackson ImmunoResearch Labs, West Grove, PA, USA or for goat, 1:500, #711-065-147; Jackson ImmunoResearch Labs), Cy3 (for guinea pig ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 1:500 mouse anti-Myc (CST #2276) and 1:3000 goat anti-mouse HRP secondary antibody (Jackson ImmunoResearch #115-035-003). For the loading control ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488-conjugated goat-anti-rabbit (1:500) and Alexa Fluor 647-conjuated goat-anti-mouse (1:800) secondary antibodies (Jackson Immunoresearch; West Grove, PA) were diluted in a solution consisting of 2% goat serum ...
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bioRxiv - Developmental Biology 2019Quote: ... Burlington, NC) or mouse α-actin (C4, 1:40,000, MilliporeSigma) followed by donkey α-mouse horseradish peroxidase (1:10,000, Jackson ImmunoResearch, West Grove, PA). Immunoblots were developed using SuperSignal™West Pico/Femto Sensitivity substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in species-appropriate secondary antibodies (Cy3-conjugated donkey anti-rabbit, 1:300, cat# 711-165-152, and Alexa488-conjugated donkey anti-chicken, 1:100, cat# 703-545-155, Jackson ImmunoResearch, West Grove, CA) for 1h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202; anti-rabbit-Alexa568, Invitrogen A10042; anti-sheep-Cy3, Jackson ImmunoResearch 713-165-147). Coverslips were mounted in FluoromountG (SouthernBiotech ...
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bioRxiv - Biophysics 2021Quote: ... Transverse 50 μm sections were obtained using Leica microtome and stained using mouse monoclonal β-tubulin antibody (E7, DSHB; 1-2 μg/ml) followed by secondary goat anti-mouse Cy3 antibody (Jackson ImmunoResearch; 1:300). To visualize actin filaments in embryos microinjected with 0.5 μm nanoparticles ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The membrane was washed three times with TBST for 10 min and incubated for 1 hour with 1:10,000 diluted peroxidase-conjugated anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA, USA) in 5% skim milk in TBST ...
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bioRxiv - Genetics 2020Quote: ... membranes were incubated for 1 hr at room temperature with species-specific horseradish peroxidase conjugated secondary antibodies (1:10000, Jackson ImmunoResearch, West Grove, PA) diluted in 2% non-fat milk in TBST ...
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bioRxiv - Microbiology 2020Quote: ... Beads were then washed and incubated with anti-rabbit FITC (1:100, KPL, 02-15-16) or anti-mouse APC (1:50, Jackson ImmunoResearch, 115-135-164) secondary antibodies for 1 hour at room temperature with gentle shaking ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed for three times with 1× PBS for 5 min at room temperature and incubated for 1 h at room temperate with appropriate Cy3 secondary antibodies (dilution 1:200, Jackson ImmunoResearch, West Grove, PA) and 0.1% saponin ...
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bioRxiv - Genetics 2020Quote: ... Slides were washed in PBS + 0.05% Tween and incubated with Cy3- or Alexa 488-conjugated secondary antibodies (Jackson Immunoresearch, 1:700; Invitrogen, 1:1000) for 1.5 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3x with PBS and incubated for 1 h at RT in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were 1:200 goat anti-guinea pig Alexa Fluro 488 (1:200 for Fig. 2B; 106-545-003, Jackson ImmunoResearch, West Grove, PA), goat anti-guinea pig Cy3 (1:25 for Fig ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were goat anti-guinea pig IgG (1: 200, Alexa Fluor 488 conjugated, Jackson ImmunoResearch, cat # 106545003; 1:200, Alexa Fluor 647 conjugated, Jackson ImmunoResearch, cat # 106605003).
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3 times for 10 mins with TBS-Tween buffer and incubated for 1 h at RT with anti-rabbit (HRP) secondary antibody (1:20000, Ca# 711-035-152; Jackson ImmunoResearch Lab, Pennsylvania, USA) for chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were rinsed three times in 1X wash buffer for 2m at RT before adding secondary antibody (goat anti-chicken Alexa 647, 1:250 in PBS with 1% BSA; Jackson Immunoresearch #103-605-155). Slides were rinsed five times in 1X wash buffer for 2m at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were subsequently washed with saponin buffer or FACS buffer and incubated for 1 hr with secondary antibodies: goat anti-rabbit Alexa 488 (1:500, Jackson Immunoresearch, 111-545-144) or goat anti-mouse IgM-conjugated Alexa 488 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-Mon1a and Fcho2 were used at a concentration of 1:500 followed by peroxidase conjugated goat anti-rabbit IgG (1:10,000) (Jackson ImmunoResearch Labs, West Grove, PA). The blots were developed using Western Lightning reagent (PerkinElmer Life Sciences ...
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bioRxiv - Bioengineering 2023Quote: ... anti-ACK2 primary antibodies were incubated for 2 h in biotinylated secondary antiserum (anti-rabbit 1:2500 or anti-rat, 1:1000; Jackson ImmunoResearch, West Grove, PA) specific to the species of the primary antibodies and were constituted in PBS with 0.32% TX ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Neuroscience 2023Quote: ... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Genetics 2022Quote: ... we dissected larval brains and stained the samples with the primary antibody NC82 (Developmental Studies Hybridoma Bank, 1:100) and the secondary antibody Cy5 donkey anti-mouse antibody (Jackson ImmunoResearch Laboratories; 1:400) to visualize neuropiles ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then washed and stained for 1 hour at room temperature with the following secondary antibodies: anti-hamster Cy3 (Jackson ImmunoResearch 127165160, 1:500), anti-rabbit AlexFluor 488 (Invitrogen A11008 ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then placed in secondary antibodies, Alpha-Bungarotoxin AF555 1:500 (ThermoFischer, #B35451) and Donkey anti-Rabbit AF488 1:450 (Jackson ImmunoResearch, #711-545-152), diluted in PBS1X for 2.5 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... light chain specific (Jackson ImmunoResearch, 211-032-171, 1:5,000). Inputs represent 2-5% of the lysate subjected to immunoprecipitation.
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bioRxiv - Cancer Biology 2021Quote: ... light chain specific (Jackson ImmunoResearch, 115-035-174, 1:5,000), peroxidase IgG fraction monoclonal mouse anti-rabbit IgG ...
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bioRxiv - Cancer Biology 2021Quote: ... they were incubated in secondary antibody (1:100, Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Cy3 AffiniPure Donkey Anti-Guinea pig (1:500, Jackson Immunoresearch), Cy5 AffiniPure Donkey anti-Goat (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and 488 AffiniPure Donkey anti-Mouse (1:500, Jackson Immunoresearch).
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bioRxiv - Neuroscience 2020Quote: ... and biotinylated anti-goat secondary antibody (1:200; Jackson Immunoresearch) both for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... To mark motorneurons goat anti-HRP(1:400, Jackson Immunoresearch) primary antibody staining was performed for overnight at 4°C on the shaker and secondary donkey anti-goat 568(1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescein (FITC)-conjugated donkey anti-rabbit (Jackson ImmunoResearch; 1:500), AF-568 goat anti-mouse (Invitrogen #A11004 ...