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Citations for Jackson ImmunoResearch :
851 - 900 of 946 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 2 ug/ml of Goat anti-mouse IgG (H+L) conjugated with Alexa 647 (Jackson ImmunoResearch Laboratories, Inc, Cat# 115-606-146).
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bioRxiv - Cancer Biology 2021Quote: ... STAT-5 (D47E7) XP® followed by secondary antibodies against rabbit IgG (H+L) F(ab’)2 fragment conjugated to DyLight™ 649 (Jackson ImmunoResearch). p-ERK1/2 MFIs in rested effector CD8+ T cells were analysed by flow cytometry.
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bioRxiv - Neuroscience 2020Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch, West Grove, PA). Images were taken with a confocal laser scanning microscope equipped with 473 ...
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bioRxiv - Neuroscience 2020Quote: Free-floating sections were permeabilized and blocked by incubation in 0.1% Triton X-100 and 2% normal donkey serum (NDS; Jackson ImmunoResearch #017-000-121) in PB for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... immunofluorescence staining was performed on deparaffinized and rehydrated tissue sections using a rabbit anti-SARS-CoV-2-NP protein antibody together with an AF488-conjugated anti-rabbit IgG (Jackson ImmunoResearch, PA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:200 and donkey F(ab’)2 anti-human IgM Fc5μ at 1:400 dilution (1 mg/ml, Jackson ImmunoResearch Laboratories, Inc.) was used as the secondary antibody for detection.
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bioRxiv - Immunology 2021Quote: ... at 2 ug/mL and the secondary antibody was Alexa Fluor 647 AffiniPure Donkey Anti-Goat IgG (Jackson Immunoresearch, cat.: 705-605-147) used at 15 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... and HRP-conjugated goat polyclonal anti-human IgG F(ab’)2 fragment antibodies (Jackson ImmunoResearch 109-036-008, 1:5000 dilution in PBSM) were used as secondary detection antibodies for sera and Motavizumab ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch, West Grove, PA). Sections were then mounted on slides (ProLong Gold ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 hours with a secondary anti-rabbit antibody conjugated to Alexa 647 (1:500; Jackson Immunoresearch, 111-605-045) with 5% normal goat serum in PBS + 0.3% Tween-20.
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bioRxiv - Neuroscience 2023Quote: ... and then probed for 2 hours with goat anti-HRP conjugated Alexa Fluor 647 (1:1000, or 0.5 mg/mL, Jackson ImmunoResearch, West Grove, PA). The anti-HRP antibody recognizes a glycoprotein epitope that is present throughout the fruit fly nervous system ...
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bioRxiv - Cell Biology 2023Quote: ... AlexaFluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (cat. #711-546-152, Jackson ImmunoResearch, West Grove, PA, USA); DRAQ5 (cat ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Neuroscience 2024Quote: ... specimens were rinsed 5 times in PBS and treated for 2 h at room temperature in species-specific secondary antibodies conjugated to either DyLight 405 (1:500, Jackson ImmunoResearch Laboratories Inc.), or AlexaFluor-488 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h at room temperature in a solution containing a biotinylated goat anti-rabbit secondary antibody (Jackson Immunoresearch, 1:2000). Sections were washed again and processed with the avidin-biotin complex for 2 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 x 15 min with 0.1 M PBS prior to a 2-hour incubation in secondary antibodies FITC (1:50 in 0.1 M PBS, Jackson ImmunoResearch, 703-095-155), Cy3 (1:50 in antibody diluent ...
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bioRxiv - Pathology 2023Quote: ... and further incubated for 1h at room temperature with cy3 AffiniPure F(ab’)2 fragment rabbit anti-goat IgG and alexa fluor 647 AffiniPure donkey anti-rat IgG secondary antibodies (Jackson ImmunoResearch Europe Ltd). Slides were mounted with Vectashield mounting medium with DAPI (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... and remaining cell surface antibody blocked by incubation with 0.13 mg/ml F(ab’)2 fragment goat-anti rabbit IgG (Jackson Immunoresearch, 111-006-045) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... followed by staining with goat-anti-mouse IgG F(ab’)2-HRP (Jackson ImmunoResearch; 1:5000 diluted in TBS-T with 1% BSA). All incubations were 1h at RT and followed by extensive washing with TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and incubated for 2 h at RT with donkey anti-mouse Cy3 or donkey anti-rabbit Alexa 488 secondary antibodies (Jackson Immunoresearch, Suffolk, UK). Finally ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibody was AffiniPure F(ab’)2 donkey anti-rabbit or donkey anti-mouse IgG (H+L) (711-006-152 and 715-007-003, Jackson ImmunoResearch Europe Ltd) conjugated to the organic fluorophore CF647 (MX647S100 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were then incubated for 2 hours in the secondary antibody solution consisting of Alexa Fluor® 488 donkey anti-chicken (1:250; Jackson ImmunoResearch, AB_2340375), diluted in TBS containing 5% donkey serum ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed (3 × 5mins in 0.01M PBS) and then incubated with secondary antibody for 2 hr (Jackson ImmunoResearch, 1:200 in block). Following washing (3 × 5mins in 0.01M PBS) ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS at 400 × g 5 minutes and then stained with FITC-conjugated AffiniPure donkey or Cy5-conjugated AffiniPure donkey anti-mouse 2nd Abs (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at 1:100 dilution for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human serum IgA (α-chain specific) at 0.3 μg/mL or goat anti-human IgG (γ-chain specific) at 5 μg/mL (all from Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Microtiter plates were sealed and stored overnight at 2-8°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and blocked with 100 μL blocking buffer (1% bovine serum albumin and 5% normal donkey serum [Jackson ImmunoResearch, cat 017-000-121, lot 152961] in PBST) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 10uL of HRP-fused human anti-IgG (IgG#5 by NRC, 0.9 ng/well) or HRP-conjugated human anti-IgA (Jackson ImmunoResearch, #109-035-127, 0.8 ng/well) diluted with 1% Blocker BLOTTO in PBS-T was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... followed by washing with PBS and labelling with a secondary antibody (Cy™5 AffiniPure conjugated polyclonal Donkey Anti-Mouse IgG; Jackson ImmunoResearch Laboratories, #715-225-150) (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). Sections were then washed and processed using 4”,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biophysics 2021Quote: ... Transverse 50 μm sections were obtained using Leica microtome and stained using mouse monoclonal β-tubulin antibody (E7, DSHB; 1-2 μg/ml) followed by secondary goat anti-mouse Cy3 antibody (Jackson ImmunoResearch; 1:300). To visualize actin filaments in embryos microinjected with 0.5 μm nanoparticles ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in biotin-conjugated donkey anti-rabbit secondary antibody (1:1000 in 2% BSA and PBS, #711-065-152, Jackson ImmunoResearch, Cambridgeshire, United Kingdom) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were light-protected and incubated at room temperature for 2 h with donkey anti- rabbit IgG AlexaFluor®488-conjugated (1:400; Jackson ImmunoResearch, Pennsylvania, USA) and Streptavidin AlexaFluor®594-conjugated (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-ACK2 primary antibodies were incubated for 2 h in biotinylated secondary antiserum (anti-rabbit 1:2500 or anti-rat, 1:1000; Jackson ImmunoResearch, West Grove, PA) specific to the species of the primary antibodies and were constituted in PBS with 0.32% TX ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). In the concluding steps of the staining protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (Jackson ImmunoResearch Cat# 715-586-151, RRID:AB_2340858) antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (Jackson ImmunoResearch Cat# 711-586-152, RRID:AB_2340622). After several rinses in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Frozen VQ- D2 donor cells were gently thawed at 37°C before being washed twice in PBS containing 2% mouse serum (Jackson ImmunoResearch, #015-000-120). Donor cells were then resuspended in 200 μl of PBS containing 2% mouse serum ...
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bioRxiv - Neuroscience 2022Quote: ... then treated for 2 hours in a PBS solution containing 0.3% TritonX-100 and 10% normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, West Grove, PA). Sections were then treated overnight at 4°C with a PBS solution containing 1% NDS ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed again in PBS-triton and incubated for 2 hours with the secondary antibodies at 1:1000 dilution (Alexa Fluor 488 anti-chicken (Jackson ImmunoResearch 703-545-155) and Alexa Fluor 568 anti-rabbit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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Localization of Cadherins in the postnatal cochlear epithelium and their relation to space formationbioRxiv - Cell Biology 2024Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...