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Citations for Jackson ImmunoResearch :
751 - 800 of 814 citations for tert Butyl 2 chloro 5H pyrrolo 3 4 b pyridine 6 7H carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibody was AffiniPure F(ab’)2 donkey anti-rabbit or donkey anti-mouse IgG (H+L) (711-006-152 and 715-007-003, Jackson ImmunoResearch Europe Ltd) conjugated to the organic fluorophore CF647 (MX647S100 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were then incubated for 2 hours in the secondary antibody solution consisting of Alexa Fluor® 488 donkey anti-chicken (1:250; Jackson ImmunoResearch, AB_2340375), diluted in TBS containing 5% donkey serum ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). Sections were then washed and processed using 4”,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biophysics 2021Quote: ... Transverse 50 μm sections were obtained using Leica microtome and stained using mouse monoclonal β-tubulin antibody (E7, DSHB; 1-2 μg/ml) followed by secondary goat anti-mouse Cy3 antibody (Jackson ImmunoResearch; 1:300). To visualize actin filaments in embryos microinjected with 0.5 μm nanoparticles ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in biotin-conjugated donkey anti-rabbit secondary antibody (1:1000 in 2% BSA and PBS, #711-065-152, Jackson ImmunoResearch, Cambridgeshire, United Kingdom) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were light-protected and incubated at room temperature for 2 h with donkey anti- rabbit IgG AlexaFluor®488-conjugated (1:400; Jackson ImmunoResearch, Pennsylvania, USA) and Streptavidin AlexaFluor®594-conjugated (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-ACK2 primary antibodies were incubated for 2 h in biotinylated secondary antiserum (anti-rabbit 1:2500 or anti-rat, 1:1000; Jackson ImmunoResearch, West Grove, PA) specific to the species of the primary antibodies and were constituted in PBS with 0.32% TX ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). In the concluding steps of the staining protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (Jackson ImmunoResearch Cat# 715-586-151, RRID:AB_2340858) antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated in secondary Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (Jackson ImmunoResearch Cat# 711-586-152, RRID:AB_2340622). After several rinses in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Frozen VQ- D2 donor cells were gently thawed at 37°C before being washed twice in PBS containing 2% mouse serum (Jackson ImmunoResearch, #015-000-120). Donor cells were then resuspended in 200 μl of PBS containing 2% mouse serum ...
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bioRxiv - Neuroscience 2022Quote: ... then treated for 2 hours in a PBS solution containing 0.3% TritonX-100 and 10% normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, West Grove, PA). Sections were then treated overnight at 4°C with a PBS solution containing 1% NDS ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed again in PBS-triton and incubated for 2 hours with the secondary antibodies at 1:1000 dilution (Alexa Fluor 488 anti-chicken (Jackson ImmunoResearch 703-545-155) and Alexa Fluor 568 anti-rabbit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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Localization of Cadherins in the postnatal cochlear epithelium and their relation to space formationbioRxiv - Cell Biology 2024Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...
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bioRxiv - Neuroscience 2024Quote: ... washed 3 times in PB 0.1 M, a secondary antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, goat anti-rabbit Cyanine Cy3TM (Jackson Immunoresearch, 111-165-003, RRID: AB_2338000), goat anti-mouse Cy5TM (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 15 minutes on ice with 100 mL of 1:500 APC-F(ab’)2 fragment goat anti-human IgG (Jackson Immunoresearch #109-136-098) diluted in FACS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in secondary antibody solution (0.1% Triton 100X in PBS) for 2 hours at room temperature with the following secondary antibodies: Cy3 donkey anti-rabbit IgG (1:1000, Jackson ImmunoResearch, #711-165-152) and Cy5 donkey anti-guinea pig IgG (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... blocked tissues overnight with blocking buffer and stained with a polyclonal goat anti-mouse F(ab’)2 fragment (1:100; Jackson ImmunoResearch 115-005-072) and rabbit anti-mouse NF200 (1:100 ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... yeast were washed in PBS/0.2% BSA and rotated at 18 rpm in 300 μL of same buffer containing 2 μg/mL Alexa488-conjugated goat anti-chicken IgG (Jackson ImmunoResearch, West Grove, PA) for one hour at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and then incubated for 2 h at room temperature with Alexa 488 goat anti mouse 1:1000 (Jackson ImmunoResearch, cat n° 115-545-166), Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... BCR-mediated Ca2+ mobilization was measured for 60s after the cells were stimulated either with 10 μg/mL F(ab′)2 of polyclonal goat anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA, USA) or 0.5mM phosphatidylcholine (PtC ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 2 hours at room temperature in Alexafluor-488 conjugated donkey anti-chicken (1:500; Jackson ImmunoResearch 703-546-155, lot# 142461) in 1% goat serum and 0.3% Triton X100 in PBS ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of red-phycoerythrin (R-PE) conjugated F(ab’)2 fragment goat anti-mouse IgG specific to the Fc fragment (Jackson ImmunoResearch, Inc., West Grove, PA) were added at 1 μg/ml to the wells and incubated for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using goat-anti-human IgG-Alexa fluor-488 or anti-human IgG F(ab’)2-Alexa fluor-488 (Jackson ImmunoResearch, West Grove, PA, USA), and mean cellular fluorescence was determined using flow cytometry (Intellicyt iQue ...
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bioRxiv - Cancer Biology 2023Quote: ... washed thrice with PBS and then stained with goat anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 647 (Jackson ImmunoResearch Labs; West Grove, PA) to monitor for p-STAT-5 in CD8+ OT-I cells by flow cytometry.
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-goat or anti-mouse biotinylated secondary antibodies were incubated for 2 hours at room temperature (1:1000, Jackson ImmunoResearch, West Grove, PA, USA), followed by incubation with VECTASTAIN Elite ABC HRP solution (Vector ...
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat#: 715-116-150) using flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... 14 Slides were then incubated with 1 µg/mL biotinylated donkey F(ab’)2 anti-rabbit (711-066-152, Jackson ImmunoResearch Laboratories, West Grove, PA) secondary antibodies for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Immunology 2023Quote: ... or 10 μg/ml AF-488/−647 labelled F(ab’)2 Fragments of donkey anti-mouse IgM (Jackson ImmunoResearch 715-546-020/715-606-020), were placed in 300 μl of imaging buffer (10% FCS in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with secondary antibodies at room temperature for 2 hours: Alexa Fluor 488 AffiniPure donkey anti-rabbit IgG(H+L) (1:500, Jackson immunoresearch, PN-712-545-152), Alexa Fluor 647 AffiniPure donkey anti-rabbit IgG(H+L ...
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bioRxiv - Immunology 2024Quote: ... 5% CO2 with 25 µl of secondary antibody alkaline phosphatase conjugated F(ab’)2 goat anti-mouse IgG Fc-gamma specific (Jackson Immunoresearch, USA, # 115-056-071) diluted 1/3000 in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... and MYC-tag was detected with 100 µl per well of HRP-F(ab)2 fragment goat anti-mouse IgG (H+L) (Jackson ImmunoResearch, Cat #115-036-062) at 1/ 5000 in the same binding solution ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed three times and then incubated 2 h rocking at RT with the secondary antibody goat anti-rabbit Cy3 (Cy3, 1:200, Jackson ImmunoResearch, West Grove, PA, USA). Brain slices were finally mounted on microscope slides using DAPI Fluoromount-G (SouthernBiotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunohistochemistry for dsRed was performed with a 7 minute −20°C 100% acetone target retrieval and blocking in 2% normal goat serum (Jackson ImmunoResearch, cat. no. 005-000-121). Primary antibodies include rabbit anti-mCherry (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 h at room temperature in a solution containing a mixture of goat-Cy3 anti-rabbit (1:500, Jackson ImmunoResearch Laboratories, Cat. #111–165–144), goat anti-chicken Alexa 647 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 3X with FACS buffer and stained with secondary antibody Cy3-conjugated Goat Anti-mouse IgG (Jackson ImmunoResearch, 2 μg/mL in FACS buffer) for 1 hour at RT ...