Labshake search
Citations for Jackson ImmunoResearch :
751 - 800 of 802 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 0.1 μg/ml of Alexa Fluor 488-conjugated donkey polyclonal anti-mouse IgG anti-body (Jackson ImmunoResearch, West Grove, PA, USA) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Organs were thawed with chicken saline with 1:100 Protease Inhibitor Cocktail and 2% normal donkey serum (NDS; Jackson ImmunoResearch, West Grove, PA, USA; Cat# 017-000-121) at ∼5 ml per 100 ears ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Immunology 2024Quote: ... IgG reactivity was detected by adding 50 μL/well R-Phycoerythrin-(PE)-conjugated AffiniPure F(ab’)2 Fragment Goat Anti-Mouse antibody (Jackson ImmunoResearch, Code Number: 115-116-146, 1:3500) following incubation and washes ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse IgG was detected using Cy™3 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Cat# 715-166-150; 1:500 dilution) and rabbit IgG was detected using Cy™5 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Immunology 2020Quote: ... A standard curve was applied to each plate starting with 100 µl of 50 ng/ml (015-000-003, Jackson ImmunoResearch, USA) and two-fold serially diluted down ...
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bioRxiv - Cell Biology 2021Quote: ... 80 µL of a 1.25 mg/mL suspension of unlabeled goat anti-mouse IgG1 secondary antibody (115-005-205, Jackson ImmunoResearch Laboratories, Inc.), and 10 µL of 1M sodium bicarbonate solution were mixed and allowed to react for 15 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Labelled cells were seeded at 5×104 cells per well and cultured for 90 hours with either AffiniPure F(ab’)₂ Fragment Goat Anti-Mouse IgM µ Chain Specific (20mg/ml; Jackson Immunoresearch), CD40L (produced in-house as described 63 ...
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bioRxiv - Physiology 2021Quote: ... followed by a secondary donkey anti-rabbit Ig antibody coupled to Alexa 488 (3 μg/ml, Jackson ImmunoResearch Laboratories, West Grove, PA). A goat anti-Aqp2 antibody (0.2 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight with 250 ng/well of purified goat anti-human IgA or IgG antibody (Jackson ImmunoResearch, 0.8 µg/ml final). After washings ...
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bioRxiv - Immunology 2019Quote: ... and for 3 min after the addition of cross-linking antibody goat anti-Armenian Hamster IgG (50 μg/mL; Jackson Immunoresearch Laboratory), followed by ionomycin (1 μM) ...
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently the coverslips were washed three times in wash buffer (0.05% Tween-20 in PBS) for 5 minutes and blocked for 20 minutes in 200 ml wash buffer plus 5% normal donkey serum (Jackson ImmunoResearch Laboratories). The primary antibodies were diluted in 5% normal donkey serum in PBS and incubated with the tissues 1 hour at room temperature followed by three washes in wash buffer for 5 minutes each ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Immunology 2023Quote: ... A20 D1.3 or primary B cells were labelled on ice for 10 minutes with 10 μg/ml of Alexa Fluor® 647 donkey anti-mouse IgM (#715-605-140, Jackson ImmunoResearch) or RRx goat anti-mouse IgM (#115-295-205 ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... A20 D1.3 or primary B cells isolated from MD4 mice spleens were labelled on ice for 10 minutes with 10 μg/ml of Alexa Fluor® 647 donkey anti-mouse IgM (#715-605-140, Jackson ImmunoResearch) or RRx goat anti-mouse IgM (#115-295-205 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.01% SDS with 1× protease inhibitor cocktail) and then blocked with 200 µl 20 mg/ml BSA (Jackson ImmunoResearch, 001-000-161), 4 µl 10 mg/ml salmon sperm DNA (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS-T and were subsequently treated for 30 min with anti-human Alexa 647–conjugated secondary antibody (Jackson ImmunoResearch; dilution: 1:200 in PBS-T + 2 % BSA) to immunostain the centromeres ...
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bioRxiv - Molecular Biology 2020Quote: ... modified with a 1:1 volume ratio of packed hydrogel spheres and 1 mg/ml streptavidin-rhodamine (Jackson ImmunoResearch, Cat # 016-290-084) for 5 min ...
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bioRxiv - Biophysics 2019Quote: ... plasma membrane-localized BCR was labeled for 10 min at room temperature with 5 mg/mL goat anti-mouse IgM (Jackson ImmunoResearch; RRID: AB_2338477) f(Ab)1 fragments conjugated to both silicon rhodamine (SiR ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was routinely washed and incubated with the sheep anti-rabbit secondary antibodies (1 mg/ml) conjugated with horseradish peroxidase (Jackson ImmunoResearch Laboratories, USA) for 2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-170) and goat anti-human IgA-Cy3 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding to the beads was detected with 2.5 µg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170), goat anti-human IgA-Cy3 (Jackson Immunoresearch 109-166-011 ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Immunology 2020Quote: ... 6.6 μg/ml)) and a suitable fluorescence (Cy3)-labelled secondary antibody (AffiniPure Donkey Anti-Sheep IgG, Minimal Cross Reactions, Jackson Immunoresearch, Cambridge, UK) for 2 and 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Bound IgG was detected using 1μg/mL red phycoerythrin (R-PE)-conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, Inc., West Grove, PA) and resuspended in MAGPIX® drive fluid before being analyzed on a MAGPIX® Instrument (Luminex Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated with Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1.9 µg/ml; Jackson ImmunoResearch, cat #111-165-003) for standard epifluorescence assays ...
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bioRxiv - Immunology 2023Quote: ... After that they were seeded in the 12-well slides coated with 10μg/ml F(ab’) Fragment Goat Anti-Mouse IgM (Jackson ImmunoResearch, 115-006-020) in PBS for 1 hour at room temperature and activated for 15 or 5 min in the incubator ...
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bioRxiv - Immunology 2019Quote: ... The cells were then placed on ice and labeled for 5 min with 1 ng/ml Cy3-conjugated anti-mouse IgM Fab fragments (Jackson ImmunoResearch, #115-167-020), 100 ng/ml Cy3-conjugated Fab fragments from the 11-26c rat anti-mouse IgD monoclonal antibody (ATCC ...
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bioRxiv - Developmental Biology 2019Quote: ... after several rinses in TBS corneas were blocked with goat anti-mouse IgG Fab fragments (Jackson ImmunoResearch; 0.13 mg/ml; 1 h; room temperature) prior to overnight incubation at 4°C in mouse anti-ZO-1 (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... or Neutralizing Antibody isolated from COVID-19 patients for 15 min at 4 °C then washed with PBS and stained again in 50 μL of the anti-Rabbit Alexa 647 (7,5μg/ml, Jackson ImmunoResearch Cat. No. 111-606-144) or anti-human IgG PE/ Dazzle™ 594 (2μg/ml ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 μl of 5 μg/ml HIV-1 Env gp140 SF162.B (NIH AIDS Reagent Program) or 1 μg/ml anti-human IgG or IgM (Jackson Immunoresearch, West Grove, PA) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature [6-8,10] ...
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bioRxiv - Immunology 2023Quote: ... containing human IgG1 constant regions were tested at 4µg/ml and at three four-fold dilutions and were detected with peroxidase-conjugated goat anti-human IgG Fc (Jackson ImmunoResearch, 109-035-098). Peroxidase was revealed with 1-Step ABTS substrate solution (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Residual rabbit immunoreactivity was subsequently blocked overnight with unlabeled donkey anti-rabbit Fab fragment (AffiniPure Fab Fragment Donkey Anti-Rabbit IgG (Jackson ImmunoResearch, 26 μg/ml). Tissues were rinsed with PBS-T and incubated with the second primary antibody raised in rabbit (DCLK1 or neurogranin) ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-or 488-conjugated donkey polyclonal anti-rabbit or anti-mouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells immunostained for E-cadherin with Alexa Fluor 488 secondary antibody were blocked with 26 µg/ml of AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Inc, West Grove, PA) in 5% NGS in PBS overnight before being stained for LAMP1 with Alexa Fluor 594-conjugated goat anti-mouse secondary antibody (Invitrogen).
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bioRxiv - Bioengineering 2021Quote: ... wells were incubated with 100 µL 1/2500 dilution (320 ng/mL) of goat anti- rabbit:HRP (Jackson ImmunoResearch, West Grove, PA, Cat#111-035-144) in PBSCT for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml; Jackson ImmunoResearch#715-175-150, RRID AB_2340819) and the Cy3-conjugated goat anti-rabbit antibody (1.9 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with cells at 250 ng/mL for at least 48 hours in combination with an anti-mouse IgG secondary antibody from Jackson Immunoresearch (catalog #115-006-071) at 1 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-human IgM (Jackson Immunoresearch Affinipure; 0.6-75 ug/mL), or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure ...
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bioRxiv - Cell Biology 2021Quote: ... with 20 μg/ml Affinipure Fab fragment goat anti-mouse IgG (H+L) (catalog number 115-007-003, Jackson ImmunoResearch Laboratories, Inc. West Grove, PA, USA). The following primary antibodies were used ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation with 1 µg/ml of recombinant sE WT or dimer-mutants or anti-human IgG-HRP (Jackson ImmunoResearch, 109-035-088, 1:5000) in PBS+0.5% FCS for 2 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... before resuspending in 25 μl of 0.2 µm-filtered PBS containing polyclonal Alexa Fluor 647 Rabbit Anti-Mouse IgG (Jackson ImmunoResearch, 15 µg/ml, 315-605-003, AB_2340239) or monoclonal Brilliant Violet 421 Rat Anti-Mouse IgA (BD Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with primary antibodies (see Key Resources Table) overnight and subsequent incubated with secondary antibodies labeled with Alexa Fluor 488- (Jackson ImmunoResearch, 115-545-003, 3 μg/mL) or Cy3- (Jackson ImmunoResearch ...