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Citations for Jackson ImmunoResearch :
51 - 76 of 76 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... blocked with 0.2 per-cent BSA/PBS for 30 min and successively incubated for 1 h at room temperature with primary and secondary antibodies (1:500; Jackson ImmunoResearch) diluted in PBS contain-ing 0.2 percent BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... Blocking was for 30 min at room temperature with PBST/1%DMSO/2% normal goat serum (Jackson ImmunoResearch, 005-000-121). Primary antibodies were applied overnight at 4 °C while secondary antibodies were applied for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The transfectant cells were incubated with supernatant containing expressed antibodies for 30 minutes then followed by incubation with APC-anti-human IgG (H+L) antibody (Jackson ImmunoResearch, USA). Bound antibodies to the GFP-positive cells were then analyzed by flow cytometry (Attune NxT ...
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bioRxiv - Neuroscience 2024Quote: ... Mg2+ free HBSS and incubated in 12 nm Gold AffiniPure Goat anti-Rabbit secondary antibody (1:30 Jackson ImmunoResearch #111-205-144) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then simultaneously permeabilised and blocked for 30 min at room temperature with a buffer containing 10% donkey serum (Jackson ImmunoResearch, UK) and 0.1% Triton X-100 (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... 4°C) was followed by biotin-conjugated AffiniPure Goat anti-rat (H+L, Jackson ImmunoResearch, West Grove, PA, 30 min, 4°C) followed by Streptavidin-eFluor 450 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated in the secondary antibody mixture: 1:30 diluted 12 nm gold conjugated donkey anti-chicken (Jackson Immunoresearch, 703-205-155), and 1:30 diluted 6 nm gold conjugated donkey anti-rabbit (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2020Quote: ... a fluorescently labelled appropriate species secondary antibodies was used for 30 mins (anti-mouse Alexa 594, anti-rabbit Alexa 488 Jackson ImmunoResearch, 1:300). Cells briefly incubated in Hoechst before being mounted with Ibidi mounting medium.
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bioRxiv - Systems Biology 2021Quote: ... the membranes were incubated for 30 minutes at room temperature with a horseradish peroxidase (HRP)-conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA), which was diluted 1:10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...
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bioRxiv - Bioengineering 2021Quote: ... embryos were washed 3 times (30 min intervals between washes) in the staining buffer and labeled using donkey-anti-rabbit-biotin secondary antibody (Jackson ImmunoResearch, 711-065-152) in the staining buffer at 4°C overnight ...
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bioRxiv - Bioengineering 2021Quote: ... the embryos were washed 3 times in the staining buffer (30 mins intervals between washes) and labeled with Alexa Fluor 488 Streptavidin (Jackson ImmunoResearch, 016-540-084). Labeled embryos were washed 3 times in the staining buffer (30 minutes between washes ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were incubated for 30 minutes in a 1:200 dilution of the goat anti-rabbit secondary antibody conjugated to horse radish peroxidase (Jackson ImmunoResearch #111-036-003). The brown color was produced by reaction with 3,3’-diaminobenzidine chromogen (DAB ...
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bioRxiv - Immunology 2021Quote: ... 5×105 cells were stained for 30 minutes on ice in 50 μl of a labeling mix consisting of anti-IgG2c-AlexaFluor488 (Jackson ImmunoResearch, 115-545-208), anti-IgK-Brilliant Violet421 (BioLegend ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were used at a 1:200 dilution for 30 minutes at room temperature in the dark and include AlexaFluor647 AffiniPure donkey anti-mouse IgG (Jackson ImmunoResearch, 715-605-151), Rhodamine RedX (RRX ...
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bioRxiv - Immunology 2020Quote: ... washed in 5 % FBS/PBS and incubated for a further 30 min with the secondary goat anti-rabbit IgG Alexa-Fluor 488 (Jackson immunoresearch; 111-545-003). Coverslips were mounted in Mowiol (10 % w/v Mowiol4–88 (CalBiochem) ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/30 000) or goat anti-human IgA (α-chain specific) HRP conjugate (1/10 000) (all from Jackson ImmunoResearch Laboratories, Inc.) were added and samples were incubated at 37°C for 1h ...
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bioRxiv - Immunology 2022Quote: ... 5×10 cells were stained for 30 minutes on ice in 50 μl of a labeling mix consisting of anti-IgG2c-AlexaFluor488 (Jackson ImmunoResearch, 115-545-208) and anti-IgK-Brilliant Violet421 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue was dissected to isolate the cochlea and vestibular organs and these were permeabilized for 30 minutes in blocking solution containing PBS with 1% bovine serum albumin (BSA) (Jackson ImmunoResearch 000-001-162) and 5% donkey serum (Jackson ImmunoResearch 017-000-121) ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then washed 5 times with 1% BSA in PBS before being incubated 30 min at room temperature with Alexa Fluor 647 AffiniPure™ Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Europe Ltd., #111-605-003) for Nup133 labeling and Alexa Fluor™ 647-conjugated F(ab’)2-goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS-T and were subsequently treated for 30 min with anti-human Alexa 647–conjugated secondary antibody (Jackson ImmunoResearch; dilution: 1:200 in PBS-T + 2 % BSA) to immunostain the centromeres ...