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Citations for Jackson ImmunoResearch :
7001 - 7050 of 7113 citations for Anti NEK9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Immunopurification was carried out using hydrophobic petri dishes coated overnight with Biotin-SP-conjugated AffiniPure goat anti-rabbit IgG (1:1000; Jackson ImmunoResearch, West Grove, PA) in 50 mM Tris buffer at 4°C followed by another overnight incubation at 4°C with either mouse anti-Ngfrp75 for rat OECs (Suppl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The following secondary antibodies were used: Rhodamine Red™-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:200; Cat.#711-295-152; Jackson ImmunoResearch Laboratories, Inc.); Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were goat anti-guinea pig IgG (1: 200, Alexa Fluor 488 conjugated, Jackson ImmunoResearch, cat # 106545003; 1:200, Alexa Fluor 647 conjugated, Jackson ImmunoResearch, cat # 106605003).
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for 1 hour at room temperature with goat anti-mouse IgG horseradish peroxidase (HRP) (Jackson ImmunoResearch, 115-035-166): IgG1 and IgG2a/b levels were quantified using goat anti-mouse IgG1 HRP (Southern Biotechnology Associates ...
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bioRxiv - Neuroscience 2023Quote: ... They were rinsed in PBS three times (10 minutes each) and incubated in Cy3-conjugated donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch #711-165-152) with 2% NDS and 0.3% TX for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were light-protected and incubated at room temperature for 2 h with donkey anti- rabbit IgG AlexaFluor®488-conjugated (1:400; Jackson ImmunoResearch, Pennsylvania, USA) and Streptavidin AlexaFluor®594-conjugated (1:200 ...
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bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Pathology 2023Quote: ... Alexa Fluor 488 AffiniPure F(ab’)[Fragment Donkey Anti-Goat IgG (H+L) (1:200, 705-546-147, Jackson ImmunoResearch, West Groove, PA, USA), Donkey anti-Goat IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-Mon1a and Fcho2 were used at a concentration of 1:500 followed by peroxidase conjugated goat anti-rabbit IgG (1:10,000) (Jackson ImmunoResearch Labs, West Grove, PA). The blots were developed using Western Lightning reagent (PerkinElmer Life Sciences ...
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bioRxiv - Bioengineering 2023Quote: ... an ELISA assay was developed to detect LH01 or un-cleaved LH05 with IL-15Rβ coated on the plate and Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) as the detection antibody ...
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bioRxiv - Neuroscience 2023Quote: ... They were rinsed in PBS three times (10 minutes each) and incubated in Cy3-conjugated donkey anti-rabbit IgG (1:400; Jackson ImmunoResearch #711-165-152) with 2% NDS for 90 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... containing human IgG1 constant regions were tested at 4µg/ml and at three four-fold dilutions and were detected with peroxidase-conjugated goat anti-human IgG Fc (Jackson ImmunoResearch, 109-035-098). Peroxidase was revealed with 1-Step ABTS substrate solution (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Immunology 2023Quote: ... which was visualized with a goat anti-rabbit IgG conjugated with FITC (green, cat#111-225-144, Jackson ImmunoResearch Laboratories, INC., West Grove PA). The DNA dye Hoechst 3328 (blue ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was washed 3 times in TBST-T at room temperature for 15 minutes each wash followed by incubation with secondary polyclonal anti-rabbit antisera HRP for one hour at room temperature in TBS-T (Jackson ImmunoResearch, 111-035-003). After 3 additional washes with TBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were washed three times in PBS and stained with goat-anti-rabbit Alexa Fluor 647 Fab Fragment (1:800; Jackson ImmunoResearch 111-607-003) for an hour ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with 6 nm Colloidal Gold AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 115-195-146, 1:20, AB_2338728) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed 3X 15 min in 0.1M PBS and then incubated at 4°C for 24 h with donkey anti-chicken-488 (1:1000; 703-545-155, Jackson Immunoresearch, West Grove, PA) and donkey anti-rabbit-CY3 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the appropriate mixture of the secondary donkey anti-rabbit Cy3 (1:700; 711-165-152, Jackson Immunoresearch, West Grove, PA) and donkey anti-chicken-488 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2024Quote: ... rat 70 kDa isoform) (custom-made, as described [66], and mTOR P-S1261 (custom-made, as described [66]. Donkey anti-rabbit-HRP (Jackson ImmunoResearch, 711-095-152) detected ECL-based western blot signals ...
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bioRxiv - Cancer Biology 2024Quote: ... AffiniPure goat anti-mouse IgG (H+L) (0.4 µg/mL in PBS supplemented with 1% w/v BSA; Jackson ImmunoResearch, West Grove, PA, USA) was added ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X for 10 min each in T-Buffer followed by one wash in TBS and incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, diluted at 1:250) and Alexa Fluor 488-conjugated donkey anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2019Quote: ... and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L) (1: 50 dilution; 111-295-003; Jackson ImmunoResearch Europe Ltd, Cambridge, UK) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... rat anti-DEcadherin (clone DCAD2 from Developmental Studies Hybridoma Bank at the University of Iowa) 1:25 and Donkey anti-rat Cy2 (Jackson ImmunoResearch Laboratories, West Grove, PA) at 1:400 ...
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bioRxiv - Physiology 2019Quote: ... The antibody-antigen complexes were visualized by incubation for 1 hour at room temperature with 1:2000 Alexa Fluor 488-conjugated goat anti-rabbit (Molecular Probes A11008/Jackson ImmunoResearch, 111-485-144) and Cy5-conjugated donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2019Quote: ... antibody and detected with 1:200 anti-rabbit CY3 (Jackson Immunoreserach, cat# 715-165-151) or 1:200 Alexa 488 Mouse (1:200, Jackson Immunoresearch, cat# 715-545-150) secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and then incubated for 2 h at room temperature with Alexa 488 goat anti mouse 1:1000 (Jackson ImmunoResearch, cat n° 115-545-166), Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Microbiology 2021Quote: ... Slides were washed three times with TBS buffer with 0,05 % Tween-20 and further incubated with anti-rabbit Alexa 647 (Jackson ImmunoResearch Cat. No. 111-606-144), anti-human IgG-Alexa647 (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: ... overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003, Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... BCR-mediated Ca2+ mobilization was measured for 60s after the cells were stimulated either with 10 μg/mL F(ab′)2 of polyclonal goat anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA, USA) or 0.5mM phosphatidylcholine (PtC ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated for 90 min at room temperature in Alexa Fluor 488- or 594-conjugated donkey anti-mouse IgG (1:250; Jackson ImmunoResearch; RRID: AB_2313584; RRID: AB_2340621) in PBS with 0.5% Triton X-100 and 2% normal donkey serum ...
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bioRxiv - Bioengineering 2021Quote: ... wells were incubated with 100 µL 1/2500 dilution (320 ng/mL) of goat anti- rabbit:HRP (Jackson ImmunoResearch, West Grove, PA, Cat#111-035-144) in PBSCT for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... washed twice with PBSB by centrifugation for 5 min at 1200 rpm and then incubated for an additional hour with 50 µL of R-Phycoerythrin AffiniPure F(ab’)₂ Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, Cat#109-116-170) at 250 ng/mL diluted in PBSB ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 2 hours at room temperature in Alexafluor-488 conjugated donkey anti-chicken (1:500; Jackson ImmunoResearch 703-546-155, lot# 142461) in 1% goat serum and 0.3% Triton X100 in PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted washed twice with PBSA and subsequently labeled with PE-conjugated goat anti-human IgG (1:100, Jackson ImmunoResearch Labs #109-115-098) and FITC-conjugated chicken anti-cMmyc (1:100 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of red-phycoerythrin (R-PE) conjugated F(ab’)2 fragment goat anti-mouse IgG specific to the Fc fragment (Jackson ImmunoResearch, Inc., West Grove, PA) were added at 1 μg/ml to the wells and incubated for 45 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... All secondary antibodies were used at 1:5,000 dilutions as follows: Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (Jackson ImmunoResearch Laboratories; #703-545-155); Donkey anti-Rabbit IgG (H+L) ...
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bioRxiv - Immunology 2022Quote: ... 14 Slides were then incubated with 1 µg/mL biotinylated donkey F(ab’)2 anti-rabbit (711-066-152, Jackson ImmunoResearch Laboratories, West Grove, PA) secondary antibodies for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HRP-conjugated anti-rabbit IgG (1:5000, NA934V, Cytiva, Tokyo, Japan) or HRP-conjugated anti-mouse IgG (1:10000, 115-035-003, Jackson Immunoresearch, West Grove, PA, USA). Note that STAT6 was immunoblotted after stripping the pSTAT6 signal by using WB Stripping Solution Strong (Nacalai Tesque ...