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Citations for Jackson ImmunoResearch :
651 - 700 of 865 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... an ELISA assay was developed to detect LH01 or un-cleaved LH05 with IL-15Rβ coated on the plate and Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) as the detection antibody ...
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bioRxiv - Immunology 2023Quote: ... containing human IgG1 constant regions were tested at 4µg/ml and at three four-fold dilutions and were detected with peroxidase-conjugated goat anti-human IgG Fc (Jackson ImmunoResearch, 109-035-098). Peroxidase was revealed with 1-Step ABTS substrate solution (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then treated with blocking buffer containing 5% normal goat serum (Jackson Immunoresearch, 005-000-121), 0.1% Tween-20 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue sections were washed with PBS and blocked with 5% donkey or goat serum (Jackson ImmunoResearch), 2% BSA (Gemini Bio-Products ...
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bioRxiv - Microbiology 2019Quote: ... the permeabilisation solution was exchanged with 5% normal goat serum (#005-000-121, Jackson ImmunoResearch Laboratories, Inc.) in 0.05% PBS-Tween20 ...
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bioRxiv - Neuroscience 2020Quote: ... CY™5-conjugated donkey anti-rabbit (1/500, v/v; CAT#711-175-152, Jackson ImmunoResearch), and Brilliant Violet™421-conjugated donkey anti-goat (1/500 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 90 min in HRP-linked secondary antibody (Jackson ImmunoResearch; 1:10,000 in TBST + 5% BSA), then washed three times with TBST and twice with TBS for 10 min each ...
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bioRxiv - Developmental Biology 2020Quote: Samples were blocked in dPBS with 5% BSA and 1% donkey serum (Jackson Immunoresearch, 017-000-001). Concentration of primary antibodies and % Triton X-100 are listed in the Supplementary table 2 ...
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bioRxiv - Physiology 2022Quote: ... Tissue sections of 10 μM were blocked in 5% Normal Goat Serum (Jackson ImmunoResearch, West Grove, PA) in PBS + 1%BSA + 0.3%Triton X-100 for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and blocked with PBS+5% BSA (Fisher) or serum of the host of secondary antibodies (Jackson ImmunoResearch). Coverslips were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies (diluted 1:300) were diluted into 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody for flow cytometry we used PE-conjugated goat anti-human IgG + IgM (H+L) (F(ab’)2) (Jackson Immunoresearch, no. 109-116-127).
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and the binding of VHH-Fcs to denatured S was probed by incubating strips with 1 mL of 100 ng/mL anti-human Ig Fc antibody-peroxidase conjugate (Jackson ImmunoResearch, Cat#016-030-084) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... washed twice with PBSB by centrifugation for 5 min at 1200 rpm and then incubated for an additional hour with 50 µL of R-Phycoerythrin AffiniPure F(ab’)₂ Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, Cat#109-116-170) at 250 ng/mL diluted in PBSB ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted washed twice with PBSA and subsequently labeled with PE-conjugated goat anti-human IgG (1:100, Jackson ImmunoResearch Labs #109-115-098) and FITC-conjugated chicken anti-cMmyc (1:100 ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 µL of FACS buffer containing secondary R-PE Goat Anti-Human IgG antibody diluted 1:100 (Jackson ImmunoResearch, cat #109-117-008), and incubated for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Total IgG was measured following 6 washes and a 1-h incubation with 100 μL/well of 1:50,000 HRP-conjugated polyclonal goat anti-human IgG (Jackson ImmunoResearch Labs, West Grove, PA, USA). IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech ...
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bioRxiv - Immunology 2023Quote: ... Grids were washed several times with PBS and incubated with 12-nm gold-labeled anti-human secondary antibody (Jackson ImmunoResearch, diluted 1:40 in PBS) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... wells were washed 6 times and 1:5000 dilution of horseradish peroxidase (HRP)-conjugated donkey anti-human IgG (FC specific; Jackson ImmunoResearch Laboratories, West Grove, PA) was added in blocking solution to each well and the plate was incubated for 1 hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The blockage of nonspecific sites was performed by 1% BSA and 5 μg/mL donkey-IgG (Jackson ImmunoResearch) in PBS for 45min ...
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bioRxiv - Bioengineering 2019Quote: ... and blocking for 30 min in 3 to 5 % Bovine Serum Albumin (Jackson ImmunoResearch, West Grove, PA, USA) or 10 % goat serum in phosphate-buffered saline with 0.05 to 0.1% Triton-X 100 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were permeabilized and blocked simultaneously in PBS containing 0.1% Triton and 5% normal goat serum (Jackson Immunoresearch) for 2 hr at 4°C followed by incubation with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... then permeabilized and blocked with 0.5% Tween-20 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were permeabilized and blocked simultaneously in PBS containing 0.1% Triton and 5% normal goat serum (Jackson Immunoresearch) for 1 h at room temperature followed by incubation with primary antibodies overnight at 4°C ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Cyanine Cy™5-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 711-175-152; IF 1:400) and Cyanine Cy™5-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... brains were blocked for 30 min in PBS supplemented with 5% normal donkey serum (Jackson Immunoresearch (PA, USA)) and 0.3% Triton X-100 (Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum (cat# 017-000-121, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hr ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS before blocking in 5% normal goat serum (Jackson ImmunoResearch 005-000-121; NGS) with 0.05% Triton X-100 (Sigma #T9284100 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then blocked with 5% NGS/PBS and incubated with rhodamine (TRITC) streptavidin (1:400, Jackson ImmunoResearch Labs Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... The sections were subsequently incubated in the blocking buffer of 5% normal donkey serum (Jackson ImmunoResearch, Cat: 017000121) or 10% normal goat serum (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Cy™5 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (706-175-148) were from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Physiology 2023Quote: ... Un-specific binding was blocked with 5% bovine serum albumin and 10% donkey serum (Jackson ImmunoResearch, 017-000121) in 1X PBS for 60 min ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were blocked in 5% goat or horse serum (#005-000-121, #008-000-121, Jackson ImmunoResearch) with 0.2% Triton X-100 and incubated with anti-pS129-α-syn antibodies (#ab184674 ...
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bioRxiv - Cancer Biology 2024Quote: ... fixed cells were incubated with PBS solution containing 5% donkey serum (Jackson ImmunoResearch Labs, West Grove, PA, USA) and 0.2% Triton X-100 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 5 minutes each and were incubated in secondary antibodies (donkey anti-chicken cy3 (Jackson Immunoresearch, 703-165-155); donkey anti-rat Alexa 488 (Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... Next, the secondary antibody (Cy™5 Donkey Anti-Rabbit IgG, 1:400) (Jackson ImmunoResearch, 711-175-152) was added for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-human IgM (Jackson Immunoresearch Affinipure; 0.6-75 ug/mL), or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure ...
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bioRxiv - Cell Biology 2021Quote: External beads were stained for live-cell imaging by incubating macrophages with Cy2-conjugated anti-human IgG (1:100; Jackson ImmunoResearch Labs, West Grove, PA, USA) for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed with PBS and incubated with allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (Jackson ImmunoResearch Laboratories, cat# 709-136-149) at a final concentration of 62.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... or unconjugated anti-human CCL19 (polyclonal, R&D AF361-SP, 1:200) that was detected with anti-goat IgG A488 (Jackson Immunoresearch 705-545-003, 1:1000) and unconjugated anti-human PDPN (Clone NZ-1.3 ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were washed twice with filtered PBS and then resuspended in 0.2 µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG (Jackson ImmunoResearch, 1:100, 109-605-098, AB_2337889) and Brilliant Violet 421-anti-rabbit IgG (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation with 1 µg/ml of recombinant sE WT or dimer-mutants or anti-human IgG-HRP (Jackson ImmunoResearch, 109-035-088, 1:5000) in PBS+0.5% FCS for 2 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... wherein fixed CDMs and/or cells were blocked in 5% normal donkey serum (017-000-121, Jackson Immunoresearch Labs) and 0.3% Triton-X 100 (T8787 ...
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bioRxiv - Developmental Biology 2021Quote: ... After sequential blocking for each 1 h in 5% donkey and fetal calf serum (Jackson Immunoresearch, Ely, United Kingdom) and 0.5% Triton X-100 (Merck) ...